LOCUS       40924_47463        6037 bp DNA     circular SYN 11-SEP-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6037)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6037)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6037
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     15..31
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    810..950
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1136..1724)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1898..2755)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2756..2860)
                     /label=AmpR promoter
     promoter        2966..3067
                     /label=TRP1 promoter
     CDS             3068..3739
                     /codon_start=1
                     /label=TRP1
                     /note="phosphoribosylanthranilate isomerase, required for 
                     tryptophan biosynthesis"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(4103..5445)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     terminator      complement(5686..5933)
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
ORIGIN
        1 accgggcccc ccctcgaggt cgacggtatc gataagcttg atatcgaatt cctgcagccc
       61 gggggatcct ctagagtcga gcttttgtgt attacgatat agttaatagt tgatagttga
      121 ttgtatgctt tttgtagctt gatattctat ttaccaagaa gaaacaagaa gtgataaaaa
      181 caacaagaga gcagtagtaa gagtatttcg agtgtgaaaa aagtcgctac tggcactcta
      241 tttatatgtg ataggcatgc tatagcttta ccaaaaagtg aaccccattt ctatgctctc
      301 ctctgccttt tttttttttt tttttttcat tctctcaatc tgaaattctc ttatttctcc
      361 aacttataag ttggagatgc ccggtgttcc ggcagaggag atcagtctcg tgaagtggat
      421 ggtttcccgc ctgcgggcaa aacgtcataa catttttatg agcgaaagcc gttaatgaag
      481 acaaaatccc ttaattaaaa cattagaatg gtgattagaa aggcaggatt aatcagttac
      541 acaggctgta accggagaga cggatcataa ggcaattttt agataagact ggttagagtt
      601 cttggcatca gaaaatttga gaaacgattt ttccgtttgt ttgcccctac gttttgcccc
      661 tttgatctct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
      721 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
      781 gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc
      841 ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
      901 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg
      961 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     1021 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     1081 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     1141 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     1201 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     1261 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     1321 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     1381 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     1441 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     1501 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     1561 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     1621 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     1681 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     1741 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     1801 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     1861 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     1921 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     1981 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     2041 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     2101 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     2161 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     2221 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     2281 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     2341 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     2401 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     2461 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     2521 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     2581 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     2641 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     2701 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     2761 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     2821 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     2881 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     2941 cgaggccctt tcgtcttcaa gaattaattc ggtcgaaaaa agaaaaggag agggccaaga
