LOCUS       62056_22935        7351 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7351)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7351
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      69..1411
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        1438..1542
                     /label=AmpR promoter
     CDS             1543..2400
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2574..3162
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3450..3471
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3486..3516
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3524..3540
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3548..3564
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3585..3603
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     terminator      complement(3643..3807)
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     promoter        complement(4414..4432)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4452..5095
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     5101..5117
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5151..5167)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      5170..5417
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     promoter        complement(5436..5454)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5464..5480)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      5625..6080
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        complement(6610..7017)
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagtatga tccaatatca aaggaaatga tagcattgaa ggatgagact aatccaattg
      121 aggagtggca gcatatagaa cagctaaagg gtagtgctga aggaagcata cgataccccg
      181 catggaatgg gataatatca caggaggtac tagactacct ttcatcctac ataaatagac
      241 gcatataagt acgcatttaa gcataaacac gcactatgcc gttcttctca tgtatatata
      301 tatacaggca acacgcagat ataggtgcga cgtgaacagt gagctgtatg tgcgcagctc
      361 gcgttgcatt ttcggaagcg ctcgttttcg gaaacgcttt gaagttccta ttccgaagtt
      421 cctattctct agaaagtata ggaacttcag agcgcttttg aaaaccaaaa gcgctctgaa
      481 gacgcacttt caaaaaacca aaaacgcacc ggactgtaac gagctactaa aatattgcga
      541 ataccgcttc cacaaacatt gctcaaaagt atctctttgc tatatatctc tgtgctatat
      601 ccctatataa cctacccatc cacctttcgc tccttgaact tgcatctaaa ctcgacctct
      661 acatttttta tgtttatctc tagtattact ctttagacaa aaaaattgta gtaagaacta
      721 ttcatagagt gaatcgaaaa caatacgaaa atgtaaacat ttcctatacg tagtatatag
      781 agacaaaata gaagaaaccg ttcataattt tctgaccaat gaagaatcat caacgctatc
      841 actttctgtt cacaaagtat gcgcaatcca catcggtata gaatataatc ggggatgcct
      901 ttatcttgaa aaaatgcacc cgcagcttcg ctagtaatca gtaaacgcgg gaagtggagt
      961 caggcttttt ttatggaaga gaaaatagac accaaagtag ccttcttcta accttaacgg
     1021 acctacagtg caaaaagtta tcaagagact gcattataga gcgcacaaag gagaaaaaaa
     1081 gtaatctaag atgctttgtt agaaaaatag cgctctcggg atgcattttt gtagaacaaa
     1141 aaagaagtat agattctttg ttggtaaaat agcgctctcg cgttgcattt ctgttctgta
     1201 aaaatgcagc tcagattctt tgtttgaaaa attagcgctc tcgcgttgca tttttgtttt
     1261 acaaaaatga agcacagatt cttcgttggt aaaatagcgc tttcgcgttg catttctgtt
     1321 ctgtaaaaat gcagctcaga ttctttgttt gaaaaattag cgctctcgcg ttgcattttt
     1381 gttctacaaa atgaagcaca gatgcttcgt tcaggtggca cttttcgggg aaatgtgcgc
     1441 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     1501 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     1561 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     1621 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     1681 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     1741 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     1801 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     1861 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     1921 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     1981 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     2041 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     2101 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     2161 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     2221 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     2281 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     2341 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     2401 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     2461 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     2521 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     2581 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     2641 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     2701 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     2761 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     2821 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     2881 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2941 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     3001 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     3061 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     3121 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     3181 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     3241 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     3301 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     3361 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     3421 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt acctcactca ttaggcaccc
