LOCUS       40924_47823        6357 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pYBA-1356, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6357)
  AUTHORS   Yao L, Yan X, Wang H, Cao M, Robaglia C.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-NOV-2014) Beijing Agro-Biotechnology Research Center, 
            Beijing Academy of Agriculture and Forestry Sciences, 9 Shuguang 
            Huayuan Zhonglu, Beijing 100097, P.R. China
REFERENCE   2  (bases 1 to 6357)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6357)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (16-NOV-2014) Beijing Agro-Biotechnology Research Center, Beijing 
            Academy of Agriculture and Forestry Sciences, 9 Shuguang Huayuan 
            Zhonglu, Beijing 100097, P.R. China"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6357
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      741..1510
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             1511..2170
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     misc_feature    2368..2391
                     /label=RB 'overdrive' sequence
                     /note="RB 'overdrive' sequence"
     misc_feature    complement(2392..2416)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    2428..2628
                     /note="CaMV poly-A signal; derived from pRT103"
     primer_bind     2662..2678
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2712..2728)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(2729..3436)
                     /label=mTFP1
                     /note="monomeric teal (cyan) fluorescent protein (Ai et
                     al., 2006)"
     misc_feature    complement(3442..3497)
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     promoter        complement(3508..3853)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      complement(3955..4207)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4211..4759)
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
     promoter        complement(4768..4951)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(5086..5110)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(5300..6091)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag ctagcttgag
      601 tatactcact agactttgct tcgcaaagtc gtgaccgcct acggcggctg cggcgcccta
      661 cgggcttgct ctccgggctt cgccctgcgc ggtcgctgcg ctcccttgcc agcccgtgga
      721 tatgtggacg atggccgcga gcggccaccg gctggctcgc ttcgctcggc ccgtggacaa
      781 ccctgctgga caagctgatg gacaggctgc gcctgcccac gagcttgacc acagggattg
      841 cccaccggct acccagcctt cgaccacata cccaccggct ccaactgcgc ggcctgcggc
      901 cttgccccat caattttttt aattttctct ggggaaaagc ctccggcctg cggcctgcgc
      961 gcttcgcttg ccggttggac accaagtgga aggcgggtca aggctcgcgc agcgaccgcg
     1021 cagcggcttg gccttgacgc gcctggaacg acccaagcct atgcgagtgg gggcagtcga
     1081 aggcgaagcc cgcccgcctg ccccccgagc ctcacggcgg cgagtgcggg ggttccaagg
     1141 gggcagcgcc accttgggca aggccgaagg ccgcgcagtc gatcaacaag ccccggaggg
     1201 gccacttttt gccggagggg gagccgcgcc gaaggcgtgg gggaaccccg caggggtgcc
     1261 cttctttggg caccaaagaa ctagatatag ggcgaaatgc gaaagactta aaaatcaaca
     1321 acttaaaaaa ggggggtacg caacagctca ttgcggcacc ccccgcaata gctcattgcg
     1381 taggttaaag aaaatctgta attgactgcc acttttacgc aacgcataat tgttgtcgcg
     1441 ctgccgaaaa gttgcagctg attgcgcatg gtgccgcaac cgtgcggcac cctaccgcat
     1501 ggagataagc atggccacgc agtccagaga aatcggcatt caagccaaga acaagcccgg
     1561 tcactgggtg caaacggaac gcaaagcgca tgaggcgtgg gccgggctta ttgcgaggaa
     1621 acccacggcg gcaatgctgc tgcatcacct cgtggcgcag atgggccacc agaacgccgt
     1681 ggtggtcagc cagaagacac tttccaagct catcggacgt tctttgcgga cggtccaata
     1741 cgcagtcaag gacttggtgg ccgagcgctg gatctccgtc gtgaagctca acggccccgg
     1801 caccgtgtcg gcctacgtgg tcaatgaccg cgtggcgtgg ggccagcccc gcgaccagtt
     1861 gcgcctgtcg gtgttcagtg ccgccgtggt ggttgatcac gacgaccagg acgaatcgct
     1921 gttggggcat ggcgacctgc gccgcatccc gaccctgtat ccgggcgagc agcaactacc
     1981 gaccggcccc ggcgaggagc cgcccagcca gcccggcatt ccgggcatgg aaccagacct
     2041 gccagccttg accgaaacgg aggaatggga acggcgcggg cagcagcgcc tgccgatgcc
     2101 cgatgagccg tgttttctgg acgatggcga gccgttggag ccgccgacac gggtcacgct
     2161 gccgcgccgg tagcacttgg gttgcgcagc aacccgtaag tgcgctgttc cagactatcg
     2221 gctgtagccg cctcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat
     2281 ggagccgggc cacctcgacc tgaatggaag ccggcggcac ctcgctaacg gattcaccgt
     2341 ttttatcagg ctctgggagg cagaatacaa acaaacacat acagcgactt agtttacccg
     2401 ccaatatatc ctgtcaacgc gtttaaactg gattttggtt ttaggaatta gaaattttat
     2461 tgatagaagt attttacaaa tacaaataca tactaagggt ttcttatatg ctcaacacat
     2521 gagcgaaacc ctataagaac cctaattccc ttatctggga actactcaca cattattctg
