LOCUS       40924_47698        6080 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pYBA-1204, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6080)
  AUTHORS   Yao L, Yan X, Wang H, Cao M, Robaglia C.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-DEC-2015) Beijing Agro-Biotechnology Research Center, 
            Beijing Academy of Agriculture and Forestry Sciences, 9 Shuguang 
            Huayuan Zhonglu, Beijing 100097, P.R. China
REFERENCE   2  (bases 1 to 6080)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6080)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (02-DEC-2015) Beijing Agro-Biotechnology Research Center, Beijing 
            Academy of Agriculture and Forestry Sciences, 9 Shuguang Huayuan 
            Zhonglu, Beijing 100097, P.R. China"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6080
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      741..1510
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             1511..2170
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     misc_feature    2368..2391
                     /label=RB 'overdrive' sequence
                     /note="RB 'overdrive' sequence"
     misc_feature    complement(2392..2416)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     regulatory      2428..2628
                     /note="CaMV poly-A signal; derived from pRT103"
                     /regulatory_class="polyA_signal_sequence"
     misc_feature    complement(2645..2752)
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(2757..3102)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      complement(3204..3456)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3460..4482)
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     promoter        complement(4491..4674)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(4809..4833)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(5023..5814)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag ctagcttgag
      601 tatactcact agactttgct tcgcaaagtc gtgaccgcct acggcggctg cggcgcccta
      661 cgggcttgct ctccgggctt cgccctgcgc ggtcgctgcg ctcccttgcc agcccgtgga
      721 tatgtggacg atggccgcga gcggccaccg gctggctcgc ttcgctcggc ccgtggacaa
      781 ccctgctgga caagctgatg gacaggctgc gcctgcccac gagcttgacc acagggattg
      841 cccaccggct acccagcctt cgaccacata cccaccggct ccaactgcgc ggcctgcggc
      901 cttgccccat caattttttt aattttctct ggggaaaagc ctccggcctg cggcctgcgc
      961 gcttcgcttg ccggttggac accaagtgga aggcgggtca aggctcgcgc agcgaccgcg
     1021 cagcggcttg gccttgacgc gcctggaacg acccaagcct atgcgagtgg gggcagtcga
     1081 aggcgaagcc cgcccgcctg ccccccgagc ctcacggcgg cgagtgcggg ggttccaagg
     1141 gggcagcgcc accttgggca aggccgaagg ccgcgcagtc gatcaacaag ccccggaggg
     1201 gccacttttt gccggagggg gagccgcgcc gaaggcgtgg gggaaccccg caggggtgcc
     1261 cttctttggg caccaaagaa ctagatatag ggcgaaatgc gaaagactta aaaatcaaca
     1321 acttaaaaaa ggggggtacg caacagctca ttgcggcacc ccccgcaata gctcattgcg
     1381 taggttaaag aaaatctgta attgactgcc acttttacgc aacgcataat tgttgtcgcg
     1441 ctgccgaaaa gttgcagctg attgcgcatg gtgccgcaac cgtgcggcac cctaccgcat
     1501 ggagataagc atggccacgc agtccagaga aatcggcatt caagccaaga acaagcccgg
     1561 tcactgggtg caaacggaac gcaaagcgca tgaggcgtgg gccgggctta ttgcgaggaa
     1621 acccacggcg gcaatgctgc tgcatcacct cgtggcgcag atgggccacc agaacgccgt
     1681 ggtggtcagc cagaagacac tttccaagct catcggacgt tctttgcgga cggtccaata
     1741 cgcagtcaag gacttggtgg ccgagcgctg gatctccgtc gtgaagctca acggccccgg
     1801 caccgtgtcg gcctacgtgg tcaatgaccg cgtggcgtgg ggccagcccc gcgaccagtt
     1861 gcgcctgtcg gtgttcagtg ccgccgtggt ggttgatcac gacgaccagg acgaatcgct
     1921 gttggggcat ggcgacctgc gccgcatccc gaccctgtat ccgggcgagc agcaactacc
     1981 gaccggcccc ggcgaggagc cgcccagcca gcccggcatt ccgggcatgg aaccagacct
     2041 gccagccttg accgaaacgg aggaatggga acggcgcggg cagcagcgcc tgccgatgcc
     2101 cgatgagccg tgttttctgg acgatggcga gccgttggag ccgccgacac gggtcacgct
     2161 gccgcgccgg tagcacttgg gttgcgcagc aacccgtaag tgcgctgttc cagactatcg
     2221 gctgtagccg cctcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat
     2281 ggagccgggc cacctcgacc tgaatggaag ccggcggcac ctcgctaacg gattcaccgt
     2341 ttttatcagg ctctgggagg cagaatacaa acaaacacat acagcgactt agtttacccg
     2401 ccaatatatc ctgtcaacgc gtttaaactg gattttggtt ttaggaatta gaaattttat
     2461 tgatagaagt attttacaaa tacaaataca tactaagggt ttcttatatg ctcaacacat
     2521 gagcgaaacc ctataagaac cctaattccc ttatctggga actactcaca cattattctg
