LOCUS       40924_47678        7548 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pYBA-1159, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7548)
  AUTHORS   Yao L, Yan X, Wang H, Cao M, Robaglia C.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2014) Beijing Agro-Biotechnology Research Center, 
            Beijing Academy of Agriculture and Forestry Sciences, 9 Shuguang 
            Huayuan Zhonglu, Beijing, Beijing 100097, P.R. China
REFERENCE   2  (bases 1 to 7548)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7548)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (02-NOV-2014) Beijing Agro-Biotechnology Research Center, Beijing 
            Academy of Agriculture and Forestry Sciences, 9 Shuguang Huayuan 
            Zhonglu, Beijing, Beijing 100097, P.R. China"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7548
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      741..1510
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             1511..2170
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     misc_feature    2368..2391
                     /label=RB overdrive sequence
                     /note="RB overdrive sequence"
     misc_feature    complement(2392..2416)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    2428..2628
                     /note="CaMV poly-A signal; derived from pRT103"
     primer_bind     2662..2678
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2712..2728)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(2729..4378)
                     /label=luciferase
                     /note="firefly luciferase"
     misc_feature    complement(4390..4445)
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     promoter        complement(4456..4801)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      complement(4903..5155)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(5159..5950)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(5959..6142)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(6277..6301)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(6491..7282)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag ctagcttgag
      601 tatactcact agactttgct tcgcaaagtc gtgaccgcct acggcggctg cggcgcccta
      661 cgggcttgct ctccgggctt cgccctgcgc ggtcgctgcg ctcccttgcc agcccgtgga
      721 tatgtggacg atggccgcga gcggccaccg gctggctcgc ttcgctcggc ccgtggacaa
      781 ccctgctgga caagctgatg gacaggctgc gcctgcccac gagcttgacc acagggattg
      841 cccaccggct acccagcctt cgaccacata cccaccggct ccaactgcgc ggcctgcggc
      901 cttgccccat caattttttt aattttctct ggggaaaagc ctccggcctg cggcctgcgc
      961 gcttcgcttg ccggttggac accaagtgga aggcgggtca aggctcgcgc agcgaccgcg
     1021 cagcggcttg gccttgacgc gcctggaacg acccaagcct atgcgagtgg gggcagtcga
     1081 aggcgaagcc cgcccgcctg ccccccgagc ctcacggcgg cgagtgcggg ggttccaagg
     1141 gggcagcgcc accttgggca aggccgaagg ccgcgcagtc gatcaacaag ccccggaggg
     1201 gccacttttt gccggagggg gagccgcgcc gaaggcgtgg gggaaccccg caggggtgcc
     1261 cttctttggg caccaaagaa ctagatatag ggcgaaatgc gaaagactta aaaatcaaca
     1321 acttaaaaaa ggggggtacg caacagctca ttgcggcacc ccccgcaata gctcattgcg
     1381 taggttaaag aaaatctgta attgactgcc acttttacgc aacgcataat tgttgtcgcg
     1441 ctgccgaaaa gttgcagctg attgcgcatg gtgccgcaac cgtgcggcac cctaccgcat
     1501 ggagataagc atggccacgc agtccagaga aatcggcatt caagccaaga acaagcccgg
     1561 tcactgggtg caaacggaac gcaaagcgca tgaggcgtgg gccgggctta ttgcgaggaa
     1621 acccacggcg gcaatgctgc tgcatcacct cgtggcgcag atgggccacc agaacgccgt
     1681 ggtggtcagc cagaagacac tttccaagct catcggacgt tctttgcgga cggtccaata
     1741 cgcagtcaag gacttggtgg ccgagcgctg gatctccgtc gtgaagctca acggccccgg
