LOCUS       40924_47668        6585 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pYBA-1157, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6585)
  AUTHORS   Yao L, Yan X, Wang H, Cao M, Robaglia C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-NOV-2013) Beijing Agro-Biotechnology Research Center, 
            Beijing Academy of Agriculture and Forestry Sciences, 9 Shuguang 
            Huayuan Zhonglu, Beijing, Beijing 100097, P.R. China
REFERENCE   2  (bases 1 to 6585)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6585)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (19-NOV-2013) Beijing Agro-Biotechnology Research Center, Beijing 
            Academy of Agriculture and Forestry Sciences, 9 Shuguang Huayuan 
            Zhonglu, Beijing, Beijing 100097, P.R. China"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6585
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      741..1510
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             1511..2170
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     misc_feature    2368..2391
                     /label=RB 'overdrive' sequence
                     /note="RB 'overdrive' sequence"
     misc_feature    complement(2392..2416)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    2428..2628
                     /note="CaMV poly-A signal; derived from pRT103"
     primer_bind     2662..2678
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2712..2728)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(2729..3415)
                     /label=TagRFP
                     /note="monomeric derivative of red fluorescent protein from
                     Entacmaea quadricolor (Merzlyak et al., 2007)"
     misc_feature    complement(3427..3482)
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     promoter        complement(3493..3838)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     regulatory      3912..3943
                     /label=CaMV 35S promoter
                     /note="CaMV 35S promoter"
                     /regulatory_class="promoter"
     terminator      complement(3940..4192)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4196..4987)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(4996..5179)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(5314..5338)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(5528..6319)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag ctagcttgag
      601 tatactcact agactttgct tcgcaaagtc gtgaccgcct acggcggctg cggcgcccta
      661 cgggcttgct ctccgggctt cgccctgcgc ggtcgctgcg ctcccttgcc agcccgtgga
      721 tatgtggacg atggccgcga gcggccaccg gctggctcgc ttcgctcggc ccgtggacaa
      781 ccctgctgga caagctgatg gacaggctgc gcctgcccac gagcttgacc acagggattg
      841 cccaccggct acccagcctt cgaccacata cccaccggct ccaactgcgc ggcctgcggc
      901 cttgccccat caattttttt aattttctct ggggaaaagc ctccggcctg cggcctgcgc
      961 gcttcgcttg ccggttggac accaagtgga aggcgggtca aggctcgcgc agcgaccgcg
     1021 cagcggcttg gccttgacgc gcctggaacg acccaagcct atgcgagtgg gggcagtcga
     1081 aggcgaagcc cgcccgcctg ccccccgagc ctcacggcgg cgagtgcggg ggttccaagg
     1141 gggcagcgcc accttgggca aggccgaagg ccgcgcagtc gatcaacaag ccccggaggg
     1201 gccacttttt gccggagggg gagccgcgcc gaaggcgtgg gggaaccccg caggggtgcc
     1261 cttctttggg caccaaagaa ctagatatag ggcgaaatgc gaaagactta aaaatcaaca
     1321 acttaaaaaa ggggggtacg caacagctca ttgcggcacc ccccgcaata gctcattgcg
     1381 taggttaaag aaaatctgta attgactgcc acttttacgc aacgcataat tgttgtcgcg
     1441 ctgccgaaaa gttgcagctg attgcgcatg gtgccgcaac cgtgcggcac cctaccgcat
     1501 ggagataagc atggccacgc agtccagaga aatcggcatt caagccaaga acaagcccgg
     1561 tcactgggtg caaacggaac gcaaagcgca tgaggcgtgg gccgggctta ttgcgaggaa
     1621 acccacggcg gcaatgctgc tgcatcacct cgtggcgcag atgggccacc agaacgccgt
     1681 ggtggtcagc cagaagacac tttccaagct catcggacgt tctttgcgga cggtccaata
     1741 cgcagtcaag gacttggtgg ccgagcgctg gatctccgtc gtgaagctca acggccccgg
     1801 caccgtgtcg gcctacgtgg tcaatgaccg cgtggcgtgg ggccagcccc gcgaccagtt
     1861 gcgcctgtcg gtgttcagtg ccgccgtggt ggttgatcac gacgaccagg acgaatcgct
     1921 gttggggcat ggcgacctgc gccgcatccc gaccctgtat ccgggcgagc agcaactacc
     1981 gaccggcccc ggcgaggagc cgcccagcca gcccggcatt ccgggcatgg aaccagacct
     2041 gccagccttg accgaaacgg aggaatggga acggcgcggg cagcagcgcc tgccgatgcc
     2101 cgatgagccg tgttttctgg acgatggcga gccgttggag ccgccgacac gggtcacgct
     2161 gccgcgccgg tagcacttgg gttgcgcagc aacccgtaag tgcgctgttc cagactatcg
     2221 gctgtagccg cctcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat
     2281 ggagccgggc cacctcgacc tgaatggaag ccggcggcac ctcgctaacg gattcaccgt
     2341 ttttatcagg ctctgggagg cagaatacaa acaaacacat acagcgactt agtttacccg
     2401 ccaatatatc ctgtcaacgc gtttaaactg gattttggtt ttaggaatta gaaattttat
     2461 tgatagaagt attttacaaa tacaaataca tactaagggt ttcttatatg ctcaacacat
     2521 gagcgaaacc ctataagaac cctaattccc ttatctggga actactcaca cattattctg
     2581 gagaaaaata gagagagata gatttgtaga gagagactgg tgatttgcta gttagtcatc
