LOCUS       40924_47533        5440 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pYBA-1103, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5440)
  AUTHORS   Yao L, Yan X, Wang H, Cao M, Robaglia C.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-DEC-2015) Beijing Agro-Biotechnology Research Center, 
            Beijing Academy of Agriculture and Forestry Sciences, 9 Shuguang 
            Huayuan Zhonglu, Beijing 100097, P.R. China
REFERENCE   2  (bases 1 to 5440)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5440)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (02-DEC-2015) Beijing Agro-Biotechnology Research Center, Beijing 
            Academy of Agriculture and Forestry Sciences, 9 Shuguang Huayuan 
            Zhonglu, Beijing 100097, P.R. China"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5440
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      741..1510
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             1511..2170
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     misc_feature    2368..2391
                     /label=RB 'overdrive' sequence
                     /note="RB 'overdrive' sequence"
     misc_feature    complement(2392..2416)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     regulatory      2428..2628
                     /note="CaMV poly-A signal; derived from pRT103"
                     /regulatory_class="polyA_signal_sequence"
     misc_feature    complement(2645..2752)
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     terminator      complement(2795..3047)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3051..3842)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(3851..4034)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(4169..4193)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(4383..5174)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag ctagcttgag
      601 tatactcact agactttgct tcgcaaagtc gtgaccgcct acggcggctg cggcgcccta
      661 cgggcttgct ctccgggctt cgccctgcgc ggtcgctgcg ctcccttgcc agcccgtgga
      721 tatgtggacg atggccgcga gcggccaccg gctggctcgc ttcgctcggc ccgtggacaa
      781 ccctgctgga caagctgatg gacaggctgc gcctgcccac gagcttgacc acagggattg
      841 cccaccggct acccagcctt cgaccacata cccaccggct ccaactgcgc ggcctgcggc
      901 cttgccccat caattttttt aattttctct ggggaaaagc ctccggcctg cggcctgcgc
      961 gcttcgcttg ccggttggac accaagtgga aggcgggtca aggctcgcgc agcgaccgcg
     1021 cagcggcttg gccttgacgc gcctggaacg acccaagcct atgcgagtgg gggcagtcga
     1081 aggcgaagcc cgcccgcctg ccccccgagc ctcacggcgg cgagtgcggg ggttccaagg
     1141 gggcagcgcc accttgggca aggccgaagg ccgcgcagtc gatcaacaag ccccggaggg
     1201 gccacttttt gccggagggg gagccgcgcc gaaggcgtgg gggaaccccg caggggtgcc
     1261 cttctttggg caccaaagaa ctagatatag ggcgaaatgc gaaagactta aaaatcaaca
     1321 acttaaaaaa ggggggtacg caacagctca ttgcggcacc ccccgcaata gctcattgcg
     1381 taggttaaag aaaatctgta attgactgcc acttttacgc aacgcataat tgttgtcgcg
     1441 ctgccgaaaa gttgcagctg attgcgcatg gtgccgcaac cgtgcggcac cctaccgcat
     1501 ggagataagc atggccacgc agtccagaga aatcggcatt caagccaaga acaagcccgg
     1561 tcactgggtg caaacggaac gcaaagcgca tgaggcgtgg gccgggctta ttgcgaggaa
     1621 acccacggcg gcaatgctgc tgcatcacct cgtggcgcag atgggccacc agaacgccgt
     1681 ggtggtcagc cagaagacac tttccaagct catcggacgt tctttgcgga cggtccaata
     1741 cgcagtcaag gacttggtgg ccgagcgctg gatctccgtc gtgaagctca acggccccgg
     1801 caccgtgtcg gcctacgtgg tcaatgaccg cgtggcgtgg ggccagcccc gcgaccagtt
     1861 gcgcctgtcg gtgttcagtg ccgccgtggt ggttgatcac gacgaccagg acgaatcgct
     1921 gttggggcat ggcgacctgc gccgcatccc gaccctgtat ccgggcgagc agcaactacc
     1981 gaccggcccc ggcgaggagc cgcccagcca gcccggcatt ccgggcatgg aaccagacct
     2041 gccagccttg accgaaacgg aggaatggga acggcgcggg cagcagcgcc tgccgatgcc
     2101 cgatgagccg tgttttctgg acgatggcga gccgttggag ccgccgacac gggtcacgct
