LOCUS       40924_47863        6277 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pYC121, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6277)
  AUTHORS   Yanez Carrillo P, Castano Navarro I.
  TITLE     Expression vectors for C-terminal fusions with fluorescent proteins 
            and epitope tags in C. glabrata
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6277)
  AUTHORS   Yanez Carrillo P, Castano Navarro I.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-DEC-2014) Division de Biologia Molecular, Instituto 
            Potosino de Investigacion Cientifica y Tecnologica, Camino a la 
            Presa San Jose 2055 Col. Lomas 4 seccion, San Luis Potosi 78216, 
            Mexico
REFERENCE   3  (bases 1 to 6277)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6277)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-DEC-2014) Division de Biologia Molecular, Instituto Potosino de 
            Investigacion Cientifica y Tecnologica, Camino a la Presa San Jose 
            2055 Col. Lomas 4 seccion, San Luis Potosi 78216, Mexico"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6277
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        666..737
                     /label=AmpR promoter
     CDS             738..1595
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1769..2357
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2645..2666
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2681..2711
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2719..2735
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2743..2759
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2780..2798
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2815..3214
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     primer_bind     3218..3234
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3253..3282
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     primer_bind     complement(3852..3868)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      3912..4268
                     /note="region downstream of HIS3 orf in Candida glabrata
                     BG2"
                     /regulatory_class="terminator"
     promoter        complement(4283..4301)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4311..4327)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      4472..4927
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(5061..5861)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(5862..6082)
                     /label=URA3 promoter
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagcaggt tagactaaca tgcaaaaagt ttatattcat ttttgtagtt attcttgttt
      121 tcattaggat gattacacac tgtggatgtg gattaatcga tcattgttaa tttagtgtag
      181 ctactaccat ttcaaacaaa acgtaaattc ttgcagtcgt actggatctg tgaatctatt
      241 agtatatatg aattaaagta gcttgtacat attattctgt tgaatcatat cgcagagcat
      301 tggttgaaat ccaaaatata aaaatgtaat atcacaaaaa ataatactaa ttctaacatt
      361 aatgggtcag atttttagtg aatacttaaa tttataatat cgtctattta agctagcaaa
      421 tggaacaaca tttaaagtaa gaacatcata tctacatgaa aatgtatatt tcaatctgac
      481 taataacgca gagcacatct ttcagtgatg tctgtcacat gatcaaaaag aattgtattt
      541 aaataattca taataaaagc ttaaaaaatt acaataatga aaataaagta aataatgaca
      601 tgggtaagag ttccgaaata gaatcttagt gtacaaaaca aaaattgcat cattagagat
      661 ccccacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
      721 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
      781 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
      841 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
      901 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
      961 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     1021 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     1081 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     1141 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     1201 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     1261 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     1321 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     1381 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     1441 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     1501 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     1561 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     1621 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     1681 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     1741 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     1801 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     1861 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     1921 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     1981 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     2041 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     2101 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     2161 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     2221 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     2281 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     2341 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     2401 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     2461 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     2521 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     2581 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     2641 caattaatgt gagttacctc actcattagg caccccaggc tttacacttt atgcttccgg