     2641 gcgaggtacc gggccccccc tcgaggtcga cggtatcgat aagcttgata tcgaattcct
     2701 gcagcccggg ggatccacta gttctagact tgtgccccag tttgctaggg aggtcgcagt
     2761 atctggccac agccacctcg tgctgctcga cgtaggtctc tttgtcggcc tccttgattc
     2821 tttccagtct gtggtccaca tagtagacgc cgggcatctt gaggttctta gcgggtttct
     2881 tggatctgta tgtggtcttg aagttgcaga tcaggtggcc cccgcccacg agcttcaggg
     2941 ccatgtcgct tctgccttcc aggccgccgt cagcggggta cagcatctcg gtgttggcct
     3001 cccagccgag tgttttcttc tgcatcacag ggccgttgga tgggaagttc acccctctga
     3061 tcttgacgtt gtagatgagg cagccgtcct ggaggctggt gtcctgggta gcggtcagca
     3121 cgcccccgtc ttcgtatgtg gtgactctct cccatgtgaa gccctcaggg aaggactgct
     3181 taaagaagtc ggggatgccc tgggtgtggt tgatgaaggt tctgctgccg tacatgaagc
     3241 tggtagccag gatgtcgaag gcgaagggga gagggccgcc ctcgaccacc ttgattctca
     3301 tggtctgggt gccctcgtag ggcttgcctt cgccctcgga tgtgcacttg aagtggtggt
     3361 tgttcacggt gccctccatg tacagcttca tgtgcatgtt ctccttaatc agctcgctca
     3421 tcaattgtaa ttgtaaatag taattgtaat gttgtttgtt gtttgttgtt gttggtaatt
     3481 gttgtaaaaa tatgtcctct ccaaatgaaa tgaacttcct tatatagagg aagggtcttg
     3541 cgaaggatag tgggattgtg cgtcatccct tacgtcagtg gagatgtcac atcaatccac
     3601 ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg tgggtggggg
     3661 tccatctttg ggaccactgt cggcagagag atcttgaatg atagcctttc ctttatcgca
     3721 atgatggcat ttgtaggagc caccttcctt ttctactgtc ctttcgatga agtgacagat
     3781 agctgggcaa tggaatccga ggaggtttcc cgaaattatc ctttgttgaa aagtctcaat
     3841 agccctttgg tcttctgaga ctgtatcttt gacatttttg gagtagacca gagtgtcgtg
     3901 ctccaccatg ttaccggtcg tacgaggcct attaatcccg atctagtaac atagatgaca
     3961 ccgcgcgcga taatttatcc tagtttgcgc gctatatttt gttttctatc gcgtattaaa
     4021 tgtataattg cgggactcta atcataaaaa cccatctcat aaataacgtc atgcattaca
     4081 tgttaattat tacatgctta acgtaattca acagaaatta tatgataatc atcgcaagac
     4141 cggcaacagg attcaatctt aagaaacttt attgccaaat gtttgaacga tctcagaaga
     4201 actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa
     4261 gcacgaggaa gcggtcagcc cattcgccgc caagctcttc agcaatatca cgggtagcca
     4321 acgctatgtc ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa
     4381 agcggccatt ttccaccatg atattcggca agcaggcatc gccatgggtc acgacgagat
     4441 cctcgccgtc gggcatgcgc gccttgagcc tggcgaacag ttcggctggc gcgagcccct
     4501 gatgctcttc gtccagatca tcctgatcga caagaccggc ttccatccga gtacgtgctc
     4561 gctcgatgcg atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca
     4621 gccgccgcat tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca
     4681 ggagatcctg ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa
     4741 cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct
     4801 cgtcctgaag ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc
     4861 cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt
     4921 catagccgaa tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt
     4981 caatcataga tccggtgcag attatttgga ttgagagtga atatgagact ctaattggat
     5041 accgagggga atttatggaa cgtcagtgga gcatttttga caagaaatat ttgctagctg
     5101 atagtgacct taggcgactt ttgaacgcgc aataatggtt tctgacgtat gtgcttagct
     5161 cattaaactc cagaaacccg cggctgagtg gctccttcaa cgttgcggtt ctgtcagttc
     5221 caaacgtaaa acggcttgtc ccgcgtcatc ggcgggggtc ataacgtgac tcccttaatt
     5281 ctccgctcat gatcggcgcg ccacgtgacg cgtgtttaca ccacaatata tcctgccacg
     5341 atttgcccat gggggtgggc gaagaactcc agcatgagat ccccgcgctg gaggatcatc
     5401 cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc ggcggtggaa
     5461 tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa ccccagagtc
     5521 ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg
     5581 cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct tcagcaatat
     5641 cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg ccacagtcga
     5701 tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca tcgccatggg
     5761 tcacgacgag atcctcgccg tcgggcatgc gcgccttgag cctggcgaac agttcggctg
     5821 gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg gcttccatcc
     5881 gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag gtagccggat
     5941 caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg gcaggagcaa
     6001 ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag tcccttcccg
     6061 cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc agccacgata
     6121 gccgcgctgc ctcgtcctga agttcattca gggcaccgga caggtcggtc ttgacaaaaa
     6181 gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag ccgattgtct
     6241 gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa cctgcgtgca
     6301 atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca gatcttgatc
     6361 ccctgcgcca tcagatcctt ggcggcaaga aagccatcca gtttactttg cagggcttcc
     6421 caaccttacc agagggcgcc ccagctggca attccggttc gcttgctgtc cataaaaccg
     6481 cccagtctag ctatcgccat gtaagcccac tgcaagctac ctgctttctc tttgcgcttg
     6541 cgttttccct tgtccagata gcccagtagc tgacattcat cccag
//
