LOCUS       40924_47398        7014 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pXZ240, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 5 to 48)
  AUTHORS   Zeng X, Herndon AM, Hu JC.
  TITLE     Buried asparagines determine the dimerization specificities of 
            leucine zipper mutants
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 94 (8), 3673-3678 (1997)
  PUBMED    9108036
REFERENCE   2  (bases 1 to 7014)
  AUTHORS   Marino-Ramirez L, Hu JC.
  TITLE     Using lambda repressor fusions to isolate and characterize 
            self-assembling domains
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 7014)
  AUTHORS   Marino-Ramirez L, Hu JC.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   4  (bases 1 to 7014)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 7014)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "1997"; volume: "94"; issue: "8"; pages: 
            "3673-3678"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (25-OCT-2000) Biochemistry "
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7014
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      5..48
                     /note="HH7107; weak constitutive promoter derived from
                     lacUV5"
                     /citation=[1]
                     /regulatory_class="promoter"
     CDS             73..501
                     /codon_start=1
                     /product="cI binding domain"
                     /label=cI binding domain
                     /note="cI-DBD"
                     /protein_id="AAK07890.1"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPKLRTFTKGDAERWVSTDRSRRFTTS"
     CDS             2408..2473
                     /label=GCN4_v1
                     /note="GCN4 peptide that allows binding to an scFv-GFP
                     fusion protein (Tanenbaum et al., 2014)"
     terminator      2543..2590
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             4075..4263
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    4368..4508
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4694..5282)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      5393..5906
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(5951..6808)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6809..6913)
                     /label=AmpR promoter
ORIGIN
        1 aattctaggc tttatgcttt atgcttccgg ctcgtacatt gtgtggaggt acctctagac
       61 aggaaacagc gtatgagcac aaaaaagaaa ccattaacac aagagcagct tgaggacgca
      121 cgtcgcctta aagcaattta tgaaaaaaag aaaaatgaac ttggcttatc ccaggaatct
      181 gtcgcagaca agatggggat ggggcagtca ggcgttggtg ctttatttaa tggcatcaat
      241 gcattaaatg cttataacgc cgcattgctt gcaaaaattc tcaaagttag cgttgaagaa
      301 tttagccctt caatcgccag agaaatctac gagatgtatg aagcggttag tatgcagccg
      361 tcacttagaa gtgagtatga gtaccctgtt ttttctcatg ttcaggcagg gatgttctca
      421 cctaagctta gaacctttac caaaggtgat gcggagagat gggtgtcgac ggatcgatcc
      481 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
      541 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
      601 ccaacagttg cgcagcctga atggcgaatg gcgctttgcc tggtttccgg caccagaagc
      661 ggtgccggaa agctggctgg agtgcgatct tcctgaggcc gatactgtcg tcgtcccctc
      721 aaactggcag atgcacggtt acgatgcgcc catctacacc aacgtaacct atcccattac
      781 ggtcaatccg ccgtttgttc ccacggagaa tccgacgggt tgttactcgc tcacatttaa
      841 tgttgatgaa agctggctac aggaaggcca gacgcgaatt atttttgatg gcgttaactc
      901 ggcgtttcat ctgtggtgca acgggcgctg ggtcggttac ggccaggaca gtcgtttgcc
      961 gtctgaattt gacctgagcg catttttacg cgccggagaa aaccgcctcg cggtgatggt
     1021 gctgcgttgg agtgacggca gttatctgga agatcaggat atgtggcgga tgagcggcat
     1081 tttccgtgac gtctcgttgc tgcataaacc gactacacaa atcagcgatt tccatgttgc
     1141 cactcgcttt aatgatgatt tcagccgcgc tgtactggag gctgaagttc agatgtgcgg
     1201 cgagttgcgt gactacctac gggtaacagt ttctttatgg cagggtgaaa cgcaggtcgc
     1261 cagcggcacc gcgcctttcg gcggtgaaat tatcgatgag cgtggtggtt atgccgatcg
     1321 cgtcacacta cgtctgaacg tcgaaaaccc gaaactgtgg agcgccgaaa tcccgaatct
     1381 ctatcgtgcg gtggttgaac tgcacaccgc cgacggcacg ctgattgaag cagaagcctg
     1441 cgatgtcggt ttccgcgagg tgcggattga aaatggtctg ctgctgctga acggcaagcc
     1501 gttgctgatt cgaggcgtta accgtcacga gcatcatcct ctgcatggtc aggtcatgga
     1561 tgagcagacg atggtgcagg atatcctgct gatgaagcag aacaacttta acgccgtgcg
     1621 ctgttcgcat tatccgaacc atccgctgtg gtacacgctg tgcgaccgct acggcctgta
     1681 tgtggtggat gaagccaata ttgaaaccca cggcatggtg ccaatgaatc gtctgaccga
     1741 tgatccgcgc tggctaccgg cgatgagcga acgcgtaacg cgaatggtgc agcgcgatcg
     1801 taatcacccg agtgtgatca tctggtcgct ggggaatgaa tcaggccacg gcgctaatca
     1861 cgacgcgctg tatcgctgga tcaaatctgt cgatccttcc cgcccggtgc agtatgaagg
     1921 cggcggagcc gacaccacgg ccaccgatat tatttgcccg atgtacgcgc gcgtggatga
     1981 agaccagccc ttcccggctg tgccgaaatg gtccatcaaa aaatggcttt cgctacctgg
     2041 agagacgcgc ccgctgatcc tttgcgaata cgcccacgcg atgggtaaca gtcttggcgg
     2101 tttcgctaaa tactggcagg cgtttcgtca gtatccccgt ttacagggcg gcttcgtctg
     2161 ggactgggtg gatcagtcgc tgattaaata tgatgaaaac ggcaacccgt ggtcggctta
     2221 cggcggtgat tttggcgata cgccgaacga tcgccagttc tgtatgaacg gtctggtctt
     2281 tgccgaccgc acgccgcatc cagcgctgac ggaagcaaaa caccagcagc agtttttcca