     2701 ctcctatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     2761 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctcatag
     2821 cttcaaaatg tttctactcc ttttttactc ttccagattt tctcggactc cgcgcatcgc
     2881 cgtaccactt caaaacaccc aagcacagca tactaaattt cccctctttc ttcctctagg
     2941 gtgtcgttaa ttacccgtac taaaggtttg gaaaagaaaa aagagaccgc ctcgtttctt
     3001 tttcttcgtc gaaaaaggca ataaaaattt ttatcacgtt tctttttctt gaaaattttt
     3061 tttttgattt ttttctcttt cgatgacctc ccattgatat ttaagttaat aaacggtctt
     3121 caatttctca agtttcagtt tcatttttct tgttctatta caactttttt tacttcttgc
     3181 tcattagaaa gaaagcatag caatctaatc taagttttct agaactagtg gatcctaggg
     3241 ttaattaacg gtgaacaaaa gctaatctcc gaggaagact tgaacggtga acaaaaatta
     3301 atctcagaag aagacttgaa cggactcgac ggtgaacaaa agttgatttc tgaagaagat
     3361 ttgaacggtg aacaaaagct aatctccgag gaagacttga acggtgaaca aaaattaatc
     3421 tcagaagaag acttgaacgg actcgacggt gaacaaaagt tgatttctga agaagatttg
     3481 aacggtgaac aaaagctaat ctccgaggaa gacttgaacg gtgaacaaaa attaatctca
     3541 gaagaagact tgaacggact cgacggtgaa caaaagttga tttctgaaga agatttgaac
     3601 ggtgaacaaa agctaatctc cgaggaagac ttgaacggtg aacaaaaatt aatctcagaa
     3661 gaagacttga acggactcga cggtgaacaa aagttgattt ctgaagaaga tttgaacggt
     3721 gaacaaaagc taatctccga ggaagacttg aacggtgaac aaaaattaat caatcactag
     3781 ctagtgaatt cgcgccagat ccgaagttcc tatactttct aggggctgca ggaattcgat
     3841 atcaagctta tcgataccgt cgacctcgag aacacaaccc acagctacca ccatcaacaa
     3901 tatttatata tataacgtac acatagaaat cacacaaaca gagtatttat tcttaactac
     3961 atgaactacc atcagaccgt ctgggccact atataatgtg ccattcataa acgtgatcac
     4021 tttacgtagc aggcaacccc aggtgaaaat ttttcagcga gctgccagat tgtcaggtga
     4081 aaaactgaaa aaaacttctg ggcgatgagc ttgtggcggg aaattaagta tataaagcag
     4141 ttagtttctt ctgcttcttg tggttctggg tattcttgta atatttggaa gaatagaaaa
     4201 tcaagactac tgcctttctt ttcatattat agaggaagaa atacgcacga acacgatata
     4261 gaggtaaagg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     4321 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     4381 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     4441 cagttgcgca gcctgaatgg cgaatggcgc gacgcgccct gtagcggcgc attaagcgcg
     4501 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     4561 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4621 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4681 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     4741 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     4801 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     4861 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
     4921 acaatttcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatag
     4981 ggtaataact gatataatta aattgaagct ctaatttgtg agtttagtat acatgcattt
     5041 acttataata cagtttttta gttttgctgg ccgcatcttc tcaaatatgc ttcccagcct
     5101 gcttttctgt aacgttcacc ctctacctta gcatcccttc cctttgcaaa tagtcctctt
     5161 ccaacaataa taatgtcaga tcctgtagag accacatcat ccacggttct atactgttga
     5221 cccaatgcgt ctcccttgtc atctaaaccc acaccgggtg tcataatcaa ccaatcgtaa
     5281 ccttcatctc ttccacccat gtctctttga gcaataaagc cgataacaaa atctttgtcg
     5341 ctcttcgcaa tgtcaacagt acccttagta tattctccag tagataggga gcccttgcat
     5401 gacaattctg ctaacatcaa aaggcctcta ggttcctttg ttacttcttc tgccgcctgc
     5461 ttcaaaccgc taacaatacc tgggcccacc acaccgtgtg cattcgtaat gtctgcccat
     5521 tctgctattc tgtatacacc cgcagagtac tgcaatttga ctgtattacc aatgtcagca
     5581 aattttctgt cttcgaagag taaaaaattg tacttggcgg ataatgcctt tagcggctta
     5641 actgtgccct ccatggaaaa atcagtcaag atatccacat gtgtttttag taaacaaatt
     5701 ttgggaccta atgcttcaac taactccagt aattccttgg tggtacgaac atccaatgaa
     5761 gcacacaagt ttgtttgctt ttcgtgcatg atattaaata gcttggcagc aacaggacta
     5821 ggatgagtag cagcacgttc cttatatgta gctttcgaca tgatttatct tcgtttcctg
     5881 caggtttttg ttctgtgcag ttgggttaag aatactgggc aatttcatgt ttcttcaaca
     5941 ctacatatgc gtatatatac caatctaagt ctgtgctcct tccttcgttc ttccttctgt
     6001 tcggagatta ccgaatcaaa aaaatttcaa agaaaccgaa atcaaaaaaa agaataaaaa
     6061 aaaaatgatg aattgaattg aaaagctgtg gtatggtgca ctctcagtac aatctgctct
     6121 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     6181 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gaggtgcatg
     6241 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcga
//
