LOCUS       40924_47838        7305 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pYBA-1359, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7305)
  AUTHORS   Yao L, Yan X, Wang H, Cao M, Robaglia C.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-NOV-2014) Beijing Agro-Biotechnology Research Center, 
            Beijing Academy of Agriculture and Forestry Sciences, 9 Shuguang 
            Huayuan Zhonglu, Beijing 100097, P.R. China
REFERENCE   2  (bases 1 to 7305)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7305)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (16-NOV-2014) Beijing Agro-Biotechnology Research Center, Beijing 
            Academy of Agriculture and Forestry Sciences, 9 Shuguang Huayuan 
            Zhonglu, Beijing 100097, P.R. China"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7305
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      741..1510
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             1511..2170
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     misc_feature    2368..2391
                     /label=RB 'overdrive' sequence
                     /note="RB 'overdrive' sequence"
     misc_feature    complement(2392..2416)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    2428..2628
                     /note="CaMV poly-A signal; derived from pRT103"
     primer_bind     2662..2678
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2712..2728)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(2729..4378)
                     /label=luciferase
                     /note="firefly luciferase"
     misc_feature    complement(4390..4445)
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     promoter        complement(4456..4801)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      complement(4903..5155)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(5159..5707)
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
     promoter        complement(5716..5899)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(6034..6058)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(6248..7039)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag ctagcttgag
      601 tatactcact agactttgct tcgcaaagtc gtgaccgcct acggcggctg cggcgcccta
      661 cgggcttgct ctccgggctt cgccctgcgc ggtcgctgcg ctcccttgcc agcccgtgga
      721 tatgtggacg atggccgcga gcggccaccg gctggctcgc ttcgctcggc ccgtggacaa
      781 ccctgctgga caagctgatg gacaggctgc gcctgcccac gagcttgacc acagggattg
      841 cccaccggct acccagcctt cgaccacata cccaccggct ccaactgcgc ggcctgcggc
      901 cttgccccat caattttttt aattttctct ggggaaaagc ctccggcctg cggcctgcgc
      961 gcttcgcttg ccggttggac accaagtgga aggcgggtca aggctcgcgc agcgaccgcg
     1021 cagcggcttg gccttgacgc gcctggaacg acccaagcct atgcgagtgg gggcagtcga
     1081 aggcgaagcc cgcccgcctg ccccccgagc ctcacggcgg cgagtgcggg ggttccaagg
     1141 gggcagcgcc accttgggca aggccgaagg ccgcgcagtc gatcaacaag ccccggaggg
     1201 gccacttttt gccggagggg gagccgcgcc gaaggcgtgg gggaaccccg caggggtgcc
     1261 cttctttggg caccaaagaa ctagatatag ggcgaaatgc gaaagactta aaaatcaaca
     1321 acttaaaaaa ggggggtacg caacagctca ttgcggcacc ccccgcaata gctcattgcg
     1381 taggttaaag aaaatctgta attgactgcc acttttacgc aacgcataat tgttgtcgcg
     1441 ctgccgaaaa gttgcagctg attgcgcatg gtgccgcaac cgtgcggcac cctaccgcat
     1501 ggagataagc atggccacgc agtccagaga aatcggcatt caagccaaga acaagcccgg
     1561 tcactgggtg caaacggaac gcaaagcgca tgaggcgtgg gccgggctta ttgcgaggaa
     1621 acccacggcg gcaatgctgc tgcatcacct cgtggcgcag atgggccacc agaacgccgt
