LOCUS       40924_48118        7993 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Neurospora expression vector pYFPC, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7993)
  AUTHORS   Bardiya N, Alexander WG, Perdue TD, Barry EG, Metzenberg RL, Pukkila
            PJ, Shiu PK.
  TITLE     Characterization of interactions between and among components of the
            meiotic silencing by unpaired DNA machinery in Neurospora crassa 
            using bimolecular fluorescence complementation
  JOURNAL   Genetics 178 (1), 593-596 (2008)
  PUBMED    18202398
REFERENCE   2  (bases 1 to 7993)
  AUTHORS   Shiu PKT.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2007) Division of Biological Sciences, University 
            of Missouri-Columbia, 103 Tucker Hall, Columbia, MO 65203, USA
REFERENCE   3  (bases 1 to 7993)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7993)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics"; 
            date: "2008"; volume: "178"; issue: "1"; pages: "593-596"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-JUN-2007) Division of Biological Sciences, University of 
            Missouri-Columbia, 103 Tucker Hall, Columbia, MO 65203, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7993
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        363..467
                     /label=AmpR promoter
     CDS             468..1325
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1499..2087
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2345..2363
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2638..2649
                     /codon_start=1
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
     primer_bind     4981..4997
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             5034..5285
                     /codon_start=1
                     /label=VC155
                     /note="C-terminal fragment of mVenus for use in bimolecular
                     fluorescence complementation (BiFC) (Kodama and Hu, 2010)"
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     promoter        complement(5313..5331)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(join(7992..7993,1..17))
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc
       61 gttctaacga caatatgtcc atatggtgca ctctcagtac aatctgctct gatgccgcat
      121 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
      181 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
      241 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
      301 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
      361 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      421 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
      481 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
      541 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
      601 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
      661 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
      721 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
      781 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
      841 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
      901 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
      961 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     1021 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     1081 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     1141 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     1201 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     1261 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     1321 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     1381 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     1441 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1501 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1561 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     1621 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     1681 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     1741 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     1801 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     1861 acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga
