LOCUS       V001896                 7805 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V001896
VERSION     V001896
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7805)
  AUTHORS   Ward AC, Castelli LA, Macreadie IG, Azad AA.
  TITLE     Vectors for Cu(2+)-inducible production of glutathione
            S-transferase-fusion proteins for single-step purification from
            yeast
  JOURNAL   Yeast 10 (4), 441-449 (1994)
   PUBMED   7941730
REFERENCE   2  (bases 1 to 7805)
  AUTHORS   Ward AC.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUN-1993) Alister C. Ward, Medicine, University of
            Melbourne, Royal Melbourne Hospital, Parkville, Vic 3050, Australia
REFERENCE   3  (bases 1 to 7805)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7805)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast";
            date: "1994"; volume: "10"; issue: "4"; pages: "441-449"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (20-JUN-1993) Alister C. Ward, Medicine, University of Melbourne,
            Royal Melbourne Hospital, Parkville, Vic 3050, Australia"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7805
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(4..1350)
                     /direction=LEFT
                     /label="2u ori"
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(1464..2264)
                     /label="URA3"
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(2265..2480)
                     /label="URA3 promoter"
     promoter        2596..2700
                     /label="AmpR promoter"
     CDS             2701..3558
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      3732..4320
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    4608..4629
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4644..4674
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    4682..4698
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4706..4722
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             5024..5671
                     /label="GST"
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
     CDS             5678..5695
                     /label="thrombin site"
                     /note="thrombin recognition and cleavage site"
     CDS             5700..5873
                     /gene="CUP1-1"
                     /label="Copper metallothionein 1-1"
                     /note="Copper metallothionein 1-1 from Saccharomyces
                     cerevisiae (strain ATCC 204508 / S288c). Accession#:
                     P0CX80"
     CDS             complement(6344..7435)
                     /label="LEU2"
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
ORIGIN
        1 gttaacgaag catctgtgct tcattttgta gaacaaaaat gcaacgcgag agcgctaatt
       61 tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc gaaagcgcta
      121 ttttaccaac gaagaatctg tgcttcattt ttgtaaaaca aaaatgcaac gcgagagcgc
      181 taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgagag
      241 cgctatttta ccaacaaaga atctatactt cttttttgtt ctacaaaaat gcatcccgag
      301 agcgctattt ttctaacaaa gcatcttaga ttactttttt tctcctttgt gcgctctata
      361 atgcagtctc ttgataactt tttgcactgt aggtccgtta aggttagaag aaggctactt
      421 tggtgtctat tttctcttcc ataaaaaaag cctgactcca cttcccgcgt ttactgatta
      481 ctagcgaagc tgcgggtgca ttttttcaag ataaaggcat ccccgattat attctatacc
      541 gatgtggatt gcgcatactt tgtgaacaga aagtgatagc gttgatgatt cttcattggt
      601 cagaaaatta tgaacggttt cttctatttt gtctctatat actacgtata ggaaatgttt
      661 acattttcgt attgttttcg attcactcta tgaatagttc ttactacaat ttttttgtct
      721 aaagagtaat actagagata aacataaaaa atgtagaggt cgagtttaga tgcaagttca
      781 aggagcgaaa ggtggatggg taggttatat agggatatag cacagagata tatagcaaag
      841 agatactttt gagcaatgtt tgtggaagcg gtattcgcaa tattttagta gctcgttaca
      901 gtccggtgcg tttttggttt tttgaaagtg cgtcttcaga gcgcttttgg ttttcaaaag
      961 cgctctgaag ttcctatact ttctagctag agaataggaa cttcggaata ggaacttcaa
     1021 agcgtttccg aaaacgagcg cttccgaaaa tgcaacgcga gctgcgcaca tacagctcac
