LOCUS       40924_47623        6542 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pYBA-1137, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6542)
  AUTHORS   Yao L, Yan X, Wang H, Cao M, Robaglia C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-NOV-2013) Beijing Agro-Biotechnology Research Center, 
            Beijing Academy of Agriculture and Forestry Sciences, 9 Shuguang 
            Huayuan Zhonglu, Beijing, Beijing 100097, P.R. China
REFERENCE   2  (bases 1 to 6542)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6542)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (19-NOV-2013) Beijing Agro-Biotechnology Research Center, Beijing 
            Academy of Agriculture and Forestry Sciences, 9 Shuguang Huayuan 
            Zhonglu, Beijing, Beijing 100097, P.R. China"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6542
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      741..1510
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             1511..2170
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     misc_feature    2368..2391
                     /label=RB 'overdrive' sequence
                     /note="RB 'overdrive' sequence"
     misc_feature    complement(2392..2416)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    2428..2628
                     /note="CaMV poly-A signal; derived from pRT103"
     CDS             complement(2645..3331)
                     /label=TagRFP
                     /note="monomeric derivative of red fluorescent protein from
                     Entacmaea quadricolor (Merzlyak et al., 2007)"
     misc_feature    complement(3338..3445)
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(3450..3795)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      complement(3897..4149)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4153..4944)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(4953..5136)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(5271..5295)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(5485..6276)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag ctagcttgag
      601 tatactcact agactttgct tcgcaaagtc gtgaccgcct acggcggctg cggcgcccta
      661 cgggcttgct ctccgggctt cgccctgcgc ggtcgctgcg ctcccttgcc agcccgtgga
      721 tatgtggacg atggccgcga gcggccaccg gctggctcgc ttcgctcggc ccgtggacaa
      781 ccctgctgga caagctgatg gacaggctgc gcctgcccac gagcttgacc acagggattg
      841 cccaccggct acccagcctt cgaccacata cccaccggct ccaactgcgc ggcctgcggc
      901 cttgccccat caattttttt aattttctct ggggaaaagc ctccggcctg cggcctgcgc
      961 gcttcgcttg ccggttggac accaagtgga aggcgggtca aggctcgcgc agcgaccgcg
     1021 cagcggcttg gccttgacgc gcctggaacg acccaagcct atgcgagtgg gggcagtcga
     1081 aggcgaagcc cgcccgcctg ccccccgagc ctcacggcgg cgagtgcggg ggttccaagg
     1141 gggcagcgcc accttgggca aggccgaagg ccgcgcagtc gatcaacaag ccccggaggg
     1201 gccacttttt gccggagggg gagccgcgcc gaaggcgtgg gggaaccccg caggggtgcc
     1261 cttctttggg caccaaagaa ctagatatag ggcgaaatgc gaaagactta aaaatcaaca
     1321 acttaaaaaa ggggggtacg caacagctca ttgcggcacc ccccgcaata gctcattgcg
     1381 taggttaaag aaaatctgta attgactgcc acttttacgc aacgcataat tgttgtcgcg
     1441 ctgccgaaaa gttgcagctg attgcgcatg gtgccgcaac cgtgcggcac cctaccgcat
     1501 ggagataagc atggccacgc agtccagaga aatcggcatt caagccaaga acaagcccgg
     1561 tcactgggtg caaacggaac gcaaagcgca tgaggcgtgg gccgggctta ttgcgaggaa
     1621 acccacggcg gcaatgctgc tgcatcacct cgtggcgcag atgggccacc agaacgccgt
     1681 ggtggtcagc cagaagacac tttccaagct catcggacgt tctttgcgga cggtccaata
     1741 cgcagtcaag gacttggtgg ccgagcgctg gatctccgtc gtgaagctca acggccccgg
     1801 caccgtgtcg gcctacgtgg tcaatgaccg cgtggcgtgg ggccagcccc gcgaccagtt
     1861 gcgcctgtcg gtgttcagtg ccgccgtggt ggttgatcac gacgaccagg acgaatcgct
     1921 gttggggcat ggcgacctgc gccgcatccc gaccctgtat ccgggcgagc agcaactacc
     1981 gaccggcccc ggcgaggagc cgcccagcca gcccggcatt ccgggcatgg aaccagacct
     2041 gccagccttg accgaaacgg aggaatggga acggcgcggg cagcagcgcc tgccgatgcc
     2101 cgatgagccg tgttttctgg acgatggcga gccgttggag ccgccgacac gggtcacgct
     2161 gccgcgccgg tagcacttgg gttgcgcagc aacccgtaag tgcgctgttc cagactatcg
     2221 gctgtagccg cctcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat
     2281 ggagccgggc cacctcgacc tgaatggaag ccggcggcac ctcgctaacg gattcaccgt
     2341 ttttatcagg ctctgggagg cagaatacaa acaaacacat acagcgactt agtttacccg
     2401 ccaatatatc ctgtcaacgc gtttaaactg gattttggtt ttaggaatta gaaattttat
     2461 tgatagaagt attttacaaa tacaaataca tactaagggt ttcttatatg ctcaacacat
     2521 gagcgaaacc ctataagaac cctaattccc ttatctggga actactcaca cattattctg
     2581 gagaaaaata gagagagata gatttgtaga gagagactgg tgatttgcta gttagtcatc
     2641 gcgacttgtg ccccagtttg ctagggaggt cgcagtatct ggccacagcc acctcgtgct