     2581 gagaaaaata gagagagata gatttgtaga gagagactgg tgatttgcta gttagtcatc
     2641 gcgaggtacc gggccccccc tcgaggtcga cggtatcgat aagcttgata tcgaattcct
     2701 gcagcccggg ggatccacta gttctagagc ggccgccacc gcggtggagc tccaattgtc
     2761 ctctccaaat gaaatgaact tccttatata gaggaagggt cttgcgaagg atagtgggat
     2821 tgtgcgtcat cccttacgtc agtggagatg tcacatcaat ccacttgctt tgaagacgtg
     2881 gttggaacgt cttctttttc cacgatgctc ctcgtgggtg ggggtccatc tttgggacca
     2941 ctgtcggcag agagatcttg aatgatagcc tttcctttat cgcaatgatg gcatttgtag
     3001 gagccacctt ccttttctac tgtcctttcg atgaagtgac agatagctgg gcaatggaat
     3061 ccgaggaggt ttcccgaaat tatcctttgt tgaaaagtct caatagccct ttggtcttct
     3121 gagactgtat ctttgacatt tttggagtag accagagtgt cgtgctccac catgttaccg
     3181 gtcgtacgag gcctattaat cccgatctag taacatagat gacaccgcgc gcgataattt
     3241 atcctagttt gcgcgctata ttttgttttc tatcgcgtat taaatgtata attgcgggac
     3301 tctaatcata aaaacccatc tcataaataa cgtcatgcat tacatgttaa ttattacatg
     3361 cttaacgtaa ttcaacagaa attatatgat aatcatcgca agaccggcaa caggattcaa
     3421 tcttaagaaa ctttattgcc aaatgtttga acgatcctat ttctttgccc tcggacgagt
     3481 gctggggcgt cggtttccac tatcggcgag tacttctaca cagccatcgg tccagacggc
     3541 cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg gctccggatc ggacgattgc
     3601 gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca agctctgata
     3661 gagttggtca agaccaatgc ggagcatata cgcccggagt cgtggcgatc ctgcaagctc
     3721 cggatgcctc cgctcgaagt agcgcgtctg ctgctccata caagccaacc acggcctcca
     3781 gaagaagatg ttggcgacct cgtattggga atccccgaac atcgcctcgc tccagtcaat
     3841 gaccgctgtt atgcggccat tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac
     3901 gaggtgccgg acttcggggc agtcctcggc ccaaagcatc agctcatcga gagcctgcgc
     3961 gacggacgca ctgacggtgt cgtccatcac agtttgccag tgatacacat ggggatcagc
     4021 aatcgcgcat atgaaatcac gccatgtagt gtattgaccg attccttgcg gtccgaatgg
     4081 gccgaacccg ctcgtctggc taagatcggc cgcagcgatc gcatccatag cctccgcgac
     4141 tggttgtaga acagcgggca gttcggtttc aggcaggtct tgcaacgtga caccctgtgc
     4201 acggcgggag atgcaatagg tcaggctctc gctaaactcc ccaatgtcaa gcacttccgg
     4261 aatcgggagc gcggccgatg caaagtgccg ataaacataa cgatctttgt agaaaccatc
     4321 ggcgcagcta tttacccgca ggacatatcc acgccctcct acatcgaagc tgaaagcacg
     4381 agattcttcg ccctccgaga gctgcatcag gtcggagacg ctgtcgaact tttcgatcag
     4441 aaacttctcg acagacgtcg cggtgagttc aggctttttc atagatccgg tgcagattat
     4501 ttggattgag agtgaatatg agactctaat tggataccga ggggaattta tggaacgtca
     4561 gtggagcatt tttgacaaga aatatttgct agctgatagt gaccttaggc gacttttgaa
     4621 cgcgcaataa tggtttctga cgtatgtgct tagctcatta aactccagaa acccgcggct
     4681 gagtggctcc ttcaacgttg cggttctgtc agttccaaac gtaaaacggc ttgtcccgcg
     4741 tcatcggcgg gggtcataac gtgactccct taattctccg ctcatgatcg gcgcgccacg
     4801 tgacgcgtgt ttacaccaca atatatcctg ccacgatttg cccatggggg tgggcgaaga
     4861 actccagcat gagatccccg cgctggagga tcatccagcc ggcgtcccgg aaaacgattc
     4921 cgaagcccaa cctttcatag aaggcggcgg tggaatcgaa atctcgtgat ggcaggttgg
     4981 gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct cagaagaact cgtcaagaag
     5041 gcgatagaag gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg
     5101 gtcagcccat tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg
     5161 atagcggtcc gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc
     5221 caccatgata ttcggcaagc aggcatcgcc atgggtcacg acgagatcct cgccgtcggg
     5281 catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc
     5341 cagatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg
     5401 tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc
     5461 atcagccatg atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc
     5521 cggcacttcg cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc
     5581 tgcgcaagga acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cctgaagttc
     5641 attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag
     5701 ccggaacacg gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag
     5761 cctctccacc caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa
     5821 cgatcctcat cctgtctctt gatcagatct tgatcccctg cgccatcaga tccttggcgg
     5881 caagaaagcc atccagttta ctttgcaggg cttcccaacc ttaccagagg gcgccccagc
     5941 tggcaattcc ggttcgcttg ctgtccataa aaccgcccag tctagctatc gccatgtaag
     6001 cccactgcaa gctacctgct ttctctttgc gcttgcgttt tcccttgtcc agatagccca
     6061 gtagctgaca ttcatcccag
//
