LOCUS       V001997                 8694 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V001997
VERSION     V001997
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8694)
  AUTHORS   Khatib A, Arhab Y, Bentebibel A, Abousalham A, Noiriel A.
  TITLE     Reassessing the Potential Activities of Plant CGI-58 Protein
  JOURNAL   PLoS ONE 11 (1), E0145806 (2016)
   PUBMED   26745266
REFERENCE   2  (bases 1 to 8694)
  AUTHORS   Khatib A, Arhab Y, Bentebibel A, Abousalham A, Noiriel A.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-OCT-2015) Organization and Dynamics of Biological
            Membranes, Insitut de Chimie et de Biochimie Moleculaires et
            Supramoleculaires, 43 bd du 11 Novembre 1918, Villeurbanne 69622,
            France
REFERENCE   3  (bases 1 to 8694)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8694)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2016"; volume: "11"; issue: "1"; pages: "E0145806"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-OCT-2015) Organization and Dynamics of Biological Membranes,
            Insitut de Chimie et de Biochimie Moleculaires et Supramoleculaires,
            43 bd du 11 Novembre 1918, Villeurbanne 69622, France"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8694
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(220..322)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      complement(848..1393)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     promoter        1505..1533
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     promoter        1884..1961
                     /label="lacI promoter"
     CDS             1962..3041
                     /label="lacI"
                     /note="lac repressor"
     protein_bind    3057..3078
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3093..3123
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    3131..3147
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             3167..3340
                     /label="lacZ-alpha"
                     /note="LacZ-alpha fragment of beta-galactosidase"
     CDS             3647..6295
                     /note="T7 RNA polymerase from Escherichia phage T7.
                     Accession#: P00573"
                     /label="T7 RNA polymerase"
     CDS             complement(join(8257..8694,1..219))
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
ORIGIN
        1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
       61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
      121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
      181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
      241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
      301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
      361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
      421 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt
      481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
      541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact
      601 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
      661 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
      721 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
      781 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
      841 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
      901 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc
      961 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
     1021 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
     1081 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt
     1141 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt
     1201 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg
     1261 tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt
     1321 cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc
     1381 aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca
     1441 atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc
     1501 atgtttgaca gcttatcatc gataagcttt aatgcggtag tttatcacag ttaaattgct
     1561 aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg
     1621 tcaccctgga tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg
     1681 atatcgtcca ttccgacagc atcgccagtc actatggcgt gctgctagcg ctatatgcgt
