LOCUS       40924_47548        7242 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pYBA-1121, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7242)
  AUTHORS   Yao L, Yan X, Wang H, Cao M, Robaglia C.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-NOV-2013) Beijing Agro-Biotechnology Research Center, 
            Beijing Academy of Agriculture and Forestry Sciences, 9 Shuguang 
            Huayuan Zhonglu, Beijing, Beijing 100097, P.R. China
REFERENCE   2  (bases 1 to 7242)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7242)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (20-NOV-2013) Beijing Agro-Biotechnology Research Center, Beijing 
            Academy of Agriculture and Forestry Sciences, 9 Shuguang Huayuan 
            Zhonglu, Beijing, Beijing 100097, P.R. China"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7242
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      741..1510
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             1511..2170
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     misc_feature    2368..2391
                     /label=RB 'overdrive' sequence
                     /note="RB 'overdrive' sequence"
     misc_feature    complement(2392..2416)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    2428..2628
                     /note="CaMV poly-A signal; derived from pRT103"
     CDS             complement(2638..4446)
                     /label=GUS
                     /note="beta-glucuronidase"
     misc_feature    complement(4447..4554)
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     terminator      complement(4597..4849)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4853..5644)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(5653..5836)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(5971..5995)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(6185..6976)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag ctagcttgag
      601 tatactcact agactttgct tcgcaaagtc gtgaccgcct acggcggctg cggcgcccta
      661 cgggcttgct ctccgggctt cgccctgcgc ggtcgctgcg ctcccttgcc agcccgtgga
      721 tatgtggacg atggccgcga gcggccaccg gctggctcgc ttcgctcggc ccgtggacaa
      781 ccctgctgga caagctgatg gacaggctgc gcctgcccac gagcttgacc acagggattg
      841 cccaccggct acccagcctt cgaccacata cccaccggct ccaactgcgc ggcctgcggc
      901 cttgccccat caattttttt aattttctct ggggaaaagc ctccggcctg cggcctgcgc
      961 gcttcgcttg ccggttggac accaagtgga aggcgggtca aggctcgcgc agcgaccgcg
     1021 cagcggcttg gccttgacgc gcctggaacg acccaagcct atgcgagtgg gggcagtcga
     1081 aggcgaagcc cgcccgcctg ccccccgagc ctcacggcgg cgagtgcggg ggttccaagg
     1141 gggcagcgcc accttgggca aggccgaagg ccgcgcagtc gatcaacaag ccccggaggg
     1201 gccacttttt gccggagggg gagccgcgcc gaaggcgtgg gggaaccccg caggggtgcc
     1261 cttctttggg caccaaagaa ctagatatag ggcgaaatgc gaaagactta aaaatcaaca
     1321 acttaaaaaa ggggggtacg caacagctca ttgcggcacc ccccgcaata gctcattgcg
     1381 taggttaaag aaaatctgta attgactgcc acttttacgc aacgcataat tgttgtcgcg
     1441 ctgccgaaaa gttgcagctg attgcgcatg gtgccgcaac cgtgcggcac cctaccgcat
     1501 ggagataagc atggccacgc agtccagaga aatcggcatt caagccaaga acaagcccgg
     1561 tcactgggtg caaacggaac gcaaagcgca tgaggcgtgg gccgggctta ttgcgaggaa
     1621 acccacggcg gcaatgctgc tgcatcacct cgtggcgcag atgggccacc agaacgccgt
     1681 ggtggtcagc cagaagacac tttccaagct catcggacgt tctttgcgga cggtccaata
     1741 cgcagtcaag gacttggtgg ccgagcgctg gatctccgtc gtgaagctca acggccccgg