     1081 tgttcacgtc gcacctatat ctgcgtgttg cctgtatata tatatacatg agaagaacgg
     1141 catagtgcgt gtttatgctt aaatgcgtac ttatatgcgt ctatttatgt aggatgaaag
     1201 gtagtctagt acctcctgtg atattatccc attccatgcg gggtatcgta tgcttccttc
     1261 agcactaccc tttagctgtt ctatatgctg ccactcctca attggattag tctcatcctt
     1321 caatgctatc atttcctttg atattggatc gatccgatga taagctgtca aacatgagaa
     1381 ttgggtaata actgatataa ttaaattgaa gctctaattt gtgagtttag tatacatgca
     1441 tttacttata atacagtttt ttagttttgc tggccgcatc ttctcaaata tgcttcccag
     1501 cctgcttttc tgtaacgttc accctctacc ttagcatccc ttccctttgc aaatagtcct
     1561 cttccaacaa taataatgtc agatcctgta gagaccacat catccacggt tctatactgt
     1621 tgacccaatg cgtctccctt gtcatctaaa cccacaccgg gtgtcataat caaccaatcg
     1681 taaccttcat ctcttccacc catgtctctt tgagcaataa agccgataac aaaatctttg
     1741 tcgctcttcg caatgtcaac agtaccctta gtatattctc cagtagatag ggagcccttg
     1801 catgacaatt ctgctaacat caaaaggcct ctaggttcct ttgttacttc ttctgccgcc
     1861 tgcttcaaac cgctaacaat acctgggccc accacaccgt gtgcattcgt aatgtctgcc
     1921 cattctgcta ttctgtatac acccgcagag tactgcaatt tgactgtatt accaatgtca
     1981 gcaaattttc tgtcttcgaa gagtaaaaaa ttgtacttgg cggataatgc ctttagcggc
     2041 ttaactgtgc cctccatgga aaaatcagtc aagatatcca catgtgtttt tagtaaacaa
     2101 attttgggac ctaatgcttc aactaactcc agtaattcct tggtggtacg aacatccaat
     2161 gaagcacaca agtttgtttg cttttcgtgc atgatattaa atagcttggc agcaacagga
     2221 ctaggatgag tagcagcacg ttccttatat gtagctttcg acatgattta tcttcgtttc
     2281 ctgcatgttt ttgttctgtg cagttgggtt aagaatactg ggcaatttca tgtttcttca
     2341 acactacata tgcgtatata taccaatcta agtctgtgct ccttccttcg ttcttccttc
     2401 tgttcggaga ttaccgaatc aaaaaaattt caaagaaacc gaaatcaaaa aaaagaataa
     2461 aaaaaaaatg atgaattgaa ttgaaaagct aattcttgaa gacgaaaggg cctcgtgata
     2521 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     2581 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     2641 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     2701 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     2761 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     2821 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     2881 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     2941 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     3001 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     3061 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     3121 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     3181 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     3241 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     3301 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     3361 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     3421 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     3481 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     3541 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     3601 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     3661 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     3721 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     3781 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     3841 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     3901 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     3961 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     4021 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     4081 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     4141 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     4201 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     4261 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     4321 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     4381 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     4441 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     4501 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     4561 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     4621 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     4681 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctt
