LOCUS       62056_22950        8952 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8952)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8952
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      69..1411
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        1438..1542
                     /label=AmpR promoter
     CDS             1543..2400
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2574..3162
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3450..3471
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3486..3516
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3524..3540
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3548..3564
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3585..3603
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     terminator      complement(3643..3807)
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             complement(4536..4799)
                     /codon_start=1
                     /label=alpha-factor secretion signal
                     /note="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
                     /translation="MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYSDLE
                     GDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLEKREAE"
     promoter        complement(5240..5258)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5278..5921
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate
                     dehydrogenase; also known as the TDH3 promoter"
     CDS             5939..6202
                     /codon_start=1
                     /label=alpha-factor secretion signal
                     /note="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
                     /translation="MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYSDLE
                     GDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLEKREAE"
     primer_bind     complement(6752..6768)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      6771..7018
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     promoter        complement(7037..7055)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7065..7081)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      7226..7681
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        complement(8211..8618)
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagtatga tccaatatca aaggaaatga tagcattgaa ggatgagact aatccaattg
      121 aggagtggca gcatatagaa cagctaaagg gtagtgctga aggaagcata cgataccccg
      181 catggaatgg gataatatca caggaggtac tagactacct ttcatcctac ataaatagac
      241 gcatataagt acgcatttaa gcataaacac gcactatgcc gttcttctca tgtatatata
      301 tatacaggca acacgcagat ataggtgcga cgtgaacagt gagctgtatg tgcgcagctc
      361 gcgttgcatt ttcggaagcg ctcgttttcg gaaacgcttt gaagttccta ttccgaagtt
      421 cctattctct agaaagtata ggaacttcag agcgcttttg aaaaccaaaa gcgctctgaa
      481 gacgcacttt caaaaaacca aaaacgcacc ggactgtaac gagctactaa aatattgcga
      541 ataccgcttc cacaaacatt gctcaaaagt atctctttgc tatatatctc tgtgctatat
      601 ccctatataa cctacccatc cacctttcgc tccttgaact tgcatctaaa ctcgacctct
      661 acatttttta tgtttatctc tagtattact ctttagacaa aaaaattgta gtaagaacta
      721 ttcatagagt gaatcgaaaa caatacgaaa atgtaaacat ttcctatacg tagtatatag
      781 agacaaaata gaagaaaccg ttcataattt tctgaccaat gaagaatcat caacgctatc
      841 actttctgtt cacaaagtat gcgcaatcca catcggtata gaatataatc ggggatgcct
      901 ttatcttgaa aaaatgcacc cgcagcttcg ctagtaatca gtaaacgcgg gaagtggagt
      961 caggcttttt ttatggaaga gaaaatagac accaaagtag ccttcttcta accttaacgg
     1021 acctacagtg caaaaagtta tcaagagact gcattataga gcgcacaaag gagaaaaaaa
     1081 gtaatctaag atgctttgtt agaaaaatag cgctctcggg atgcattttt gtagaacaaa
     1141 aaagaagtat agattctttg ttggtaaaat agcgctctcg cgttgcattt ctgttctgta
     1201 aaaatgcagc tcagattctt tgtttgaaaa attagcgctc tcgcgttgca tttttgtttt
     1261 acaaaaatga agcacagatt cttcgttggt aaaatagcgc tttcgcgttg catttctgtt
     1321 ctgtaaaaat gcagctcaga ttctttgttt gaaaaattag cgctctcgcg ttgcattttt
     1381 gttctacaaa atgaagcaca gatgcttcgt tcaggtggca cttttcgggg aaatgtgcgc
