LOCUS       V016441                 6241 bp    DNA     circular SYN 30-MAR-2020
DEFINITION  Exported.
ACCESSION   V016441
VERSION     V016441
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6241)
  AUTHORS   Sarkar S, Tack D, Ross D.
  TITLE     Mutual Information Landscapes as a Performance Metric for
            Biochemical Reaction Networks
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6241)
  AUTHORS   Tack D.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2020) Cellular Engineering, National Institute of
            Standards and Technology, 100 Bureau Dr MS8313, Gaithersburg, MD
            20878, USA
REFERENCE   3  (bases 1 to 6241)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (30-JAN-2020) Cellular Engineering, National Institute of Standards
            and Technology, 100 Bureau Dr MS8313, Gaithersburg, MD 20878, USA"
FEATURES             Location/Qualifiers
     source          1..6241
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..53
                     /label="L3S3P21"
                     /note="L3S3P21"
                     /regulatory_class="terminator"
     promoter        75..103
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    complement(109..128)
                     /label="lac operator (symmetric)"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The
                     symmetric lac operator was optimized for tight binding of
                     lac repressor."
     misc_RNA        130..180
                     /label="sTRSV HHRz"
                     /note="hammerhead ribozyme from the tobacco ringspot virus
                     satellite RNA (Khvorova et al., 2003)"
     regulatory      212..223
                     /regulatory_class="ribosome_binding_site"
     CDS             230..946
                     /label="EYFP"
                     /note="enhanced YFP"
     regulatory      988..994
                     /regulatory_class="ribosome_binding_site"
     CDS             1001..2251
                     /gene="lacY"
                     /label="Lactose permease"
                     /note="Lactose permease from Escherichia coli (strain K12).
                     Accession#: P02920"
     regulatory      2255..2315
                     /label="L3S2P21"
                     /note="L3S2P21"
                     /regulatory_class="terminator"
     regulatory      2320..2373
                     /label="PLacI"
                     /note="PLacI"
                     /regulatory_class="promoter"
     CDS             2402..3481
                     /label="lacI"
                     /note="lac repressor"
     CDS             3503..4123
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     regulatory      4130..4246
                     /label="araC terminator"
                     /note="araC terminator"
                     /regulatory_class="terminator"
     rep_origin      complement(4693..5237)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(5329..6141)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 ccaattattg aaggcctccc taacgggggg cctttttttg tttctggtct cccgcttaac
       61 gatcgttggc tgtgttgaca attaatcatc ggctcgtata atgtgtggaa ttgtgagcgc
      121 tcacaattag ctgtcaccgg atgtgctttc cggtctgatg agtccgtgag gacgaaacag
      181 cctctacaaa taattttgtt taatactaga gaaagagggg aaatactaga tggtgagcaa
      241 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
      301 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
      361 cctgaagttc atctgcacca caggcaagct gcccgtgccc tggcccaccc tcgtgaccac
      421 cttcggctac ggcctgcaat gcttcgcccg ctaccccgac cacatgaagc tgcacgactt
      481 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
      541 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
      601 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
      661 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
      721 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
      781 gcagaacacc ccaatcggcg acggccccgt gctgctgccc gacaaccact accttagcta
      841 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
      901 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaat aataaccggg
      961 caggccatgt ctgcccgtat ttcgcgtaag gaaatccatt atgtactatt taaaaaacac
     1021 aaacttttgg atgttcggtt tattcttttt cttttacttt tttatcatgg gagcctactt
     1081 cccgtttttc ccgatttggc tacatgacat caaccatatc agcaaaagtg atacgggtat
     1141 tatttttgcc gctatttctc tgttctcgct attattccaa ccgctgtttg gtctgctttc
     1201 tgacaaactc gggctgcgca aatacctgct gtggattatt accggcatgt tagtgatgtt
     1261 tgcgccgttc tttattttta tcttcgggcc actgttacaa tacaacattt tagtaggatc
     1321 gattgttggt ggtatttatc taggcttttg ttttaacgcc ggtgcgccag cagtagaggc
     1381 atttattgag aaagtcagcc gtcgcagtaa tttcgaattt ggtcgcgcgc ggatgtttgg
     1441 ctgtgttggc tgggcgctgt gtgcctcgat tgtcggcatc atgttcacca tcaataatca
     1501 gtttgttttc tggctgggct ctggctgtgc actcatcctc gccgttttac tctttttcgc
     1561 caaaacggat gcgccctctt ctgccacggt tgccaatgcg gtaggtgcca accattcggc
     1621 atttagcctt aagctggcac tggaactgtt cagacagcca aaactgtggt ttttgtcact
     1681 gtatgttatt ggcgtttcct gcacctacga tgtttttgac caacagtttg ctaatttctt
     1741 tacttcgttc tttgctaccg gtgaacaggg tacgcgggta tttggctacg taacgacaat
     1801 gggcgaatta cttaacgcct cgattatgtt ctttgcgcca ctgatcatta atcgcatcgg
