LOCUS       62056_23090        6184 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6184)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..443
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     promoter        475..493
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             513..779
                     /codon_start=1
                     /label=alpha-factor secretion signal
                     /note="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
                     /translation="MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYSDLE
                     GDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLEKREAEA"
     CDS             828..869
                     /codon_start=1
                     /label=V5 tag
                     /note="epitope tag from simian virus 5"
                     /translation="GKPIPNPLLGLDST"
     CDS             879..896
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     terminator      930..1177
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(1425..2013)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2187..3044)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCDTLLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDESDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKRSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             complement(3143..3943)
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(3944..4164)
                     /label=URA3 promoter
     rep_origin      complement(4763..5643)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     rep_origin      complement(5654..6167)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 acggattaga agccgccgag cgggtgacag ccctccgaag gaagactctc ctccgtgcgt
       61 cctcgtcttc accggtcgcg ttcctgaaac gcagatgtgc ctcgcgccgc actgctccga
      121 acaataaaga ttctacaata ctagctttta tggttatgaa gaggaaaaat tggcagtaac
      181 ctggccccac aaaccttcaa atgaacgaat caaattaaca accataggat gataatgcga
      241 ttagtttttt agccttattt ctggggtaat taatcagcga agcgatgatt tttgatctat
      301 taacagatat ataaatgcaa aaactgcata accactttaa ctaatacttt caacattttc
      361 ggtttgtatt acttcttatt caaatgtaat aaaagtatca acaaaaaatt gttaatatac
      421 ctctatactt taacgtcaag gagaaaaaac cccggatcgg actactagca gctgtaatac
      481 gactcactat agggaatatt aagcttggta ccatgagatt tccttcaatt tttactgcag
      541 ttttattcgc agcatcctcc gcattagctg ctccagtcaa cactacaaca gaagatgaaa
      601 cggcacaaat tccggctgaa gctgtcatcg gttactcaga tttagaaggg gatttcgatg
      661 ttgctgtttt gccattttcc aacagcacaa ataacgggtt attgtttata aatactacta
      721 ttgccagcat tgctgctaaa gaagaagggg tatctctcga gaaaagagag gctgaagctt
      781 acgtagaatt ccctagggcg gccgcgaatt ctagagggcc cttcgaaggt aagcctatcc
      841 ctaaccctct cctcggtctc gattctacgc gtaccggtca tcatcaccat caccattgag
      901 tttaaacccg ctgatcctag agggccgcat catgtaatta gttatgtcac gcttacattc
      961 acgccctccc cccacatccg ctctaaccga aaaggaagga gttagacaac ctgaagtcta
     1021 ggtccctatt tattttttta tagttatgtt agtattaaga acgttattta tatttcaaat
     1081 ttttcttttt tttctgtaca gacgcgtgta cgcatgtaac attatactga aaaccttgct
     1141 tgagaaggtt ttgggacgct cgaaggcttt aatttgcaag ctgcggccct gcattaatga
     1201 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     1261 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     1321 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     1381 cagcaaaagc ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     1441 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     1501 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     1561 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     1621 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     1681 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     1741 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     1801 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     1861 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     1921 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     1981 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     2041 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     2101 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     2161 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     2221 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     2281 cgggagcgct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     2341 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     2401 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     2461 tcgccagtta atagtttgcg caacgttgtt ggcattgcta caggcatcgt ggtgtcactc