     1741 tgatgcaatt tctatgcgca cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc
     1801 cagtcctgct cgcttcgcta cttggagcca ctatcgacta cgcgatcatg gcgaccacac
     1861 ccgtcctgtg gatccagatc ccggacacca tcgaatggcg caaaaccttt cgcggtatgg
     1921 catgatagcg cccggaagag agtcaattca gggtggtgaa tgtgaaacca gtaacgttat
     1981 acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg
     2041 ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt
     2101 acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg attggcgttg
     2161 ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg
     2221 ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct
     2281 gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc attaactatc
     2341 cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt ccggcgttat
     2401 ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat gaagacggta
     2461 cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg
     2521 gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa tatctcactc
     2581 gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg tccggttttc
     2641 aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg gttgccaacg
     2701 atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg
     2761 atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc ccgccgttaa
     2821 ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac
     2881 tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa
     2941 aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     3001 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     3061 gtaagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtata
     3121 atgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     3181 ggattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     3241 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     3301 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc
     3361 ggcaccagaa gcggtgccgg aaagctggct ggagtgcgat cttcctgagg ccgatactgt
     3421 cgtcgtcccc tcaaactggc agatgcacgg ttacgatgcg cccatctaca ccaacgtgac
     3481 ctatcccatt acggtcaatc cgccgtttgt tcccacggag aatccgacgg gttgttactc
     3541 gctcacattt aatgttgatg aaagctggct acaggaaggc cagacgcgaa ttatttttga
     3601 tggcgtcggg atctgatccg gatttactaa ctggaagagg cactaaatga acacgattaa
     3661 catcgctaag aacgacttct ctgacatcga actggctgct atcccgttca acactctggc
     3721 tgaccattac ggtgagcgtt tagctcgcga acagttggcc cttgagcatg agtcttacga
     3781 gatgggtgaa gcacgcttcc gcaagatgtt tgagcgtcaa cttaaagctg gtgaggttgc
     3841 ggataacgct gccgccaagc ctctcatcac taccctactc cctaagatga ttgcacgcat
     3901 caacgactgg tttgaggaag tgaaagctaa gcgcggcaag cgcccgacag ccttccagtt
     3961 cctgcaagaa atcaagccgg aagccgtagc gtacatcacc attaagacca ctctggcttg
     4021 cctaaccagt gctgacaata caaccgttca ggctgtagca agcgcaatcg gtcgggccat
     4081 tgaggacgag gctcgcttcg gtcgtatccg tgaccttgaa gctaagcact tcaagaaaaa
     4141 cgttgaggaa caactcaaca agcgcgtagg gcacgtctac aagaaagcat ttatgcaagt
     4201 tgtcgaggct gacatgctct ctaagggtct actcggtggc gaggcgtggt cttcgtggca
     4261 taaggaagac tctattcatg taggagtacg ctgcatcgag atgctcattg agtcaaccgg
     4321 aatggttagc ttacaccgcc aaaatgctgg cgtagtaggt caagactctg agactatcga
     4381 actcgcacct gaatacgctg aggctatcgc aacccgtgca ggtgcgctgg ctggcatctc
     4441 tccgatgttc caaccttgcg tagttcctcc taagccgtgg actggcatta ctggtggtgg
     4501 ctattgggct aacggtcgtc gtcctctggc gctggtgcgt actcacagta agaaagcact
     4561 gatgcgctac gaagacgttt acatgcctga ggtgtacaaa gcgattaaca ttgcgcaaaa
     4621 caccgcatgg aaaatcaaca agaaagtcct agcggtcgcc aacgtaatca ccaagtggaa
     4681 gcattgtccg gtcgaggaca tccctgcgat tgagcgtgaa gaactcccga tgaaaccgga
     4741 agacatcgac atgaatcctg aggctctcac cgcgtggaaa cgtgctgccg ctgctgtgta
     4801 ccgcaaggac agggctcgca agtctcgccg tatcagcctt gagttcatgc ttgagcaagc
     4861 caataagttt gctaaccata aggccatctg gttcccttac aacatggact ggcgcggtcg
     4921 tgtttacgcc gtgtcaatgt tcaacccgca aggtaacgat atgaccaaag gactgcttac
     4981 gctggcgaaa ggtaaaccaa tcggtaagga aggttactac tggctgaaaa tccacggtgc
     5041 aaactgtgcg ggtgtcgata aggttccgtt ccctgagcgc atcaagttca ttgaggaaaa
     5101 ccacgagaac atcatggctt gcgctaagtc tccactggag aacacttggt gggctgagca
     5161 agattctccg ttctgcttcc ttgcgttctg ctttgagtac gctggggtac agcaccacgg