     2581 gagaaaaata gagagagata gatttgtaga gagagactgg tgatttgcta gttagtcatc
     2641 gcgaggtacc gggccccccc tcgaggtcga cggtatcgat aagcttgata tcgaattcct
     2701 gcagcccggg ggatccacta gttctagact tataaagttc atccatacca tcagtagaat
     2761 ttctagcaac agcagattcg taaacagtaa ccttattgta atccttatca tggttaagaa
     2821 tttcgattct atgatcaacg aaatggtaat caggcaactt aacagccttc ttagctctgt
     2881 agatagtctt aaaatcaact ctatgatgtc ctcctccttc aagaagcaac ttatgcttaa
     2941 catctccctt aagaactcca tctctaacat acattctttc agtagaagca tcccatccag
     3001 tagtcttctt ttgcataaca ggtccgttag gaggaaagtt ttctccctta agatggattt
     3061 cgtagatgaa agaatcttct tccatagaga tatcagactt aaccttaacg attcccttat
     3121 cttcgaaagt catagttctt tcccaagagt atccttcagg aaaagattgc ttgaagtagt
     3181 tagggatatc atcagggtac ttagtaaaag ctctgtttcc ataagcgaaa gcagtagtaa
     3241 ggatatcata agaaaaagga agaggagctc cttccttaac ttcaagatta atagtattag
     3301 ttccatcata aggctttcct tctccttctc cttcaataac aaaagcatgt ccgttaacat
     3361 ttccttccat cttaagtttg atcttcatat caggcttaat aactcccata gtagtttctt
     3421 ctcccttaga aaccatcaat tgtaattgta aatagtaatt gtaatgttgt ttgttgtttg
     3481 ttgttgttgg taattgttgt aaaaatatgt cctctccaaa tgaaatgaac ttccttatat
     3541 agaggaaggg tcttgcgaag gatagtggga ttgtgcgtca tcccttacgt cagtggagat
     3601 gtcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt ccacgatgct
     3661 cctcgtgggt gggggtccat ctttgggacc actgtcggca gagagatctt gaatgatagc
     3721 ctttccttta tcgcaatgat ggcatttgta ggagccacct tccttttcta ctgtcctttc
     3781 gatgaagtga cagatagctg ggcaatggaa tccgaggagg tttcccgaaa ttatcctttg
     3841 ttgaaaagtc tcaatagccc tttggtcttc tgagactgta tctttgacat ttttggagta
     3901 gaccagagtg tcgtgctcca ccatgttacc ggtcgtacga ggcctattaa tcccgatcta
     3961 gtaacataga tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat attttgtttt
     4021 ctatcgcgta ttaaatgtat aattgcggga ctctaatcat aaaaacccat ctcataaata
     4081 acgtcatgca ttacatgtta attattacat gcttaacgta attcaacaga aattatatga
     4141 taatcatcgc aagaccggca acaggattca atcttaagaa actttattgc caaatgtttg
     4201 aacgatctca gatctcggtg acgggcagga ccggacgggg cggaaccggc aggctgaagt
     4261 ccagctgcca gaaacccacg tcatgccagt tcccgtgctt gaagccggcc gcccgcagca
     4321 tgccgcgggg ggcatatccg agcgcctcgt gcatgcgcac gctcgggtcg ttgggcagcc
     4381 cgatgacagc gaccacgctc ttgaagccct gtgcctccag ggacttcagc aggtgggtgt
     4441 agagcgtgga gcccagtccc gtccgctggt ggcgggggga gacgtacacg gtagactcgg
     4501 ccgtccagtc gtaggcgttg cgtgccttcc aggggccagc gtaggcgatg ccggcgacct
     4561 cgccgtccac ctcggcgacg agccagggat agcgctcccg cagacggacg aggtcgtccg
     4621 tccactcctg cggttcctgc ggctcggtac ggaagttgac cgtgcttgtc tcgatgtagt
     4681 ggttgacgat ggtgcagacc gccggcatgt ccgcctcggt ggcacggcgg atgtcggccg
     4741 ggcgtcgttc tgggctcata gatccggtgc agattatttg gattgagagt gaatatgaga
     4801 ctctaattgg ataccgaggg gaatttatgg aacgtcagtg gagcattttt gacaagaaat
     4861 atttgctagc tgatagtgac cttaggcgac ttttgaacgc gcaataatgg tttctgacgt
     4921 atgtgcttag ctcattaaac tccagaaacc cgcggctgag tggctccttc aacgttgcgg
     4981 ttctgtcagt tccaaacgta aaacggcttg tcccgcgtca tcggcggggg tcataacgtg
     5041 actcccttaa ttctccgctc atgatcggcg cgccacgtga cgcgtgttta caccacaata
     5101 tatcctgcca cgatttgccc atgggggtgg gcgaagaact ccagcatgag atccccgcgc
     5161 tggaggatca tccagccggc gtcccggaaa acgattccga agcccaacct ttcatagaag
     5221 gcggcggtgg aatcgaaatc tcgtgatggc aggttgggcg tcgcttggtc ggtcatttcg
     5281 aaccccagag tcccgctcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg
     5341 aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct
     5401 cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc
     5461 ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg
     5521 catcgccatg ggtcacgacg agatcctcgc cgtcgggcat gcgcgccttg agcctggcga
     5581 acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac
     5641 cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc
     5701 aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct
     5761 cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc aatagcagcc
     5821 agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg
     5881 ccagccacga tagccgcgct gcctcgtcct gaagttcatt cagggcaccg gacaggtcgg
     5941 tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc
     6001 agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag
     6061 aacctgcgtg caatccatct tgttcaatca tgcgaaacga tcctcatcct gtctcttgat
     6121 cagatcttga tcccctgcgc catcagatcc ttggcggcaa gaaagccatc cagtttactt
     6181 tgcagggctt cccaacctta ccagagggcg ccccagctgg caattccggt tcgcttgctg
     6241 tccataaaac cgcccagtct agctatcgcc atgtaagccc actgcaagct acctgctttc
     6301 tctttgcgct tgcgttttcc cttgtccaga tagcccagta gctgacattc atcccag
//
