LOCUS       pYC2_CT.        4627 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Centromeric vector with a URA3 marker for galactose-inducible 
            expression of C-terminally tagged proteins in S. cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    pYC2 CT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4627)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4627)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4627
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..443
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     promoter        475..493
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    501..594
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             607..648
                     /label=V5 tag
                     /note="epitope tag from simian virus 5"
     CDS             658..675
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      709..956
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(1204..1792)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1966..2823)
                     /label=AmpR
                     /note="beta-lactamase"
     CDS             complement(2922..3722)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(3723..3943)
                     /label=URA3 promoter
     misc_feature    3971..4474
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
ORIGIN
        1 acggattaga agccgccgag cgggtgacag ccctccgaag gaagactctc ctccgtgcgt
       61 cctcgtcttc accggtcgcg ttcctgaaac gcagatgtgc ctcgcgccgc actgctccga
      121 acaataaaga ttctacaata ctagctttta tggttatgaa gaggaaaaat tggcagtaac
      181 ctggccccac aaaccttcaa atgaacgaat caaattaaca accataggat gataatgcga
      241 ttagtttttt agccttattt ctggggtaat taatcagcga agcgatgatt tttgatctat
      301 taacagatat ataaatgcaa aaactgcata accactttaa ctaatacttt caacattttc
      361 ggtttgtatt acttcttatt caaatgtaat aaaagtatca acaaaaaatt gttaatatac
      421 ctctatactt taacgtcaag gagaaaaaac cccggatcgg actactagca gctgtaatac
      481 gactcactat agggaatatt aagcttggta ccgagctcgg atccactagt aacggccgcc
      541 agtgtgctgg aattctgcag atatccagca cagtggcggc cgctcgagtc tagagggccc
      601 ttcgaaggta agcctatccc taaccctctc ctcggtctcg attctacgcg taccggtcat
      661 catcaccatc accattgagt ttaaacccgc tgatcctaga gggccgcatc atgtaattag
      721 ttatgtcacg cttacattca cgccctcccc ccacatccgc tctaaccgaa aaggaaggag
      781 ttagacaacc tgaagtctag gtccctattt atttttttat agttatgtta gtattaagaa
      841 cgttatttat atttcaaatt tttctttttt ttctgtacag acgcgtgtac gcatgtaaca
      901 ttatactgaa aaccttgctt gagaaggttt tgggacgctc gaaggcttta atttgcaagc
      961 tgcggccctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     1021 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     1081 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     1141 gaacatgtga gcaaaaggcc agcaaaagcc caggaaccgt aaaaaggccg cgttgctggc
     1201 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     1261 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     1321 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     1381 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     1441 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     1501 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     1561 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     1621 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     1681 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     1741 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     1801 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     1861 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     1921 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     1981 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     2041 cgtgtagata actacgatac gggagcgctt accatctggc cccagtgctg caatgatacc
     2101 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     2161 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     2221 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg gcattgctac
     2281 aggcatcgtg gtgtcactct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     2341 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     2401 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     2461 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     2521 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     2581 acgggataat agtgtatcac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     2641 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     2701 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     2761 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     2821 catactcttc ctttttcaat gggtaataac tgatataatt aaattgaagc tctaatttgt
     2881 gagtttagta tacatgcatt tacttataat acagtttttt agttttgctg gccgcatctt
     2941 ctcaaatatg cttcccagcc tgcttttctg taacgttcac cctctacctt agcatccctt
     3001 ccctttgcaa atagtcctct tccaacaata ataatgtcag atcctgtaga gaccacatca
     3061 tccacggttc tatactgttg acccaatgcg tctcccttgt catctaaacc cacaccgggt
     3121 gtcataatca accaatcgta accttcatct cttccaccca tgtctctttg agcaataaag
     3181 ccgataacaa aatctttgtc gctcttcgca atgtcaacag tacccttagt atattctcca
     3241 gtagataggg agcccttgca tgacaattct gctaacatca aaaggcctct aggttccttt
     3301 gttacttctt ctgccgcctg cttcaaaccg ctaacaatac ctgggcccac cacaccgtgt
     3361 gcattcgtaa tgtctgccca ttctgctatt ctgtatacac ccgcagagta ctgcaatttg
     3421 actgtattac caatgtcagc aaattttctg tcttcgaaga gtaaaaaatt gtacttggcg
     3481 gataatgcct ttagcggctt aactgtgccc tccatggaaa aatcagtcaa gatatccaca
     3541 tgtgttttta gtaaacaaat tttgggacct aatgcttcaa ctaactccag taattccttg
     3601 gtggtacgaa catccaatga agcacacaag tttgtttgct tttcgtgcat gatattaaat
     3661 agcttggcag caacaggact aggatgagta gcagcacgtt ccttatatgt agctttcgac
     3721 atgatttatc ttcgtttcct gcaggttttt gttctgtgca gttgggttaa gaatactggg
     3781 caatttcatg tttcttcaac actacatatg cgtatatata ccaatctaag tctgtgctcc
     3841 ttccttcgtt cttccttctg ttcggagatt accgaatcaa aaaaatttca aagaaaccga
     3901 aatcaaaaaa aagaataaaa aaaaaatgat gaattgaatt gaaaagctag cttatcgatg
     3961 ggtccttttc atcacgtgct ataaaaataa ttataattta aattttttaa tataaatata
     4021 taaattaaaa atagaaagta aaaaaagaaa ttaaagaaaa aatagttttt gttttccgaa
     4081 gatgtaaaag actctagggg gatcgccaac aaatactacc ttttatcttg ctcttcctgc
     4141 tctcaggtat taatgccgaa ttgtttcatc ttgtctgtgt agaagaccac acacgaaaat
     4201 cctgtgattt tacattttac ttatcgttaa tcgaatgtat atctatttaa tctgcttttc
     4261 ttgtctaata aatatatatg taaagtacgc tttttgttga aattttttaa acctttgttt
     4321 attttttttt cttcattccg taactcttct accttcttta tttactttct aaaatccaaa
     4381 tacaaaacat aaaaataaat aaacacagag taaattccca aattattcca tcattaaaag
     4441 atacgaggcg cgtgtaagtt acaggcaagc gatccgtccg ccggcgaacg tggcgagaaa
     4501 ggaagggaag aaagcgaaag gagcgggggc tagggcggtg ggaagtgtag gggtcacgct
     4561 gggcgtaacc accacacccg ccgcgcttaa tggggcgcta cagggcgcgt ggggatgatc
     4621 cactagt
//
