LOCUS       V014336                 9763 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014336
VERSION     V014336
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9763)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9763
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1087..1275
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    1380..1520
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1706..2294)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2468..3325)
                     /label="AmpR"
                     /note="beta-lactamase"
     terminator      4074..4108
                     /label="lambda t0 terminator"
                     /note="minimal transcription terminator from phage lambda
                     (Scholtissek and Grosse, 1987)"
     CDS             6460..7515
                     /gene="ERG20"
                     /label="Farnesyl pyrophosphate synthase"
                     /note="Farnesyl pyrophosphate synthase from Saccharomyces
                     cerevisiae (strain ATCC 204508 / S288c). Accession#:
                     P08524"
     CDS             7516..7533
                     /label="6xHis"
                     /note="6xHis affinity tag"
     promoter        complement(7582..7686)
                     /label="AmpR promoter"
     CDS             8065..8799
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     terminator      8972..9058
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      9077..9104
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
ORIGIN
        1 atcctgatat tgtctgcatt tgcgccggag caaaccaaaa acctggtgag acacgccttg
       61 aattagtaga aaagaacttg aagattttca aaggcatcgt tagtgaagtc atggcgagcg
      121 gatttgacgg cattttctta gtcgcgacgc gaggctggat ggccttcccc attatgattc
      181 ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag
      241 atgacgacca tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga
      301 tcactggacc gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt
      361 tggcatggat tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg
      421 catggagccg ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac
      481 cactccaaga attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc
      541 ttggcagaac atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg
      601 cagcgttggg tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct
      661 aggctggcgg ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg
      721 aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt
      781 ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat
      841 ctgcatcgca ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg
      901 ctggcattga ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt
      961 accctcacaa cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat
     1021 cctctctcgt ttcatcggta tcattacccc catgaacaga aattccccct tacacggagg
     1081 catcaagtga ccaaacagga aaaaaccgcc cttaacatgg cccgctttat cagaagccag
     1141 acattaacgc ttctggagaa actcaacgag ctggacgcgg atgaacaggc agacatctgt
     1201 gaatcgcttc acgaccacgc tgatgagctt taccgcagct gcctcgcgcg tttcggtgat
     1261 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     1321 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     1381 gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat
     1441 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     1501 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     1561 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     1621 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     1681 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     1741 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     1801 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     1861 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc
     1921 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     1981 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     2041 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     2101 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     2161 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     2221 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     2281 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     2341 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     2401 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     2461 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     2521 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     2581 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     2641 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     2701 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     2761 gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     2821 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     2881 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     2941 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     3001 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     3061 gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag
     3121 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     3181 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     3241 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     3301 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     3361 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     3421 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     3481 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa gaatgatggg