     1681 ggtggtcagc cagaagacac tttccaagct catcggacgt tctttgcgga cggtccaata
     1741 cgcagtcaag gacttggtgg ccgagcgctg gatctccgtc gtgaagctca acggccccgg
     1801 caccgtgtcg gcctacgtgg tcaatgaccg cgtggcgtgg ggccagcccc gcgaccagtt
     1861 gcgcctgtcg gtgttcagtg ccgccgtggt ggttgatcac gacgaccagg acgaatcgct
     1921 gttggggcat ggcgacctgc gccgcatccc gaccctgtat ccgggcgagc agcaactacc
     1981 gaccggcccc ggcgaggagc cgcccagcca gcccggcatt ccgggcatgg aaccagacct
     2041 gccagccttg accgaaacgg aggaatggga acggcgcggg cagcagcgcc tgccgatgcc
     2101 cgatgagccg tgttttctgg acgatggcga gccgttggag ccgccgacac gggtcacgct
     2161 gccgcgccgg tagcacttgg gttgcgcagc aacccgtaag tgcgctgttc cagactatcg
     2221 gctgtagccg cctcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat
     2281 ggagccgggc cacctcgacc tgaatggaag ccggcggcac ctcgctaacg gattcaccgt
     2341 ttttatcagg ctctgggagg cagaatacaa acaaacacat acagcgactt agtttacccg
     2401 ccaatatatc ctgtcaacgc gtttaaactg gattttggtt ttaggaatta gaaattttat
     2461 tgatagaagt attttacaaa tacaaataca tactaagggt ttcttatatg ctcaacacat
     2521 gagcgaaacc ctataagaac cctaattccc ttatctggga actactcaca cattattctg
     2581 gagaaaaata gagagagata gatttgtaga gagagactgg tgatttgcta gttagtcatc
     2641 gcgaggtacc gggccccccc tcgaggtcga cggtatcgat aagcttgata tcgaattcct
     2701 gcagcccggg ggatccacta gttctagaca cggcgatctt tccgcccttc ttggccttta
     2761 tgaggatctc tctgattttt cttgcgtcga gttttccggt aagacctttc ggtacttcgt
     2821 ccacaaacac aactcctccg cgcaactttt tcgcggttgt tacttgactg gcgacgtaat
     2881 ccacgatctc tttttccgtc atcgtctttc cgtgctccaa aacaacaacg gcggcgggaa
     2941 gttcaccggc gtcatcgtcg ggaagacctg cgacacctgc gtcgaagatg ttggggtgtt
     3001 ggagcaagat ggattccaat tcagcgggag ccacctgata gcctttgtac ttaatcagag
     3061 acttcaggcg gtcaacgatg aagaagtgtt cgtcttcgtc ccagtaagct atgtctccag
     3121 aatgtagcca tccatccttg tcaatcaagg cgttggtcgc ttccggattg tttacataac
     3181 cggacataat cataggacct ctcacacaca gttcgcctct ttgattaacg cccagcgttt
     3241 tcccggtatc cagatccaca accttcgctt caaaaaatgg aacaacttta ccgaccgcgc
     3301 ccggtttatc atccccctcg ggtgtaatca gaatagctga tgtagtctca gtgagcccat
     3361 atccttgcct gatacctggc agatggaacc tcttggcaac cgcttccccg acttccttag
     3421 agaggggagc gccaccagaa gcaatttcgt gtaaattaga taaatcgtat ttgtcaatca
     3481 gagtgctttt ggcgaagaag gagaataggg ttggcaccag cagcgcactt tgaatcttgt
     3541 aatcctgaag gctcctcaga aacagctctt cttcaaatct atacattaag acgactcgaa
     3601 atccacatat caaatatccg agtgtagtaa acattccaaa accgtgatgg aatggaacaa
     3661 cacttaaaat cgcagtatcc ggaatgattt gattgccaaa aataggatct ctggcatgcg
     3721 agaatctcac gcaggcagtt ctatgaggca gagcgacacc tttaggcaga ccagtagatc
     3781 cagaggagtt catgatcagt gcaattgtct tgtccctatc gaaggactct ggcacaaaat
     3841 cgtattcatt aaaaccggga ggtagatgag atgtgacgaa cgtgtacatc gactgaaatc
     3901 cctggtaatc cgttttagaa tccatgataa taattttttg gatgattggg agcttttttt
     3961 gcacgttcaa aattttttgc aacccctttt tggaaacgaa caccacggta ggctgcgaaa
     4021 tgcccatact gttgagcaat tcacgttcat tataaatgtc gttcgcgggc gcaactgcaa
     4081 ctccgataaa taacgcgccc aacaccggca taaagaattg aagagagttt tcactgcata
     4141 cgacgattct gtgatttgta ttcagcccat atcgtttcat agcttctgcc aaccgaacgg
     4201 acatttcgaa gtactcagcg taagtgatgt ccacctcgat atgtgcatct gtaaaagcaa
     4261 ttgttccagg aaccagggcg tatctcttca tagccttatg cagttgctct ccagcggttc
     4321 catcttccag cggatagaat ggcgccgggc ctttctttat gtttttggcg tcttccatga
     4381 gctccaattg taattgtaaa tagtaattgt aatgttgttt gttgtttgtt gttgttggta
     4441 attgttgtaa aaatatgtcc tctccaaatg aaatgaactt ccttatatag aggaagggtc
