LOCUS       40924_47348        6416 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pXtale, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6416)
  AUTHORS   Pesce C, Koebnik R.
  TITLE     TALE expression vector for clade-1 xanthomonads
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6416)
  AUTHORS   Pesce C, Koebnik R.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2017) UMR 186 IPME, IRD Montpellier, 911, Avenue 
            Agropolis, Montpellier, Languedoc-Roussillon 34394, France
REFERENCE   3  (bases 1 to 6416)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6416)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-JAN-2017) UMR 186 IPME, IRD Montpellier, 911, Avenue Agropolis, 
            Montpellier, Languedoc-Roussillon 34394, France"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6416
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1023..1792
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             1793..2452
                     /codon_start=1
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     primer_bind     3162..3178
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3188..3206
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(3245..4912)
                     /codon_start=1
                     /gene="TAL"
                     /product="TAL effector repeat array acceptor"
                     /label=TAL
                     /note="TAL effector derivative without repeat array"
                     /protein_id="ASM90837.1"
                     /translation="MEPIRSRTPSTARELQAGSQPDAVQPIADRLVSTPASSPLDGLPA
                     RRMVSRTSPPPSPARSPAFSAGSLSGLLRQQIDPSLFAGSPFDSLPSFGAARAESAPGE
                     GDEVQSALRAVDDPQPSASAAITAAPPRTKAAARRRSAQTSDALPAAHVDLGTFGYSQQ
                     QQEKIKPKVRSTVAQHHEALVGHGFTHAHIVELSKHPPALGTIAARYSEMIAALPEATH
                     EDIVGVGKQKSGARALEALLTVAEELRAPPLQLVTGQLLKIAKRGGVNAVEVRPASSST
                     RVSPDPALAALTNDRLVALACLGGRPALDAVKKGLPHAPALVTRTHNRVPEGTAHLVAD
                     HAQVVRVLGFFQCHSHPAQAFHEAMTRFEMSREGLLQLFRRVGVTELEAISGTLPPASQ
                     RWHRILQALGVKGAKPSSASAQTPSQESVDAFADSLERELDAPSPIHEADRARASNRKR
                     SRSESSVNRSSAQQAAEVFVPEQRDAPPLLPLSSWGVKRQRTRIGGLPDPGMPTDGELA
                     ASSAAFLEQDADPFAGAAEDFPVFDQEEIAWLMTLLPH"
     primer_bind     complement(4937..4953)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(4983..5001)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5022..5038)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5046..5062)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5070..5100)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5115..5136)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5406..5434
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             5623..6153
                     /codon_start=1
                     /label=GmR
                     /note="gentamycin acetyltransferase"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 ctcgggccgt ctcttgggct tgatcggcct tcttgcgcat ctcacgcgct cctgcggcgg
       61 cctgtagggc aggctcatac ccctgccgaa ccgcttttgt cagccggtcg gccacggctt
      121 ccggcgtctc aacgcgcttt gagattccca gcttttcggc caatccctgc ggtgcatagg
      181 cgcgtggctc gaccgcttgc gggctgatgg tgacgtggcc cactggtggc cgctccaggg
      241 cctcgtagaa cgcctgaatg cgcgtgtgac gtgccttgct gccctcgatg ccccgttgca
      301 gccctagatc ggccacagcg gccgcaaacg tggtctggtc gcgggtcatc tgcgctttgt
      361 tgccgatgaa ctccttggcc gacagcctgc cgtcctgcgt cagcggcacc acgaacgcgg
      421 tcatgtgcgg gctggtttcg tcacggtgga tgctggccgt cacgatgcga tccgccccgt
      481 acttgtccgc cagccacttg tgcgccttct cgaagaacgc cgcctgctgt tcttggctgg
