LOCUS       40924_47438        6819 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pY1-gLUC59(GW), complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6819)
  AUTHORS   Bonaldi K, Li Z, Kang SE, Breton G, Pruneda-Paz JL.
  TITLE     Novel surface luciferase reporter for high-throughput quantitative 
            test one-hybrid screens
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6819)
  AUTHORS   Pruneda-Paz JL.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2017) Biological Sciences, University of 
            California San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA
REFERENCE   3  (bases 1 to 6819)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6819)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-FEB-2017) Biological Sciences, University of California San 
            Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6819
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    30..154
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        179..209
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             263..919
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             1264..1566
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(1610..1734)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      1759..1897
                     /note="from pLacZi; CYC1"
                     /regulatory_class="promoter"
     misc_feature    1898..1958
                     /gene="gLUC59"
                     /note="from Candida albicans Pga59/orf19.2767 signal
                     peptide and first two amino acid residues of mature domain;
                     PGA59"
     CDS             1964..2515
                     /label=hGLuc
                     /note="secreted Gaussia luciferase"
     misc_feature    2534..2824
                     /gene="gLUC59"
                     /note="from Candida albicans Pga59/orf19.2767 mature domain
                     and GPI-anchor addition domain; PGA59"
     regulatory      2825..3314
                     /note="from CIp10::ACT1p-gLUC59; PGA59"
                     /regulatory_class="terminator"
     primer_bind     complement(3569..3585)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3593..3609)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3617..3647)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3662..3683)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3971..4559)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4733..5590)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5591..5695)
                     /label=AmpR promoter
     CDS             complement(5803..6603)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(6604..6819)
                     /label=URA3 promoter
ORIGIN
        1 aagcttgaat tcgagctcgg tacccatcaa caagtttgta caaaaaagct gaacgagaaa
       61 cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat
      121 aatactgtaa aacacaacat atccagtcac tatggcggcc gcattaggca ccccaggctt
      181 tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc cgtcgagatt
      241 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
      301 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
      361 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
      421 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
      481 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
      541 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
      601 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
      661 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
      721 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
      781 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
      841 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
      901 tgagtggcag ggcggggcgt aaagatctgg atccggctta ctaaaagcca gataacagta
      961 tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag
     1021 tatgtcaaaa agaggtatgc tatgaagcag cgtattacag tgacagttga cagcgacagc
     1081 tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc
     1141 agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg
     1201 ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac aggggctggt
     1261 gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat
     1321 gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca
     1381 cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa
     1441 agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa
     1501 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg
     1561 ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg
     1621 gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat
     1681 attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggttgatgg
     1741 ggatctgtcg acctcgaggc atgtgctctg tatgtatata aaactcttgt tttcttcttt
     1801 tctctaaata ttctttcctt atacattagg tcctttgtag cataaattac tatacttcta
     1861 tagacacgca aacacaaata cacacactaa attaataatg caattctcat ccgctattat
     1921 cttatctgct gttgctggtt ccgctttagc cacttacgga tccggtgtta aagttttgtt
     1981 cgctttgatt tgtattgctg ttgctgaagc taaaccaacc gaaaacaacg aagatttcaa
     2041 cattgttgct gttgcttcta acttcgctac caccgatttg gatgctgatc gaggtaaatt
     2101 gccaggtaaa aaattgccat tggaagtttt gaaagaaatg gaagctaacg ctaggaaagc
     2161 tggttgtacc aggggttgtt tgatttgttt gtctcacatt aaatgtaccc caaaaatgaa
