LOCUS       62056_22925        7607 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7607)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7607
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      69..1411
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        1438..1542
                     /label=AmpR promoter
     CDS             1543..2400
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2574..3162
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3450..3471
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3486..3516
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3524..3540
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3548..3564
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3585..3603
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     terminator      complement(3643..3807)
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     promoter        complement(3998..4232)
                     /label=CUP1 promoter
                     /note="inducible metallothionein promoter, regulated by
                     Cu2+"
     promoter        complement(4250..4268)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4288..4931
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     4937..4953
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4987..5003)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      5006..5253
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     promoter        complement(5272..5290)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5300..5316)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      5461..5916
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     gene            complement(5917..7273)
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin
                     resistance (Wach et al., 1994)"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagtatga tccaatatca aaggaaatga tagcattgaa ggatgagact aatccaattg
      121 aggagtggca gcatatagaa cagctaaagg gtagtgctga aggaagcata cgataccccg
      181 catggaatgg gataatatca caggaggtac tagactacct ttcatcctac ataaatagac
      241 gcatataagt acgcatttaa gcataaacac gcactatgcc gttcttctca tgtatatata
      301 tatacaggca acacgcagat ataggtgcga cgtgaacagt gagctgtatg tgcgcagctc
      361 gcgttgcatt ttcggaagcg ctcgttttcg gaaacgcttt gaagttccta ttccgaagtt
      421 cctattctct agaaagtata ggaacttcag agcgcttttg aaaaccaaaa gcgctctgaa
      481 gacgcacttt caaaaaacca aaaacgcacc ggactgtaac gagctactaa aatattgcga
      541 ataccgcttc cacaaacatt gctcaaaagt atctctttgc tatatatctc tgtgctatat
      601 ccctatataa cctacccatc cacctttcgc tccttgaact tgcatctaaa ctcgacctct
      661 acatttttta tgtttatctc tagtattact ctttagacaa aaaaattgta gtaagaacta
      721 ttcatagagt gaatcgaaaa caatacgaaa atgtaaacat ttcctatacg tagtatatag
      781 agacaaaata gaagaaaccg ttcataattt tctgaccaat gaagaatcat caacgctatc
      841 actttctgtt cacaaagtat gcgcaatcca catcggtata gaatataatc ggggatgcct
      901 ttatcttgaa aaaatgcacc cgcagcttcg ctagtaatca gtaaacgcgg gaagtggagt
      961 caggcttttt ttatggaaga gaaaatagac accaaagtag ccttcttcta accttaacgg
     1021 acctacagtg caaaaagtta tcaagagact gcattataga gcgcacaaag gagaaaaaaa
     1081 gtaatctaag atgctttgtt agaaaaatag cgctctcggg atgcattttt gtagaacaaa
     1141 aaagaagtat agattctttg ttggtaaaat agcgctctcg cgttgcattt ctgttctgta
     1201 aaaatgcagc tcagattctt tgtttgaaaa attagcgctc tcgcgttgca tttttgtttt
     1261 acaaaaatga agcacagatt cttcgttggt aaaatagcgc tttcgcgttg catttctgtt
     1321 ctgtaaaaat gcagctcaga ttctttgttt gaaaaattag cgctctcgcg ttgcattttt
     1381 gttctacaaa atgaagcaca gatgcttcgt tcaggtggca cttttcgggg aaatgtgcgc
     1441 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     1501 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     1561 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     1621 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     1681 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     1741 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     1801 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     1861 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     1921 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     1981 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     2041 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     2101 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     2161 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     2221 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     2281 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     2341 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     2401 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     2461 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     2521 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     2581 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     2641 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     2701 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     2761 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     2821 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     2881 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2941 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     3001 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     3061 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     3121 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     3181 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     3241 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     3301 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     3361 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     3421 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt acctcactca ttaggcaccc
