LOCUS       40924_47508       13280 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pYB2301, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13280)
  AUTHORS   Shih PM, Vuu K, Mansoori N, Ayad L, Louie KB, Bowen BP, Northen TR, 
            Loque D.
  TITLE     A robust gene-stacking method utilizing yeast assembly for plant 
            synthetic biology
  JOURNAL   Nat Commun 7, 13215 (2016)
  PUBMED    27782150
REFERENCE   2  (bases 1 to 13280)
  AUTHORS   Shih PM, Vuu K, Mansoori N, Ayad L, Louie K, Bowen BP, Northen TR, 
            Loque D.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-SEP-2016) Feedstocks Division, Joint Bioenergy 
            Institute, 5885 Hollis St., Emeryville, CA 946082405, USA
REFERENCE   3  (bases 1 to 13280)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13280)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun 
            7, 13215 (2016)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-SEP-2016) Feedstocks Division, Joint Bioenergy Institute, 5885 
            Hollis St., Emeryville, CA 946082405, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13280
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(36..742)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        754..969
                     /label=URA3 promoter
     CDS             970..1770
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     terminator      1857..2107
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2157..2181
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3481..4107
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4544..5608
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      5677..5871
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6215..6355
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6541..7129)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        7385..7792
                     /label=LEU2 promoter
     CDS             7793..8884
                     /codon_start=1
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     misc_feature    complement(9370..9871)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     CDS             complement(9970..10761)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    11186..11210
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(11288..11462)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(11522..12310)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(12379..13056)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    13247..13268
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
ORIGIN
        1 acgcgtcctg caggcgcgcc gaggggatcg agcccctgct gagcctcgac atgttgtcgc
       61 aaaattcgcc ctggacccgc ccaacgattt gtcgtcactg tcaaggtttg acctgcactt
      121 catttggggc ccacatacac caaaaaaatg ctgcataatt ctcggggcag caagtcggtt
      181 acccggccgc cgtgctggac cgggttgaat ggtgcccgta actttcggta gagcggacgg
      241 ccaatactca acttcaagga atctcaccca tgcgcgccgg cggggaaccg gagttccctt
      301 cagtgaacgt tattagttcg ccgctcggtg tgtcgtagat actagcccct ggggcctttt
      361 gaaatttgaa taagatttat gtaatcagtc ttttaggttt gaccggttct gccgcttttt
      421 ttaaaattgg atttgtaata ataaaacgca attgtttgtt attgtggcgc tctatcatag
      481 atgtcgctat aaacctattc agcacaatat attgttttca ttttaatatt gtacatataa
      541 gtagtagggt acaatcagta aattgaacgg agaatattat tcataaaaat acgatagtaa
      601 cgggtgatat attcattaga atgaaccgaa accggcggta aggatctgag ctacacatgc
      661 tcaggttttt tacaacgtgc acaacagaat tgaaagcaaa tatcatgcga tcataggcgt
      721 ctcgcatatc tcattaaagc agcacgtgag cttttcaatt catcattttt tttttattct
      781 tttttttgat ttcggtttcc ttgaaatttt tttgattcgg taatctccga acagaaggaa
      841 gaacgaagga aggagcacag acttagattg gtatatatac gcatatgtag tgttgaagaa
      901 acatgaaatt gcccagtatt cttaacccaa ctgcacagaa caaaaacatg caggaaacga
      961 agataaatca tgtcgaaagc tacatataag gaacgtgctg ctactcatcc tagtcctgtt
     1021 gctgccaagc tatttaatat catgcacgaa aagcaaacaa acttgtgtgc ttcattggat
     1081 gttcgtacca ccaaggaatt actggagtta gttgaagcat taggtcccaa aatttgttta
     1141 ctaaaaacac atgtggatat cttgactgat ttttccatgg agggcacagt taagccgcta
     1201 aaggcattat ccgccaagta caatttttta ctcttcgaag acagaaaatt tgctgacatt
     1261 ggtaatacag tcaaattgca gtactctgcg ggtgtataca gaatagcaga atgggcagac
     1321 attacgaatg cacacggtgt ggtgggccca ggtattgtta gcggtttgaa gcaggcggca
     1381 gaagaagtaa caaaggaacc tagaggcctt ttgatgttag cagaattgtc atgcaagggc
     1441 tccctatcta ctggagaata tactaagggt actgttgaca ttgcgaagag cgacaaagat
     1501 tttgttatcg gctttattgc tcaaagagac atgggtggaa gagatgaagg ttacgattgg
     1561 ttgattatga cacccggtgt gggtttagat gacaagggag acgcattggg tcaacagtat
     1621 agaaccgtgg atgatgtggt ctctacagga tctgacatta ttattgttgg aagaggacta
     1681 tttgcaaagg gaagggatgc taaggtagag ggtgaacgtt acagaaaagc aggctgggaa