      541 ccgacttcca ccattccggg ctggccgtca tgacgtactc gaccgccaac acagcgtcct
      601 tgcgccgctt ctctggcagc aactcgcgca gtcggcccat cgcttcatcg gtgctgctgg
      661 ccgcccagtg ctcgttctct ggcgtcctgc tggcgtcagc gttgggcgtc tcgcgctcgc
      721 ggtaggcgtg cttgagactg gccgccacgt tgcccatttt cgccagcttc ttgcatcgca
      781 tgatcgcgta tgccgccatg cctgcccctc ccttttggtg tccaaccggc tcgacggggg
      841 cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg agtatactca ctagactttg
      901 cttcgcaaag tcgtgaccgc ctacggcggc tgcggcgccc tacgggcttg ctctccgggc
      961 ttcgccctgc gcggtcgctg cgctcccttg ccagcccgtg gatatgtgga cgatggccgc
     1021 gagcggccac cggctggctc gcttcgctcg gcccgtggac aaccctgctg gacaagctga
     1081 tggacaggct gcgcctgccc acgagcttga ccacagggat tgcccaccgg ctacccagcc
     1141 ttcgaccaca tacccaccgg ctccaactgc gcggcctgcg gccttgcccc atcaattttt
     1201 ttaattttct ctggggaaaa gcctccggcc tgcggcctgc gcgcttcgct tgccggttgg
     1261 acaccaagtg gaaggcgggt caaggctcgc gcagcgaccg cgcagcggct tggccttgac
     1321 gcgcctggaa cgacccaagc ctatgcgagt gggggcagtc gaaggcgaag cccgcccgcc
     1381 tgccccccga gcctcacggc ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg
     1441 caaggccgaa ggccgcgcag tcgatcaaca agccccggag gggccacttt ttgccggagg
     1501 gggagccgcg ccgaaggcgt gggggaaccc cgcaggggtg cccttctttg ggcaccaaag
     1561 aactagatat agggcgaaat gcgaaagact taaaaatcaa caacttaaaa aaggggggta
     1621 cgcaacagct cattgcggca ccccccgcaa tagctcattg cgtaggttaa agaaaatctg
     1681 taattgactg ccacttttac gcaacgcata attgttgtcg cgctgccgaa aagttgcagc
     1741 tgattgcgca tggtgccgca accgtgcggc accctaccgc atggagataa gcatggccac
     1801 gcagtccaga gaaatcggca ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga
     1861 acgcaaagcg catgaggcgt gggccgggct tattgcgagg aaacccacgg cggcaatgct
     1921 gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca gccagaagac
     1981 actttccaag ctcatcggac gttctttgcg gacggtccaa tacgcagtca aggacttggt
     2041 ggccgagcgc tggatctccg tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt
     2101 ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag
     2161 tgccgccgtg gtggttgatc acgacgacca ggacgaatcg ctgttggggc atggcgacct
     2221 gcgccgcatc ccgaccctgt atccgggcga gcagcaacta ccgaccggcc ccggcgagga
     2281 gccgcccagc cagcccggca ttccgggcat ggaaccagac ctgccagcct tgaccgaaac
     2341 ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc cgtgttttct
     2401 ggacgatggc gagccgttgg agccgccgac acgggtcacg ctgccgcgcc ggtagcactt
     2461 gggttgcgca gcaacccgta agtgcgctgt tccagactat cggctgtagc cgcctcgccg
     2521 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga
     2581 cctgaatgga agccggcggc acctcgctaa cggattcacc gtttttatca ggctctggga
     2641 ggcagaataa atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg
     2701 gcctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa
     2761 ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa
     2821 tttgctttcg aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaccag
     2881 gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc
     2941 agtcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     3001 aaatattaac gcttacaatt tccattcgcc attcaggctg cgcaactgtt gggaagggcg
     3061 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     3121 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     3181 gcgcgcgtaa tacgactcac tatagggcga attggagctc caccgcggtg gcggccgctc
     3241 tagatcaatg aggcaatagc gtcatcaacc atgcgatctc ctcttggtcg aataccggga
     3301 aatcttccgc tgcgcctgcg aagggatccg catcttgctc caagaacgca gcgctggacg
     3361 ctgccagttc gccgtccgtc ggcatgccag gatccgggag gccgccgatc ctggtacgct
     3421 ggcgttttac gccccagcta ctgaggggga gcaaaggcgg tgcatcgcgc tgttcgggaa
