LOCUS       40924_47878        4489 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Centromeric Saccharomyces cerevisiae expression vector that serves 
            as an acceptor in the Echo(TM) Cloning System.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4489)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4489
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..443
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     promoter        475..493
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(507..540)
                     /label=loxH
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021). The loxH variant 
                     was optimized for expression constructs but remains 
                     compatible with loxP."
     terminator      571..818
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(1066..1654)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(2123..2501)
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene;
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     CDS             complement(2784..3584)
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(3585..3805)
                     /label=URA3 promoter
     misc_feature    3833..4336
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
ORIGIN
        1 acggattaga agccgccgag cgggtgacag ccctccgaag gaagactctc ctccgtgcgt
       61 cctcgtcttc accggtcgcg ttcctgaaac gcagatgtgc ctcgcgccgc actgctccga
      121 acaataaaga ttctacaata ctagctttta tggttatgaa gaggaaaaat tggcagtaac
      181 ctggccccac aaaccttcaa atgaacgaat caaattaaca accataggat gataatgcga
      241 ttagtttttt agccttattt ctggggtaat taatcagcga agcgatgatt tttgatctat
      301 taacagatat ataaatgcaa aaactgcata accactttaa ctaatacttt caacattttc
      361 ggtttgtatt acttcttatt caaatgtaat aaaagtatca acaaaaaatt gttaatatac
      421 ctctatactt taacgtcaag gagaaaaaac cccggatcgg actactagca gctgtaatac
      481 gactcactat agggaatatt aagcttatta cctcatatag catacattat acgaagttat
      541 gtttaaaccc gctgatccta gagggccgca tcatgtaatt agttatgtca cgcttacatt
      601 cacgccctcc ccccacatcc gctctaaccg aaaaggaagg agttagacaa cctgaagtct
      661 aggtccctat ttattttttt atagttatgt tagtattaag aacgttattt atatttcaaa
      721 tttttctttt ttttctgtac agacgcgtgt acgcatgtaa cattatactg aaaaccttgc
      781 ttgagaaggt tttgggacgc tcgaaggctt taatttgcaa gctgcggccc tgcattaatg
      841 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
      901 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
      961 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     1021 ccagcaaaag cccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     1081 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     1141 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     1201 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     1261 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     1321 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     1381 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     1441 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     1501 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     1561 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     1621 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     1681 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     1741 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     1801 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     1861 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     1921 acgggagcgc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     1981 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     2041 tgcaacttta tccgcctcca ttcagtctat taattgttgc cgggaagcta gagtaagtag
     2101 ttcgccagtt aatagtttgc gcaacgttgt tggcattgct acaggcatcg tggtgtcact
     2161 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     2221 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     2281 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     2341 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     2401 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata atagtgtatc
     2461 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     2521 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     2581 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     2641 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     2701 atgggtaata actgatataa ttaaattgaa gctctaattt gtgagtttag tatacatgca
     2761 tttacttata atacagtttt ttagttttgc tggccgcatc ttctcaaata tgcttcccag
     2821 cctgcttttc tgtaacgttc accctctacc ttagcatccc ttccctttgc aaatagtcct
     2881 cttccaacaa taataatgtc agatcctgta gagaccacat catccacggt tctatactgt
     2941 tgacccaatg cgtctccctt gtcatctaaa cccacaccgg gtgtcataat caaccaatcg
     3001 taaccttcat ctcttccacc catgtctctt tgagcaataa agccgataac aaaatctttg
     3061 tcgctcttcg caatgtcaac agtaccctta gtatattctc cagtagatag ggagcccttg
     3121 catgacaatt ctgctaacat caaaaggcct ctaggttcct ttgttacttc ttctgccgcc
     3181 tgcttcaaac cgctaacaat acctgggccc accacaccgt gtgcattcgt aatgtctgcc
     3241 cattctgcta ttctgtatac acccgcagag tactgcaatt tgactgtatt accaatgtca
     3301 gcaaattttc tgtcttcgaa gagtaaaaaa ttgtacttgg cggataatgc ctttagcggc
     3361 ttaactgtgc cctccatgga aaaatcagtc aagatatcca catgtgtttt tagtaaacaa
     3421 attttgggac ctaatgcttc aactaactcc agtaattcct tggtggtacg aacatccaat
     3481 gaagcacaca agtttgtttg cttttcgtgc atgatattaa atagcttggc agcaacagga
     3541 ctaggatgag tagcagcacg ttccttatat gtagctttcg acatgattta tcttcgtttc
     3601 ctgcaggttt ttgttctgtg cagttgggtt aagaatactg ggcaatttca tgtttcttca
     3661 acactacata tgcgtatata taccaatcta agtctgtgct ccttccttcg ttcttccttc
     3721 tgttcggaga ttaccgaatc aaaaaaattt caaagaaacc gaaatcaaaa aaaagaataa
     3781 aaaaaaaatg atgaattgaa ttgaaaagct agcttatcga tgggtccttt tcatcacgtg
     3841 ctataaaaat aattataatt taaatttttt aatataaata tataaattaa aaatagaaag
     3901 taaaaaaaga aattaaagaa aaaatagttt ttgttttccg aagatgtaaa agactctagg
     3961 gggatcgcca acaaatacta ccttttatct tgctcttcct gctctcaggt attaatgccg
     4021 aattgtttca tcttgtctgt gtagaagacc acacacgaaa atcctgtgat tttacatttt
     4081 acttatcgtt aatcgaatgt atatctattt aatctgcttt tcttgtctaa taaatatata
     4141 tgtaaagtac gctttttgtt gaaatttttt aaacctttgt ttattttttt ttcttcattc
     4201 cgtaactctt ctaccttctt tatttacttt ctaaaatcca aatacaaaac ataaaaataa
     4261 ataaacacag agtaaattcc caaattattc catcattaaa agatacgagg cgcgtgtaag
     4321 ttacaggcaa gcgatccgtc cgccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     4381 aggagcgggg gctagggcgg tgggaagtgt aggggtcacg ctgggcgtaa ccaccacacc
     4441 cgccgcgctt aatggggcgc tacagggcgc gtggggatga tccactagt
//
