LOCUS       40924_48083        6170 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pYEUra3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6170)
  AUTHORS   Kitts PA.
  TITLE     CLONTECH Vectors On Disc version 1.3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6170)
  AUTHORS   Kitts PA.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-OCT-1993) Paul A. Kitts, CLONTECH Laboratories, Inc., 
            1020 East Meadow Circle, Palo Alto, CA 94303, USA
REFERENCE   3  (bases 1 to 6170)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6170)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-OCT-1993) Paul A. Kitts, CLONTECH Laboratories, Inc., 1020 East 
            Meadow Circle, Palo Alto, CA 94303, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     This vector can be obtained from CLONTECH Laboratories, Inc., 1020 
            East Meadow Circle, Palo Alto, CA 94303, USA. To place an order call
            (415) 424-8222 or (800) 662-2566, extension 1. International 
            customers, please contact your local distributor.  For technical 
            information, call (415) 424- 8222 or (800) 662-2566, extension 3. 
            This sequence has been compiled from information in the sequence 
            databases, published literature and other sources, together with 
            partial sequences obtained by CLONTECH; this vector has not been 
            completely sequenced. If you suspect there is an error in this 
            sequence, please contact CLONTECH's Technical Service Department at 
            (415) 424-8222 or (800) 662-2566, extension 3 or E-mail 
            TECH@CLONTECH.COM.
FEATURES             Location/Qualifiers
     source          1..6170
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     472..488
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        495..513
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(594..1258)
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
     CDS             complement(1504..2304)
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(2305..2520)
                     /label=URA3 promoter
     rep_origin      2522..2975
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence
                     ARS1"
     misc_feature    2976..3268
                     /label=CEN4
                     /note="S. cerevisiae CEN4 centromere"
     misc_feature    3969..4109
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4295..4883)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5057..5914)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5915..6019)
                     /label=AmpR promoter
     rep_origin      6046..6170
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
       61 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
      121 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
      181 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
      241 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
      301 aattttaaca aaatattaac gcttacaatt tccattcgcc attcaggctg cgcaactgtt
      361 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
      421 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
      481 cggccagtga attgtaatac gactcactat agggcgaatt gggtaccggg ccccccctcg
      541 aggtcgactc tagaggatcc ccgggtaccg agctcgaatt aattcggcta gaggtttttt
      601 ctccttgacg ttaaagtata gaggtatatt aacaattttt tgttgatact tttattacat
      661 ttgaataaga agtaatacaa accgaaaatg ttgaaagtat tagttaaagt ggttatgcag
      721 tttttgcatt tatatatctg ttaatagatc aaaaatcatc gcttcgctga ttaattaccc
      781 cagaaataag gctaaaaaac taatcgcatt atcatcctat ggttgttaat ttgattcgtt
      841 catttgaagg tttgtggggc caggttactg ccaatttttc ctcttcataa ccataaaagc
      901 tagtattgta gaatctttat tgttcggagc agtgcggcgc gaggcacatc tgcgtttcag
      961 gaacgcgacc ggtgaagacg aggacgcacg gaggagagtc ttccttcgga gggctgtcac
     1021 ccgctcggcg gcttctaatc cgtacttcaa tatagcaatg agcagttaag cgtattactg
     1081 aaagttccaa agagaaggtt tttttaggct aagataatgg ggctctttac atttccacaa
     1141 catataagta agattagata tggatatgta tatggatatg tatatggtgg taatgccatg
     1201 taatatgatt attaaacttc tttgcgtcca tccaaaaaaa aagtaagaat ttttgaaaat
     1261 tcaatataaa tgacagctca gttacaaagt gaaagtactt ctaaaattgt tttggttaca
     1321 ggtggtgctg gatacattgg ttcacacact gtggtagagc taattgagaa tggatatgac
     1381 tgtgttgttg ctgataacct gtcgaattcg agctcggtac ccgggtaata actgatataa
     1441 ttaaattgaa gctctaattt gtgagtttag tatacatgca tttacttata atacagtttt
     1501 ttagttttgc tggccgcatc ttctcaaata tgcttcccag cctgcttttc tgtaacgttc
     1561 accctctacc ttagcatccc ttccctttgc aaatagtcct cttccaacaa taataatgtc
     1621 agatcctgta gagaccacat catccacggt tctatactgt tgacccaatg cgtctccctt
     1681 gtcatctaaa cccacaccgg gtgtcataat caaccaatcg taaccttcat ctcttccacc
     1741 catgtctctt tgagcaataa agccgataac aaaatctttg tcgctcttcg caatgtcaac
     1801 agtaccctta gtatattctc cagtagatag ggagcccttg catgacaatt ctgctaacat