     1921 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     1981 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2041 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2101 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     2161 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     2221 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     2281 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg caggttaacc tggcttatcg
     2341 aaattaatac gactcactat agggagaccg gcagatccaa tgcggatgga ttcgctcact
     2401 cggtgccgcg gcggtgcgaa gttcctgccg tggattgggg gattgcacag acggtggagt
     2461 gcttgctaca gctaggtacc cagttgcaca ccaaacggtg cccctgtgcc cctgcgtcta
     2521 ggtgtgtcct gtgagcaact gcgcccgcgc gcggcaccgg gaagctgcgg gaacctcaat
     2581 ggatgattgg cgccagatcc ccggcatcac ctcagcacat gggcagcccg agaaggaatc
     2641 gagggtagaa gatattccag ggtttccgct agcacaactt ccaagcgcct ctaccgctac
     2701 tccacacacc cacgctgaat ggcacaacca ctgtaggcct agcggtccca gctccccgta
     2761 accacgcagc cccagcgacc gaggtcatca tgacactatg acgacaatct ggcgggagac
     2821 ttgggaaaat cgatgacggg cacctacctt gggaacaata gagcaagtcc agtcaacgcc
     2881 ctagggaagg gggaaaaaaa aaaagagccc attctcacaa ggagcaacac tgtgctgcta
     2941 tcatagtaca ctacaagcaa agtcgggatg ttgctagaat ccgtcggcta cgtcggtaga
     3001 tacctcccat aggcagtaag tctaggtatc caatttatag ttccgcgtat ttcttcgtcg
     3061 aacggggcac agcaaagcct aacagccgca gtgcctcgca accaacacca agcagtaatc
     3121 tcatccatcc atacagatcc tcccacaata tctttacatg cgcacttgcg ctgtgttagc
     3181 agtttaggtt ccactaccaa ggctgacacg agcagcactg acagcaaact gctgcttggg
     3241 gccaagaaat tggcagtttg ctgagcacct acatatcagt acacatcaac cctggtttaa
     3301 ttgttagcct ggtactgtta accgtcatga acgaggacgt ggaggaagaa ccacgctaac
     3361 aattgaacca gggttgatgt atacctctct taccatattt gcttgcttcc acgtccctat
     3421 cctcaagcga ttttgttctt gactttttgt atttcaacgc aaaggccatt caggtttctc
     3481 atgttcaacc ctttggatgg cgatgccgtt cgtcccagga cctcgtgacc tttgaaagac
     3541 gagaaatata tcccaaagca acttctgctt atggacaagc aactgcatgg cggcccacaa
     3601 ctcatatctc gcgtaatcat ccaaacgcta ggcccgagta ctctactgtt ctcgagacac
     3661 ctaccataat atgtacccaa tcagacatga caacagtcaa ccaaacatac cctgatccgg
     3721 ccaaaccctg acatccctag atgatggtta ggcacacaaa tggaaaacgg gactcgttgg
     3781 ttaggtggga acgcttgtat tgcagccagc caaccgactc tcctcgtccc tctcgagtcc
     3841 cgttattgcc gtttgaccca gccgcccagc cgccaaacag ctgagggagc caatggtcta
     3901 atacaccgtc caacggcccc agtgatgttt caaacttgat tgacagcgaa cgaaacccct
     3961 gaaacccgtt atagatatgt atagatttga cggggtagct tggcccttaa tacgactcac
     4021 tatagggcga attggagctc caccgcggtg gcggccgcta gaaggagcag tccatctgcg
     4081 tgaatcacga gagaatcagc tactttgaat cgatggatgc agctaccaga agtcactcag
     4141 ttcgttcaaa gccacatcac tgggcacttc cattgggaca ggcattgatc ggacgagacc
     4201 gacttctggc cgctttcaac agccacatta tatccatgtc acggctacgc gtcggccttc
     4261 ggtaacagaa aagcacacag acagcgattg tgacatggat tcgggcaaag cattggtggt
     4321 cgcaccaggt catcctgagt gtgcagtggc tgctattcag atttcatcta actgcgggag
     4381 aggggttcaa aggggcgtga cgtcacagac aacgggtgaa ggacgaagat tgcctcactt
     4441 ctttgctagc aatttgcctg caaagaaggc gcacatgaca agcaaacaac tgggaaacca
     4501 ctattgaata cccacaatgc aaagctcgga aggtacgtct tgattgcagt gtgtcgagtg
     4561 tcaaaaaaga agcaagtgtt catgcaagcc aaaattggca cctcctccac ttctccgagt
     4621 gccccacccg aacctccagg cgagatggcc ggaacatacc atccgcgttg ggattatgac
     4681 gtatctcctt cttcttcaca tgattccatc ccgttgttgc ttgtttgcga gctgtgacgg
     4741 gagatcgtag atgccacttc gggccaggca ggcagtgcag gcagccagga acacaagctt
     4801 ccaacttggt catctcgatt gccgattaag ggaaccaaat gcctatataa gactgtcctc
