LOCUS       40924_47798        7458 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pYBA-1351, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7458)
  AUTHORS   Yao L, Yan X, Wang H, Cao M, Robaglia C.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-NOV-2014) Beijing Agro-Biotechnology Research Center, 
            Beijing Academy of Agriculture and Forestry Sciences, 9 Shuguang 
            Huayuan Zhonglu, Beijing 100097, P.R. China
REFERENCE   2  (bases 1 to 7458)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7458)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (16-NOV-2014) Beijing Agro-Biotechnology Research Center, Beijing 
            Academy of Agriculture and Forestry Sciences, 9 Shuguang Huayuan 
            Zhonglu, Beijing 100097, P.R. China"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7458
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      741..1510
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             1511..2170
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     misc_feature    2368..2391
                     /label=RB 'overdrive' sequence
                     /note="RB 'overdrive' sequence"
     misc_feature    complement(2392..2416)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    2428..2628
                     /note="CaMV poly-A signal; derived from pRT103"
     primer_bind     2662..2678
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2712..2728)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(2729..4537)
                     /label=GUS
                     /note="beta-glucuronidase"
     misc_feature    complement(4543..4598)
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     promoter        complement(4609..4954)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      complement(5056..5308)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(5312..5860)
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
     promoter        complement(5869..6052)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(6187..6211)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(6401..7192)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag ctagcttgag
      601 tatactcact agactttgct tcgcaaagtc gtgaccgcct acggcggctg cggcgcccta
      661 cgggcttgct ctccgggctt cgccctgcgc ggtcgctgcg ctcccttgcc agcccgtgga
      721 tatgtggacg atggccgcga gcggccaccg gctggctcgc ttcgctcggc ccgtggacaa
      781 ccctgctgga caagctgatg gacaggctgc gcctgcccac gagcttgacc acagggattg
      841 cccaccggct acccagcctt cgaccacata cccaccggct ccaactgcgc ggcctgcggc
      901 cttgccccat caattttttt aattttctct ggggaaaagc ctccggcctg cggcctgcgc
      961 gcttcgcttg ccggttggac accaagtgga aggcgggtca aggctcgcgc agcgaccgcg
     1021 cagcggcttg gccttgacgc gcctggaacg acccaagcct atgcgagtgg gggcagtcga
     1081 aggcgaagcc cgcccgcctg ccccccgagc ctcacggcgg cgagtgcggg ggttccaagg
     1141 gggcagcgcc accttgggca aggccgaagg ccgcgcagtc gatcaacaag ccccggaggg
     1201 gccacttttt gccggagggg gagccgcgcc gaaggcgtgg gggaaccccg caggggtgcc
     1261 cttctttggg caccaaagaa ctagatatag ggcgaaatgc gaaagactta aaaatcaaca
     1321 acttaaaaaa ggggggtacg caacagctca ttgcggcacc ccccgcaata gctcattgcg
     1381 taggttaaag aaaatctgta attgactgcc acttttacgc aacgcataat tgttgtcgcg
     1441 ctgccgaaaa gttgcagctg attgcgcatg gtgccgcaac cgtgcggcac cctaccgcat
     1501 ggagataagc atggccacgc agtccagaga aatcggcatt caagccaaga acaagcccgg
     1561 tcactgggtg caaacggaac gcaaagcgca tgaggcgtgg gccgggctta ttgcgaggaa
     1621 acccacggcg gcaatgctgc tgcatcacct cgtggcgcag atgggccacc agaacgccgt
     1681 ggtggtcagc cagaagacac tttccaagct catcggacgt tctttgcgga cggtccaata