     3481 caggctttac actttatgct tccggctcct atgttgtgtg gaattgtgag cggataacaa
     3541 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
     3601 agggaacaaa agctggagct cgctattacg ccagctgaat tggagcgacc tcatgctata
     3661 ctgagaaagc aacctgacct acaggaaaga gttactcaag aataagaatt ttcgttttaa
     3721 aacctaagag tcactttaaa atttgtatac acttattttt tttataactt atttaataat
     3781 aaaaatcata aatcataaga aattcgctta tttagaagtg tcaacaacgt atctaccaac
     3841 gatttgaccc ttttccatct tttcgtaaat ttctggcaag gtagacaagc cgacaacctt
     3901 gattggagac ttgaccaaac ctctggcgaa gaattgttaa ttaaagatct ccgcggtccg
     3961 gagttaactg atcagcggcc gcgctagttc tagaaaagat gacttctata tgatattgca
     4021 ctaacaagaa gatattataa tgcaattgat acaagacaag gagttatttg cttctctttt
     4081 atatgattct gacaatccat attgcgttgg tagtcttttt tgctggaacg gttcagcgga
     4141 aaagacgcat cgctcttttt gcttctagaa gaaatgccag caaaagaatc tcttgacagt
     4201 gactgacagc aaaaatgtct ttttctaact agagctccag cttttgttcc ctttagtgag
     4261 ggttaattgc gcgccgagct cagtttatca ttatcaatac tcgccatttc aaagaatacg
     4321 taaataatta atagtagtga ttttcctaac tttatttagt caaaaaatta gccttttaat
     4381 tctgctgtaa cccgtacatg cccaaaatag ggggcgggtt acacagaata tataacatcg
     4441 taggtgtctg ggtgaacagt ttattcctgg catccactaa atataatgga gcccgctttt
     4501 taagctggca tccagaaaaa aaaagaatcc cagcaccaaa atattgtttt cttcaccaac
     4561 catcagttca taggtccatt ctcttagcgc aactacagag aacaggggca caaacaggca
     4621 aaaaacgggc acaacctcaa tggagtgatg caacctgcct ggagtaaatg atgacacaag
     4681 gcaattgacc cacgcatgta tctatctcat tttcttacac cttctattac cttctgctct
     4741 ctctgatttg gaaaaagctg aaaaaaaagg ttgaaaccag ttccctgaaa ttattcccct
     4801 acttgactaa taagtatata aagacggtag gtattgattg taattctgta aatctatttc
     4861 ttaaacttct taaattctac ttttatagtt agtctttttt ttagttttaa aacaccagaa
     4921 cttagtttcg acggattcta gaactagtgg atcccccggg ctgcaggaat tcgatatcaa
     4981 gcttatcgat accgtcgacc tcgagtcatg taattagtta tgtcacgctt acattcacgc
     5041 cctcccccca catccgctct aaccgaaaag gaaggagtta gacaacctga agtctaggtc
     5101 cctatttatt tttttatagt tatgttagta ttaagaacgt tatttatatt tcaaattttt
     5161 cttttttttc tgtacagacg cgtgtacgca tgtaacatta tactgaaaac cttgcttgag
     5221 aaggttttgg gacgctcgaa ggctttaatt tgcggccggt acccaattcg ccctatagtg
     5281 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     5341 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     5401 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcg
     5461 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     5521 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     5581 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     5641 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     5701 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     5761 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     5821 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     5881 acgcgaattt taacaaaata ttaacgttta caatttcagt atagcgacca gcattcacat
     5941 acgattgacg catgatatta ctttctgcgc acttaacttc gcatctgggc agatgatgtc
     6001 gaggcgaaaa aaaatataaa tcacgctaac atttgattaa aatagaacaa ctacaatata
     6061 aaaaaactat acaaatgaca agttcttgaa aacaagaatc tttttattgt cagtactgat
     6121 tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata
     6181 ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat
     6241 aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct
     6301 attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact
     6361 gaatccggtg agaatggcaa aagcttatgc atttctttcc agacttgttc aacaggccag
     6421 ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc
     6481 gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa
     6541 tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat
     6601 tcttctaata cctggaatgc tgttttgccg gggatcgcag tggtgagtaa ccatgcatca
     6661 tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt
     6721 agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac
     6781 aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca
     6841 ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc
     6901 ctcgaaacgt gagtcttttc cttacccatg gttgtttatg ttcggatgtg atgtgagaac
     6961 tgtatcctag caagatttta aaaggaagta tatgaaagaa gaacctcagt ggcaaatcct
     7021 aaccttttat atttctctac aggggcgcgg cgtggggaca attcaacgcg tctgtgaggg
     7081 gagcgtttcc ctgctcgcag gtctgcagcg aggagccgta atttttgctt cgcgccgtgc
     7141 ggccatcaaa atgtatggat gcaaatgatt atacatgggg atgtatgggc taaatgtacg
     7201 ggcgacagtc acatcatgcc cctgagctgc gcacgtcaag actgtcaagg agggtattct
     7261 gggcctccat gtccatatgc gtatatatac caatctaagt ctgtgctcct tccttcgttc
     7321 ttccttctgt tcggagatta ccgaatcaaa aaaatttcaa agaaaccgaa atcaaaaaaa
     7381 agaataaaaa aaaaatgatg aattgaattg aaaagctgtg gtatggtgca ctctcagtac
     7441 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     7501 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     7561 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcga
//