     2221 aaaattcatt ccaggtagat gtcacaccta cgaaggtgat aaagaatctg ctcaaggtgg
     2281 tattggtgaa gctattgttg atattccaga aattccaggt ttcaaagatt tggaaccaat
     2341 ggaacaattc attgctcaag ttgatttgtg tgttgattgt accaccggtt gtttgaaagg
     2401 tttggctaac gttcaatgtt ctgatttgtt gaaaaaatgg ttgccacaaa gatgtgctac
     2461 cttcgcttct aaaattcaag gtcaagttga taaaaataaa ggtgctggtg gtgacaccca
     2521 tggtatggat gaattgtaca aagctaactc cactgtcact gacattgcta ccaccgttgt
     2581 taccatcact tcttgtgaag aaaacaaatg tctcgaaact gaagtcacta ctggtgtcac
     2641 cactgtcact gaagttgaaa ccacttacac cacctactgc ccattgccaa ccgccaaagc
     2701 tccagttgct tctacttcta actccaccac caccccacca gtttctactg ctgaaggtgc
     2761 tgctgccgct aacgccgtcc cagccgttgc tgccggtttg ttggctttgg gtgctttcat
     2821 gtaagagagt tgtcctcaac tttttttcaa ttgtttaatc aactaaagat ttggggtttt
     2881 gttttatttc atttcttcag attaatattt ttttttaaca acaagaactt acatttttaa
     2941 taaattataa tttatctatc aaacaatcac aaaaaaaatt ttcaaaaaaa aacacttgta
     3001 gtgtataaat tctatatatc ttttggtttt attttgttat gtttccaatt cccattttgt
     3061 atattcaagg ctactgaatt atataaaggg tatatataca tatacaaaga attgactttc
     3121 aaataaaata aatatatatt tttatattac tacttttttt aacttgagtt tatttctttt
     3181 ccagagagtt gtataatcaa ttcattaaga ttctctatat ctttgattgt tgtaccttta
     3241 acagtgtttt gtacctatat gactcttcgt aattactcta tattctgttc cttatacatc
     3301 tttgtttctc ctgaggaaat ccattatgta ctatttaaaa aacacaaact tttggatgtt
     3361 cggtttattc tttttctttt acttttttat catgggagcc tacttcccgt ttttcccgat
     3421 ttggctacat gacatcaacc atatcagcaa aagtgatacg ggtattattt ttgccgctat
     3481 ttctctgttc tcgctattat tccaaccgct gtttggtctg ctttctgaca aactcggcct
     3541 cgactctagc gaattaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc
     3601 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     3661 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     3721 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     3781 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     3841 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     3901 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     3961 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     4021 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     4081 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     4141 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     4201 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     4261 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     4321 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     4381 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     4441 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     4501 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     4561 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     4621 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     4681 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     4741 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     4801 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     4861 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     4921 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     4981 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     5041 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     5101 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     5161 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     5221 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     5281 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     5341 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     5401 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     5461 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     5521 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     5581 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     5641 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     5701 ttccccgaaa agtgccacct gggtaataac tcgatataat taaattgaag ctctaatttg
     5761 tgagtttagt atacatgcat ttacttataa tacagttttt tagttttgct ggccgcatct
     5821 tctcaaatat gcttcccagc ctgcttttct gtaacgttca ccctctacct tagcatccct
     5881 tccctttgca aatagtcctc ttccaacaat aataatgtca gatcctgtag agaccacatc
     5941 atccacggtt ctatactgtt gacccaatgc gtctcccttg tcatctaaac ccacaccggg
     6001 tgtcataatc aaccaatcgt aaccttcatc tcttccaccc atgtctcttt gagcaataaa
     6061 gccgataaca aaatctttgt cgctcttcgc aatgtcaaca gtacccttag tatattctcc
     6121 agtagatagg gagcccttgc atgacaattc tgctaacatc aaaaggcctc taggttcctt
     6181 tgttacttct tctgccgcct gcttcaaacc gctaacaata cctgggccca ccacaccgtg
     6241 tgcattcgta atgtctgccc attctgctat tctgtataca cccgcagagt actgcaattt
     6301 gactgtatta ccaatgtcag caaattttct gtcttcgaag agtaaaaaat tgtacttggc
     6361 ggataatgcc tttagcggct taactgtgcc ctccatggaa aaatcagtca agatatccac
     6421 atgtgttttt agtaaacaaa ttttgggacc taatgcttca actaactcca gtaattcctt
     6481 ggtggtacga acatccaatg aagcacacaa gtttgtttgc ttttcgtgca tgatattaaa
     6541 tagcttggca gcaacaggac taggatgagt agcagcacgt tccttatatg tagctttcga
     6601 catgatttat cttcgtttcc tgcaggtttt tgttctgtgc agttgggtta agaatactgg
     6661 gcaatttcat gtttcttcaa cactacatat gcgtatatat accaatctaa gtctgtgctc
     6721 cttccttcgt tcttccttct gttcggagat taccgaatca aaaaaatttc aaggaaaccg
     6781 aaatcaaaaa aaagaataaa aaaaaaatga tgaattgaa
//