     3481 caggctttac actttatgct tccggctcct atgttgtgtg gaattgtgag cggataacaa
     3541 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
     3601 agggaacaaa agctggagct cgctattacg ccagctgaat tggagcgacc tcatgctata
     3661 ctgagaaagc aacctgacct acaggaaaga gttactcaag aataagaatt ttcgttttaa
     3721 aacctaagag tcactttaaa atttgtatac acttattttt tttataactt atttaataat
     3781 aaaaatcata aatcataaga aattcgctta tttagaagtg tcaacaacgt atctaccaac
     3841 gatttgaccc ttttccatct tttcgtaaat ttctggcaag gtagacaagc cgacaacctt
     3901 gattggagac ttgaccaaac ctctggcgaa gaattgttaa ttaaagatct ccgcggtccg
     3961 gagttaactg atcagcggcc gcgctagttc tagaaaactt agattagatt gctatgcttt
     4021 ctttctaatg agcaagaagt aaaaaaagtt gtaatagaac aagaaaaatg aaactgaaac
     4081 ttgagaaatt gaagaccgtt tattaactta aatatcaatg ggaggtcatc gaaagagaaa
     4141 aaaatcaaaa aaaaaatttt caagaaaaag aaacgtgata aaaattttta ttgccttttt
     4201 cgacgaagaa aaagaaacga ggcggtctct tttttctttt ccaaaccttt agtacgggta
     4261 attaacgaca ccctagagga agaaagaggg gaaatttagt atgctgtgct tgggtgtttt
     4321 gaagtggtac ggcgatgcgc ggagtccgag aaaatctgga agagtaaaaa aggagtagaa
     4381 acattttgaa gctatgagct ccagcttttg ttccctttag tgagggttaa ttgcgcgccg
     4441 agctcagttt atcattatca atactcgcca tttcaaagaa tacgtaaata attaatagta
     4501 gtgattttcc taactttatt tagtcaaaaa attagccttt taattctgct gtaacccgta
     4561 catgcccaaa atagggggcg ggttacacag aatatataac atcgtaggtg tctgggtgaa
     4621 cagtttattc ctggcatcca ctaaatataa tggagcccgc tttttaagct ggcatccaga
     4681 aaaaaaaaga atcccagcac caaaatattg ttttcttcac caaccatcag ttcataggtc
     4741 cattctctta gcgcaactac agagaacagg ggcacaaaca ggcaaaaaac gggcacaacc
     4801 tcaatggagt gatgcaacct gcctggagta aatgatgaca caaggcaatt gacccacgca
     4861 tgtatctatc tcattttctt acaccttcta ttaccttctg ctctctctga tttggaaaaa
     4921 gctgaaaaaa aaggttgaaa ccagttccct gaaattattc ccctacttga ctaataagta
     4981 tataaagacg gtaggtattg attgtaattc tgtaaatcta tttcttaaac ttcttaaatt
     5041 ctacttttat agttagtctt ttttttagtt ttaaaacacc agaacttagt ttcgacggat
     5101 tctagaacta gtggatcccc cgggctgcag gaattcgata tcaagcttat cgataccgtc
     5161 gacctcgagt catgtaatta gttatgtcac gcttacattc acgccctccc cccacatccg
     5221 ctctaaccga aaaggaagga gttagacaac ctgaagtcta ggtccctatt tattttttta
     5281 tagttatgtt agtattaaga acgttattta tatttcaaat ttttcttttt tttctgtaca
     5341 gacgcgtgta cgcatgtaac attatactga aaaccttgct tgagaaggtt ttgggacgct
     5401 cgaaggcttt aatttgcggc cggtacccaa ttcgccctat agtgagtcgt attacgcgcg
     5461 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     5521 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     5581 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcgacgcgc cctgtagcgg
     5641 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     5701 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     5761 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     5821 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     5881 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     5941 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     6001 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     6061 aatattaacg tttacaattt cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     6121 tcacaccgca tagggtaata actgatataa ttaaattgaa gctctaattt gtgagtttag
     6181 tatacatgca tttacttata atacagtttt tcatgagatg cctgcaagca attcgttctg
     6241 tatcaggcgc aggagcgtcc cgtccgggtc gaccaaagcg gccatcgtgc ctccccactc
     6301 ctgcagttcg ggggcatgga tgcgcggata gccgctgctg gtttcctgga tgccgacgga
     6361 tttgcactgc cggtagaact ccgcgaggtc gtccagcctc aggcagcagc tgaaccaact
     6421 cgcgagggga tcgagcccgg ggtgggcgaa gaactccagc atgagatccc cgcgctggag
     6481 gatcatccag ccggcgtccc ggaaaacgat tccgaagccc aacctttcat agaaggcggc
     6541 ggtggaatcg aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca tttcgaattc
     6601 gagctcgccc ttagattaga ttgctatgct ttctttctaa tgagcaagaa gtaaaaaaag
     6661 ttgtaataga acaagaaaaa tgaaactgaa acttgagaaa ttgaagaccg tttattaact
     6721 taaatatcaa tgggaggtca tcgaaagaga aaaaaatcaa aaaaaaaaat tttcaagaaa
     6781 aagaaacgtg ataaaaattt ttattgcctt tttcgacgaa gaaaaagaaa cgaggcggtc
     6841 tcttttttct tttccaaacc tttagtacgg gtaattaacg acaccctaga ggaagaaaga
     6901 ggggaaattt agtatgctgt gcttgggtgt tttgaagtgg tacggcgatg cgcggagtcc
     6961 gagaaaatct ggaagagtaa aaaaggagta gaaacatttt gaagctatgg tgtgtggcat
     7021 atgcgtatat ataccaatct aagtctgtgc tccttccttc gttcttcctt ctgttcggag
     7081 attaccgaat caaaaaaatt tcaaagaaac cgaaatcaaa aaaaagaata aaaaaaaaat
     7141 gatgaattga attgaaaagc tgtggtatgg tgcactctca gtacaatctg ctctgatgcc
     7201 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     7261 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     7321 aggttttcac cgtcatcacc gaaacgcgcg a
//