     3481 cgaatacctc ggcagcctgc tgtgcagagg agcggttgac agaactctct gatcgggacc
     3541 gtttgcggtt gcttgcccga gcccggtctg cctcgtgaat tgggctgggc gcgtccagtt
     3601 cacgctccag ggaatcggcg aatgcatcca cggactcctg gctcggagtt tgagccgaag
     3661 cagaggacgg cttcgcccct ttcaccccta atgcttgaag gatgcggtgc caacgctggg
     3721 aggctggggg aagcgttcca ctgatggctt cgagttctgt gacgcccaca cggcgaaata
     3781 gctgcaacaa tccttccctg ctcatctcaa accgcgtcat ggcttcatga aatgcttgcg
     3841 ctgggtggga gtggcactgg aaaaacccca gcacgcgaac cacttgcgcg tggtcggcaa
     3901 ccagatgggc cgtgccttcg ggaacacggt tatgggttct tgtgaccaat gccggcgcgt
     3961 gcggcaatcc ctttttcacc gcatccagcg caggacgtcc gccgaggcag gccaaggcga
     4021 cgaggcggtc gttggtcaac gcggccaagg ccggatcagg tgagaccctc gttgatgagc
     4081 tcgcaggtct cacctccacc gcgttcacgc cgccacgttt tgcaatcttg aggagttggc
     4141 ctgtgaccaa ctgtaacggt ggagctctca actcttccgc caccgtgagc aaggcctcca
     4201 gggcccgtgc gcccgacttc tgtttgccga cgccgacgat gtcttcgtgt gtcgcctctg
     4261 gcaacgccgc aatcatctcc gaatacctgg ccgctatggt ccctagcgct ggcgggtgtt
     4321 tgctgagctc aacgatgtgc gcgtgtgtaa acccatggcc gaccagtgcc tcgtggtgct
     4381 gcgccactgt cgaacgcacc ttcggtttga tcttctcctg ttgctgctgg ctgtagccga
     4441 acgtgcctag atccacatgc gcggcaggca aagcgtcgga ggtctgggca gaacgccgcc
     4501 gcgccgccgc tttggtgcgc ggcggcgcgg cagtgatcgc cgcacttgct gagggctgcg
     4561 ggtcatctac tgcacgcaga gccgattgca cctcatcccc ctctcctgga gcagactctg
     4621 cacgagcagc gccgaaggaa ggcaatgaat caaaaggcga tccagcaaaa agcgacggat
     4681 caatctgctg acgtagcagg ccgctcaagc tgcccgctga aaacgccggc gagcgtgcag
     4741 gggagggcgg cggactggtc cgggatacca tccgccgagc gggcaagcca tccaggggac
     4801 tgctggcagg cgtagacacc aaccgatccg cgatgggctg gaccgcatcc ggctgagatc
     4861 cggcctgaag ctcgcgagca gtacttggcg tgcgggagcg aataggctcc atcagtcatt
     4921 cctctttcaa gcttatcgat accgtcgacc tcgagggggg gcccggtacc cagcttttgt
     4981 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
     5041 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     5101 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     5161 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     5221 ggcggtttgc gtattgggcg catgcataaa aactgttgta attcattaag cattctgccg
     5281 acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg
     5341 tcgccttgcg tataatattt gcccatggac gcacaccgtg gaaacggatg aaggcacgaa
     5401 cccagttgac ataagcctgt tcggttcgta aactgtaatg caagtagcgt atgcgctcac
     5461 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
     5521 tggcttgtta tgactgtttt tttgtacagt ctatgcctcg ggcatccaag cagcaagcgc
     5581 gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagcaac
     5641 gatgttacgc agcagggcag tcgccctaaa acaaagttag gtggctcaag tatgggcatc
     5701 attcgcacat gtaggctcgg ccctgaccaa gtcaaatcca tgcgggctgc tcttgatctt
     5761 ttcggtcgtg agttcggaga cgtagccacc tactcccaac atcagccgga ctccgattac
     5821 ctcgggaact tgctccgtag taagacattc atcgcgcttg ctgccttcga ccaagaagcg
     5881 gttgttggcg ctctcgcggc ttacgttctg cccaggtttg agcagccgcg tagtgagatc
     5941 tatatctatg atctcgcagt ctccggcgag caccggaggc agggcattgc caccgcgctc
     6001 atcaatctcc tcaagcatga ggccaacgcg cttggtgctt atgtgatcta cgtgcaagca
     6061 gattacggtg acgatcccgc agtggctctc tatacaaagt tgggcatacg ggaagaagtg
     6121 atgcactttg atatcgaccc aagtaccgcc acctaacaat tcgttcaagc cgagatcggc
     6181 ttcccggccg cggagttgtt cggtaaattg tcacaacgcc gccaggtggc acttttcggg
     6241 gaaatgtgcg cgcccgcgtt cctgctggcg ctgggcctgt ttctggcgct ggacttcccg
     6301 ctgttccgtc agcagctttt cgcccacggc cttgatgatc gcggcggcct tggcctgcat
     6361 atcccgattc aacggcccca gggcgtccag aacgggcttc aggcgctccc gaagat
//