     1441 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     1501 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     1561 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     1621 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     1681 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     1741 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     1801 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     1861 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     1921 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     1981 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     2041 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     2101 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     2161 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     2221 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     2281 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     2341 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     2401 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     2461 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     2521 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     2581 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     2641 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     2701 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     2761 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     2821 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     2881 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2941 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     3001 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     3061 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     3121 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     3181 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     3241 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     3301 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     3361 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     3421 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt acctcactca ttaggcaccc
     3481 caggctttac actttatgct tccggctcct atgttgtgtg gaattgtgag cggataacaa
     3541 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
     3601 agggaacaaa agctggagct cgctattacg ccagctgaat tggagcgacc tcatgctata
     3661 ctgagaaagc aacctgacct acaggaaaga gttactcaag aataagaatt ttcgttttaa
     3721 aacctaagag tcactttaaa atttgtatac acttattttt tttataactt atttaataat
     3781 aaaaatcata aatcataaga aattcgctta tttagaagtg tcaacaacgt atctaccaac
     3841 gatttgaccc ttttccatct tttcgtaaat ttctggcaag gtagacaagc cgacaacctt
     3901 gattggagac ttgaccaaac ctctggcgaa gaattgttaa ttaaagatct ccgcggttat
     3961 ggcttcaatg gtgggtcctt caagttgaat tctctgaatt gaacgtacat agtgattctg
     4021 atgttgtagt cttggtcgta gatagagttt tcgaaagcag tacccaaacc aacgtggtca
     4081 acgttaccag tagtttgcaa tctcaaccac aattggtttc tcttgttgtt tggttggaag
     4141 tagtcgatag ttctgtccaa aactggcttt ggagtgaagt atctagagtg gtagtagaat
     4201 ggttgagtga tagtgtgtct agaagagtag ttaacgtatg ggtcgtaagt caaagcgtta
     4261 gccttagtaa cgaagttgtc gtccaagata acagcagtag aaccaacacc tctgtcacct
     4321 tgagtgattg gagaacatgg ccagaattca accttaacct ttctgattct gtagtattcg
     4381 aatggaacag tcaatgggtt agaaccacca cctggtggca agaagtcgtt gatgttgaat
     4441 ctcatcatgt caacgttcca agatggagtt ctaacagtag tcttcttaac agtgtaaccg
     4501 atagttctag acaatctagt gttgaagata ccgttttcag cctctctctt ctcgagagat
     4561 accccttctt ctttagcagc aatgctggca atagtagtat ttataaacaa taacccgtta
     4621 tttgtgctgt tggaaaatgg caaaacagca acatcgaaat ccccttctaa atctgagtaa
     4681 ccgatgacag cttcagccgg aatttgtgcc gtttcatctt ctgttgtagt gttgactgga
     4741 gcagctaatg cggaggatgc tgcgaataaa acagcagtaa aaattgaagg aaatctcata
     4801 gcggccgcgc tagttctaga aaacttagat tagattgcta tgctttcttt ctaatgagca
     4861 agaagtaaaa aaagttgtaa tagaacaaga aaaatgaaac tgaaacttga gaaattgaag
     4921 accgtttatt aacttaaata tcaatgggag gtcatcgaaa gagaaaaaaa tcaaaaaaaa
     4981 aattttcaag aaaaagaaac gtgataaaaa tttttattgc ctttttcgac gaagaaaaag
     5041 aaacgaggcg gtctcttttt tcttttccaa acctttagta cgggtaatta acgacaccct
     5101 agaggaagaa agaggggaaa tttagtatgc tgtgcttggg tgttttgaag tggtacggcg
     5161 atgcgcggag tccgagaaaa tctggaagag taaaaaagga gtagaaacat tttgaagcta
     5221 tgagctccag cttttgttcc ctttagtgag ggttaattgc gcgccgagct cagtttatca
     5281 ttatcaatac tcgccatttc aaagaatacg taaataatta atagtagtga ttttcctaac
     5341 tttatttagt caaaaaatta gccttttaat tctgctgtaa cccgtacatg cccaaaatag
