LOCUS       Exported                5661 bp DNA     circular SYN 28-JUL-2025
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5661)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5661)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5661
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          4802..4808
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      18..531
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     rep_origin      542..1422
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        2021..2208
                     /label=HIS3 promoter
     CDS             2209..2868
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALLARGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHFL
                     ESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     CDS             2939..3799
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCDTLLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDESDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKRSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3970..4558
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(4806..5053)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     promoter        complement(5169..5187)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(5219..5660)
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    5543..5660
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 actagtggat catccccacg cgccctgtag cgccccatta agcgcggcgg gtgtggtggt
       61 tacgcccagc gtgaccccta cacttcccac cgccctagcc cccgctcctt tcgctttctt
      121 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatg ggggcatccg
      181 tttacccttc cgatttactg ctttacggca cctcgacccc aaaaaacttg attagggtga
      241 tggttcacgt agtgggccat cgccctgata gacccttttt cgccctttga cgttggagtc
      301 cacgttcttt aatagtggac tcttgttgga aactggaaca acactcaacc ctatctcggt
      361 ctattctttt gatttataag ggattttgcc gatttcgggc tattcgttaa aaaatgagct
      421 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa tttaaatatt
      481 tgcttataca atcttcctgt ttttggggct tttctgatta tcaaccgggg tggagcttcc
      541 cattgcgaat accgcttcca caaacattgc tcaaaagtat ctctttgcta tatatctctg
      601 tgctatatcc ctatataacc tacccatcca cctttcgctc cttgaacttg catctaaact
      661 cgacctctac attttttatg tttatctcta gtattactct ttagacaaaa aaattgtagt
      721 aagaactatt catagagtga atcgaaaaca atacgaaaat gtaaacattt cctatacgta
      781 gtatatagag acaaaataga agaaaccgtt cataattttc tgaccaatga agaatcatca
      841 acgctatcac tttctgttca caaagtatgc gcaatccaca tcggtataga atataatcgg
      901 ggatgccttt atcttgaaaa aatgcacccg cagcttcgct agtaatcagt aaacgcggga
      961 agtggagtca ggcttttttt atggaagaga aaatagacac caaagtagcc ttcttctaac
     1021 cttaacggac ctacagtgca aaaagttatc aagagactgc attatagagc gcacaaagga
     1081 gaaaaaaagt aatctaagat gctttgttag aaaaatagcg ctctcgggat gcatttttgt
     1141 agaacaaaaa agaagtatag attctttgtt ggtaaaatag cgctctcgcg ttgcatttct
     1201 gttctgtaaa aatgcagctc agattctttg tttgaaaaat tagcgctctc gtcgcgttgc
     1261 atttttgttt tacaaaaatg aagcacagat tcttcgttgg taaaatagcg ctttcgcgtt
     1321 gcatttctgt tctgtaaaaa tgcagctcag attctttgtt tgaaaaatta gcgctctcgc
     1381 gttgcatttt tgttctacaa aatgaagcac agatgcttcg ttaacaaaga tatgctattg
     1441 aagtgcaaga tggaaacgca gaaaatgaac cggggatgcg acgtgcaaga ttacctatgc
     1501 aatagatgca atagtttctc caggaaccga aatacataca ttgtcttccg taaagcgcta
     1561 gactatatat tattatacag gttcaaatat actatctgtt tcagggaaaa ctcccaggtt
     1621 cggatgttca aaattcaatg atgggtaaca agtacgatcg taaatctgta aaacagtttg
     1681 tcggatatta ggctgtatct cctcaaagcg tattcgaata tcattgagaa gctgcagcgt
     1741 cacatcggat aataatgatg gcagccattg tagaagtgcc ttttgcattt ctagtctctt
     1801 tctcggtcta gctagtttta ctacatcgcg aagatagaat cttagatcac actgcctttg
     1861 ctgagctgga tcaatagagt aacaaaagag tggtaaggcc tcgttaaagg acaaggacct
     1921 gagcggaagt gtatcgtaca gtagacggag tatactaggt atagtctata gtccgtggaa
     1981 ttaattctca tgtttgacag cttatcatcg ataagctagc ctagtacact ctatattttt
     2041 ttatgcctcg gtaatgattt tcattttttt ttttccacct agcggatgac tctttttttt
     2101 tcttagcgat tggcattatc acataatgaa ttatacatta tataaagtaa tgtgatttct