     2341 gttccgttta tccgggcaaa ccatcgaagt gaccagcgaa tacctgttcc gtcatagcga
     2401 taacgagctc ctgtctaaaa actaccacct cgagaacgaa gttgcgcgcc tgaaaaaact
     2461 agttggtgaa cgttgaggat ccggcgctgc taacaaagcc cgaaaggaag ctgagttggc
     2521 tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag
     2581 gggttttttg ctgaaaggag gaactatatg cccggatatc gtccattccg acagcatcgc
     2641 cagtcactat ggcgtgctgc tagcgctata tgcgttgatg caatttctat gcgcacccgt
     2701 tctcggagca ctgtccgacc gctttggccg ccgcccagtc ctgctcgctt cgctacttgg
     2761 agccactatc gactacgcga tcatggcgac cacacccgtc ctgtggatct cgaccgatgc
     2821 ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg
     2881 ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct
     2941 gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg
     3001 cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc gccaccaaac
     3061 gtttcggcga gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct
     3121 tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg
     3181 gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc
     3241 agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc actggaccgc
     3301 tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg
     3361 taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg
     3421 ccacctcgac ctgaatggaa gccggcggca cctcgctaac ggattcacca ctccaagaat
     3481 tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt ggcagaacat
     3541 atccatcgcg tccgccatct ccagcagccg cacgcggcgc atctcgggca gcgttgggtc
     3601 ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg
     3661 ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg
     3721 ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta
     3781 aagtctggaa acgcggaagt cagcgccctg caccattatg ttccggatct gcatcgcagg
     3841 atgctgctgg ctaccctgtg gaacacctac atctgtatta acgaagcgct ggcattgacc
     3901 ctgagtgatt tttctctggt cccgccgcat ccataccgcc agttgtttac cctcacaacg
     3961 ttccagtaac cgggcatgtt catcatcagt aacccgtatc gtgagcatcc tctctcgttt
     4021 catcggtatc attaccccca tgaacagaaa ttccccctta cacggaggca tcaagtgacc
     4081 aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt
     4141 ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac
     4201 gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     4261 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     4321 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     4381 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
     4441 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     4501 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     4561 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     4621 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     4681 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     4741 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     4801 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     4861 tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt
     4921 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     4981 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     5041 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     5101 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     5161 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     5221 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     5281 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     5341 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttacgcgccc
     5401 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     5461 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     5521 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     5581 cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc
     5641 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     5701 ttccaaactg gaacaacact caaccctatc tcgggctatt cttttgattt ataagggatt
     5761 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     5821 tttaacaaaa tattaacgtt tacaatttaa atatttgctt atacaatctt cctgtttttg
     5881 gggcttttct gattatcaac cggggtaaat caatctaaag tatatatgag taaacttggt
     5941 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     6001 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     6061 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     6121 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     6181 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     6241 tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg
     6301 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     6361 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     6421 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     6481 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     6541 cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa
     6601 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     6661 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     6721 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     6781 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     6841 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     6901 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     6961 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt caag
//