     4861 ccacctcccc aataccattc ttttcttctt ccatcatcag ccaacaaagc aatcacatct
     4921 tcactacttc aaatcaacac aacactcaaa ccactttcac aacccctcac atcaaccaaa
     4981 tctagaacta gtggatcccc gggttaatta acaaacagaa ggtcatgaac cacgacaagc
     5041 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     5101 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     5161 acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     5221 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     5281 acaagtaagg gcccggtacc cagcttttgt tccctttagt gagggttaat tagggcgtgg
     5341 acggctaatg gggtctgaat gctaaaagac accatttccc acactccctc tgattttttt
     5401 tatggttttt ttttatcttc atttttcgcc gtcttttatt cggttgtttg ggaggttcac
     5461 atgcaacgtg caagactgca agttttttga gatgggcgct tcatgcaatg cagcatcact
     5521 gcagggacat tgtgacatcc acgtttcgat cgtctgtgct tcccccgttc atgtacgcat
     5581 gatctgaaaa aaaaaaaaaa aaaaaaggtt gatgtaataa tgaaaataaa cacccaggga
     5641 cgagcacctc atctgctgga atagggagaa aaactcaagg gccgtttggg tctccacact
     5701 taccaacaga ttcatgtact gatcttcagc ctgtacagaa cagtacaatg tccaaaactc
     5761 gttatcatta ctctatgtgc ccaaaatgac ccagatatac atcaacccac tacatccttt
     5821 tgttcttgtt tttttttttt ttttttgcta cttcgaaaag aatttccgtt tagttgctct
     5881 tgctcatgtg ctcaagacgg atgctgaccg cccttctgtg agcctcgagc tcctcaacct
     5941 tagccaactg catgacagcc tggccgacgt ttttgagacc ctcggcagtc aagttggagc
     6001 tggtaatgtg cttgacgaac gaggcgaaat tgacgccaga gtactgcttg ccaaagccat
     6061 aggtagctat gtgtcttgac gttagcagaa gaatctgtga agcggggcta tttatgatat
     6121 gtaacttacg cagcgagtgg ttaacaccag cagagtaatc gccaacggac tcaggagtcc
     6181 aggcgccaat gaagacacta ccggcgttca tgacaagatc gacagccttc tcggcctcct
     6241 tgatctggag gatcaagtgc tcaggagcgt acttgttgct gagctccatg gcctcctcga
     6301 cggtcttgac ctgcacggtg atcgagtggg cgatggagcc acggacaatc tggacgcgag
     6361 gaagctccgt agcctgacgg tgaacctcgt cctcaatagc ctgaagatgc tcctcgtcga
     6421 ggtcaatagc gatcaggatg acctgactgt caacgccgtg ctcagcctgg gacaggagat
     6481 ccgaggcaac gaacgcgggg ttggcgtcct tgtcagcgat gaccagcacc tcggacgggc
     6541 cagcgggcat gtcaatacca acggcagcgt tggtgtcgtt gctgacgaac atcttggcag
     6601 cagtgacgaa ctggttaccg gggccgagaa tcttgtcgac cttggtgatg ctctcggtgc
     6661 cgtaggccat ggcagctacg gcctgggcac cgccggcaag cacgatggac tcggccccaa
     6721 ccttgtgagc gacgtggaca atctcgggag tgatggttcc gtcggcgcgg ggaggagagg
     6781 cgaacacaat cttgttgcag ccggcgacca tggcgggaac acccagcata agggcagtgc
     6841 tggggagaac ggcggtaccg ccggggatgt agcagccgac ggcctcgatg ggacgagaga
     6901 aacggctgca gacaacaccg ggcatggtct cgacctggag gggcttctcc tccttctggg
     6961 cggcgtggaa cttgcggatg ttctcgaagg acacgtcgat ggcagcaatg gtctcctcag
     7021 ggagctgcat aagctccttg gggaagggcg ccttcaggac ggggctagta agagaggtag
     7081 ccttctcgaa cttgtgagtg tacgacagaa cagccttgtc gccgttcttg cggacgtcct
     7141 cgatgatggg gacaatgatc ttgtagatgg catcggacga cttttgcgca ggacgcttga
     7201 gagcagcatc gagctcctcg gtagagacct tggaggcgtc gaaacgtctc atggtgatct
     7261 tctcaggggt ggtctcctgg gcggcctcct tggtgacagg ggccgaagtg gcagcgagag
     7321 cggacgccgc aggcttgatg ccctccttct cagcccactt acccttagca tctccagtcc
     7381 tgcgcttgac cttccagctc ttggcgtcaa ggctcctttc gatatcggca agagtaacgc
     7441 cggcagcaac ggccctggta agagcaaagt agaagagatc ggcagcctca aaggcgattt
     7501 cctggggggt ctgagcggtg cagagctcct cagcctcctc catgatcttg gcccggacta
     7561 gcttctcatc ggagaagaga cgggcagtgt aggagccctc gggggcagac tgtttcctcg
     7621 aaatcaaagt ctgctcgagc ttgggaaggc ctttgagctg accaaagcag ccggactgat
     7681 cgaggtggca gaaacgaccc ttctgcttca cgacaaactt gagagcatcg ttatcgcagt
     7741 caagcgagat gcggacgagc tcctgagtgt ctccggaagt agcacccttg taccagagac
     7801 cgcgcttccg gctctgatag acaccagtct gtgtcctgag ggcctcgttc acactctcgg
     7861 cactgctgta gaccagaccc agagcagtgt cgtgctcatc aacgacaacg gtggggagaa
     7921 gaccatcggg acggtcagac ttccagacgc tcgagaggat ggtggagatg gcaagcttca
     7981 gctgctcgag ttc
//