     1861 tgggaaaaac gccctgctgc tggctggcac tattatgtct gtacgtatta ttggctcatc
     1921 gttcgccacc tcagcgctgg aagtggttat tctgaaaacg ctgcatatgt ttgaagtacc
     1981 gttcctgctg gtgggctgct ttaaatatat taccagccag tttgaagtgc gtttttcagc
     2041 gacgatttat ctggtctgtt tctgcttctt taagcaactg gcgatgattt ttatgtctgt
     2101 actggcgggc aatatgtatg aaagcatcgg tttccagggc gcttatctgg tgctgggtct
     2161 ggtggcgctg ggcttcacct taatttccgt gttcacgctt agcggccccg gcccgctttc
     2221 cctgctgcgt cgtcaggtga atgaagtcgc ttaactcggt accaaattcc agaaaagagg
     2281 cctcccgaaa ggggggcctt ttttcgtttt ggtccaatgg cggcgcgcca tcgaatggcg
     2341 caaaaccttt cgcggtatgg catgatagcg cccggaagag agtcaattca gggtggtgaa
     2401 tatgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt
     2461 ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc
     2521 ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca
     2581 gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt
     2641 cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga
     2701 acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag
     2761 tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg
     2821 cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat
     2881 tttctcccat gaggacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca
     2941 gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg
     3001 ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg
     3061 gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac
     3121 tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc
     3181 cgggctgcgc gttggtgcgg atatctcggt agtgggatac gacgataccg aagatagctc
     3241 atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag
     3301 cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc
     3361 agtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg
     3421 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     3481 gtgataatcc aggaggaaaa aaatgtccag attagataaa agtaaagtga ttaacagcgc
     3541 attagagctg cttaatgagg tcggaatcga aggtttaaca acccgtaaac tcgcccagaa
     3601 gctaggtgta gagcagccta cattgtattg gcatgtaaaa aataagcggg ctttgctcga
     3661 cgccttagcc attgagatgt tagataggca ccatactcac ttttgccctt tagaagggga
     3721 aagctggcaa gattttttac gtaataacgc taaaagtttt agatgtgctt tactaagtca
     3781 tcgcgatgga gcaaaagtac atttaggtac acggcctaca gaaaaacagt atgaaactct
     3841 cgaaaatcaa ttagcctttt tatgccaaca aggtttttca ctagagaatg cattatatgc
     3901 actcagcgct gtggggcatt ttactttagg ttgcgtattg gaagatcaag agcatcaagt
     3961 cgctaaagaa gaaagggaaa cacctactac tgatagtatg ccgccattat tacgacaagc
     4021 tatcgaatta tttgatcacc aaggtgcaga gccagccttc ttattcggcc ttgaattgat
     4081 catatgcgga ttagaaaaac aacttaaatg tgaaagtggg tcctaataat tggtaacgaa
     4141 tcagacaatt gacggctcga gggagtagca tagggtttgc agaatccctg cttcgtccat
     4201 ttgacaggca cattatgcat cgatgataag ctgtcaaaca tgagcagatc ctctacgccg
     4261 gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg
     4321 acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcaaa tattttatct
     4381 gaggtgcttc ctcgctcact gactcgctgc acgaggcaga cctcagcgct agcggagtgt
     4441 atactggctt actatgttgg cactgatgag ggtgtcagtg aagtgcttca tgtggcagga
     4501 gaaaaaaggc tgcaccggtg cgtcagcaga atatgtgata caggatatat tccgcttcct
     4561 cgctcactga ctcgctacgc tcggtcgttc gactgcggcg agcggaaatg gcttacgaac
     4621 ggggcggaga tttcctggaa gatgccagga agatacttaa cagggaagtg agagggccgc
     4681 ggcaaagccg tttttccata ggctccgccc ccctgacaag catcacgaaa tctgacgctc
     4741 aaatcagtgg tggcgaaacc cgacaggact ataaagatac caggcgtttc ccctggcggc
     4801 tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg
     4861 ccgcgtttgt ctcattccac gcctgacact cagttccggg taggcagttc gctccaagct
     4921 ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttatccg gtaactatcg
     4981 tcttgagtcc aacccggaaa gacatgcaaa agcaccactg gcagcagcca ctggtaattg
     5041 atttagagga gttagtcttg aagtcatgcg ccggttaagg ctaaactgaa aggacaagtt
     5101 ttggtgactg cgctcctcca agccagttac ctcggttcaa agagttggta gctcagagaa
     5161 ccttcgaaaa accgccctgc aaggcggttt tttcgttttc agagcaagag attacgcgca
     5221 gaccaaaacg atctcaagaa gatcatctta ttaaggggtc tgacgctcag tggaacgaaa
     5281 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccttaga aaaactcatc
     5341 gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa
     5401 aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc
     5461 ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc
     5521 gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa
     5581 tggcaaaagc ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc
     5641 atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgagacg
     5701 aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag
     5761 gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg
     5821 gaatgctgtt ttcccgggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat
     5881 aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc
     5941 atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc
     6001 gggcttccca tacaatcgat agattgtcgc acctgattgc ccgacattat cgcgagccca
     6061 tttataccca tataaatcag catccatgtt ggaatttaat cgcggcctcg agcaagacgt
     6121 ttcccgttga atatggctca taacacccct tgtattactg tttatgtaag cagacagttt
     6181 tattgttcat gatgatatat ttttatcttg tgcaatgtac atcagagatt ttgagacaca
     6241 a
//