     1741 cgcagtcaag gacttggtgg ccgagcgctg gatctccgtc gtgaagctca acggccccgg
     1801 caccgtgtcg gcctacgtgg tcaatgaccg cgtggcgtgg ggccagcccc gcgaccagtt
     1861 gcgcctgtcg gtgttcagtg ccgccgtggt ggttgatcac gacgaccagg acgaatcgct
     1921 gttggggcat ggcgacctgc gccgcatccc gaccctgtat ccgggcgagc agcaactacc
     1981 gaccggcccc ggcgaggagc cgcccagcca gcccggcatt ccgggcatgg aaccagacct
     2041 gccagccttg accgaaacgg aggaatggga acggcgcggg cagcagcgcc tgccgatgcc
     2101 cgatgagccg tgttttctgg acgatggcga gccgttggag ccgccgacac gggtcacgct
     2161 gccgcgccgg tagcacttgg gttgcgcagc aacccgtaag tgcgctgttc cagactatcg
     2221 gctgtagccg cctcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat
     2281 ggagccgggc cacctcgacc tgaatggaag ccggcggcac ctcgctaacg gattcaccgt
     2341 ttttatcagg ctctgggagg cagaatacaa acaaacacat acagcgactt agtttacccg
     2401 ccaatatatc ctgtcaacgc gtttaaactg gattttggtt ttaggaatta gaaattttat
     2461 tgatagaagt attttacaaa tacaaataca tactaagggt ttcttatatg ctcaacacat
     2521 gagcgaaacc ctataagaac cctaattccc ttatctggga actactcaca cattattctg
     2581 gagaaaaata gagagagata gatttgtaga gagagactgg tgatttgcta gttagtcatc
     2641 gcgaggtacc gggccccccc tcgaggtcga cggtatcgat aagcttgata tcgaattcct
     2701 gcagcccggg ggatccacta gttctagatt gtttgcctcc ctgctgcggt ttttcaccga
     2761 agttcatgcc agtccagcgt ttttgcagca gaaaagccgc cgacttcggt ttgcggtcgc
     2821 gagtgaagat ccctttcttg ttaccgccaa cgcgcaatat gccttgcgag gtcgcaaaat
     2881 cggcgaaatt ccatacctgt tcaccgacga cggcgctgac gcgatcaaag acgcggtgat
     2941 acatatccag ccatgcacac tgatactctt cactccacat gtcggtgtac attgagtgca
     3001 gcccggctaa cgtatccacg ccgtattcgg tgatgataat cggctgatgc agtttctcct
     3061 gccaggccag aagttctttt tccagtacct tctctgccgt ttccaaatcg ccgctttgga
     3121 cataccatcc gtaataacgg ttcaggcaca gcacatcaaa gagatcgctg atggtatcgg
     3181 tgtgagcgtc gcagaacatt acattgacgc aggtgatcgg acgcgtcggg tcgagtttac
     3241 gcgttgcttc cgccagtggc gcgaaatatt cccgtgcacc ttgcggacgg gtatccggtt
     3301 cgttggcaat actccacatc accacgcttg ggtggttttt gtcacgcgct atcagctctt
     3361 taatcgcctg taagtgcgct tgctgagttt ccccgttgac tgcctcttcg ctgtacagtt
     3421 ctttcggctt gttgcccgct tcgaaaccaa tgcctaaaga gaggttaaag ccgacagcag
     3481 cagtttcatc aatcaccacg atgccatgtt catctgccca gtcgagcatc tcttcagcgt
     3541 aagggtaatg cgaggtacgg taggagttgg ccccaatcca gtccattaat gcgtggtcgt
     3601 gcaccatcag cacgttatcg aatcctttgc cacgcaagtc cgcatcttca tgacgaccaa
     3661 agccagtaaa gtagaacggt ttgtggttaa tcaggaactg ttcgcccttc actgccactg
     3721 accggatgcc gacgcgaagc gggtagatat cacactctgt ctggcttttg gctgtgacgc
     3781 acagttcata gagataacct tcacccggtt gccagaggtg cggattcacc acttgcaaag
     3841 tcccgctagt gccttgtcca gttgcaacca cctgttgatc cgcatcacgc agttcaacgc
     3901 tgacatcacc attggccacc acctgccagt caacagacgc gtggttacag tcttgcgcga
     3961 catgcgtcac cacggtgata tcgtccaccc aggtgttcgg cgtggtgtag agcattacgc
     4021 tgcgatggat tccggcatag ttaaagaaat catggaagta agactgcttt ttcttgccgt
     4081 tttcgtcggt aatcaccatt cccggcggga tagtctgcca gttcagttcg ttgttcacac
     4141 aaacggtgat acgtacactt ttcccggcaa taacatacgg cgtgacatcg gcttcaaatg
     4201 gcgtatagcc gccctgatgc tccatcactt cctgattatt gacccacact ttgccgtaat
     4261 gagtgaccgc atcgaaacgc agcacgatac gctggcctgc ccaacctttc ggtataaaga
     4321 cttcgcgctg ataccagacg ttgcccgcat aattacgaat atctgcatcg gcgaactgat
     4381 cgttaaaact gcctggcaca gcaattgccc ggctttcttg taacgcgctt tcccaccaac
     4441 gctgatcaat tccacagttt tcgcgatcca gactgaatgc ccacaggccg tcgagttttt
     4501 tgatttcacg ggttggggtt tctacaggac gtaacatcaa ttgtaattgt aaatagtaat