     1741 gcatatttga gaagatgcgg ccagcaaaac taaaaaactg tattataagt aaatgcatgt
     1801 atactaaact cacaaattag agcttcaatt taattatatc agttattacc ccacgtgcgt
     1861 tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt
     1921 atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg
     1981 ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata
     2041 gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta
     2101 ctagatcggg cctgcaggac gcgtggcgcg ccagatccaa ttaaactatc agtgtttgac
     2161 aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa cggatattta
     2221 aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa ccacagggtt
     2281 cccctcggga tcaaagtact ttgatccaac ccctccgctg ctatagtgca gtcggcttct
     2341 gacgttcagt gcagccgtct tctgaaaacg acatgtcgca caagtcctaa gttacgcgac
     2401 aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt
     2461 agaatacttg cgactagaac cggagacatt acgccatgaa caagagcgcc gccgctggcc
     2521 tgctgggcta tgcccgcgtc agcaccgacg accaggactt gaccaaccaa cgggccgaac
     2581 tgcacgcggc cggctgcacc aagctgtttt ccgagaagat caccggcacc aggcgcgacc
     2641 gcccggagct ggccaggatg cttgaccacc tacgccctgg cgacgttgtg acagtgacca
     2701 ggctagaccg cctggcccgc agcacccgcg acctactgga cattgccgag cgcatccagg
     2761 aggccggcgc gggcctgcgt agcctggcag agccgtgggc cgacaccacc acgccggccg
     2821 gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg
     2881 accgcacccg gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt ggcccccgcc
     2941 ctaccctcac cccggcacag atcgcgcacg cccgcgagct gatcgaccag gaaggccgca
     3001 ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg
     3061 agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg
     3121 cattgaccga ggccgacgcc ctggcggccg ccgagaatga acgccaagag gaacaagcat
     3181 gaaaccgcac caggacggcc aggacgaacc gtttttcatt accgaagaga tcgaggcgga
     3241 gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac gtctcaaccg tgcggctgca
     3301 tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc tggccggcca gcttggccgc
     3361 tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg
     3421 tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa caaatacgca aggggaacgc
     3481 atgaaggtta tcgctgtact taaccagaaa ggcgggtcag gcaagacgac catcgcaacc
     3541 catctagccc gcgccctgca actcgccggg gccgatgttc tgttagtcga ttccgatccc
     3601 cagggcagtg cccgcgattg ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc
     3661 atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg
     3721 atcgacggag cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc agccgacttc
     3781 gtgctgattc cggtgcagcc aagcccttac gacatatggg ccaccgccga cctggtggag
     3841 ctggttaagc agcgcattga ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg
     3901 cgggcgatca aaggcacgcg catcggcggt gaggttgccg aggcgctggc cgggtacgag
     3961 ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct acccaggcac tgccgccgcc
     4021 ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg
     4081 gccgctgaaa ttaaatcaaa actcatttga gttaatgagg taaagagaaa atgagcaaaa
     4141 gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg
     4201 ccagcctggc agacacgcca gccatgaagc gggtcaactt tcagttgccg gcggaggatc
     4261 acaccaagct gaagatgtac gcggtacgcc aaggcaagac cattaccgag ctgctatctg
     4321 aatacatcgc gcagctacca gagtaaatga gcaaatgaat aaatgagtag atgaatttta
     4381 gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc
     4441 cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg gctgggttgt ctgccggccc
     4501 tgcaatggca ctggaacccc caagcccgag gaatcggcgt gacggtcgca aaccatccgg
     4561 cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga aggccgcgca
     4621 ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc
     4681 cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc cgtcgattag
     4741 gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct atgacgtggg
     4801 cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg
     4861 acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg tttccgcagg
     4921 gccggccggc atggccagtg tgtgggatta cgacctggta ctgatggcgg tttcccatct
     4981 aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc gcgtgttccg
     5041 tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa agcagaaaga
     5101 cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc agcgtacgaa
     5161 gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga ttagccgcta
     5221 caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc tagctgattg
     5281 gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc accccgatta
     5341 ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg
     5401 caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca gcgccggaga
     5461 gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta
     5521 cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct accgcaacct
     5581 gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag ggcaaattgc
     5641 cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt acattgggaa
     5701 cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc cgtacattgg
     5761 gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt tttccgccta
     5821 aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat aactgtctgg
     5881 ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg
     5941 ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg
     6001 gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc
     6061 acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     6121 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     6181 agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg
     6241 atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     6301 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc
     6361 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     6421 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     6481 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     6541 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     6601 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     6661 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     6721 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     6781 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     6841 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     6901 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     6961 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     7021 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     7081 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagaattgcg
     7141 accgtcgagg agaacttcta gtatatctac atacctaata ttattgcctt attaaaaatg
     7201 gaatcccaac aattacatca aaatccacat tctcttcaaa atcaattgtc ctgtacttcc
     7261 ttgttcatgt gtgttcaaaa acgttatatt tataggataa ttatactcta tttctcaaca
     7321 agtaattggt tgtttggccg agcggtctaa ggcgcctgat tcaagaaata tcttgaccgc
     7381 agttaactgt gggaatactc aggtatcgta agatgcaaga gttcgaatct cttagcaacc
     7441 attatttttt tcctcaacat aacgagaaca cacaggggcg ctatcgcaca gaatcaaatt
     7501 cgatgactgg aaattttttg ttaatttcag aggtcgcctg acgcatatac ctttttcaac
     7561 tgaaaaattg ggagaaaaag gaaaggtgag agcgccggaa ccggcttttc atatagaata
     7621 gagaagcgtt catgactaaa tgcttgcatc acaatacttg aagttgacaa tattatttaa
     7681 ggacctattg ttttttccaa taggtggtta gcaatcgtct tactttctaa cttttcttac
     7741 cttttacatt tcagcaatat atatatatat atttcaagga tataccattc taatgtctgc
     7801 ccctaagaag atcgtcgttt tgccaggtga ccacgttggt caagaaatca cagccgaagc
     7861 cattaaggtt cttaaagcta tttctgatgt tcgttccaat gtcaagttcg atttcgaaaa
     7921 tcatttaatt ggtggtgctg ctatcgatgc tacaggtgtt ccacttccag atgaggcgct
     7981 ggaagcctcc aagaaggctg atgccgtttt gttaggtgct gtgggtggtc ctaaatgggg
     8041 aaccggtagt gttagacctg aacaaggttt actaaaaatc cgtaaagaac ttcaattgta
     8101 cgccaactta agaccatgta actttgcatc cgactctctt ttagacttat ctccaatcaa
     8161 gccacaattt gctaaaggta ctgacttcgt tgttgtcaga gaattagtgg gaggtattta
     8221 ctttggtaag agaaaggaag acgatggtga tggtgtcgct tgggatagtg aacaatacac
     8281 cgttccagaa gtgcaaagaa tcacaagaat ggccgctttc atggccctac aacatgagcc
     8341 accattgcct atttggtcct tggataaagc taatgttttg gcctcttcaa gattatggag
     8401 aaaaactgtg gaggaaacca tcaagaacga atttcctaca ttgaaggttc aacatcaatt
     8461 gattgattct gccgccatga tcctagttaa gaacccaacc cacctaaatg gtattataat
     8521 caccagcaac atgtttggtg atatcatctc cgatgaagcc tccgttatcc caggttcctt
     8581 gggtttgttg ccatctgcgt ccttggcctc tttgccagac aagaacaccg catttggttt
     8641 gtacgaacca tgccacggtt ctgctccaga tttgccaaag aataaggtca accctatcgc
     8701 cactatcttg tctgctgcaa tgatgttgaa attgtcattg aacttgcctg aagaaggtaa
     8761 ggccattgaa gatgcagtta aaaaggtttt ggatgcaggt atcagaactg gtgatttagg
     8821 tggttccaac agtaccaccg aagtcggtga tgctgtcgcc gaagaagtta agaaaatcct
     8881 tgcttaaaaa gattctcttt ttttatgata tttgtacata aactttataa atgaaattca
     8941 taatagaaac gacacgaaat tacaaaatgg aatatgttca tagggtagac gaaactatat
     9001 acgcaatcta catacattta tcaagaagga gaaaaaggag gatgtaaagg aatacaggta
     9061 agcaaattga tactaatggc tcaacgtgat aaggaaaaag aattgcactt taacattaat
     9121 attgacaagg aggagggcac cacacaaaaa gttaggtgta acagaaaatc atgaaactat
     9181 gattcctaat ttatatattg gaggattttc tctaaaaaaa aaaaaataca acaaataaaa
     9241 aacactcaat gacctgacca tttgatggag tttaagtcaa taccttcttg aaccatttcc
     9301 cataatggtg aaagttccct caagaatttt actctgtcag aaacggcctt aacgacgtag
     9361 tcgatgacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa tgatggaata
     9421 atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat tttagaaagt
