LOCUS       40924_47613        6557 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pYBA-1135, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6557)
  AUTHORS   Yao L, Yan X, Wang H, Cao M, Robaglia C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-NOV-2013) Beijing Agro-Biotechnology Research Center, 
            Beijing Academy of Agriculture and Forestry Sciences, 9 Shuguang 
            Huayuan Zhonglu, Beijing, Beijing 100097, P.R. China
REFERENCE   2  (bases 1 to 6557)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6557)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (19-NOV-2013) Beijing Agro-Biotechnology Research Center, Beijing 
            Academy of Agriculture and Forestry Sciences, 9 Shuguang Huayuan 
            Zhonglu, Beijing, Beijing 100097, P.R. China"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6557
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      741..1510
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             1511..2170
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     misc_feature    2368..2391
                     /label=RB 'overdrive' sequence
                     /note="RB 'overdrive' sequence"
     misc_feature    complement(2392..2416)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    2428..2628
                     /note="CaMV poly-A signal; derived from pRT103"
     CDS             complement(2645..3352)
                     /label=Venus
                     /note="yellow fluorescent protein (YFP) with fast and
                     efficient maturation (Nagai et al., 2002)"
     misc_feature    complement(3353..3460)
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(3465..3810)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      complement(3912..4164)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4168..4959)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(4968..5151)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(5286..5310)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(5500..6291)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag ctagcttgag
      601 tatactcact agactttgct tcgcaaagtc gtgaccgcct acggcggctg cggcgcccta
      661 cgggcttgct ctccgggctt cgccctgcgc ggtcgctgcg ctcccttgcc agcccgtgga
      721 tatgtggacg atggccgcga gcggccaccg gctggctcgc ttcgctcggc ccgtggacaa
      781 ccctgctgga caagctgatg gacaggctgc gcctgcccac gagcttgacc acagggattg
      841 cccaccggct acccagcctt cgaccacata cccaccggct ccaactgcgc ggcctgcggc
      901 cttgccccat caattttttt aattttctct ggggaaaagc ctccggcctg cggcctgcgc
      961 gcttcgcttg ccggttggac accaagtgga aggcgggtca aggctcgcgc agcgaccgcg
     1021 cagcggcttg gccttgacgc gcctggaacg acccaagcct atgcgagtgg gggcagtcga
     1081 aggcgaagcc cgcccgcctg ccccccgagc ctcacggcgg cgagtgcggg ggttccaagg
     1141 gggcagcgcc accttgggca aggccgaagg ccgcgcagtc gatcaacaag ccccggaggg
     1201 gccacttttt gccggagggg gagccgcgcc gaaggcgtgg gggaaccccg caggggtgcc
     1261 cttctttggg caccaaagaa ctagatatag ggcgaaatgc gaaagactta aaaatcaaca
     1321 acttaaaaaa ggggggtacg caacagctca ttgcggcacc ccccgcaata gctcattgcg
     1381 taggttaaag aaaatctgta attgactgcc acttttacgc aacgcataat tgttgtcgcg
     1441 ctgccgaaaa gttgcagctg attgcgcatg gtgccgcaac cgtgcggcac cctaccgcat
     1501 ggagataagc atggccacgc agtccagaga aatcggcatt caagccaaga acaagcccgg
     1561 tcactgggtg caaacggaac gcaaagcgca tgaggcgtgg gccgggctta ttgcgaggaa
     1621 acccacggcg gcaatgctgc tgcatcacct cgtggcgcag atgggccacc agaacgccgt
     1681 ggtggtcagc cagaagacac tttccaagct catcggacgt tctttgcgga cggtccaata
     1741 cgcagtcaag gacttggtgg ccgagcgctg gatctccgtc gtgaagctca acggccccgg
     1801 caccgtgtcg gcctacgtgg tcaatgaccg cgtggcgtgg ggccagcccc gcgaccagtt
     1861 gcgcctgtcg gtgttcagtg ccgccgtggt ggttgatcac gacgaccagg acgaatcgct
     1921 gttggggcat ggcgacctgc gccgcatccc gaccctgtat ccgggcgagc agcaactacc
     1981 gaccggcccc ggcgaggagc cgcccagcca gcccggcatt ccgggcatgg aaccagacct
     2041 gccagccttg accgaaacgg aggaatggga acggcgcggg cagcagcgcc tgccgatgcc
     2101 cgatgagccg tgttttctgg acgatggcga gccgttggag ccgccgacac gggtcacgct
     2161 gccgcgccgg tagcacttgg gttgcgcagc aacccgtaag tgcgctgttc cagactatcg
     2221 gctgtagccg cctcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat
     2281 ggagccgggc cacctcgacc tgaatggaag ccggcggcac ctcgctaacg gattcaccgt
     2341 ttttatcagg ctctgggagg cagaatacaa acaaacacat acagcgactt agtttacccg
     2401 ccaatatatc ctgtcaacgc gtttaaactg gattttggtt ttaggaatta gaaattttat
     2461 tgatagaagt attttacaaa tacaaataca tactaagggt ttcttatatg ctcaacacat
     2521 gagcgaaacc ctataagaac cctaattccc ttatctggga actactcaca cattattctg
     2581 gagaaaaata gagagagata gatttgtaga gagagactgg tgatttgcta gttagtcatc
     2641 gcgactcgtc catgccgaga gtgatcccgg cggcggtcac gaactccagc aggaccatgt