     2161 gccgcgccgg tagcacttgg gttgcgcagc aacccgtaag tgcgctgttc cagactatcg
     2221 gctgtagccg cctcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat
     2281 ggagccgggc cacctcgacc tgaatggaag ccggcggcac ctcgctaacg gattcaccgt
     2341 ttttatcagg ctctgggagg cagaatacaa acaaacacat acagcgactt agtttacccg
     2401 ccaatatatc ctgtcaacgc gtttaaactg gattttggtt ttaggaatta gaaattttat
     2461 tgatagaagt attttacaaa tacaaataca tactaagggt ttcttatatg ctcaacacat
     2521 gagcgaaacc ctataagaac cctaattccc ttatctggga actactcaca cattattctg
     2581 gagaaaaata gagagagata gatttgtaga gagagactgg tgatttgcta gttagtcatc
     2641 gcgaggtacc gggccccccc tcgaggtcga cggtatcgat aagcttgata tcgaattcct
     2701 gcagcccggg ggatccacta gttctagagc ggccgccacc gcggtggagc tccaattggc
     2761 cctatagacc ggtcgtacga ggcctattaa tcccgatcta gtaacataga tgacaccgcg
     2821 cgcgataatt tatcctagtt tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat
     2881 aattgcggga ctctaatcat aaaaacccat ctcataaata acgtcatgca ttacatgtta
     2941 attattacat gcttaacgta attcaacaga aattatatga taatcatcgc aagaccggca
     3001 acaggattca atcttaagaa actttattgc caaatgtttg aacgatctca gaagaactcg
     3061 tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg
     3121 aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct
     3181 atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg
     3241 ccattttcca ccatgatatt cggcaagcag gcatcgccat gggtcacgac gagatcctcg
     3301 ccgtcgggca tgcgcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc
     3361 tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg
     3421 atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc
     3481 cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga
     3541 tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg
     3601 agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtcc
     3661 tgaagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc
     3721 gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag
     3781 ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc
     3841 atagatccgg tgcagattat ttggattgag agtgaatatg agactctaat tggataccga
     3901 ggggaattta tggaacgtca gtggagcatt tttgacaaga aatatttgct agctgatagt
     3961 gaccttaggc gacttttgaa cgcgcaataa tggtttctga cgtatgtgct tagctcatta
     4021 aactccagaa acccgcggct gagtggctcc ttcaacgttg cggttctgtc agttccaaac
     4081 gtaaaacggc ttgtcccgcg tcatcggcgg gggtcataac gtgactccct taattctccg
     4141 ctcatgatcg gcgcgccacg tgacgcgtgt ttacaccaca atatatcctg ccacgatttg
     4201 cccatggggg tgggcgaaga actccagcat gagatccccg cgctggagga tcatccagcc
     4261 ggcgtcccgg aaaacgattc cgaagcccaa cctttcatag aaggcggcgg tggaatcgaa
     4321 atctcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct
     4381 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
     4441 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
     4501 gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat
     4561 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
     4621 acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg
     4681 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
     4741 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
     4801 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
     4861 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
     4921 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
     4981 gctgcctcgt cctgaagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
     5041 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
     5101 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
     5161 tcttgttcaa tcatgcgaaa cgatcctcat cctgtctctt gatcagatct tgatcccctg
     5221 cgccatcaga tccttggcgg caagaaagcc atccagttta ctttgcaggg cttcccaacc
     5281 ttaccagagg gcgccccagc tggcaattcc ggttcgcttg ctgtccataa aaccgcccag
     5341 tctagctatc gccatgtaag cccactgcaa gctacctgct ttctctttgc gcttgcgttt
     5401 tcccttgtcc agatagccca gtagctgaca ttcatcccag
//
