LOCUS       40924_47458        6236 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pYAA-ZP-MCS, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6236)
  AUTHORS   Zaltsman AA.
  TITLE     pYAA-ZP-MCS: plasmid for expression, localization, and tagging in 
            yeast with an adenine biosynthesis reporter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6236)
  AUTHORS   Zaltsman AA.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUL-2008) Biochemistry and Cell Biology, State 
            University of New York, Stony Brook, NY 11794, USA
REFERENCE   3  (bases 1 to 6236)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6236)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-JUL-2008) Biochemistry and Cell Biology, State University of New
            York, Stony Brook, NY 11794, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6236
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        199..574
                     /label=ADE2 promoter
     CDS             575..2287
                     /label=ADE2
                     /note="phosphoribosylaminoimidazole carboxylase, required
                     for adenine biosynthesis"
     rep_origin      complement(2495..2950)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     3095..3111
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3121..3139
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    3241..3396
                     /note="multiple cloning site containing ISCEI, PIPSPI,
                     ICEUI, and IPPOI unique sites"
     primer_bind     complement(3410..3426)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(3463..3481)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3502..3518)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3526..3542)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3550..3580)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3595..3616)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3904..4492)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4666..5523)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5524..5628)
                     /label=AmpR promoter
     misc_feature    5664..6167
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatagatc tgaattaatt cttgaataat acataacttt tcttaaaaga atcaaagaca
      241 gataaaattt aagagatatt aaatattagt gagaagccga gaattttgta acaccaacat
      301 aacactgaca tctttaacaa cttttaatta tgatacattt cttacgtcat gattgattat
      361 tacagctatg ctgacaaatg actcttgttg catggctacg aaccgggtaa tactaagtga
      421 ttgactcttg ctgacctttt attaagaact aaatggacaa tattatggag catttcatgt
      481 ataaattggt gcgtaaaatc gttggatctc tcttctaagt acatcctact ataacaatca
      541 agaaaaacaa gaaaatcgga caaaacaatc aagtatggat tctagaacag ttggtatatt
      601 aggaggggga caattgggac gtatgattgt tgaggcagca aacaggctca acattaagac
      661 ggtaatacta gatgctgaaa attctcctgc caaacaaata agcaactcca atgaccacgt
      721 taatggctcc ttttccaatc ctcttgatat cgaaaaacta gctgaaaaat gtgatgtgct
      781 aacgattgag attgagcatg ttgatgttcc tacactaaag aatcttcaag taaaacatcc
      841 caaattaaaa atttaccctt ctccagaaac aatcagattg atacaagaca aatatattca
      901 aaaagagcat ttaatcaaaa atggtatagc agttacccaa agtgttcctg tggaacaagc
      961 cagtgagacg tccctattga atgttggaag agatttgggt tttccattcg tcttgaagtc
     1021 gaggactttg gcatacgatg gaagaggtaa cttcgttgta aagaataagg aaatgattcc
     1081 ggaagctttg gaagtactga aggatcgtcc tttgtacgcc gaaaaatggg caccatttac
     1141 taaagaatta gcagtcatga ttgtgaggtc tgttaacggt ttagtgtttt cttacccaat
     1201 tgtagagact atccacaagg acaatatttg tgacttatgt tatgcgcctg ctagagttcc
     1261 ggactccgtt caacttaagg cgaagttgtt ggcagaaaat gcaatcaaat cttttcccgg
     1321 ttgtggtata tttggtgtgg aaatgttcta tttagaaaca ggggaattgc ttattaacga
     1381 aattgcccca aggcctcaca actctggaca ttataccatt gatgcttgcg tcacttctca
     1441 atttgaagct catttgagat caatattgga tttgccaatg ccaaagaatt tcacatcttt
     1501 ctccaccatt acaacgaacg ccattatgct aaatgttctt ggagacaaac atacaaaaga
     1561 taaagagcta gaaacttgcg aaagagcatt ggcgactcca ggttcctcag tgtacttata
     1621 tggaaaagag tctagaccta acagaaaagt aggtcacata aatattattg cctccagtat
     1681 ggcggaatgt gaacaaaggc tgaactacat tacaggtaga actgatattc caatcaaaat
     1741 ctctgtcgct caaaagttgg acttggaagc aatggtcaaa ccattggttg gaatcatcat
     1801 gggatcagac tctgacttgc cggtaatgtc tgccgcatgt gcggttttaa aagattttgg
     1861 cgttccattt gaagtgacaa tagtctctgc tcatagaact ccacatagga tgtcagcata
     1921 tgctatttcc gcaagcaagc gtggaattaa aacaattatc gctggagctg gtggggctgc
     1981 tcacttgcca ggtatggtgg ctgcaatgac accacttcct gtcatcggtg tgcccgtaaa
     2041 aggttcttgt ctagatggag tagattcttt acattcaatt gtgcaaatgc ctagaggtgt