     2161 tcgaagaata tactaaaaaa tgagcaggca agataaacga aggcaaagat gacagagcag
     2221 aaagccctag taaagcgtat tacaaatgaa accaagattc agattgcgat ctctttaaag
     2281 ggtggtcccc tagcgataga gcactcgatc ttcccagaaa aagaggcaga agcagtagca
     2341 gaacaggcca cacaatcgca agtgattaac gtccacacag gtatagggtt tctggaccat
     2401 atgatacatg ctctggccaa gcattccggc tggtcgctaa tcgttgagtg cattggtgac
     2461 ttacacatag acgaccatca caccactgaa gactgcggga ttgctctcgg tcaagctttt
     2521 aaagaggccc tactggcgcg tggagtaaaa aggtttggat caggatttgc gcctttggat
     2581 gaggcacttt ccagagcggt ggtagatctt tcgaacaggc cgtacgcagt tgtcgaactt
     2641 ggtttgcaaa gggagaaagt aggagatctc tcttgcgaga tgatcccgca ttttcttgaa
     2701 agctttgcag aggctagcag aattaccctc cacgttgatt gtctgcgagg caagaatgat
     2761 catcaccgta gtgagagtgc gttcaaggct cttgcggttg ccataagaga agccacctcg
     2821 cccaatggta ccaacgatgt tccctccacc aaaggtgttc ttatgtaggt atactaaact
     2881 cacaaattag agcttcaatt taattatatc agttattacc cattgaaaaa ggaagagtat
     2941 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     3001 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     3061 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     3121 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtgatacac tattatcccg
     3181 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     3241 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     3301 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     3361 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     3421 tcgttgggaa ccggagctga atgaagccat accaaacgac gagagtgaca ccacgatgcc
     3481 tgtagcaatg ccaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     3541 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     3601 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     3661 cggtatcatt gcagcactgg ggccagatgg taagcgctcc cgtatcgtag ttatctacac
     3721 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     3781 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     3841 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     3901 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     3961 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     4021 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     4081 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     4141 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     4201 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     4261 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     4321 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     4381 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     4441 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     4501 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     4561 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     4621 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     4681 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     4741 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagggccgc
     4801 agcttgcaaa ttaaagcctt cgagcgtccc aaaaccttct caagcaaggt tttcagtata
     4861 atgttacatg cgtacacgcg tctgtacaga aaaaaaagaa aaatttgaaa tataaataac
     4921 gttcttaata ctaacataac tataaaaaaa taaataggga cctagacttc aggttgtcta
     4981 actccttcct tttcggttag agcggatgtg gggggagggc gtgaatgtaa gcgtgacata
     5041 actaattaca tgatgcggcc ctctagatgc atgctcgagc ggccgccagt gtgatggata
     5101 tctgcagaat tccagcacac tggcggccgt tactagtgga tccgagctcg gtaccaagct
     5161 taatattccc tatagtgagt cgtattacag ctgctagtag tccgatccgg ggttttttct
     5221 ccttgacgtt aaagtataga ggtatattaa caattttttg ttgatacttt tattacattt
     5281 gaataagaag taatacaaac cgaaaatgtt gaaagtatta gttaaagtgg ttatgcagtt
     5341 tttgcattta tatatctgtt aatagatcaa aaatcatcgc ttcgctgatt aattacccca
     5401 gaaataaggc taaaaaacta atcgcattat catcctatgg ttgttaattt gattcgttca
     5461 tttgaaggtt tgtggggcca ggttactgcc aatttttcct cttcataacc ataaaagcta
     5521 gtattgtaga atctttattg ttcggagcag tgcggcgcga ggcacatctg cgtttcagga
     5581 acgcgaccgg tgaagacgag gacgcacgga ggagagtctt ccttcggagg gctgtcaccc
     5641 gctcggcggc ttctaatccg t
//