     2701 gctcgacgta ggtctctttg tcggcctcct tgattctttc cagtctgtgg tccacatagt
     2761 agacgccggg catcttgagg ttcttagcgg gtttcttgga tctgtatgtg gtcttgaagt
     2821 tgcagatcag gtggcccccg cccacgagct tcagggccat gtcgcttctg ccttccaggc
     2881 cgccgtcagc ggggtacagc atctcggtgt tggcctccca gccgagtgtt ttcttctgca
     2941 tcacagggcc gttggatggg aagttcaccc ctctgatctt gacgttgtag atgaggcagc
     3001 cgtcctggag gctggtgtcc tgggtagcgg tcagcacgcc cccgtcttcg tatgtggtga
     3061 ctctctccca tgtgaagccc tcagggaagg actgcttaaa gaagtcgggg atgccctggg
     3121 tgtggttgat gaaggttctg ctgccgtaca tgaagctggt agccaggatg tcgaaggcga
     3181 aggggagagg gccgccctcg accaccttga ttctcatggt ctgggtgccc tcgtagggct
     3241 tgccttcgcc ctcggatgtg cacttgaagt ggtggttgtt cacggtgccc tccatgtaca
     3301 gcttcatgtg catgttctcc ttaatcagct cgctcatggt accgggcccc ccctcgaggt
     3361 cgacggtatc gataagcttg atatcgaatt cctgcagccc gggggatcca ctagttctag
     3421 agcggccgcc accgcggtgg agctccaatt gtcctctcca aatgaaatga acttccttat
     3481 atagaggaag ggtcttgcga aggatagtgg gattgtgcgt catcccttac gtcagtggag
     3541 atgtcacatc aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg
     3601 ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg cagagagatc ttgaatgata
     3661 gcctttcctt tatcgcaatg atggcatttg taggagccac cttccttttc tactgtcctt
     3721 tcgatgaagt gacagatagc tgggcaatgg aatccgagga ggtttcccga aattatcctt
     3781 tgttgaaaag tctcaatagc cctttggtct tctgagactg tatctttgac atttttggag
     3841 tagaccagag tgtcgtgctc caccatgtta ccggtcgtac gaggcctatt aatcccgatc
     3901 tagtaacata gatgacaccg cgcgcgataa tttatcctag tttgcgcgct atattttgtt
     3961 ttctatcgcg tattaaatgt ataattgcgg gactctaatc ataaaaaccc atctcataaa
     4021 taacgtcatg cattacatgt taattattac atgcttaacg taattcaaca gaaattatat
     4081 gataatcatc gcaagaccgg caacaggatt caatcttaag aaactttatt gccaaatgtt
     4141 tgaacgatct cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg
     4201 agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc
     4261 aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca
     4321 gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc
     4381 atgggtcacg acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc
     4441 ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc
     4501 catccgagta cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc
     4561 cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg
     4621 agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct
     4681 tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca
     4741 cgatagccgc gctgcctcgt cctgaagttc attcagggca ccggacaggt cggtcttgac
     4801 aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat
     4861 tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc
     4921 gtgcaatcca tcttgttcaa tcatagatcc ggtgcagatt atttggattg agagtgaata
     4981 tgagactcta attggatacc gaggggaatt tatggaacgt cagtggagca tttttgacaa
     5041 gaaatatttg ctagctgata gtgaccttag gcgacttttg aacgcgcaat aatggtttct
     5101 gacgtatgtg cttagctcat taaactccag aaacccgcgg ctgagtggct ccttcaacgt
     5161 tgcggttctg tcagttccaa acgtaaaacg gcttgtcccg cgtcatcggc gggggtcata
     5221 acgtgactcc cttaattctc cgctcatgat cggcgcgcca cgtgacgcgt gtttacacca
     5281 caatatatcc tgccacgatt tgcccatggg ggtgggcgaa gaactccagc atgagatccc
     5341 cgcgctggag gatcatccag ccggcgtccc ggaaaacgat tccgaagccc aacctttcat
     5401 agaaggcggc ggtggaatcg aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca
     5461 tttcgaaccc cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg
     5521 ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc
     5581 aagctcttca gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc
     5641 cagccggcca cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa
     5701 gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg ggcatgcgcg ccttgagcct
     5761 ggcgaacagt tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac
     5821 aagaccggct tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa
     5881 tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac
     5941 tttctcggca ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag
     6001 cagccagtcc cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt
     6061 cgtggccagc cacgatagcc gcgctgcctc gtcctgaagt tcattcaggg caccggacag
     6121 gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc
     6181 agagcagccg attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc
     6241 cggagaacct gcgtgcaatc catcttgttc aatcatgcga aacgatcctc atcctgtctc
     6301 ttgatcagat cttgatcccc tgcgccatca gatccttggc ggcaagaaag ccatccagtt
     6361 tactttgcag ggcttcccaa ccttaccaga gggcgcccca gctggcaatt ccggttcgct
     6421 tgctgtccat aaaaccgccc agtctagcta tcgccatgta agcccactgc aagctacctg
     6481 ctttctcttt gcgcttgcgt tttcccttgt ccagatagcc cagtagctga cattcatccc
     6541 ag
//
