LOCUS       40924_47748        6847 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pYBA-1329, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6847)
  AUTHORS   Yao L, Yan X, Wang H, Cao M, Robaglia C.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-NOV-2014) Beijing Agro-Biotechnology Research Center, 
            Beijing Academy of Agriculture and Forestry Sciences, 9 Shuguang 
            Huayuan Zhonglu, Beijing, Beijing 100097, P.R. China
REFERENCE   2  (bases 1 to 6847)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6847)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (18-NOV-2014) Beijing Agro-Biotechnology Research Center, Beijing 
            Academy of Agriculture and Forestry Sciences, 9 Shuguang Huayuan 
            Zhonglu, Beijing, Beijing 100097, P.R. China"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6847
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      741..1510
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             1511..2170
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     misc_feature    2368..2391
                     /label=RB 'overdrive' sequence
                     /note="RB 'overdrive' sequence"
     misc_feature    complement(2392..2416)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    2428..2628
                     /note="CaMV poly-A signal; derived from pRT103"
     CDS             complement(2645..4294)
                     /label=luciferase
                     /note="firefly luciferase"
     misc_feature    complement(4295..4402)
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     terminator      complement(4445..4697)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4701..5249)
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
     promoter        complement(5258..5441)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(5576..5600)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(5790..6581)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag ctagcttgag
      601 tatactcact agactttgct tcgcaaagtc gtgaccgcct acggcggctg cggcgcccta
      661 cgggcttgct ctccgggctt cgccctgcgc ggtcgctgcg ctcccttgcc agcccgtgga
      721 tatgtggacg atggccgcga gcggccaccg gctggctcgc ttcgctcggc ccgtggacaa
      781 ccctgctgga caagctgatg gacaggctgc gcctgcccac gagcttgacc acagggattg
      841 cccaccggct acccagcctt cgaccacata cccaccggct ccaactgcgc ggcctgcggc
      901 cttgccccat caattttttt aattttctct ggggaaaagc ctccggcctg cggcctgcgc
      961 gcttcgcttg ccggttggac accaagtgga aggcgggtca aggctcgcgc agcgaccgcg
     1021 cagcggcttg gccttgacgc gcctggaacg acccaagcct atgcgagtgg gggcagtcga
     1081 aggcgaagcc cgcccgcctg ccccccgagc ctcacggcgg cgagtgcggg ggttccaagg
     1141 gggcagcgcc accttgggca aggccgaagg ccgcgcagtc gatcaacaag ccccggaggg
     1201 gccacttttt gccggagggg gagccgcgcc gaaggcgtgg gggaaccccg caggggtgcc
     1261 cttctttggg caccaaagaa ctagatatag ggcgaaatgc gaaagactta aaaatcaaca
     1321 acttaaaaaa ggggggtacg caacagctca ttgcggcacc ccccgcaata gctcattgcg
     1381 taggttaaag aaaatctgta attgactgcc acttttacgc aacgcataat tgttgtcgcg
     1441 ctgccgaaaa gttgcagctg attgcgcatg gtgccgcaac cgtgcggcac cctaccgcat
     1501 ggagataagc atggccacgc agtccagaga aatcggcatt caagccaaga acaagcccgg
     1561 tcactgggtg caaacggaac gcaaagcgca tgaggcgtgg gccgggctta ttgcgaggaa
