LOCUS       pYES6_CT.        5840 bp DNA     circular SYN 01-JAN-1980
DEFINITION  High-copy episomal vector with a blasticidin resistance marker for 
            galactose-inducible expression of C-terminally tagged proteins in S.
            cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    pYES6 CT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5840)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5840)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5840
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..443
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     promoter        475..493
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    501..600
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             607..648
                     /label=V5 tag
                     /note="epitope tag from simian virus 5"
     CDS             658..675
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      709..956
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(1204..1792)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1966..2823)
                     /label=AmpR
                     /note="beta-lactamase"
     CDS             complement(2946..3341)
                     /label=BSD
                     /note="blasticidin S deaminase"
     promoter        complement(3360..3407)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     promoter        complement(3414..3821)
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     rep_origin      complement(4419..5299)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     rep_origin      complement(5310..5823)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 acggattaga agccgccgag cgggtgacag ccctccgaag gaagactctc ctccgtgcgt
       61 cctcgtcttc accggtcgcg ttcctgaaac gcagatgtgc ctcgcgccgc actgctccga
      121 acaataaaga ttctacaata ctagctttta tggttatgaa gaggaaaaat tggcagtaac
      181 ctggccccac aaaccttcaa atgaacgaat caaattaaca accataggat gataatgcga
      241 ttagtttttt agccttattt ctggggtaat taatcagcga agcgatgatt tttgatctat
      301 taacagatat ataaatgcaa aaactgcata accactttaa ctaatacttt caacattttc
      361 ggtttgtatt acttcttatt caaatgtaat aaaagtatca acaaaaaatt gttaatatac
      421 ctctatactt taacgtcaag gagaaaaaac cccggatcgg actactagca gctgtaatac
      481 gactcactat agggaatatt aagcttggta ccgagctcgg atccactagt aacggccgcc
      541 agtgtgctgg aattctgcag atatccagca cagtggcggc cgctcgagtc tagagggccc
      601 ttcgaaggta agcctatccc taaccctctc ctcggtctcg attctacgcg taccggtcat
      661 catcaccatc accattgagt ttaaacccgc tgatcctaga gggccgcatc atgtaattag
      721 ttatgtcacg cttacattca cgccctcccc ccacatccgc tctaaccgaa aaggaaggag
      781 ttagacaacc tgaagtctag gtccctattt atttttttat agttatgtta gtattaagaa
      841 cgttatttat atttcaaatt tttctttttt ttctgtacag acgcgtgtac gcatgtaaca
      901 ttatactgaa aaccttgctt gagaaggttt tgggacgctc gaaggcttta atttgcaagc
      961 tgcggccctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     1021 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     1081 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     1141 gaacatgtga gcaaaaggcc agcaaaagcc caggaaccgt aaaaaggccg cgttgctggc
     1201 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     1261 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     1321 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     1381 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     1441 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     1501 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     1561 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     1621 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     1681 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     1741 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     1801 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     1861 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     1921 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     1981 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     2041 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     2101 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     2161 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     2221 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     2281 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     2341 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     2401 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     2461 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     2521 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     2581 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     2641 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     2701 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     2761 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     2821 catactcttc ctttttcaat gggtaataac tgatataatt aaattgaagc tctaatttgt