     3001 gggagggcat tggtgactat tgagcacgtg agtatacgtg attaagcaca caaaggcagc
     3061 ttggagtatg tctgttatta atttcacagg tagttctggt ccattggtga aagtttgcgg
     3121 cttgcagagc acagaggccg cagaatgtgc tctagattcc gatgctgact tgctgggtat
     3181 tatatgtgtg cccaatagaa agagaacaat tgacccggtt attgcaagga aaatttcaag
     3241 tcttgtaaaa gcatataaaa atagttcagg cactccgaaa tacttggttg gcgtgtttcg
     3301 taatcaacct aaggaggatg ttttggctct ggtcaatgat tacggcattg atatcgtcca
     3361 actgcatgga gatgagtcgt ggcaagaata ccaagagttc ctcggtttgc cagttattaa
     3421 aagactcgta tttccaaaag actgcaacat actactcagt gcagcttcac agaaacctca
     3481 ttcgtttatt cccttgtttg attcagaagc aggtgggaca ggtgaacttt tggattggaa
     3541 ctcgatttct gactgggttg gaaggcaaga gagccccgaa agcttacatt ttatgttagc
     3601 tggtggactg acgccagaaa atgttggtga tgcgcttaga ttaaatggcg ttattggtgt
     3661 tgatgtaagc ggaggtgtgg agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt
     3721 cgtcaaaaat gctaagaaat aggttattac tgagtagtat ttatttaagt attgtttgtg
     3781 cacttgcctg cagcttctca atgatattcg aatacgcttt gaggagatac agcctaatat
     3841 ccgacaaact gttttacaga tttacgatcg tacttgttac ccatcattga attttgaaca
     3901 tccgaacctg ggagttttcc ctgaaacaga tagtatattt gaacctgtat aataatatat
     3961 agtctagcgc tttacggaag acaatgtatg tatttcggtt cctggagaaa ctattgcatc
     4021 tattgcatag gtaatcttgc acgtcgcatc cccggttcat tttctgcgtt tccatcttgc
     4081 acttcaatag catatctttg ttaacgaagc atctgtgctt cattttgtag aacaaaaatg
     4141 caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa
     4201 atgcaacgcg aaagcgctat tttaccaacg aagaatctgt gcttcatttt tgtaaaacaa
     4261 aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa
     4321 cagaaatgca acgcgagagc gctattttac caacaaagaa tctatacttc ttttttgttc
     4381 tacaaaaatg catcccgaga gcgctatttt tctaacaaag catcttagat tacttttttt
     4441 ctcctttgtg cgctctataa tgcagtctct tgataacttt ttgcactgta ggtccgttaa
     4501 ggttagaaga aggctacttt ggtgtctatt ttctcttcca taaaaaaagc ctgactccac
     4561 ttcccgcgtt tactgattac tagcgaagct gcgggtgcat tttttcaaga taaaggcatc
     4621 cccgattata ttctataccg atgtggattg cgcatacttt gtgaacagaa agtgatagcg
     4681 ttgatgattc ttcattggtc agaaaattat gaacggtttc ttctattttg tctctatata
     4741 ctacgtatag gaaatgttta cattttcgta ttgttttcga ttcactctat gaatagttct
     4801 tactacaatt tttttgtcta aagagtaata ctagagataa acataaaaaa tgtagaggtc
     4861 gagtttagat gcaagttcaa ggagcgaaag gtggatgggt aggttatata gggatatagc
     4921 acagagatat atagcaaaga gatacttttg agcaatgttt gtggaagcgg tattcgcaat
     4981 attttagtag ctcgttacag tccggtgcgt ttttggtttt ttgaaagtgc gtcttcagag
     5041 cgcttttggt tttcaaaagc gctctgaagt tcctatactt tctagagaat aggaacttcg
     5101 gaataggaac ttcaaagcgt ttccgaaaac gagcgcttcc gaaaatgcaa cgcgagctgc
     5161 gcacatacag ctcactgttc acgtcgcacc tatatctgcg tgttgcctgt atatatatat
     5221 acatgagaag aacggcatag tgcgtgttta tgcttaaatg cgtacttata tgcgtctatt
     5281 tatgtaggat gaaaggtagt ctagtacctc ctgtgatatt atcccattcc atgcggggta
     5341 tcgtatgctt ccttcagcac taccctttag ctgttctata tgctgccact cctcaattgg
     5401 attagtctca tccttcaatg ctatcatttc ctttgatatt ggatcatatg catagtaccg
     5461 agaaactagt gcgaagtagt gatcaggtat tgctgttatc tgatgagtat acgttgtcct
     5521 ggccacggca gaagcacgct tatcgctcca atttcccaca acattagtca actccgttag
     5581 gcccttcatt gaaagaaatg aggtcatcaa atgtcttcca atgtgagatt ttgggccatt
     5641 ttttatagca aagattgaat aaggcgcatt tttcttcaaa gctttgcaaa ttaaagcctt
     5701 cgagcgtccc aaaaccttct caagcaaggt tttcagtata atgttacatg cgtacacgcg
     5761 tctgtacaga aaaaaaagaa aaatttgaaa tataaataac gttcttaata ctaacataac
     5821 tataaaaaaa taaataggga cctagacttc aggttgtcta actccttcct tttcggttag
     5881 agcggatgtg gggggagggc gtgaatgtaa gcgtgacata actaattaca tgatatcgac
     5941 aaaggaaaag gggcctgttt actcacaggc ttttttcaag taggtaatta agtcgtttct
     6001 gtctttttcc ttcttcaacc caccaaaggc catcttg
//