     1621 acccacggcg gcaatgctgc tgcatcacct cgtggcgcag atgggccacc agaacgccgt
     1681 ggtggtcagc cagaagacac tttccaagct catcggacgt tctttgcgga cggtccaata
     1741 cgcagtcaag gacttggtgg ccgagcgctg gatctccgtc gtgaagctca acggccccgg
     1801 caccgtgtcg gcctacgtgg tcaatgaccg cgtggcgtgg ggccagcccc gcgaccagtt
     1861 gcgcctgtcg gtgttcagtg ccgccgtggt ggttgatcac gacgaccagg acgaatcgct
     1921 gttggggcat ggcgacctgc gccgcatccc gaccctgtat ccgggcgagc agcaactacc
     1981 gaccggcccc ggcgaggagc cgcccagcca gcccggcatt ccgggcatgg aaccagacct
     2041 gccagccttg accgaaacgg aggaatggga acggcgcggg cagcagcgcc tgccgatgcc
     2101 cgatgagccg tgttttctgg acgatggcga gccgttggag ccgccgacac gggtcacgct
     2161 gccgcgccgg tagcacttgg gttgcgcagc aacccgtaag tgcgctgttc cagactatcg
     2221 gctgtagccg cctcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat
     2281 ggagccgggc cacctcgacc tgaatggaag ccggcggcac ctcgctaacg gattcaccgt
     2341 ttttatcagg ctctgggagg cagaatacaa acaaacacat acagcgactt agtttacccg
     2401 ccaatatatc ctgtcaacgc gtttaaactg gattttggtt ttaggaatta gaaattttat
     2461 tgatagaagt attttacaaa tacaaataca tactaagggt ttcttatatg ctcaacacat
     2521 gagcgaaacc ctataagaac cctaattccc ttatctggga actactcaca cattattctg
     2581 gagaaaaata gagagagata gatttgtaga gagagactgg tgatttgcta gttagtcatc
     2641 gcgacacggc gatctttccg cccttcttgg cctttatgag gatctctctg atttttcttg
     2701 cgtcgagttt tccggtaaga cctttcggta cttcgtccac aaacacaact cctccgcgca
     2761 actttttcgc ggttgttact tgactggcga cgtaatccac gatctctttt tccgtcatcg
     2821 tctttccgtg ctccaaaaca acaacggcgg cgggaagttc accggcgtca tcgtcgggaa
     2881 gacctgcgac acctgcgtcg aagatgttgg ggtgttggag caagatggat tccaattcag
     2941 cgggagccac ctgatagcct ttgtacttaa tcagagactt caggcggtca acgatgaaga
     3001 agtgttcgtc ttcgtcccag taagctatgt ctccagaatg tagccatcca tccttgtcaa
     3061 tcaaggcgtt ggtcgcttcc ggattgttta cataaccgga cataatcata ggacctctca
     3121 cacacagttc gcctctttga ttaacgccca gcgttttccc ggtatccaga tccacaacct
     3181 tcgcttcaaa aaatggaaca actttaccga ccgcgcccgg tttatcatcc ccctcgggtg
     3241 taatcagaat agctgatgta gtctcagtga gcccatatcc ttgcctgata cctggcagat
     3301 ggaacctctt ggcaaccgct tccccgactt ccttagagag gggagcgcca ccagaagcaa
     3361 tttcgtgtaa attagataaa tcgtatttgt caatcagagt gcttttggcg aagaaggaga
     3421 atagggttgg caccagcagc gcactttgaa tcttgtaatc ctgaaggctc ctcagaaaca
     3481 gctcttcttc aaatctatac attaagacga ctcgaaatcc acatatcaaa tatccgagtg
     3541 tagtaaacat tccaaaaccg tgatggaatg gaacaacact taaaatcgca gtatccggaa
     3601 tgatttgatt gccaaaaata ggatctctgg catgcgagaa tctcacgcag gcagttctat
     3661 gaggcagagc gacaccttta ggcagaccag tagatccaga ggagttcatg atcagtgcaa
     3721 ttgtcttgtc cctatcgaag gactctggca caaaatcgta ttcattaaaa ccgggaggta
     3781 gatgagatgt gacgaacgtg tacatcgact gaaatccctg gtaatccgtt ttagaatcca
     3841 tgataataat tttttggatg attgggagct ttttttgcac gttcaaaatt ttttgcaacc
     3901 cctttttgga aacgaacacc acggtaggct gcgaaatgcc catactgttg agcaattcac
     3961 gttcattata aatgtcgttc gcgggcgcaa ctgcaactcc gataaataac gcgcccaaca
     4021 ccggcataaa gaattgaaga gagttttcac tgcatacgac gattctgtga tttgtattca
     4081 gcccatatcg tttcatagct tctgccaacc gaacggacat ttcgaagtac tcagcgtaag
     4141 tgatgtccac ctcgatatgt gcatctgtaa aagcaattgt tccaggaacc agggcgtatc