     2881 gagtttagta gacatgcaga tctcccggga cgtgtcagtc ctgctcctcg gccacgaagt
     2941 gcttagccct cccacacata accagagggc agcaattcac gaatcccaac tgccgtcggc
     3001 tgtccatcac tgtccttcac tatggctttg atcccaggat gcagatcgag aagaacctgt
     3061 cggcaccgtc cgcaggggct caagatgccc ctgttctcat ttccgatcgc gacgatacaa
     3121 gtcaggttgc cagctgccgc agcagcagca gtgcccagca ccacgagttc tgcgcaaggt
     3181 cccccagtaa aatgatatac attgacacca gtgaagatgc ggccgtcgct agagagagct
     3241 gcgctggcga cgctgtagtc ttcagagatg gggatgctgt tgattgtagc cgttgctctt
     3301 tcaatgaggg tggattcttc ttgagacaaa ggcttggcca tggtttagtt cctcaccttg
     3361 tcgtattata ctatgccgat atactatgcc gatgattaat tgtcaacacc gcccttagat
     3421 tagattgcta tgctttcttt ctaatgagca agaagtaaaa aaagttgtaa tagaacaaga
     3481 aaaatgaaac tgaaacttga gaaattgaag accgtttatt aacttaaata tcaatgggag
     3541 gtcatcgaaa gagaaaaaaa tcaaaaaaaa aaattttcaa gaaaaagaaa cgtgataaaa
     3601 atttttattg cctttttcga cgaagaaaaa gaaacgaggc ggtctctttt ttcttttcca
     3661 aacctttagt acgggtaatt aacgacaccc tagaggaaga aagaggggaa atttagtatg
     3721 ctgtgcttgg gtgttttgaa gtggtacggc gatgcgcgga gtccgagaaa atctggaaga
     3781 gtaaaaaagg agtagaaaca ttttgaagct atggtgtgtg gggctagctt atcgatgata
     3841 agctgtcaaa gatgagaatt aattccacgg actatagact atactagata ctccgtctac
     3901 tgtacgatac acttccgctc aggtccttgt cctttaacga ggccttacca ctcttttgtt
     3961 actctattga tccagctcag caaaggcagt gtgatctaag attctatctt cgcgatgtag
     4021 taaaactagc tagaccgaga aagagactag aaatgcaaaa ggcacttcta caatggctgc
     4081 catcattatt atccgatgtg acgctgcagc ttctcaatga tattcgaata cgctttgagg
     4141 agatacagcc taatatccga caaactgttt tacagattta cgatcgtact tgttacccat
     4201 cattgaattt tgaacatccg aacctgggag ttttccctga aacagatagt atatttgaac
     4261 ctgtataata atatatagtc tagcgcttta cggaagacaa tgtatgtatt tcggttcctg
     4321 gagaaactat tgcatctatt gcataggtaa tcttgcacgt cgcatccccg gttcattttc
     4381 tgcgtttcca tcttgcactt caatagcata tctttgttaa cgaagcatct gtgcttcatt
     4441 ttgtagaaca aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca
     4501 tttttacaga acagaaatgc aacgcgaaag cgctatttta ccaacgaaga atctgtgctt
     4561 catttttgta aaacaaaaat gcaacgcgac gagagcgcta atttttcaaa caaagaatct
     4621 gagctgcatt tttacagaac agaaatgcaa cgcgagagcg ctattttacc aacaaagaat
     4681 ctatacttct tttttgttct acaaaaatgc atcccgagag cgctattttt ctaacaaagc
     4741 atcttagatt actttttttc tcctttgtgc gctctataat gcagtctctt gataactttt
     4801 tgcactgtag gtccgttaag gttagaagaa ggctactttg gtgtctattt tctcttccat
     4861 aaaaaaagcc tgactccact tcccgcgttt actgattact agcgaagctg cgggtgcatt
     4921 ttttcaagat aaaggcatcc ccgattatat tctataccga tgtggattgc gcatactttg
     4981 tgaacagaaa gtgatagcgt tgatgattct tcattggtca gaaaattatg aacggtttct
     5041 tctattttgt ctctatatac tacgtatagg aaatgtttac attttcgtat tgttttcgat
     5101 tcactctatg aatagttctt actacaattt ttttgtctaa agagtaatac tagagataaa
     5161 cataaaaaat gtagaggtcg agtttagatg caagttcaag gagcgaaagg tggatgggta
     5221 ggttatatag ggatatagca cagagatata tagcaaagag atacttttga gcaatgtttg
     5281 tggaagcggt attcgcaatg ggaagctcca ccccggttga taatcagaaa agccccaaaa
     5341 acaggaagat tgtataagca aatatttaaa ttgtaaacgt taatattttg ttaaaattcg
     5401 cgttaaattt ttgttaaatc agctcatttt ttaacgaata gcccgaaatc ggcaaaatcc
     5461 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tccaacaaga
     5521 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaagggtc tatcagggcg
     5581 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     5641 cagtaaatcg gaagggtaaa cggatgcccc catttagagc ttgacgggga aagccggcga
     5701 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg ggctagggcg gtgggaagtg
     5761 taggggtcac gctgggcgta accaccacac ccgccgcgct taatggggcg ctacagggcg
     5821 cgtggggatg atccactagt
//