     5221 cctgagctat aactgctccc ttccgctggc gtttgacggg tcttgctctg gcatccagca
     5281 cttctccgcg atgctccgag atgaggtagg tggtcgcgcg gttaacttgc ttcctagtga
     5341 gaccgttcag gacatctacg ggattgttgc taagaaagtc aacgagattc tacaagcaga
     5401 cgcaatcaat gggaccgata acgaagtagt taccgtgacc gatgagaaca ctggtgaaat
     5461 ctctgagaaa gtcaagctgg gcactaaggc actggctggt caatggctgg ctcacggtgt
     5521 tactcgcagt gtgactaagc gttcagtcat gacgctggct tacgggtcca aagagttcgg
     5581 cttccgtcaa caagtgctgg aagataccat tcagccagct attgattccg gcaagggtcc
     5641 gatgttcact cagccgaatc aggctgctgg atacatggct aagctgattt gggaatctgt
     5701 gagcgtgacg gtggtagctg cggttgaagc aatgaactgg cttaagtctg ctgctaagct
     5761 gctggctgct gaggtcaaag ataagaagac tggagagatt cttcgcaagc gttgcgctgt
     5821 gcattgggta actcctgatg gtttccctgt gtggcaggaa tacaagaagc ctattcagac
     5881 gcgcttgaac ctgatgttcc tcggtcagtt ccgcttacag cctaccatta acaccaacaa
     5941 agatagcgag attgatgcac acaaacagga gtctggtatc gctcctaact ttgtacacag
     6001 ccaagacggt agccaccttc gtaagactgt agtgtgggca cacgagaagt acggaatcga
     6061 atcttttgca ctgattcacg actccttcgg taccattccg gctgacgctg cgaacctgtt
     6121 caaagcagtg cgcgaaacta tggttgacac atatgagtct tgtgatgtac tggctgattt
     6181 ctacgaccag ttcgctgacc agttgcacga gtctcaattg gacaaaatgc cagcacttcc
     6241 ggctaaaggt aacttgaacc tccgtgacat cttagagtcg gacttcgcgt tcgcgtaacg
     6301 ccaaatcaat acgactccgg atcctctacg ccggacgcat cgtggccggc atcaccggcg
     6361 ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc
     6421 gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg
     6481 ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg
     6541 gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgac
     6601 cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta
     6661 tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag
     6721 cgctctgggt cattttcggc gaggaccgct ttcgctggag cgcgacgatg atcggcctgt
     6781 cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca
     6841 ccaaacgttt cggcgagaag caggccatta tcgccggcat ggcggccgac gcgctgggct
     6901 acgtcttgct ggcgttcgcg acgcgaggct ggatggcctt ccccattatg attcttctcg
     6961 cttccggcgg catcgggatg cccgcgttgc aggccatgct gtccaggcag gtagatgacg
     7021 accatcaggg acagcttcaa ggatcgctcg cggctcttac cagcctaact tcgatcactg
     7081 gaccgctgat cgtcacggcg atttatgccg cctcggcgag cacatggaac gggttggcat
     7141 ggattgtagg cgccgcccta taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga
     7201 gccgggccac ctcgacctga atggaagccg gcggcacctc gctaacggat tcaccactcc
     7261 aagaattgga gccaatcaat tcttgcggag aactgtgaat gcgcaaacca acccttggca
     7321 gaacatatcc atcgcgtccg ccatctccag cagccgcacg cggcgcatct cgggcagcgt
     7381 tgggtcctgg ccacgggtgc gcatgatcgt gctcctgtcg ttgaggaccc ggctaggctg
     7441 gcggggttgc cttactggtt agcagaatga atcaccgata cgcgagcgaa cgtgaagcga
     7501 ctgctgctgc aaaacgtctg cgacctgagc aacaacatga atggtcttcg gtttccgtgt
     7561 ttcgtaaagt ctggaaacgc ggaagtcccc tacgtgctgc tgaagttgcc cgcaacagag
     7621 agtggaacca accggtgata ccacgatact atgactgaga gtcaacgcca tgagcggcct
     7681 catttcttat tctgagttac aacagtccgc accgctgtcc ggtagctcct tccggtgggc
     7741 gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg
     7801 acaggtgccg gcagcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc
     7861 gaaacaagcg ccctgcacca ttatgttccg gatctgcatc gcaggatgct gctggctacc
     7921 ctgtggaaca cctacatctg tattaacgaa gcgctaaccg tttttatcag gctctgggag
     7981 gcagaataaa tgatcatatc gtcaattatt acctccacgg ggagagcctg agcaaactgg
     8041 cctcaggcat ttgagaagca cacggtcaca ctgcttccgg tagtcaataa accggtaaac
     8101 cagcaataga cataagcggc tatttaacga ccctgccctg aaccgacgac cgggtcgaat
     8161 ttgctttcga atttctgcca ttcatccgct tattatcact tattcaggcg tagcaccagg
     8221 cgtttaaggg caccaataac tgccttaaaa aaattacgcc ccgccctgcc actcatcgca
     8281 gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaga cggcatgatg
     8341 aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt
     8401 gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact
     8461 cacccaggga ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc
     8521 caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc
     8581 gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta
     8641 acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca tacg
//