     4741 gcatgtcttt tgctggcatt tcttctagaa gcaaaaagag cgatgcgtct tttccgctga
     4801 accgttccag caaaaaagac taccaacgca atatggattg tcagaatcat ataaaagaga
     4861 agcaaataac tccttgtctt gtatcaattg cattataata tcttcttgtt agtgcaatat
     4921 catatagaag tcatcgaaat agatattaag aaaaacaaac tgtacaatca atcaatcaat
     4981 catcacataa aatattcagc gaattggatc tatgaccaag ttacctatac taggttattg
     5041 gaaaattaag ggccttgtgc aacccactcg acttcttttg gaatatcttg aagaaaaata
     5101 tgaagagcat ttgtatgagc gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt
     5161 gggtttggag tttcccaatc ttccttatta tattgatggt gatgttaaat taacacagtc
     5221 tatggccatc atacgttata tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga
     5281 gcgtgcagag atttcaatgc ttgaaggagc ggttttggat attagatacg gtgtttcgag
     5341 aattgcatat agtaaagact ttgaaactct caaagttgat tttcttagca agctacctga
     5401 aatgctgaaa atgttcgaag atcgtttatg tcataaaaca tatttaaatg gtgatcatgt
     5461 aacccatcct gacttcatgt tgtatgacgc tcttgatgtt gttttataca tggacccaat
     5521 gtgcctggat gcgttcccaa aattagtttg ttttaaaaaa cgtattgaag ctatcccaca
     5581 aattgataag tacttgaaat ccagcaagta tatagcatgg cctttgcagg gctggcaagc
     5641 cacgtttggt ggtggcgacc atcctccaaa atcggatctg gttccgcgtg gatccccggg
     5701 aattcattaa cttccaaaat gaaggtcatg agtgccaatg ccaatgtggt agctgcaaaa
     5761 ataatgaaca atgccaaaaa tcatgtagct gcccaacggg gtgtaacagc gacgacaaat
     5821 gcccctgcgg taacaagtct gaagaaacca agaagtcatg ctgctctggg aaatgaaacg
     5881 aatagtcttt aatatattca tctaactatt tgctgttttt aatttttaaa aggagaagga
     5941 agtttaatcg acgattctac tcagtttgag tacacttatg tattttgttt agatactttg
     6001 ttaatttata ggtatacgtt aataattaag aaaaggaaat aaagtatctc catatgtcgc
     6061 cccaagaata aaatattatt accaaattct agtttgccta acttagaact ctgtatagaa
     6121 tccccagatt tcgaataaaa aaaaaaaaaa agctattcat ggtaccgcga tgtagtaaaa
     6181 ctagctagac cgagaaagag actagaaatg caaaaggcac ttctacaatg gctgccatca
     6241 ttattatccg atgtgacgct gcattttttt tttttttttt tttttttttt tttttttttt
     6301 tttttttttt gtacaaatat cataaaaaaa gagaatcttt ttaagcaagg attttcttaa
     6361 cttcttcggc gacagcatca ccgacttcgg tggtactgtt ggaaccacct aaatcaccag
     6421 ttctgatacc tgcatccaaa acctttttaa ctgcatcttc aatggcctta ccttcttcag
     6481 gcaagttcaa tgacaatttc aacatcattg cagcagacaa gatagtggcg atagggtcaa
     6541 ccttattctt tggcaaatct ggagcagaac cgtggcatgg ttcgtacaaa ccaaatgcgg
     6601 tgttcttgtc tggcaaagag gccaaggacg cagatggcaa caaacccaag gaacctggga
     6661 taacggaggc ttcatcggag atgatatcac caaacatgtt gctggtgatt ataataccat
     6721 ttaggtgggt tgggttctta actaggatca tggcggcaga atcaatcaat tgatgttgaa
     6781 ccttcaatgt aggaaattcg ttcttgatgg tttcctccac agtttttctc cataatcttg
     6841 aagaggccaa aacattagct ttatccaagg accaaatagg caatggtggc tcatgttgta
     6901 gggccatgaa agcggccatt cttgtgattc tttgcacttc tggaacggtg tattgttcac
     6961 tatcccaagc gacaccatca ccatcgtctt cctttctctt accaaagtaa atacctccca
     7021 ctaattctct gacaacaacg aagtcagtac ctttagcaaa ttgtggcttg attggagata
     7081 agtctaaaag agagtcggat gcaaagttac atggtcttaa gttggcgtac aattgaagtt
     7141 ctttacggat ttttagtaaa ccttgttcag gtctaacact acctgtaccc catttaggac
     7201 cacccacagc acctaacaaa acggcatcaa ccttcttgga ggcttccagc gcctcatctg
     7261 gaagtgggac acctgtagca tcgatagcag caccaccaat taaatgattt tcgaaatcga
     7321 acttgacatt ggaacgaaca tcagaaatag ctttaagaac cttaatggct tcggctgtga
     7381 tttcttgacc aacgtggtca cctggcaaaa cgacgatctt cttaggggca gacattacaa
     7441 tggtatatcc ttgaaatata tataaaaaaa aaaaaaaaaa aaaaaaaaaa aaaatgcagc
     7501 ttctcaatga tattcgaata cgctttgagg agatacagcc taatatccga caaactgttt
     7561 tacagattta cgatcgtact tgttacccat cattgaattt tgaacatccg aacctgggag
     7621 ttttccctga aacagatagt atatttgaac ctgtataata atatatagtc tagcgcttta
     7681 cggaagacaa tgtatgtatt tcggttcctg gagaaactat tgcatctatt gcataggtaa
     7741 tcttgcacgt cgcatccccg gttcattttc tgcgtttcca tcttgcactt caatagcata
     7801 tcttt
//