     1801 caccgtgtcg gcctacgtgg tcaatgaccg cgtggcgtgg ggccagcccc gcgaccagtt
     1861 gcgcctgtcg gtgttcagtg ccgccgtggt ggttgatcac gacgaccagg acgaatcgct
     1921 gttggggcat ggcgacctgc gccgcatccc gaccctgtat ccgggcgagc agcaactacc
     1981 gaccggcccc ggcgaggagc cgcccagcca gcccggcatt ccgggcatgg aaccagacct
     2041 gccagccttg accgaaacgg aggaatggga acggcgcggg cagcagcgcc tgccgatgcc
     2101 cgatgagccg tgttttctgg acgatggcga gccgttggag ccgccgacac gggtcacgct
     2161 gccgcgccgg tagcacttgg gttgcgcagc aacccgtaag tgcgctgttc cagactatcg
     2221 gctgtagccg cctcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat
     2281 ggagccgggc cacctcgacc tgaatggaag ccggcggcac ctcgctaacg gattcaccgt
     2341 ttttatcagg ctctgggagg cagaatacaa acaaacacat acagcgactt agtttacccg
     2401 ccaatatatc ctgtcaacgc gtttaaactg gattttggtt ttaggaatta gaaattttat
     2461 tgatagaagt attttacaaa tacaaataca tactaagggt ttcttatatg ctcaacacat
     2521 gagcgaaacc ctataagaac cctaattccc ttatctggga actactcaca cattattctg
     2581 gagaaaaata gagagagata gatttgtaga gagagactgg tgatttgcta gttagtcatc
     2641 gcgaggtacc gggccccccc tcgaggtcga cggtatcgat aagcttgata tcgaattcct
     2701 gcagcccggg ggatccacta gttctagaca cggcgatctt tccgcccttc ttggccttta
     2761 tgaggatctc tctgattttt cttgcgtcga gttttccggt aagacctttc ggtacttcgt
     2821 ccacaaacac aactcctccg cgcaactttt tcgcggttgt tacttgactg gcgacgtaat
     2881 ccacgatctc tttttccgtc atcgtctttc cgtgctccaa aacaacaacg gcggcgggaa
     2941 gttcaccggc gtcatcgtcg ggaagacctg cgacacctgc gtcgaagatg ttggggtgtt
     3001 ggagcaagat ggattccaat tcagcgggag ccacctgata gcctttgtac ttaatcagag
     3061 acttcaggcg gtcaacgatg aagaagtgtt cgtcttcgtc ccagtaagct atgtctccag
     3121 aatgtagcca tccatccttg tcaatcaagg cgttggtcgc ttccggattg tttacataac
     3181 cggacataat cataggacct ctcacacaca gttcgcctct ttgattaacg cccagcgttt
     3241 tcccggtatc cagatccaca accttcgctt caaaaaatgg aacaacttta ccgaccgcgc
     3301 ccggtttatc atccccctcg ggtgtaatca gaatagctga tgtagtctca gtgagcccat
     3361 atccttgcct gatacctggc agatggaacc tcttggcaac cgcttccccg acttccttag
     3421 agaggggagc gccaccagaa gcaatttcgt gtaaattaga taaatcgtat ttgtcaatca
     3481 gagtgctttt ggcgaagaag gagaataggg ttggcaccag cagcgcactt tgaatcttgt
     3541 aatcctgaag gctcctcaga aacagctctt cttcaaatct atacattaag acgactcgaa
     3601 atccacatat caaatatccg agtgtagtaa acattccaaa accgtgatgg aatggaacaa
     3661 cacttaaaat cgcagtatcc ggaatgattt gattgccaaa aataggatct ctggcatgcg
     3721 agaatctcac gcaggcagtt ctatgaggca gagcgacacc tttaggcaga ccagtagatc
     3781 cagaggagtt catgatcagt gcaattgtct tgtccctatc gaaggactct ggcacaaaat
     3841 cgtattcatt aaaaccggga ggtagatgag atgtgacgaa cgtgtacatc gactgaaatc
     3901 cctggtaatc cgttttagaa tccatgataa taattttttg gatgattggg agcttttttt
     3961 gcacgttcaa aattttttgc aacccctttt tggaaacgaa caccacggta ggctgcgaaa
     4021 tgcccatact gttgagcaat tcacgttcat tataaatgtc gttcgcgggc gcaactgcaa
     4081 ctccgataaa taacgcgccc aacaccggca taaagaattg aagagagttt tcactgcata
     4141 cgacgattct gtgatttgta ttcagcccat atcgtttcat agcttctgcc aaccgaacgg
     4201 acatttcgaa gtactcagcg taagtgatgt ccacctcgat atgtgcatct gtaaaagcaa
     4261 ttgttccagg aaccagggcg tatctcttca tagccttatg cagttgctct ccagcggttc
     4321 catcttccag cggatagaat ggcgccgggc ctttctttat gtttttggcg tcttccatga
     4381 gctccaattg taattgtaaa tagtaattgt aatgttgttt gttgtttgtt gttgttggta
     4441 attgttgtaa aaatatgtcc tctccaaatg aaatgaactt ccttatatag aggaagggtc
     4501 ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatgt cacatcaatc
     4561 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg
     4621 gggtccatct ttgggaccac tgtcggcaga gagatcttga atgatagcct ttcctttatc