     2521 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     2581 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     2641 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     2701 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     2761 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa tagtgtatca
     2821 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     2881 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     2941 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     3001 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     3061 tgggtaataa ctgatataat taaattgaag ctctaatttg tgagtttagt atacatgcat
     3121 ttacttataa tacagttttt tagttttgct ggccgcatct tctcaaatat gcttcccagc
     3181 ctgcttttct gtaacgttca ccctctacct tagcatccct tccctttgca aatagtcctc
     3241 ttccaacaat aataatgtca gatcctgtag agaccacatc atccacggtt ctatactgtt
     3301 gacccaatgc gtctcccttg tcatctaaac ccacaccggg tgtcataatc aaccaatcgt
     3361 aaccttcatc tcttccaccc atgtctcttt gagcaataaa gccgataaca aaatctttgt
     3421 cgctcttcgc aatgtcaaca gtacccttag tatattctcc agtagatagg gagcccttgc
     3481 atgacaattc tgctaacatc aaaaggcctc taggttcctt tgttacttct tctgccgcct
     3541 gcttcaaacc gctaacaata cctgggccca ccacaccgtg tgcattcgta atgtctgccc
     3601 attctgctat tctgtataca cccgcagagt actgcaattt gactgtatta ccaatgtcag
     3661 caaattttct gtcttcgaag agtaaaaaat tgtacttggc ggataatgcc tttagcggct
     3721 taactgtgcc ctccatggaa aaatcagtca agatatccac atgtgttttt agtaaacaaa
     3781 ttttgggacc taatgcttca actaactcca gtaattcctt ggtggtacga acatccaatg
     3841 aagcacacaa gtttgtttgc ttttcgtgca tgatattaaa tagcttggca gcaacaggac
     3901 taggatgagt agcagcacgt tccttatatg tagctttcga catgatttat cttcgtttcc
     3961 tgcaggtttt tgttctgtgc agttgggtta agaatactgg gcaatttcat gtttcttcaa
     4021 cactacatat gcgtatatat accaatctaa gtctgtgctc cttccttcgt tcttccttct
     4081 gttcggagat taccgaatca aaaaaatttc aaagaaaccg aaatcaaaaa aaagaataaa
     4141 aaaaaaatga tgaattgaat tgaaaagcta gcttatcgat gataagctgt caaagatgag
     4201 aattaattcc acggactata gactatacta gatactccgt ctactgtacg atacacttcc
     4261 gctcaggtcc ttgtccttta acgaggcctt accactcttt tgttactcta ttgatccagc
     4321 tcagcaaagg cagtgtgatc taagattcta tcttcgcgat gtagtaaaac tagctagacc
     4381 gagaaagaga ctagaaatgc aaaaggcact tctacaatgg ctgccatcat tattatccga
     4441 tgtgacgctg cagcttctca atgatattcg aatacgcttt gaggagatac agcctaatat
     4501 ccgacaaact gttttacaga tttacgatcg tacttgttac ccatcattga attttgaaca
     4561 tccgaacctg ggagttttcc ctgaaacaga tagtatattt gaacctgtat aataatatat
     4621 agtctagcgc tttacggaag acaatgtatg tatttcggtt cctggagaaa ctattgcatc
     4681 tattgcatag gtaatcttgc acgtcgcatc cccggttcat tttctgcgtt tccatcttgc
     4741 acttcaatag catatctttg ttaacgaagc atctgtgctt cattttgtag aacaaaaatg
     4801 caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa
     4861 atgcaacgcg aaagcgctat tttaccaacg aagaatctgt gcttcatttt tgtaaaacaa
     4921 aaatgcaacg cgacgagagc gctaattttt caaacaaaga atctgagctg catttttaca
     4981 gaacagaaat gcaacgcgag agcgctattt taccaacaaa gaatctatac ttcttttttg
     5041 ttctacaaaa atgcatcccg agagcgctat ttttctaaca aagcatctta gattactttt
     5101 tttctccttt gtgcgctcta taatgcagtc tcttgataac tttttgcact gtaggtccgt
     5161 taaggttaga agaaggctac tttggtgtct attttctctt ccataaaaaa agcctgactc
     5221 cacttcccgc gtttactgat tactagcgaa gctgcgggtg cattttttca agataaaggc
     5281 atccccgatt atattctata ccgatgtgga ttgcgcatac tttgtgaaca gaaagtgata
     5341 gcgttgatga ttcttcattg gtcagaaaat tatgaacggt ttcttctatt ttgtctctat
     5401 atactacgta taggaaatgt ttacattttc gtattgtttt cgattcactc tatgaatagt
     5461 tcttactaca atttttttgt ctaaagagta atactagaga taaacataaa aaatgtagag
     5521 gtcgagttta gatgcaagtt caaggagcga aaggtggatg ggtaggttat atagggatat
     5581 agcacagaga tatatagcaa agagatactt ttgagcaatg tttgtggaag cggtattcgc
     5641 aatgggaagc tccaccccgg ttgataatca gaaaagcccc aaaaacagga agattgtata
     5701 agcaaatatt taaattgtaa acgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     5761 aatcagctca ttttttaacg aatagcccga aatcggcaaa atcccttata aatcaaaaga
     5821 atagaccgag atagggttga gtgttgttcc agtttccaac aagagtccac tattaaagaa
     5881 cgtggactcc aacgtcaaag ggcgaaaaag ggtctatcag ggcgatggcc cactacgtga
     5941 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcagtaa atcggaaggg
     6001 taaacggatg cccccattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     6061 agggaagaaa gcgaaaggag cgggggctag ggcggtggga agtgtagggg tcacgctggg
     6121 cgtaaccacc acacccgccg cgcttaatgg ggcgctacag ggcgcgtggg gatgatccac
     6181 tagt
//