     9481 aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa ggtttaaaaa
     9541 atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc agattaaata
     9601 gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg tgtgtggtct
     9661 tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa gagcaagata
     9721 aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa aacaaaaact
     9781 attttttctt taatttcttt ttttactttc tatttttaat ttatatattt atattaaaaa
     9841 atttaaatta taattatttt tatagcacgt catctgcgag ctcgatcctt tgatcttttc
     9901 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgcattc
     9961 taggtactaa aacaattcat ccagtaaaat ataatatttt attttctccc aatcaggctt
    10021 gatccccagt aagtcaaaaa atagctcgac atactgttct tccccgatat cctccctgat
    10081 cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc ctgccgcttc tcccaagatc
    10141 aataaagcca cttactttgc catctttcac aaagatgttg ctgtctccca ggtcgccgtg
    10201 ggaaaagaca agttcctctt cgggcttttc cgtctttaaa aaatcataca gctcgcgcgg
    10261 atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc acatcggcca gatcgttatt
    10321 cagtaagtaa tccaattcgg ctaagcggct gtctaagcta ttcgtatagg gacaatccga
    10381 tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac agctcgataa tcttttcagg
    10441 gctttgttca tcttcatact cttccgagca aaggacgcca tcggcctcac tcatgagcag
    10501 attgctccag ccatcatgcc gttcaaagtg caggaccttt ggaacaggca gctttccttc
    10561 cagccatagc atcatgtcct tttcccgttc cacatcatag gtggtccctt tataccggct
    10621 gtccgtcatt tttaaatata ggttttcatt ttctcccacc agcttatata ccttagcagg
    10681 agacattcct tccgtatctt ttacgcagcg gtatttttcg atcagttttt tcaattccgg
    10741 tgatattctc attttagcca tttattattt ccttcctctt ttctacagta tttaaagata
    10801 ccccaagaag ctaattataa caagacgaac tccaattcac tgttccttgc attctaaaac
    10861 cttaaatacc agaaaacagc tttttcaaag ttgttttcaa agttggcgta taacatagta
    10921 tcgacggagc cgattttgaa accgcggtga tcacaggcag caacgctctg tcatcgttac
    10981 aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc ttagttgccg
    11041 ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc
    11101 tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc
    11161 ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac aaattgacgc
    11221 ttagacaact taataacaca ttgcggacgt ttttaatgta ctgaattaac gccgaattaa
    11281 ttcgggggat ctggatttta gtactggatt ttggttttag gaattagaaa ttttattgat
    11341 agaagtattt tacaaataca aatacatact aagggtttct tatatgctca acacatgagc
    11401 gaaaccctat aggaacccta attcccttat ctgggaacta ctcacacatt attatggaga
    11461 aactcgagct tgtcgatcga ctctagctag aggatcgatc cgaaccccag agtcccgctc
    11521 agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac
    11581 cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg
    11641 tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag tcgatgaatc
    11701 cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca tgtgtcacga
    11761 cgagatcctc gccgtcgggc atgcgcgcct tgagcctggc gaacagttcg gctggcgcga
    11821 gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac
    11881 gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg
    11941 tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag
    12001 atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag
    12061 tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg
    12121 ctgcctcgtc ctggagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg
    12181 ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt gtctgttgtg
    12241 cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat
    12301 cttgttcaat ccccatggtc gatcgacaga tctgcgaaag ctcgagagag atagatttgt
    12361 agagagagac tggtgatttc agcgtgtcct ctccaaatga aatgaacttc cttatataga
    12421 ggaaggtctt gcgaaggata gtgggattgt gcgtcatccc ttacgtcagt ggagatatca
    12481 catcaatcca cttgctttga agacgtggtt ggaacgtctt ctttttccac gatgctcctc
    12541 gtgggtgggg gtccatcttt gggaccactg tcggcagagg catcttgaac gatagccttt
    12601 cctttatcgc aatgatggca tttgtaggtg ccaccttcct tttctactgt ccttttgatg
    12661 aagtgacaga tagctgggca atggaatccg aggaggtttc ccgatattac cctttgttga
    12721 aaagtctcaa tagccctttg gtcttctgag actgtatctt tgatattctt ggagtagacg
    12781 agagtgtcgt gctccaccat gttatcacat caatccactt gctttgaaga cgtggttgga
    12841 acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg
    12901 gcagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt gtaggtgcca
    12961 ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg gaatccgagg
    13021 aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctttggtc ttctgagact
    13081 gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt ggcaagctgc
    13141 tctagccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
    13201 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
    13261 tcactcatta ggcaactaga
//
