LOCUS       40924_47443        5106 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pY1-gLUC59(MCS), complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5106)
  AUTHORS   Bonaldi K, Li Z, Kang SE, Breton G, Pruneda-Paz JL.
  TITLE     Novel surface luciferase reporter for high-throughput quantitative 
            test one-hybrid screens
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5106)
  AUTHORS   Pruneda-Paz JL.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2017) Biological Sciences, University of 
            California San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA
REFERENCE   3  (bases 1 to 5106)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5106)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-FEB-2017) Biological Sciences, University of California San 
            Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5106
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..45
                     /label=multiple cloning site
                     /note="multiple cloning site"
     regulatory      46..184
                     /note="from pLacZi; CYC1"
                     /regulatory_class="promoter"
     misc_feature    185..244
                     /gene="gLUC59"
                     /note="from Candida albicans Pga59/orf19.2767 signal
                     peptide and first two amino acid residues of mature domain;
                     PGA59"
     CDS             251..802
                     /label=hGLuc
                     /note="secreted Gaussia luciferase"
     misc_feature    821..1111
                     /gene="gLUC59"
                     /note="from Candida albicans Pga59/orf19.2767 mature domain
                     and GPI-anchor addition domain; PGA59"
     regulatory      1112..1601
                     /note="from CIp10::ACT1p-gLUC59; PGA59"
                     /regulatory_class="terminator"
     primer_bind     complement(1856..1872)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1880..1896)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1904..1934)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1949..1970)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2258..2846)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3020..3877)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3878..3982)
                     /label=AmpR promoter
     CDS             complement(4090..4890)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(4891..5106)
                     /label=URA3 promoter
ORIGIN
        1 aagcttgaat tcgagctcgg tacccgggga tctgtcgacc tcgaggcatg tgctctgtat
       61 gtatataaaa ctcttgtttt cttcttttct ctaaatattc tttccttata cattaggtcc
      121 tttgtagcat aaattactat acttctatag acacgcaaac acaaatacac acactaaatt
      181 aataatgcaa ttctcatccg ctattatctt atctgctgtt gctggttccg ctttagccac
      241 ttacggatcc ggtgttaaag ttttgttcgc tttgatttgt attgctgttg ctgaagctaa
      301 accaaccgaa aacaacgaag atttcaacat tgttgctgtt gcttctaact tcgctaccac
      361 cgatttggat gctgatcgag gtaaattgcc aggtaaaaaa ttgccattgg aagttttgaa
      421 agaaatggaa gctaacgcta ggaaagctgg ttgtaccagg ggttgtttga tttgtttgtc
      481 tcacattaaa tgtaccccaa aaatgaaaaa attcattcca ggtagatgtc acacctacga
      541 aggtgataaa gaatctgctc aaggtggtat tggtgaagct attgttgata ttccagaaat
      601 tccaggtttc aaagatttgg aaccaatgga acaattcatt gctcaagttg atttgtgtgt
      661 tgattgtacc accggttgtt tgaaaggttt ggctaacgtt caatgttctg atttgttgaa
      721 aaaatggttg ccacaaagat gtgctacctt cgcttctaaa attcaaggtc aagttgataa
      781 aaataaaggt gctggtggtg acacccatgg tatggatgaa ttgtacaaag ctaactccac
      841 tgtcactgac attgctacca ccgttgttac catcacttct tgtgaagaaa acaaatgtct
      901 cgaaactgaa gtcactactg gtgtcaccac tgtcactgaa gttgaaacca cttacaccac
      961 ctactgccca ttgccaaccg ccaaagctcc agttgcttct acttctaact ccaccaccac
     1021 cccaccagtt tctactgctg aaggtgctgc tgccgctaac gccgtcccag ccgttgctgc
     1081 cggtttgttg gctttgggtg ctttcatgta agagagttgt cctcaacttt ttttcaattg
     1141 tttaatcaac taaagatttg gggttttgtt ttatttcatt tcttcagatt aatatttttt
     1201 tttaacaaca agaacttaca tttttaataa attataattt atctatcaaa caatcacaaa
     1261 aaaaattttc aaaaaaaaac acttgtagtg tataaattct atatatcttt tggttttatt
     1321 ttgttatgtt tccaattccc attttgtata ttcaaggcta ctgaattata taaagggtat