     1801 caccgtgtcg gcctacgtgg tcaatgaccg cgtggcgtgg ggccagcccc gcgaccagtt
     1861 gcgcctgtcg gtgttcagtg ccgccgtggt ggttgatcac gacgaccagg acgaatcgct
     1921 gttggggcat ggcgacctgc gccgcatccc gaccctgtat ccgggcgagc agcaactacc
     1981 gaccggcccc ggcgaggagc cgcccagcca gcccggcatt ccgggcatgg aaccagacct
     2041 gccagccttg accgaaacgg aggaatggga acggcgcggg cagcagcgcc tgccgatgcc
     2101 cgatgagccg tgttttctgg acgatggcga gccgttggag ccgccgacac gggtcacgct
     2161 gccgcgccgg tagcacttgg gttgcgcagc aacccgtaag tgcgctgttc cagactatcg
     2221 gctgtagccg cctcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat
     2281 ggagccgggc cacctcgacc tgaatggaag ccggcggcac ctcgctaacg gattcaccgt
     2341 ttttatcagg ctctgggagg cagaatacaa acaaacacat acagcgactt agtttacccg
     2401 ccaatatatc ctgtcaacgc gtttaaactg gattttggtt ttaggaatta gaaattttat
     2461 tgatagaagt attttacaaa tacaaataca tactaagggt ttcttatatg ctcaacacat
     2521 gagcgaaacc ctataagaac cctaattccc ttatctggga actactcaca cattattctg
     2581 gagaaaaata gagagagata gatttgtaga gagagactgg tgatttgctc gcgatcattg
     2641 tttgcctccc tgctgcggtt tttcaccgaa gttcatgcca gtccagcgtt tttgcagcag
     2701 aaaagccgcc gacttcggtt tgcggtcgcg agtgaagatc cctttcttgt taccgccaac
     2761 gcgcaatatg ccttgcgagg tcgcaaaatc ggcgaaattc catacctgtt caccgacgac
     2821 ggcgctgacg cgatcaaaga cgcggtgata catatccagc catgcacact gatactcttc
     2881 actccacatg tcggtgtaca ttgagtgcag cccggctaac gtatccacgc cgtattcggt
     2941 gatgataatc ggctgatgca gtttctcctg ccaggccaga agttcttttt ccagtacctt
     3001 ctctgccgtt tccaaatcgc cgctttggac ataccatccg taataacggt tcaggcacag
     3061 cacatcaaag agatcgctga tggtatcggt gtgagcgtcg cagaacatta cattgacgca
     3121 ggtgatcgga cgcgtcgggt cgagtttacg cgttgcttcc gccagtggcg cgaaatattc
     3181 ccgtgcacct tgcggacggg tatccggttc gttggcaata ctccacatca ccacgcttgg
     3241 gtggtttttg tcacgcgcta tcagctcttt aatcgcctgt aagtgcgctt gctgagtttc
     3301 cccgttgact gcctcttcgc tgtacagttc tttcggcttg ttgcccgctt cgaaaccaat
     3361 gcctaaagag aggttaaagc cgacagcagc agtttcatca atcaccacga tgccatgttc
     3421 atctgcccag tcgagcatct cttcagcgta agggtaatgc gaggtacggt aggagttggc
     3481 cccaatccag tccattaatg cgtggtcgtg caccatcagc acgttatcga atcctttgcc
     3541 acgcaagtcc gcatcttcat gacgaccaaa gccagtaaag tagaacggtt tgtggttaat
     3601 caggaactgt tcgcccttca ctgccactga ccggatgccg acgcgaagcg ggtagatatc
     3661 acactctgtc tggcttttgg ctgtgacgca cagttcatag agataacctt cacccggttg
     3721 ccagaggtgc ggattcacca cttgcaaagt cccgctagtg ccttgtccag ttgcaaccac
     3781 ctgttgatcc gcatcacgca gttcaacgct gacatcacca ttggccacca cctgccagtc
     3841 aacagacgcg tggttacagt cttgcgcgac atgcgtcacc acggtgatat cgtccaccca
     3901 ggtgttcggc gtggtgtaga gcattacgct gcgatggatt ccggcatagt taaagaaatc
     3961 atggaagtaa gactgctttt tcttgccgtt ttcgtcggta atcaccattc ccggcgggat
     4021 agtctgccag ttcagttcgt tgttcacaca aacggtgata cgtacacttt tcccggcaat
     4081 aacatacggc gtgacatcgg cttcaaatgg cgtatagccg ccctgatgct ccatcacttc
     4141 ctgattattg acccacactt tgccgtaatg agtgaccgca tcgaaacgca gcacgatacg
     4201 ctggcctgcc caacctttcg gtataaagac ttcgcgctga taccagacgt tgcccgcata
     4261 attacgaata tctgcatcgg cgaactgatc gttaaaactg cctggcacag caattgcccg
     4321 gctttcttgt aacgcgcttt cccaccaacg ctgatcaatt ccacagtttt cgcgatccag
     4381 actgaatgcc cacaggccgt cgagtttttt gatttcacgg gttggggttt ctacaggacg
     4441 taacatggta ccgggccccc cctcgaggtc gacggtatcg ataagcttga tatcgaattc
