LOCUS       Exported                5860 bp DNA     circular SYN 02-SEP-2024
DEFINITION  High-copy episomal vector for galactose-inducible expression of 
            proteins in Saccharomyces cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    pYES2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5860)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5860)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5860)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5860
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          1..5858
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..443
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     promoter        475..493
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    501..600
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     terminator      609..856
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(1104..1692)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1866..2723)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             complement(2822..3622)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(3623..3838)
                     /label=URA3 promoter
     rep_origin      complement(4439..5316)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     rep_origin      complement(5327..5840)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 acggattaga agccgccgag cgggtgacag ccctccgaag gaagactctc ctccgtgcgt
       61 cctcgtcttc accggtcgcg ttcctgaaac gcagatgtgc ctcgcgccgc actgctccga
      121 acaataaaga ttctacaata ctagctttta tggttatgaa gaggaaaaat tggcagtaac
      181 ctggccccac aaaccttcaa atgaacgaat caaattaaca accataggat gataatgcga
      241 ttagtttttt agccttattt ctggggtaat taatcagcga agcgatgatt tttgatctat
      301 taacagatat ataaatgcaa aaactgcata accactttaa ctaatacttt caacattttc
      361 ggtttgtatt acttcttatt caaatgtaat aaaagtatca acaaaaaatt gttaatatac
      421 ctctatactt taacgtcaag gagaaaaaac cccggatcgg actactagca gctgtaatac
      481 gactcactat agggaatatt aagcttggta ccgagctcgg atccactagt aacggccgcc
      541 agtgtgctgg aattctgcag atatccatca cactggcggc cgctcgagca tgcatctaga
      601 gggccgcatc atgtaattag ttatgtcacg cttacattca cgccctcccc ccacatccgc
      661 tctaaccgaa aaggaaggag ttagacaacc tgaagtctag gtccctattt atttttttat
      721 agttatgtta gtattaagaa cgttatttat atttcaaatt tttctttttt ttctgtacag
      781 acgcgtgtac gcatgtaaca ttatactgaa aaccttgctt gagaaggttt tgggacgctc
      841 gaaggcttta atttgcaagc tgcggccctg cattaatgaa tcggccaacg cgcggggaga
      901 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
      961 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     1021 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     1081 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     1141 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     1201 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     1261 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     1321 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     1381 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     1441 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     1501 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     1561 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     1621 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     1681 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     1741 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     1801 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     1861 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     1921 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     1981 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     2041 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     2101 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     2161 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     2221 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     2281 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     2341 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     2401 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     2461 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     2521 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     2581 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     2641 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     2701 acacggaaat gttgaatact catactcttc ctttttcaat gggtaataac tgatataatt
     2761 aaattgaagc tctaatttgt gagtttagta tacatgcatt tacttataat acagtttttt
     2821 agttttgctg gccgcatctt ctcaaatatg cttcccagcc tgcttttctg taacgttcac
     2881 cctctacctt agcatccctt ccctttgcaa atagtcctct tccaacaata ataatgtcag
     2941 atcctgtaga gaccacatca tccacggttc tatactgttg acccaatgcg tctcccttgt
     3001 catctaaacc cacaccgggt gtcataatca accaatcgta accttcatct cttccaccca
     3061 tgtctctttg agcaataaag ccgataacaa aatctttgtc gctcttcgca atgtcaacag
     3121 tacccttagt atattctcca gtagataggg agcccttgca tgacaattct gctaacatca
     3181 aaaggcctct aggttccttt gttacttctt ctgccgcctg cttcaaaccg ctaacaatac
     3241 ctgggcccac cacaccgtgt gcattcgtaa tgtctgccca ttctgctatt ctgtatacac
     3301 ccgcagagta ctgcaatttg actgtattac caatgtcagc aaattttctg tcttcgaaga
     3361 gtaaaaaatt gtacttggcg gataatgcct ttagcggctt aactgtgccc tccatggaaa
     3421 aatcagtcaa gatatccaca tgtgttttta gtaaacaaat tttgggacct aatgcttcaa
     3481 ctaactccag taattccttg gtggtacgaa catccaatga agcacacaag tttgtttgct
     3541 tttcgtgcat gatattaaat agcttggcag caacaggact aggatgagta gcagcacgtt
     3601 ccttatatgt agctttcgac atgatttatc ttcgtttcct gcaggttttt gttctgtgca
     3661 gttgggttaa gaatactggg caatttcatg tttcttcaac actacatatg cgtatatata
     3721 ccaatctaag tctgtgctcc ttccttcgtt cttccttctg ttcggagatt accgaatcaa
     3781 aaaaatttca aggaaaccga aatcaaaaaa aagaataaaa aaaaaatgat gaattgaaaa
     3841 gctagcttat cgatgataag ctgtcaaaca tgagaattaa ttccacggac tatagactat
     3901 acctagtata ctccgtctac tgtacgatac acttccgctc aggtccttgt cctttaacga
     3961 ggccttacca ctcttttgtt actctattga tccagctcag caaaggcagt gtgatctaag
     4021 attctatctt cgcgatgtag taaaactagc tagaccgaga aagagactag aaatgcaaaa
     4081 ggcacttcta caatggctgc catcattatt atccgatgtg acgctgcagc ttctcaatga
     4141 tattcgaata cgctttgagg agatacagcc taatatccga caaactgttt tacagattta
     4201 cgatcgtact tgttacccat cattgaattt tgaacatccg aacctgggag ttttccctga
     4261 aacagatagt atatttgaac ctgtataata atatatagtc tagcgcttta cggaagacaa
     4321 tgtatgtatt tcggttcctg gagaaactat tgcatctatt gcataggtaa tcttgcacgt
     4381 cgcatccccg gttcattttc tgcgtttcca tcttgcactt caatagcata tctttgttaa
     4441 cgaagcatct gtgcttcatt ttgtagaaca aaaatgcaac gcgagagcgc taatttttca
     4501 aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgaaag cgctatttta
     4561 ccaacgaaga atctgtgctt catttttgta aaacaaaaat gcaacgcgag agcgctaatt
     4621 tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc gagagcgcta
     4681 ttttaccaac aaagaatcta tacttctttt ttgttctaca aaaatgcatc ccgagagcgc
     4741 tatttttcta acaaagcatc ttagattact ttttttctcc tttgtgcgct ctataatgca
     4801 gtctcttgat aactttttgc actgtaggtc cgttaaggtt agaagaaggc tactttggtg
     4861 tctattttct cttccataaa aaaagcctga ctccacttcc cgcgtttact gattactagc
     4921 gaagctgcgg gtgcattttt tcaagataaa ggcatccccg attatattct ataccgatgt
     4981 ggattgcgca tactttgtga acagaaagtg atagcgttga tgattcttca ttggtcagaa
     5041 aattatgaac ggtttcttct attttgtctc tatatactac gtataggaaa tgtttacatt
     5101 ttcgtattgt tttcgattca ctctatgaat agttcttact acaatttttt tgtctaaaga
     5161 gtaatactag agataaacat aaaaaatgta gaggtcgagt ttagatgcaa gttcaaggag
     5221 cgaaaggtgg atgggtaggt tatataggga tatagcacag agatatatag caaagagata
     5281 cttttgagca atgtttgtgg aagcggtatt cgcaatggga agctccaccc cggttgataa
     5341 tcagaaaagc cccaaaaaca ggaagattgt ataagcaaat atttaaattg taagcgttaa
     5401 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     5461 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     5521 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     5581 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     5641 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     5701 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     5761 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     5821 tgcgccgcta cagggcgcgt ggggatcgat ccactagtcc
//
