LOCUS       pYES2_NT_C.        6037 bp DNA     circular SYN 01-JAN-1980
DEFINITION  High-copy episomal vector for galactose-inducible expression of 
            N-terminally tagged proteins in S. cerevisiae. For other reading 
            frames, use pYES2/NT A or pYES2/NT B.
ACCESSION   .
VERSION     .
KEYWORDS    pYES2 NT C
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6037)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6037)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6037
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..443
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     promoter        475..493
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             510..512
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             522..539
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             543..575
                     /label=T7 tag (gene 10 leader)
                     /note="leader peptide from bacteriophage T7 gene 10"
     CDS             579..602
                     /label=Xpress(TM) tag
                     /note="Xpress(TM) epitope tag, including an enterokinase 
                     recognition and cleavage site"
     misc_feature    602..668
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             681..722
                     /label=V5 tag
                     /note="epitope tag from simian virus 5"
     CDS             732..749
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      783..1030
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(1278..1866)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2040..2897)
                     /label=AmpR
                     /note="beta-lactamase"
     CDS             complement(2996..3796)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(3797..4017)
                     /label=URA3 promoter
     rep_origin      complement(4616..5496)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     rep_origin      complement(5507..6020)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 acggattaga agccgccgag cgggtgacag ccctccgaag gaagactctc ctccgtgcgt
       61 cctcgtcttc accggtcgcg ttcctgaaac gcagatgtgc ctcgcgccgc actgctccga
      121 acaataaaga ttctacaata ctagctttta tggttatgaa gaggaaaaat tggcagtaac
      181 ctggccccac aaaccttcaa atgaacgaat caaattaaca accataggat gataatgcga
      241 ttagtttttt agccttattt ctggggtaat taatcagcga agcgatgatt tttgatctat
      301 taacagatat ataaatgcaa aaactgcata accactttaa ctaatacttt caacattttc
      361 ggtttgtatt acttcttatt caaatgtaat aaaagtatca acaaaaaatt gttaatatac
      421 ctctatactt taacgtcaag gagaaaaaac cccggatcgg actactagca gctgtaatac
      481 gactcactat agggaatatt aagcttacca tggggggttc tcatcatcat catcatcatg
      541 gtatggctag catgactggt ggacagcaaa tgggtcggga tctgtacgac gatgacgata
      601 aggtacccgg atccagtgtg gtggaattct gcagatatcc agcacagtgg cggccgctcg
      661 agtctagagg gcccttcgaa ggtaagccta tccctaaccc tctcctcggt ctcgattcta
      721 cgcgtaccgg tcatcatcac catcaccatt gagtttaaac ccgctgatcc tagagggccg
      781 catcatgtaa ttagttatgt cacgcttaca ttcacgccct ccccccacat ccgctctaac
      841 cgaaaaggaa ggagttagac aacctgaagt ctaggtccct atttattttt ttatagttat
      901 gttagtatta agaacgttat ttatatttca aatttttctt ttttttctgt acagacgcgt
      961 gtacgcatgt aacattatac tgaaaacctt gcttgagaag gttttgggac gctcgaaggc
     1021 tttaatttgc aagctgcggc cctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     1081 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     1141 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     1201 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa agcccaggaa ccgtaaaaag
     1261 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     1321 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     1381 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     1441 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     1501 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     1561 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     1621 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     1681 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     1741 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     1801 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     1861 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     1921 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     1981 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     2041 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     2101 gcctgactcc ccgtcgtgta gataactacg atacgggagc gcttaccatc tggccccagt