     4501 ctgcagcccg ggggatccac tagttctaga gcggccgcca ccgcggtgga gctccaattg
     4561 gccctataga ccggtcgtac gaggcctatt aatcccgatc tagtaacata gatgacaccg
     4621 cgcgcgataa tttatcctag tttgcgcgct atattttgtt ttctatcgcg tattaaatgt
     4681 ataattgcgg gactctaatc ataaaaaccc atctcataaa taacgtcatg cattacatgt
     4741 taattattac atgcttaacg taattcaaca gaaattatat gataatcatc gcaagaccgg
     4801 caacaggatt caatcttaag aaactttatt gccaaatgtt tgaacgatct cagaagaact
     4861 cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca
     4921 cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg gtagccaacg
     4981 ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat ccagaaaagc
     5041 ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg acgagatcct
     5101 cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg agcccctgat
     5161 gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct
     5221 cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc
     5281 gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga gatgacagga
     5341 gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca gtgacaacgt
     5401 cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc gctgcctcgt
     5461 cctgaagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct
     5521 gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt gcccagtcat
     5581 agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca tcttgttcaa
     5641 tcatagatcc ggtgcagatt atttggattg agagtgaata tgagactcta attggatacc
     5701 gaggggaatt tatggaacgt cagtggagca tttttgacaa gaaatatttg ctagctgata
     5761 gtgaccttag gcgacttttg aacgcgcaat aatggtttct gacgtatgtg cttagctcat
     5821 taaactccag aaacccgcgg ctgagtggct ccttcaacgt tgcggttctg tcagttccaa
     5881 acgtaaaacg gcttgtcccg cgtcatcggc gggggtcata acgtgactcc cttaattctc
     5941 cgctcatgat cggcgcgcca cgtgacgcgt gtttacacca caatatatcc tgccacgatt
     6001 tgcccatggg ggtgggcgaa gaactccagc atgagatccc cgcgctggag gatcatccag
     6061 ccggcgtccc ggaaaacgat tccgaagccc aacctttcat agaaggcggc ggtggaatcg
     6121 aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca tttcgaaccc cagagtcccg
     6181 ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga
     6241 taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca gcaatatcac
     6301 gggtagccaa cgctatgtcc tgatagcggt ccgccacacc cagccggcca cagtcgatga
     6361 atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg ccatgggtca
     6421 cgacgagatc ctcgccgtcg ggcatgcgcg ccttgagcct ggcgaacagt tcggctggcg
     6481 cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct tccatccgag
     6541 tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa
     6601 gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca ggagcaaggt
     6661 gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc cttcccgctt
     6721 cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc
     6781 gcgctgcctc gtcctgaagt tcattcaggg caccggacag gtcggtcttg acaaaaagaa
     6841 ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg attgtctgtt
     6901 gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct gcgtgcaatc
     6961 catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcagat cttgatcccc
     7021 tgcgccatca gatccttggc ggcaagaaag ccatccagtt tactttgcag ggcttcccaa
     7081 ccttaccaga gggcgcccca gctggcaatt ccggttcgct tgctgtccat aaaaccgccc
     7141 agtctagcta tcgccatgta agcccactgc aagctacctg ctttctcttt gcgcttgcgt
     7201 tttcccttgt ccagatagcc cagtagctga cattcatccc ag
//
