LOCUS       V002027                 6182 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002027
VERSION     V002027
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6182)
  AUTHORS   Moe SE, Sorbo JG, Sogaard R, Zeuthen T, Petter Ottersen O, Holen T.
  TITLE     New isoforms of rat Aquaporin-4
  JOURNAL   Genomics 91 (4), 367-377 (2008)
   PUBMED   18255256
REFERENCE   2  (bases 1 to 6182)
  AUTHORS   Sorbo JG, Moe SE, Holen TV.
  TITLE     New Isoforms of Rat Aquaporin-4
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 6182)
  AUTHORS   Sorbo JG, Moe SE, Holen TV.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-FEB-2007) CMBN, University of Oslo, Sognsvannsveien 9,
            Oslo, no 0317, Norway
REFERENCE   4  (bases 1 to 6182)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 6182)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genomics";
            date: "2008"; volume: "91"; issue: "4"; pages: "367-377"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (12-FEB-2007) CMBN, University of Oslo, Sognsvannsveien 9, Oslo, no
            0317, Norway"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6182
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        224..603
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        604..807
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        852..870
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           903..945
                     /label="Xenopus globin 5'-UTR"
                     /note="translational enhancer from the 5'-UTR of the major
                     beta-globin gene of Xenopus laevis"
     CDS             1102..2070
                     /gene="Aqp4"
                     /label="Aquaporin-4"
                     /note="Aquaporin-4 from Rattus norvegicus. Accession#:
                     P47863"
     3'UTR           2153..2278
                     /label="Xenopus globin 3'-UTR"
                     /note="3'-UTR of the major beta-globin gene of Xenopus
                     laevis"
     polyA_signal    2424..2648
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2694..3122
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3136..3466
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        3514..3561
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             3580..4284
                     /label="EGFP"
                     /note="enhanced GFP"
     CDS             4291..5079
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5256..5389
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(5519..6107)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 agatctcccg atcccctatg gtcgactctc agtacaatct gctctgatgc cgcatagtta
       61 agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt
      121 taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg
      181 cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact
      241 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
      301 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      361 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      421 tgggtggact atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      481 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      541 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      601 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      661 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      721 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      781 cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac tgcttactgg
      841 cttatcgaaa ttaatacgac tcactatagg gagacccaag ctggctagag gtacccagct
      901 tgcttgttct ttttgcagaa gctcagaata aacgctcaac tttggcagat caattccccg
      961 gggatccgaa ttcccaccat ggtgcagaat ctttccaccc ctacactcca aaaacccaat
     1021 cagacaagtg cccgtaatct gattcccgag cactggagcc tgggggcagg caatgagagc
     1081 tgcactccgg ccagggaagg catgagtgac ggagctgcag cgaggcggtg gggtaagtgt
     1141 ggacctccct gcagcagaga gagcatcatg gtggctttca aaggcgtctg gactcaagcc
     1201 ttctggaagg cggtcacagc agagttcctg gccatgctca tctttgttct gctcagcgtg
     1261 ggatccacca ttaactgggg tggctcagag aaccccctac ctgtggacat ggtcctcatc
     1321 tccctctgct ttggactcag cattgccacc atggttcagt gcttcggcca catcagcggt
     1381 ggccacatca acccagcggt gacagtggcc atggtgtgca cacgaaagat cagcatcgcc
     1441 aagtccgtct tctacatcac tgcgcagtgc ctgggggcca tcatcggagc tgggatcctc
     1501 tacctggtca caccccccag cgtggtggga ggattgggag tcaccacggt tcatggaaac
     1561 ctcactgctg gccatgggct cctggtggag ctaataatca ctttccagct ggtattcacc
     1621 atttttgcca gctgtgattc caaacggact gatgttactg gttccgttgc tttagcaatt
     1681 gggttttccg ttgcaattgg acatttgttt gcaatcaatt ataccggagc cagcatgaat
     1741 ccagctcgat cctttggccc tgcagttatc atgggaaact gggaaaacca ctggatatat
     1801 tgggttggac caatcatagg cgctgtgctg gcaggtgcac tttacgagta tgtcttctgt
     1861 cctgacgtgg agctcaaacg tcgcctaaag gaagccttca gcaaagctgc acagcagacg
     1921 aaagggagct acatggaggt ggaggacaac cggagccaag tggagacaga agacttgatc
     1981 ctgaagcccg gggtggtgca tgtgatcgac attgaccgtg gagacgagaa gaaggggaag
     2041 gactcgtctg gagaggtatt atcttctgta tgactagagg acagcactga aaacagaaga
     2101 gactccctag aactggcggc cgcctagaaa tagcttgatc tggttaccac taaaccagcc
     2161 tcaagaacac ccgaatggag tctctaagct acataatacc aacttacact ttacaaaatg
     2221 ttgtccccca aaatgtagcc attcgtatct gctcctaata aaaagaaagt ttcttcacat
     2281 tctaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaccc cccccccccc ccccctgcag
     2341 agatctgcta gcttgagtat tctatagagt cacctaaata ctcgagtcta gagggcccgt
     2401 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     2461 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     2521 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     2581 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     2641 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     2701 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     2761 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     2821 cggctttccc cgtcaagctc taaatcgggg catcccttta gggttccgat ttagtgcttt
     2881 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     2941 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     3001 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     3061 tttggggatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     3121 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccaggcagg
     3181 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     3241 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     3301 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     3361 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     3421 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     3481 ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat
     3541 atcggcatag tataatacga caaggtgagg aactaaacca tggtgagcaa gggcgaggag
     3601 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     3661 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     3721 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     3781 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     3841 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     3901 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     3961 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     4021 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     4081 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     4141 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     4201 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     4261 gccgggatca ctctcggcat ggacggtacc attgaacaag atggattgca cgcaggttct
     4321 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
     4381 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
     4441 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc
     4501 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
     4561 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
     4621 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
     4681 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
     4741 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
     4801 gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
     4861 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     4921 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     4981 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     5041 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg
     5101 aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct
     5161 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     5221 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
     5281 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     5341 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgtccg
     5401 gacactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     5461 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     5521 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     5581 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     5641 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     5701 tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     5761 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     5821 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     5881 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     5941 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     6001 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     6061 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     6121 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     6181 tg
//