     4561 tgtaatgttg tttgttgttt gttgttgttg gtaattgttg taaaaatatg tcctctccaa
     4621 atgaaatgaa cttccttata tagaggaagg gtcttgcgaa ggatagtggg attgtgcgtc
     4681 atcccttacg tcagtggaga tgtcacatca atccacttgc tttgaagacg tggttggaac
     4741 gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc
     4801 agagagatct tgaatgatag cctttccttt atcgcaatga tggcatttgt aggagccacc
     4861 ttccttttct actgtccttt cgatgaagtg acagatagct gggcaatgga atccgaggag
     4921 gtttcccgaa attatccttt gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt
     4981 atctttgaca tttttggagt agaccagagt gtcgtgctcc accatgttac cggtcgtacg
     5041 aggcctatta atcccgatct agtaacatag atgacaccgc gcgcgataat ttatcctagt
     5101 ttgcgcgcta tattttgttt tctatcgcgt attaaatgta taattgcggg actctaatca
     5161 taaaaaccca tctcataaat aacgtcatgc attacatgtt aattattaca tgcttaacgt
     5221 aattcaacag aaattatatg ataatcatcg caagaccggc aacaggattc aatcttaaga
     5281 aactttattg ccaaatgttt gaacgatctc agatctcggt gacgggcagg accggacggg
     5341 gcggaaccgg caggctgaag tccagctgcc agaaacccac gtcatgccag ttcccgtgct
     5401 tgaagccggc cgcccgcagc atgccgcggg gggcatatcc gagcgcctcg tgcatgcgca
     5461 cgctcgggtc gttgggcagc ccgatgacag cgaccacgct cttgaagccc tgtgcctcca
     5521 gggacttcag caggtgggtg tagagcgtgg agcccagtcc cgtccgctgg tggcgggggg
     5581 agacgtacac ggtagactcg gccgtccagt cgtaggcgtt gcgtgccttc caggggccag
     5641 cgtaggcgat gccggcgacc tcgccgtcca cctcggcgac gagccaggga tagcgctccc
     5701 gcagacggac gaggtcgtcc gtccactcct gcggttcctg cggctcggta cggaagttga
     5761 ccgtgcttgt ctcgatgtag tggttgacga tggtgcagac cgccggcatg tccgcctcgg
     5821 tggcacggcg gatgtcggcc gggcgtcgtt ctgggctcat agatccggtg cagattattt
     5881 ggattgagag tgaatatgag actctaattg gataccgagg ggaatttatg gaacgtcagt
     5941 ggagcatttt tgacaagaaa tatttgctag ctgatagtga ccttaggcga cttttgaacg
     6001 cgcaataatg gtttctgacg tatgtgctta gctcattaaa ctccagaaac ccgcggctga
     6061 gtggctcctt caacgttgcg gttctgtcag ttccaaacgt aaaacggctt gtcccgcgtc
     6121 atcggcgggg gtcataacgt gactccctta attctccgct catgatcggc gcgccacgtg
     6181 acgcgtgttt acaccacaat atatcctgcc acgatttgcc catgggggtg ggcgaagaac
     6241 tccagcatga gatccccgcg ctggaggatc atccagccgg cgtcccggaa aacgattccg
     6301 aagcccaacc tttcatagaa ggcggcggtg gaatcgaaat ctcgtgatgg caggttgggc
     6361 gtcgcttggt cggtcatttc gaaccccaga gtcccgctca gaagaactcg tcaagaaggc
     6421 gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg aggaagcggt
     6481 cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct atgtcctgat
     6541 agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg ccattttcca
     6601 ccatgatatt cggcaagcag gcatcgccat gggtcacgac gagatcctcg ccgtcgggca
     6661 tgcgcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca
     6721 gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt
     6781 tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc cgcattgcat
     6841 cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga tcctgccccg
     6901 gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg agcacagctg
     6961 cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtcc tgaagttcat
     7021 tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc
     7081 ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc
     7141 tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg
     7201 atcctcatcc tgtctcttga tcagatcttg atcccctgcg ccatcagatc cttggcggca
     7261 agaaagccat ccagtttact ttgcagggct tcccaacctt accagagggc gccccagctg
     7321 gcaattccgg ttcgcttgct gtccataaaa ccgcccagtc tagctatcgc catgtaagcc
     7381 cactgcaagc tacctgcttt ctctttgcgc ttgcgttttc ccttgtccag atagcccagt
     7441 agctgacatt catcccag
//