     2101 tccagtagct accgtcgcta ttaataatag tacgaacgct gcgctgttgg ctgtcagact
     2161 gcttggcgct tatgattcaa gttatacaac gaaaatggaa cagtttttat taaagcaaga
     2221 agaagaagtt cttgtcaaag cacaaaagtt agaaactgtc ggttacgaag cttatctaga
     2281 aaacaagtaa tatataagtt tattgatata cttgtacagc aaataattat aaaatgatat
     2341 acctattttt taggctttgt tatgattaca tcaaatgtgg acttcataca tagaaatcaa
     2401 cgcttacagg tgtccttttt taagaatttc atacataaga tctatgcggt gtgaaatacc
     2461 gcacagatgc gtaaggagaa aataccgcat caggaaattg taaacgttaa tattttgtta
     2521 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     2581 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     2641 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     2701 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     2761 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     2821 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     2881 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     2941 cagggcgcgt cgcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     3001 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     3061 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg
     3121 taatacgact cactataggg cgaattgggt accgggcccc ccctcgaggt cgacgcccgg
     3181 gcggtaccgc gatcgctcgc gacctgcagg cataaagccg tcagtgtccg cataaagaac
     3241 cacccataat acccataata gctgtttgcc atcgctacct taggaccgtt atagttaatt
     3301 accctgttat ccctattaat taagagctcg ctaccttaag agaggatatc ggcgcgcctc
     3361 tagaatttaa atggatccta cgtactcgag gaattcctgc agcccggggg atccactagt
     3421 tctagagcgg ccgccaccgc ggtggagctc cagcttttgt tccctttagt gagggttaat
     3481 tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     3541 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     3601 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     3661 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     3721 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     3781 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     3841 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     3901 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     3961 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     4021 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     4081 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     4141 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     4201 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     4261 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     4321 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     4381 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     4441 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     4501 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     4561 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     4621 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     4681 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     4741 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     4801 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     4861 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     4921 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     4981 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     5041 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     5101 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     5161 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     5221 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     5281 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     5341 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     5401 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     5461 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     5521 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     5581 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     5641 aaaagtgcca cctggtcctt ttcatcacgt gctataaaaa taattataat ttaaattttt
     5701 taatataaat atataaatta aaaatagaaa gtaaaaaaag aaattaaaga aaaaatagtt
     5761 tttgttttcc gaagatgtaa aagactctag ggggatcgcc aacaaatact accttttatc
     5821 ttgctcttcc tgctctcagg tattaatgcc gaattgtttc atcttgtctg tgtagaagac
     5881 cacacacgaa aatcctgtga ttttacattt tacttatcgt taatcgaatg tatatctatt
     5941 taatctgctt ttcttgtcta ataaatatat atgtaaagta cgctttttgt tgaaattttt
     6001 taaacctttg tttatttttt tttcttcatt ccgtaactct tctaccttct ttatttactt
     6061 tctaaaatcc aaatacaaaa cataaaaata aataaacaca gagtaaattc ccaaattatt
     6121 ccatcattaa aagatacgag gcgcgtgtaa gttacaggca agcgatccgt cctaagaaac
     6181 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc
//