     4501 ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatgt cacatcaatc
     4561 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg
     4621 gggtccatct ttgggaccac tgtcggcaga gagatcttga atgatagcct ttcctttatc
     4681 gcaatgatgg catttgtagg agccaccttc cttttctact gtcctttcga tgaagtgaca
     4741 gatagctggg caatggaatc cgaggaggtt tcccgaaatt atcctttgtt gaaaagtctc
     4801 aatagccctt tggtcttctg agactgtatc tttgacattt ttggagtaga ccagagtgtc
     4861 gtgctccacc atgttaccgg tcgtacgagg cctattaatc ccgatctagt aacatagatg
     4921 acaccgcgcg cgataattta tcctagtttg cgcgctatat tttgttttct atcgcgtatt
     4981 aaatgtataa ttgcgggact ctaatcataa aaacccatct cataaataac gtcatgcatt
     5041 acatgttaat tattacatgc ttaacgtaat tcaacagaaa ttatatgata atcatcgcaa
     5101 gaccggcaac aggattcaat cttaagaaac tttattgcca aatgtttgaa cgatctcaga
     5161 tctcggtgac gggcaggacc ggacggggcg gaaccggcag gctgaagtcc agctgccaga
     5221 aacccacgtc atgccagttc ccgtgcttga agccggccgc ccgcagcatg ccgcgggggg
     5281 catatccgag cgcctcgtgc atgcgcacgc tcgggtcgtt gggcagcccg atgacagcga
     5341 ccacgctctt gaagccctgt gcctccaggg acttcagcag gtgggtgtag agcgtggagc
     5401 ccagtcccgt ccgctggtgg cggggggaga cgtacacggt agactcggcc gtccagtcgt
     5461 aggcgttgcg tgccttccag gggccagcgt aggcgatgcc ggcgacctcg ccgtccacct
     5521 cggcgacgag ccagggatag cgctcccgca gacggacgag gtcgtccgtc cactcctgcg
     5581 gttcctgcgg ctcggtacgg aagttgaccg tgcttgtctc gatgtagtgg ttgacgatgg
     5641 tgcagaccgc cggcatgtcc gcctcggtgg cacggcggat gtcggccggg cgtcgttctg
     5701 ggctcataga tccggtgcag attatttgga ttgagagtga atatgagact ctaattggat
     5761 accgagggga atttatggaa cgtcagtgga gcatttttga caagaaatat ttgctagctg
     5821 atagtgacct taggcgactt ttgaacgcgc aataatggtt tctgacgtat gtgcttagct
     5881 cattaaactc cagaaacccg cggctgagtg gctccttcaa cgttgcggtt ctgtcagttc
     5941 caaacgtaaa acggcttgtc ccgcgtcatc ggcgggggtc ataacgtgac tcccttaatt
     6001 ctccgctcat gatcggcgcg ccacgtgacg cgtgtttaca ccacaatata tcctgccacg
     6061 atttgcccat gggggtgggc gaagaactcc agcatgagat ccccgcgctg gaggatcatc
     6121 cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc ggcggtggaa
     6181 tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa ccccagagtc
     6241 ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg
     6301 cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct tcagcaatat
     6361 cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg ccacagtcga
     6421 tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca tcgccatggg
     6481 tcacgacgag atcctcgccg tcgggcatgc gcgccttgag cctggcgaac agttcggctg
     6541 gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg gcttccatcc
     6601 gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag gtagccggat
     6661 caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg gcaggagcaa
     6721 ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag tcccttcccg
     6781 cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc agccacgata
     6841 gccgcgctgc ctcgtcctga agttcattca gggcaccgga caggtcggtc ttgacaaaaa
     6901 gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag ccgattgtct
     6961 gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa cctgcgtgca
     7021 atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca gatcttgatc
     7081 ccctgcgcca tcagatcctt ggcggcaaga aagccatcca gtttactttg cagggcttcc
     7141 caaccttacc agagggcgcc ccagctggca attccggttc gcttgctgtc cataaaaccg
     7201 cccagtctag ctatcgccat gtaagcccac tgcaagctac ctgctttctc tttgcgcttg
     7261 cgttttccct tgtccagata gcccagtagc tgacattcat cccag
//
