LOCUS       Exported                5009 bp DNA     circular SYN 03-SEP-2024
DEFINITION  Exported.
ACCESSION   V012745
VERSION     .
KEYWORDS    pYD1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5009)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5009)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5009)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5009
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..443
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     promoter        475..493
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             536..796
                     /gene="AGA2"
                     /label=A-agglutinin-binding subunit
                     /note="A-agglutinin-binding subunit from Saccharomyces 
                     cerevisiae (strain ATCC 204508 / S288c). Accession#: 
                     P32781"
     CDS             860..889
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=leader peptide from bacteriophage T7 gene 10
                     /note="T7 tag (gene 10 leader)"
                     /note="promotes efficient translation in E. coli"
                     /translation="ASMTGGQQMG"
     CDS             893..916
                     /label=Xpress(TM) tag
                     /note="Xpress(TM) epitope tag, including an enterokinase 
                     recognition and cleavage site"
     CDS             995..1036
                     /label=V5 tag
                     /note="epitope tag from simian virus 5"
     CDS             1046..1063
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             complement(1437..2108)
                     /label=TRP1
                     /note="phosphoribosylanthranilate isomerase, required for 
                     tryptophan biosynthesis"
     promoter        complement(2109..2210)
                     /label=TRP1 promoter
     misc_feature    complement(2287..2790)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     promoter        2827..2931
                     /label=AmpR promoter
     CDS             2932..3789
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3963..4551
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4839..4860
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4875..4905
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4913..4929
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4937..4953
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4974..4992
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 acggattaga agccgccgag cgggtgacag ccctccgaag gaagactctc ctccgtgcgt
       61 cctcgtcttc accggtcgcg ttcctgaaac gcagatgtgc ctcgcgccgc actgctccga
      121 acaataaaga ttctacaata ctagctttta tggttatgaa gaggaaaaat tggcagtaac
      181 ctggccccac aaaccttcaa atgaacgaat caaattaaca accataggat gataatgcga
      241 ttagtttttt agccttattt ctggggtaat taatcagcga agcgatgatt tttgatctat
      301 taacagatat ataaatgcaa aaactgcata accactttaa ctaatacttt caacattttc
      361 ggtttgtatt acttcttatt caaatgtaat aaaagtatca acaaaaaatt gttaatatac
      421 ctctatactt taacgtcaag gagaaaaaac cccggatcgg actactagca gctgtaatac
      481 gactcactat agggaatatt aagctaattc cctacttcat acattttcaa ttaagatgca
      541 gttacttcgc tgtttttcaa tattttctgt tattgcttca gttttagcac aggaactgac
      601 aactatatgc gagcaaatcc cctcaccaac tttagaatcg acgccgtact ctttgtcaac
      661 gactactatt ttggccaacg ggaaggcaat gcaaggagtt tttgaatatt acaaatcagt
      721 aacgtttgtc agtaattgcg gttctcaccc ctcaacgact agcaaaggca gccccataaa
      781 cacacagtat gtttttaagc ttctgcaggc tagtggtggt ggtggttctg gtggtggtgg
      841 ttctggtggt ggtggttctg ctagcatgac tggtggacag caaatgggtc gggatctgta
      901 cgacgatgac gataaggtac caggatccag tgtggtggaa ttctgcagat atccagcaca
      961 gtggcggccg ctcgagtcta gagggccctt cgaaggtaag cctatcccta accctctcct
     1021 cggtctcgat tctacgcgta ccggtcatca tcaccatcac cattgagttt aaacccgctg
     1081 atctgataac aacagtgtag atgtaacaaa atcgactttg ttcccactgt acttttagct
     1141 cgtacaaaat acaatatact tttcatttct ccgtaaacaa catgttttcc catgtaatat
     1201 ccttttctat ttttcgttcc gttaccaact ttacacatac tttatatagc tattcacttc
     1261 tatacactaa aaaactaaga caattttaat tttgctgcct gccatatttc aatttgttat
     1321 aaattcctat aatttatcct attagtagct aaaaaaagat gaatgtgaat cgaatcctaa
     1381 gagaattggc aagtgcacaa acaatactta aataaatact actcagtaat aacctatttc
     1441 ttagcatttt tgacgaaatt tgctattttg ttagagtctt ttacaccatt tgtctccaca
     1501 cctccgctta catcaacacc aataacgcca tttaatctaa gcgcatcacc aacattttct
     1561 ggcgtcagtc caccagctaa cataaaatgt aagctctcgg ggctctcttg ccttccaacc
     1621 cagtcagaaa tcgagttcca atccaaaagt tcacctgtcc cacctgcttc tgaatcaaac
     1681 aagggaataa acgaatgagg tttctgtgaa gctgcactga gtagtatgtt gcagtctttt
     1741 ggaaatacga gtcttttaat aactggcaaa ccgaggaact cttggtattc ttgccacgac
     1801 tcatctccgt gcagttggac gatatcaatg ccgtaatcat tgaccagagc caaaacatcc
     1861 tccttaggtt gattacgaaa cacgccaacc aagtatttcg gagtgcctga actattttta
     1921 tatgctttta caagacttga aattttcctt gcaataaccg ggtcaattgt tctctttcta
     1981 ttgggcacac atataatacc cagcaagtca gcatcggaat ctagagcaca ttctgcggcc
     2041 tctgtgctct gcaagccgca aactttcacc aatggaccag aactacctgt gaaattaata
     2101 acagacatac tccaagctgc ctttgtgtgc ttaatcacgt atactcacgt gctcaatagt
     2161 caccaatgcc ctccctcttg gccctctcct tttctttttt cgaccgaatt tcttgaagac
     2221 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     2281 aggacggatc gcttgcctgt aacttacacg cgcctcgtat cttttaatga tggaataatt
     2341 tgggaattta ctctgtgttt atttattttt atgttttgta tttggatttt agaaagtaaa
     2401 taaagaaggt agaagagtta cggaatgaag aaaaaaaaat aaacaaaggt ttaaaaaatt
     2461 tcaacaaaaa gcgtacttta catatatatt tattagacaa gaaaagcaga ttaaatagat
     2521 atacattcga ttaacgataa gtaaaatgta aaatcacagg attttcgtgt gtggtcttct
     2581 acacagacaa gatgaaacaa ttcggcatta atacctgaga gcaggaagag caagataaaa
     2641 ggtagtattt gttggcgatc cccctagagt cttttacatc ttcggaaaac aaaaactatt
     2701 ttttctttaa tttctttttt tactttctat ttttaattta tatatttata ttaaaaaatt
     2761 taaattataa ttatttttat agcacgtgat gaaaaggacc caggtggcac ttttcgggga
     2821 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     2881 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     2941 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     3001 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     3061 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     3121 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
     3181 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     3241 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     3301 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     3361 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     3421 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     3481 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     3541 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     3601 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     3661 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     3721 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     3781 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     3841 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     3901 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     3961 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     4021 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     4081 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     4141 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     4201 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     4261 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     4321 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     4381 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     4441 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     4501 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     4561 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     4621 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     4681 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     4741 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     4801 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     4861 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     4921 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct cgaaattaac
     4981 cctcactaaa gggaacaaaa gctgctagt
//