     1861 caaaaggcct ctaggttcct ttgttacttc ttctgccgcc tgcttcaaac cgctaacaat
     1921 acctgggccc accacaccgt gtgcattcgt aatgtctgcc cattctgcta ttctgtatac
     1981 acccgcagag tactgcaatt tgactgtatt accaatgtca gcaaattttc tgtcttcgaa
     2041 gagtaaaaaa ttgtacttgg cggataatgc ctttagcggc ttaactgtgc cctccatgga
     2101 aaaatcagtc aagatatcca catgtgtttt tagtaaacaa attttgggac ctaatgcttc
     2161 aactaactcc agtaattcct tggtggtacg aacatccaat gaagcacaca agtttgtttg
     2221 cttttcgtgc atgatattaa atagcttggc agcaacagga ctaggatgag tagcagcacg
     2281 ttccttatat gtagctttcg acatgattta tcttcgtttc ctgcaggttt ttgttctgtg
     2341 cagttgggtt aagaatactg ggcaatttca tgtttcttca acactacata tgcgtatata
     2401 taccaatcta agtctgtgct ccttccttcg ttcttccttc tgttcggaga ttaccgaatc
     2461 aaaaaaattt caaggaaacc gaaatcaaaa aaaagaataa aaaaaaaatg atgaattgaa
     2521 aagcttacat tttatgttag ctggtggact gacgccagaa aatgttggtg atgcgcttag
     2581 attaaatggc gttattggtg ttgatgtaag cggaggtgtg gagacaaatg gtgtaaaaga
     2641 ctctaacaaa atagcaaatt tcgtcaaaaa tgctaagaaa taggttatta ctgagtagta
     2701 tttatttaag tattgtttgt gcacttgcct gcaggccttt tgaaaagcaa gcataaaaga
     2761 tctaaacata aaatctgtaa aataacaaga tgtaaagata atgctaaatc atttggcttt
     2821 ttgattgatt gtacaggaaa atatacatcg cagggggttg acttttacca tttcaccgca
     2881 atggaatcaa acttgttgaa gagaatgttc acaggcgcat acgctacaat gacccgattc
     2941 ttgctagcct tttctcggtc ttgcaaacaa ccgccaactc taagaggtga tacttattta
     3001 ctgtaaaact gtgacgataa aaccggaagg aagaataaga aaactcgaac tgatctataa
     3061 tgcctatttt ctgtaaagag tttaagctat gaaagcctcg gcattttggc cgctcctagg
     3121 tagtgctttt tttccaagga caaaacagtt tctttttctt gagcaggttt tatgtttcgg
     3181 taatcataaa caataaataa attatttcat ttatgtttaa aaataaaaaa taaaaaagta
     3241 ttttaaattt ttaaaaaagt tgattataag catgtgacct tttgcaagca attaaatttt
     3301 gcaatttgtg attttaggca aaagttacaa tttctggctc gtgtaatata tgtatgctaa
     3361 agtgaacttt tacaaagtcg atatggactt agtcaaaaga aattttctta aaaatatata
     3421 gcactagcca atttagcact tctttatgag atatattata gactttatta agccagattt
     3481 gtgtattata tgtatttacc cggcgaatca tggacataca ttctgaaata ggtaatattc
     3541 tctatggtga gacagcatag ataacctagg atacaagtta aaagctagta ctgttttgca
     3601 gtaatttttt tcttttttat aagaatgtta ccacctaaat aagttataaa gtcaatagtt
     3661 aagtttgata tttgattgta aaataccgta atatatttgc atgatcaaaa ggctcaatgt
     3721 tgactagcca gcatgtcaac cactatattg atcaccgata tatggacttc cacaccaact
     3781 agtaatatga caataaattc aagatattct tcatgagaat ggcccagctg cctcgcgcgt
     3841 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     3901 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     3961 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact
     4021 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     4081 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc
     4141 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     4201 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     4261 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     4321 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     4381 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     4441 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     4501 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     4561 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     4621 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     4681 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     4741 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     4801 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     4861 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     4921 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     4981 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     5041 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     5101 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     5161 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     5221 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     5281 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     5341 atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg
     5401 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     5461 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     5521 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     5581 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     5641 ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa
     5701 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     5761 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     5821 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     5881 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     5941 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     6001 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg
     6061 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     6121 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
//