     4681 gcaatgatgg catttgtagg agccaccttc cttttctact gtcctttcga tgaagtgaca
     4741 gatagctggg caatggaatc cgaggaggtt tcccgaaatt atcctttgtt gaaaagtctc
     4801 aatagccctt tggtcttctg agactgtatc tttgacattt ttggagtaga ccagagtgtc
     4861 gtgctccacc atgttaccgg tcgtacgagg cctattaatc ccgatctagt aacatagatg
     4921 acaccgcgcg cgataattta tcctagtttg cgcgctatat tttgttttct atcgcgtatt
     4981 aaatgtataa ttgcgggact ctaatcataa aaacccatct cataaataac gtcatgcatt
     5041 acatgttaat tattacatgc ttaacgtaat tcaacagaaa ttatatgata atcatcgcaa
     5101 gaccggcaac aggattcaat cttaagaaac tttattgcca aatgtttgaa cgatctcaga
     5161 agaactcgtc aagaaggcga tagaaggcga tgcgctgcga atcgggagcg gcgataccgt
     5221 aaagcacgag gaagcggtca gcccattcgc cgccaagctc ttcagcaata tcacgggtag
     5281 ccaacgctat gtcctgatag cggtccgcca cacccagccg gccacagtcg atgaatccag
     5341 aaaagcggcc attttccacc atgatattcg gcaagcaggc atcgccatgg gtcacgacga
     5401 gatcctcgcc gtcgggcatg cgcgccttga gcctggcgaa cagttcggct ggcgcgagcc
     5461 cctgatgctc ttcgtccaga tcatcctgat cgacaagacc ggcttccatc cgagtacgtg
     5521 ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca ggtagccgga tcaagcgtat
     5581 gcagccgccg cattgcatca gccatgatgg atactttctc ggcaggagca aggtgagatg
     5641 acaggagatc ctgccccggc acttcgccca atagcagcca gtcccttccc gcttcagtga
     5701 caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc cagccacgat agccgcgctg
     5761 cctcgtcctg aagttcattc agggcaccgg acaggtcggt cttgacaaaa agaaccgggc
     5821 gcccctgcgc tgacagccgg aacacggcgg catcagagca gccgattgtc tgttgtgccc
     5881 agtcatagcc gaatagcctc tccacccaag cggccggaga acctgcgtgc aatccatctt
     5941 gttcaatcat agatccggtg cagattattt ggattgagag tgaatatgag actctaattg
     6001 gataccgagg ggaatttatg gaacgtcagt ggagcatttt tgacaagaaa tatttgctag
     6061 ctgatagtga ccttaggcga cttttgaacg cgcaataatg gtttctgacg tatgtgctta
     6121 gctcattaaa ctccagaaac ccgcggctga gtggctcctt caacgttgcg gttctgtcag
     6181 ttccaaacgt aaaacggctt gtcccgcgtc atcggcgggg gtcataacgt gactccctta
     6241 attctccgct catgatcggc gcgccacgtg acgcgtgttt acaccacaat atatcctgcc
     6301 acgatttgcc catgggggtg ggcgaagaac tccagcatga gatccccgcg ctggaggatc
     6361 atccagccgg cgtcccggaa aacgattccg aagcccaacc tttcatagaa ggcggcggtg
     6421 gaatcgaaat ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc gaaccccaga
     6481 gtcccgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag
     6541 cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc tcttcagcaa
     6601 tatcacgggt agccaacgct atgtcctgat agcggtccgc cacacccagc cggccacagt
     6661 cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag gcatcgccat
     6721 gggtcacgac gagatcctcg ccgtcgggca tgcgcgcctt gagcctggcg aacagttcgg
     6781 ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga ccggcttcca
     6841 tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg
     6901 gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc tcggcaggag
     6961 caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc cagtcccttc
     7021 ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg gccagccacg
     7081 atagccgcgc tgcctcgtcc tgaagttcat tcagggcacc ggacaggtcg gtcttgacaa
     7141 aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag cagccgattg
     7201 tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga gaacctgcgt
     7261 gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga tcagatcttg
     7321 atcccctgcg ccatcagatc cttggcggca agaaagccat ccagtttact ttgcagggct
     7381 tcccaacctt accagagggc gccccagctg gcaattccgg ttcgcttgct gtccataaaa
     7441 ccgcccagtc tagctatcgc catgtaagcc cactgcaagc tacctgcttt ctctttgcgc
     7501 ttgcgttttc ccttgtccag atagcccagt agctgacatt catcccag
//