     2701 gatcgcgctt ctcgttgggg tctttgctca gggcggactg gtagctcagg tagtggttgt
     2761 cgggcagcag cacggggccg tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct
     2821 gcacgccgcc gtcctcgatg ttgtggcgga tcttgaagtt ggccttgatg ccgttcttct
     2881 gcttgtcggc ggtgatatag acgttgtggc tgttgtagtt gtactccagc ttgtgcccca
     2941 ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc gatgcggttc accagggtgt
     3001 cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg
     3061 tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt
     3121 ggtcggggta gcgggcgaag cactgcaggc cgtagcccag ggtggtcacg agggtgggcc
     3181 agggcacggg cagcttgccg gtggtgcaga tcagcttcag ggtcagcttg ccgtaggtgg
     3241 catcgccctc gccctcgccg gacacgctga acttgtggcc gtttacgtcg ccgtccagct
     3301 cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc cttgctcacc atggtaccgg
     3361 gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattcctgc agcccggggg
     3421 atccactagt tctagagcgg ccgccaccgc ggtggagctc caattgtcct ctccaaatga
     3481 aatgaacttc cttatataga ggaagggtct tgcgaaggat agtgggattg tgcgtcatcc
     3541 cttacgtcag tggagatgtc acatcaatcc acttgctttg aagacgtggt tggaacgtct
     3601 tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact gtcggcagag
     3661 agatcttgaa tgatagcctt tcctttatcg caatgatggc atttgtagga gccaccttcc
     3721 ttttctactg tcctttcgat gaagtgacag atagctgggc aatggaatcc gaggaggttt
     3781 cccgaaatta tcctttgttg aaaagtctca atagcccttt ggtcttctga gactgtatct
     3841 ttgacatttt tggagtagac cagagtgtcg tgctccacca tgttaccggt cgtacgaggc
     3901 ctattaatcc cgatctagta acatagatga caccgcgcgc gataatttat cctagtttgc
     3961 gcgctatatt ttgttttcta tcgcgtatta aatgtataat tgcgggactc taatcataaa
     4021 aacccatctc ataaataacg tcatgcatta catgttaatt attacatgct taacgtaatt
     4081 caacagaaat tatatgataa tcatcgcaag accggcaaca ggattcaatc ttaagaaact
     4141 ttattgccaa atgtttgaac gatctcagaa gaactcgtca agaaggcgat agaaggcgat
     4201 gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc
     4261 gccaagctct tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac
     4321 acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg
     4381 caagcaggca tcgccatggg tcacgacgag atcctcgccg tcgggcatgc gcgccttgag
     4441 cctggcgaac agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc
     4501 gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc
     4561 gaatgggcag gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga
     4621 tactttctcg gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa
     4681 tagcagccag tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc
     4741 cgtcgtggcc agccacgata gccgcgctgc ctcgtcctga agttcattca gggcaccgga
     4801 caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc
     4861 atcagagcag ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc
     4921 ggccggagaa cctgcgtgca atccatcttg ttcaatcata gatccggtgc agattatttg
     4981 gattgagagt gaatatgaga ctctaattgg ataccgaggg gaatttatgg aacgtcagtg
     5041 gagcattttt gacaagaaat atttgctagc tgatagtgac cttaggcgac ttttgaacgc
     5101 gcaataatgg tttctgacgt atgtgcttag ctcattaaac tccagaaacc cgcggctgag
     5161 tggctccttc aacgttgcgg ttctgtcagt tccaaacgta aaacggcttg tcccgcgtca
     5221 tcggcggggg tcataacgtg actcccttaa ttctccgctc atgatcggcg cgccacgtga
     5281 cgcgtgttta caccacaata tatcctgcca cgatttgccc atgggggtgg gcgaagaact
     5341 ccagcatgag atccccgcgc tggaggatca tccagccggc gtcccggaaa acgattccga
     5401 agcccaacct ttcatagaag gcggcggtgg aatcgaaatc tcgtgatggc aggttgggcg
     5461 tcgcttggtc ggtcatttcg aaccccagag tcccgctcag aagaactcgt caagaaggcg
     5521 atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc
     5581 agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata
     5641 gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac
     5701 catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat
     5761 gcgcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag
     5821 atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt
     5881 cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc
     5941 agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg
     6001 cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc
     6061 gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct gaagttcatt
     6121 cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg
     6181 gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct
     6241 ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga
     6301 tcctcatcct gtctcttgat cagatcttga tcccctgcgc catcagatcc ttggcggcaa
     6361 gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg ccccagctgg
     6421 caattccggt tcgcttgctg tccataaaac cgcccagtct agctatcgcc atgtaagccc
     6481 actgcaagct acctgctttc tctttgcgct tgcgttttcc cttgtccaga tagcccagta
     6541 gctgacattc atcccag
//