     1381 atatacatat acaaagaatt gactttcaaa taaaataaat atatattttt atattactac
     1441 tttttttaac ttgagtttat ttcttttcca gagagttgta taatcaattc attaagattc
     1501 tctatatctt tgattgttgt acctttaaca gtgttttgta cctatatgac tcttcgtaat
     1561 tactctatat tctgttcctt atacatcttt gtttctcctg aggaaatcca ttatgtacta
     1621 tttaaaaaac acaaactttt ggatgttcgg tttattcttt ttcttttact tttttatcat
     1681 gggagcctac ttcccgtttt tcccgatttg gctacatgac atcaaccata tcagcaaaag
     1741 tgatacgggt attatttttg ccgctatttc tctgttctcg ctattattcc aaccgctgtt
     1801 tggtctgctt tctgacaaac tcggcctcga ctctagcgaa ttaattcgta atcatgtcat
     1861 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     1921 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     1981 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     2041 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     2101 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     2161 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     2221 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     2281 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     2341 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     2401 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     2461 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     2521 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     2581 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     2641 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     2701 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     2761 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     2821 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     2881 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     2941 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     3001 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     3061 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     3121 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     3181 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     3241 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     3301 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     3361 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     3421 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     3481 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     3541 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     3601 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     3661 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     3721 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     3781 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     3841 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     3901 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     3961 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctggg taataactcg
     4021 atataattaa attgaagctc taatttgtga gtttagtata catgcattta cttataatac
     4081 agttttttag ttttgctggc cgcatcttct caaatatgct tcccagcctg cttttctgta
     4141 acgttcaccc tctaccttag catcccttcc ctttgcaaat agtcctcttc caacaataat
     4201 aatgtcagat cctgtagaga ccacatcatc cacggttcta tactgttgac ccaatgcgtc
     4261 tcccttgtca tctaaaccca caccgggtgt cataatcaac caatcgtaac cttcatctct
     4321 tccacccatg tctctttgag caataaagcc gataacaaaa tctttgtcgc tcttcgcaat
     4381 gtcaacagta cccttagtat attctccagt agatagggag cccttgcatg acaattctgc
     4441 taacatcaaa aggcctctag gttcctttgt tacttcttct gccgcctgct tcaaaccgct
     4501 aacaatacct gggcccacca caccgtgtgc attcgtaatg tctgcccatt ctgctattct
     4561 gtatacaccc gcagagtact gcaatttgac tgtattacca atgtcagcaa attttctgtc
     4621 ttcgaagagt aaaaaattgt acttggcgga taatgccttt agcggcttaa ctgtgccctc
     4681 catggaaaaa tcagtcaaga tatccacatg tgtttttagt aaacaaattt tgggacctaa
     4741 tgcttcaact aactccagta attccttggt ggtacgaaca tccaatgaag cacacaagtt
     4801 tgtttgcttt tcgtgcatga tattaaatag cttggcagca acaggactag gatgagtagc
     4861 agcacgttcc ttatatgtag ctttcgacat gatttatctt cgtttcctgc aggtttttgt
     4921 tctgtgcagt tgggttaaga atactgggca atttcatgtt tcttcaacac tacatatgcg
     4981 tatatatacc aatctaagtc tgtgctcctt ccttcgttct tccttctgtt cggagattac
     5041 cgaatcaaaa aaatttcaag gaaaccgaaa tcaaaaaaaa gaataaaaaa aaaatgatga
     5101 attgaa
//