     4201 tcttcatagc cttatgcagt tgctctccag cggttccatc ttccagcgga tagaatggcg
     4261 ccgggccttt ctttatgttt ttggcgtctt ccatggtacc gggccccccc tcgaggtcga
     4321 cggtatcgat aagcttgata tcgaattcct gcagcccggg ggatccacta gttctagagc
     4381 ggccgccacc gcggtggagc tccaattggc cctatagacc ggtcgtacga ggcctattaa
     4441 tcccgatcta gtaacataga tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat
     4501 attttgtttt ctatcgcgta ttaaatgtat aattgcggga ctctaatcat aaaaacccat
     4561 ctcataaata acgtcatgca ttacatgtta attattacat gcttaacgta attcaacaga
     4621 aattatatga taatcatcgc aagaccggca acaggattca atcttaagaa actttattgc
     4681 caaatgtttg aacgatctca gatctcggtg acgggcagga ccggacgggg cggaaccggc
     4741 aggctgaagt ccagctgcca gaaacccacg tcatgccagt tcccgtgctt gaagccggcc
     4801 gcccgcagca tgccgcgggg ggcatatccg agcgcctcgt gcatgcgcac gctcgggtcg
     4861 ttgggcagcc cgatgacagc gaccacgctc ttgaagccct gtgcctccag ggacttcagc
     4921 aggtgggtgt agagcgtgga gcccagtccc gtccgctggt ggcgggggga gacgtacacg
     4981 gtagactcgg ccgtccagtc gtaggcgttg cgtgccttcc aggggccagc gtaggcgatg
     5041 ccggcgacct cgccgtccac ctcggcgacg agccagggat agcgctcccg cagacggacg
     5101 aggtcgtccg tccactcctg cggttcctgc ggctcggtac ggaagttgac cgtgcttgtc
     5161 tcgatgtagt ggttgacgat ggtgcagacc gccggcatgt ccgcctcggt ggcacggcgg
     5221 atgtcggccg ggcgtcgttc tgggctcata gatccggtgc agattatttg gattgagagt
     5281 gaatatgaga ctctaattgg ataccgaggg gaatttatgg aacgtcagtg gagcattttt
     5341 gacaagaaat atttgctagc tgatagtgac cttaggcgac ttttgaacgc gcaataatgg
     5401 tttctgacgt atgtgcttag ctcattaaac tccagaaacc cgcggctgag tggctccttc
     5461 aacgttgcgg ttctgtcagt tccaaacgta aaacggcttg tcccgcgtca tcggcggggg
     5521 tcataacgtg actcccttaa ttctccgctc atgatcggcg cgccacgtga cgcgtgttta
     5581 caccacaata tatcctgcca cgatttgccc atgggggtgg gcgaagaact ccagcatgag
     5641 atccccgcgc tggaggatca tccagccggc gtcccggaaa acgattccga agcccaacct
     5701 ttcatagaag gcggcggtgg aatcgaaatc tcgtgatggc aggttgggcg tcgcttggtc
     5761 ggtcatttcg aaccccagag tcccgctcag aagaactcgt caagaaggcg atagaaggcg
     5821 atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg
     5881 ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc
     5941 acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc
     6001 ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat gcgcgccttg
     6061 agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga
     6121 tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg
     6181 tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg
     6241 gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc
     6301 aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg
     6361 cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct gaagttcatt cagggcaccg
     6421 gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg
     6481 gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa
     6541 gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga tcctcatcct
     6601 gtctcttgat cagatcttga tcccctgcgc catcagatcc ttggcggcaa gaaagccatc
     6661 cagtttactt tgcagggctt cccaacctta ccagagggcg ccccagctgg caattccggt
     6721 tcgcttgctg tccataaaac cgcccagtct agctatcgcc atgtaagccc actgcaagct
     6781 acctgctttc tctttgcgct tgcgttttcc cttgtccaga tagcccagta gctgacattc
     6841 atcccag
//