     2161 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     2221 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     2281 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     2341 gttggcattg ctacaggcat cgtggtgtca ctctcgtcgt ttggtatggc ttcattcagc
     2401 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     2461 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     2521 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     2581 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     2641 tgcccggcgt caatacggga taatagtgta tcacatagca gaactttaaa agtgctcatc
     2701 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     2761 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     2821 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     2881 aaatgttgaa tactcatact cttccttttt caatgggtaa taactgatat aattaaattg
     2941 aagctctaat ttgtgagttt agtatacatg catttactta taatacagtt ttttagtttt
     3001 gctggccgca tcttctcaaa tatgcttccc agcctgcttt tctgtaacgt tcaccctcta
     3061 ccttagcatc ccttcccttt gcaaatagtc ctcttccaac aataataatg tcagatcctg
     3121 tagagaccac atcatccacg gttctatact gttgacccaa tgcgtctccc ttgtcatcta
     3181 aacccacacc gggtgtcata atcaaccaat cgtaaccttc atctcttcca cccatgtctc
     3241 tttgagcaat aaagccgata acaaaatctt tgtcgctctt cgcaatgtca acagtaccct
     3301 tagtatattc tccagtagat agggagccct tgcatgacaa ttctgctaac atcaaaaggc
     3361 ctctaggttc ctttgttact tcttctgccg cctgcttcaa accgctaaca atacctgggc
     3421 ccaccacacc gtgtgcattc gtaatgtctg cccattctgc tattctgtat acacccgcag
     3481 agtactgcaa tttgactgta ttaccaatgt cagcaaattt tctgtcttcg aagagtaaaa
     3541 aattgtactt ggcggataat gcctttagcg gcttaactgt gccctccatg gaaaaatcag
     3601 tcaagatatc cacatgtgtt tttagtaaac aaattttggg acctaatgct tcaactaact
     3661 ccagtaattc cttggtggta cgaacatcca atgaagcaca caagtttgtt tgcttttcgt
     3721 gcatgatatt aaatagcttg gcagcaacag gactaggatg agtagcagca cgttccttat
     3781 atgtagcttt cgacatgatt tatcttcgtt tcctgcaggt ttttgttctg tgcagttggg
     3841 ttaagaatac tgggcaattt catgtttctt caacactaca tatgcgtata tataccaatc
     3901 taagtctgtg ctccttcctt cgttcttcct tctgttcgga gattaccgaa tcaaaaaaat
     3961 ttcaaagaaa ccgaaatcaa aaaaaagaat aaaaaaaaaa tgatgaattg aattgaaaag
     4021 ctagcttatc gatgataagc tgtcaaagat gagaattaat tccacggact atagactata
     4081 ctagatactc cgtctactgt acgatacact tccgctcagg tccttgtcct ttaacgaggc
     4141 cttaccactc ttttgttact ctattgatcc agctcagcaa aggcagtgtg atctaagatt
     4201 ctatcttcgc gatgtagtaa aactagctag accgagaaag agactagaaa tgcaaaaggc
     4261 acttctacaa tggctgccat cattattatc cgatgtgacg ctgcagcttc tcaatgatat
     4321 tcgaatacgc tttgaggaga tacagcctaa tatccgacaa actgttttac agatttacga
     4381 tcgtacttgt tacccatcat tgaattttga acatccgaac ctgggagttt tccctgaaac
     4441 agatagtata tttgaacctg tataataata tatagtctag cgctttacgg aagacaatgt
     4501 atgtatttcg gttcctggag aaactattgc atctattgca taggtaatct tgcacgtcgc
     4561 atccccggtt cattttctgc gtttccatct tgcacttcaa tagcatatct ttgttaacga
     4621 agcatctgtg cttcattttg tagaacaaaa atgcaacgcg agagcgctaa tttttcaaac
     4681 aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgaaagcgc tattttacca
     4741 acgaagaatc tgtgcttcat ttttgtaaaa caaaaatgca acgcgacgag agcgctaatt
     4801 tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc gagagcgcta
     4861 ttttaccaac aaagaatcta tacttctttt ttgttctaca aaaatgcatc ccgagagcgc
     4921 tatttttcta acaaagcatc ttagattact ttttttctcc tttgtgcgct ctataatgca
     4981 gtctcttgat aactttttgc actgtaggtc cgttaaggtt agaagaaggc tactttggtg
     5041 tctattttct cttccataaa aaaagcctga ctccacttcc cgcgtttact gattactagc
     5101 gaagctgcgg gtgcattttt tcaagataaa ggcatccccg attatattct ataccgatgt
     5161 ggattgcgca tactttgtga acagaaagtg atagcgttga tgattcttca ttggtcagaa
     5221 aattatgaac ggtttcttct attttgtctc tatatactac gtataggaaa tgtttacatt
     5281 ttcgtattgt tttcgattca ctctatgaat agttcttact acaatttttt tgtctaaaga
     5341 gtaatactag agataaacat aaaaaatgta gaggtcgagt ttagatgcaa gttcaaggag
     5401 cgaaaggtgg atgggtaggt tatataggga tatagcacag agatatatag caaagagata
     5461 cttttgagca atgtttgtgg aagcggtatt cgcaatggga agctccaccc cggttgataa
     5521 tcagaaaagc cccaaaaaca ggaagattgt ataagcaaat atttaaattg taaacgttaa
     5581 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta acgaatagcc
     5641 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     5701 tccagtttcc aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     5761 aagggtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     5821 gtcgaggtgc cgtaaagcag taaatcggaa gggtaaacgg atgcccccat ttagagcttg
     5881 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggggc
     5941 tagggcggtg ggaagtgtag gggtcacgct gggcgtaacc accacacccg ccgcgcttaa
     6001 tggggcgcta cagggcgcgt ggggatgatc cactagt
//