     5401 ggggcgggtt acacagaata tataacatcg taggtgtctg ggtgaacagt ttattcctgg
     5461 catccactaa atataatgga gcccgctttt taagctggca tccagaaaaa aaaagaatcc
     5521 cagcaccaaa atattgtttt cttcaccaac catcagttca taggtccatt ctcttagcgc
     5581 aactacagag aacaggggca caaacaggca aaaaacgggc acaacctcaa tggagtgatg
     5641 caacctgcct ggagtaaatg atgacacaag gcaattgacc cacgcatgta tctatctcat
     5701 tttcttacac cttctattac cttctgctct ctctgatttg gaaaaagctg aaaaaaaagg
     5761 ttgaaaccag ttccctgaaa ttattcccct acttgactaa taagtatata aagacggtag
     5821 gtattgattg taattctgta aatctatttc ttaaacttct taaattctac ttttatagtt
     5881 agtctttttt ttagttttaa aacaccagaa cttagtttcg acggattcta gaactagtat
     5941 gagatttcct tcaattttta ctgctgtttt attcgcagca tcctccgcat tagctgctcc
     6001 agtcaacact acaacagaag atgaaacggc acaaattccg gctgaagctg tcatcggtta
     6061 ctcagattta gaaggggatt tcgatgttgc tgttttgcca ttttccaaca gcacaaataa
     6121 cgggttattg tttataaata ctactattgc cagcattgct gctaaagaag aaggggtatc
     6181 tctcgagaag agagaggctg aaccaactgc tggtacttac tacactaaga agtactctac
     6241 tatgaacgtt atctctgttg gtactccaca aaacaacaag ccatggcacg ctaaccactt
     6301 catcactaga ttgaacgaat gggaaactgc tatcactttc gaatactaca agatcttgaa
     6361 gatgaaggtt actttgtctc cagttatctc tccagctcaa caaactaaga ctatgttcgg
     6421 tcacactgct atcgacttgg acggtgcttg gactactaac acttggttgc aagacgaccc
     6481 atacgctgaa tcttctacta gaaaggttat gacttctaag aagaagcact ctagatactt
     6541 cactccaaag ccattgttgg ctggtactac ttctgctcac ccaggtcaat ctttgttctt
     6601 cttctctaga ccaactccat ggttgaacac ttacgaccca actgttcaat ggggtgcttt
     6661 gttgtggtct atctacgttc cagaaaagac tggtatgact gacttctacg gtactaagga
     6721 agtttggatc agatacaagt cttaagctta tcgataccgt cgacctcgag tcatgtaatt
     6781 agttatgtca cgcttacatt cacgccctcc ccccacatcc gctctaaccg aaaaggaagg
     6841 agttagacaa cctgaagtct aggtccctat ttattttttt atagttatgt tagtattaag
     6901 aacgttattt atatttcaaa tttttctttt ttttctgtac agacgcgtgt acgcatgtaa
     6961 cattatactg aaaaccttgc ttgagaaggt tttgggacgc tcgaaggctt taatttgcgg
     7021 ccggtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     7081 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     7141 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     7201 cgcagcctga atggcgaatg gcgcgacgcg ccctgtagcg gcgcattaag cgcggcgggt
     7261 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     7321 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     7381 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     7441 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     7501 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     7561 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     7621 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt
     7681 tcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atagggtaat
     7741 aactgatata attaaattga agctctaatt tgtgagttta gtatacatgc atttacttat
     7801 aatacagttt ttcatgagat gcctgcaagc aattcgttct gtatcaggcg caggagcgtc
     7861 ccgtccgggt cgaccaaagc ggccatcgtg cctccccact cctgcagttc gggggcatgg
     7921 atgcgcggat agccgctgct ggtttcctgg atgccgacgg atttgcactg ccggtagaac
     7981 tccgcgaggt cgtccagcct caggcagcag ctgaaccaac tcgcgagggg atcgagcccg
     8041 gggtgggcga agaactccag catgagatcc ccgcgctgga ggatcatcca gccggcgtcc
     8101 cggaaaacga ttccgaagcc caacctttca tagaaggcgg cggtggaatc gaaatctcgt
     8161 gatggcaggt tgggcgtcgc ttggtcggtc atttcgaatt cgagctcgcc cttagattag
     8221 attgctatgc tttctttcta atgagcaaga agtaaaaaaa gttgtaatag aacaagaaaa
     8281 atgaaactga aacttgagaa attgaagacc gtttattaac ttaaatatca atgggaggtc
     8341 atcgaaagag aaaaaaatca aaaaaaaaaa ttttcaagaa aaagaaacgt gataaaaatt
     8401 tttattgcct ttttcgacga agaaaaagaa acgaggcggt ctcttttttc ttttccaaac
     8461 ctttagtacg ggtaattaac gacaccctag aggaagaaag aggggaaatt tagtatgctg
     8521 tgcttgggtg ttttgaagtg gtacggcgat gcgcggagtc cgagaaaatc tggaagagta
     8581 aaaaaggagt agaaacattt tgaagctatg gtgtgtggca tatgcgtata tataccaatc
     8641 taagtctgtg ctccttcctt cgttcttcct tctgttcgga gattaccgaa tcaaaaaaat
     8701 ttcaaagaaa ccgaaatcaa aaaaaagaat aaaaaaaaaa tgatgaattg aattgaaaag
     8761 ctgtggtatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     8821 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     8881 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     8941 cgaaacgcgc ga
//