     3541 ctaatgtgtg tttacgacaa ttctcacttc atacttttcc atcgtcaggt cgcctgacaa
     3601 tatgtctcct gtcattatgt ccttcacact ctgatcaaac gtgaccagct gtttttcttc
     3661 cgtgaaattc atgacaaaaa tataatcatt gtcctgatcc tgcctcgctt gtacggagac
     3721 gccttttccg tgccgaaccg gaaaaactgg agagagagac aggtctgtga tcagaccctc
     3781 atagaaatca cgctgaaatt gatcctccaa acgcgcgccg ataaaatacg ccttgccctg
     3841 ctgatactca tggcttgtga ccgctggcgt gcgcgcataa aaatcttctt gatacaccgc
     3901 ttccactgaa gctgtcttta catcaatcac ggttgcataa tccttcattt catatatttg
     3961 gctgcggtag ctgacagcgt ttcgatcctt cggatacagg gtgtccgttt caagaggctc
     4021 aactccaaat atagcttgaa atcgatatct ctgcagtcgc gatgattaat taattcagaa
     4081 cgctcggttg ccgccgggcg ttttttatgc agcaatggca agaacgtccc ggggagctcc
     4141 taacttatag gggtaacact taaaaaagaa tcaataacga tagaaaccgc tcctaaagca
     4201 ggtgcatttt ttcctaacga agaaggcaat agttcacatt tattgtctaa atgagaatgg
     4261 actctagaag aaacttcgtt tttaatcgta tttaaaacaa tgggatgaga ttcaattata
     4321 tgatttctca agataacagc ttctatatca aatgtattaa ggatattggt taatccaatt
     4381 ccgatataaa agccaaagtt ttgaagtgca tttaacattt ctacatcatt tttatttgcg
     4441 cgttccacaa tctcttttcg agaaatattc ttttcttctt tagagagcga agccagtaac
     4501 gctttttcag aagcatataa ttcccaacag cctcgatttc cacagctgca tttgggtcca
     4561 ttaaaatcta tcgtcatatg acccatttcc ccagaaaaac cctgaacacc tttatacaat
     4621 tcgttgttaa taacaagtcc agttccaatt ccgatattaa tactgatgta aacgatgttt
     4681 tcatagtttt ttgtcatacc aaatactttt tcaccgtatg ctcctgcatt agcttcattt
     4741 tcaacaaaaa ccggaacatt aaactcactc tcaattaaaa actgcaaatc tttgatattc
     4801 caatttaagt taggcatgaa aataatttgc tgatgacgat ctacaaggcc tggaacacaa
     4861 attcctattc cgactagacc ataaggggac tcaggcatat gggttacaaa accatgaata
     4921 agtgcaaata aaatctcttt tacttcacta gcggaagaac tagacaagtc agaagtcttc
     4981 tcgagaataa tatttccttc taagtcggtt agaattccgt taagatagtc gactcctata
     5041 tcaataccaa tcgagtagcc tgcattctta ttaaaaacaa gcattacagg tcttctgccg
     5101 cctctagatt gccctgcccc aatttcaaaa ataaaatctt tttcaagcag tgtatttact
     5161 tgagaggaga cagtagactt gtttaatcct gtaatctcag agagagttgc cctggagaca
     5221 ggggagttct tcaaaatttc atctaatatt aatttttgat tcattttttt tactaaagct
     5281 tgatctgcaa tttgaataat aaccactcct ttgtttatcc accgaactaa gttggtgttt
     5341 tttgaagctt gaattagata tttaaaagta tcatatctaa tattataact aaattttcta
     5401 aaaaaaacat tgaaataaac atttattttg tatatgatga gataaagtta gtttattgga
     5461 taaacaaact aactcaatta agatagttga tggataaact tgttcactta aatcaaaggg
     5521 ggaaatgaca aatggtccaa actagtgata tctaaaaatc aaagggggaa atgggatccg
     5581 gtaccattct gctagcagat gtctgttcct aacaaacttc gtgataacgc tatggttatg
     5641 cttaaagaaa catctcgtac attcttcatc cctatctctc atcttcctgc tgaacttcaa
     5701 aacgctgttg gctctgctta cctttgcatg cgtgctatcg atgaaatcga agatcatcct
     5761 gaacttgaag ctggcgttaa atctcgtctt ctttacgcta tctctgatct tcttaaaaaa
     5821 tctttcaacg aagatgaata catggctctt gttggccctt accaagctga tcttcctgat
     5881 gttacacttc aacttggcaa ctggatcgct ctttgcccta caggcgttgc tgatcaagtt
     5941 cttgatgcta catctatcat ggctaaaggc atggctgatt gggttgaaaa agattggcat
     6001 atccaaaacg aagctgatct tgatgattac acattctacg ttgctggcct tgttggcgtt
     6061 atgcttaacg atatctggaa atggtacgat ggcacagaaa cagataaaga acttgctatc
     6121 gctttcggcc gtggccttca atctgttaac atccttcgta acacatctga agattctgaa
     6181 cgtggcgttt ctttcttccc taacaactgg tctcgtgaag atatgttcgc ttacgctcgt
     6241 cgtaaccttt ctcttggcaa aaaatacctt gaagatgtta aatctcttcc tatccttcat
     6301 ttctgcaaaa tccctcttgc tcttgctgat ggcacactta acgctcttat gaaaggcaaa
     6361 gaaaaaatga cacgtgatga tgttaacaaa acagttaacg aagttgttga tatgggcgga
     6421 ggtggatctg gtggcggagg tagtggaggt gggggaagca tggcaagcga aaaagaaatc
     6481 agaagagaaa gatttctgaa cgtgtttccg aaactggttg aagaattaaa cgcgagcctg
     6541 ctggcgtatg gcatgccgaa agaagcatgc gattggtatg cacattcact gaattataat
     6601 acaccgggcg gcaaactgaa tcgcggcctg tcagtggttg atacgtatgc aattttatct
     6661 aataaaacgg tggaacaact gggacaagaa gaatatgaaa aagttgcaat cctgggctgg
     6721 tgcatcgaac tgctgcaggc atatttttta gtggcggatg atatgatgga taaaagcatc
     6781 acaagaagag gacaaccttg ctggtataaa gtgccggaag tgggagaaat tgcaattaat
     6841 gatgcgttta tgcttgaagc ggcgatttat aaactgctga aaagccattt tcgcaacgaa
     6901 aaatattata tcgatatcac ggaactgttt catgaagtta catttcagac agaactgggc
     6961 caattgatgg atctgattac agcaccggaa gataaagtgg atctttcaaa atttagcctg
     7021 aaaaagcatt catttatcgt gacatttaaa acggcatatt attcatttta tctgccggta
     7081 gccctggcaa tgtatgttgc aggcattaca gatgaaaaag atctgaaaca agcaagagat
     7141 gtgcttatcc cgctgggcga atattttcaa attcaagatg attatctgga ttgctttgga
     7201 acaccggaac agattggaaa aatcggcaca gatatccaag ataacaaatg ttcatgggtt
     7261 attaacaaag cactggaact ggcatcagca gaacaaagaa aaacactgga tgaaaattat
     7321 ggcaaaaagg atagcgttgc agaagcaaaa tgcaaaaaga tttttaacga tctgaaaatc
     7381 gaacagctgt atcatgaata tgaagaaagc atcgcaaaag atctgaaagc aaaaatttca
     7441 caagtggatg aatcaagagg atttaaagcg gatgtactga cagcctttct gaacaaagtg
     7501 tataaaagaa gcaaacatca tcatcatcat cattaactcc cacgcgtcag ctcctgtaca
     7561 ggatgagccg cgggcggccg cactcttcct ttttcaatat tattgaagca tttatcaggg
     7621 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     7681 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     7741 attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaagaat tgatcctcta
     7801 gcacaaaaga aaaacgaaat gatacaccaa tcagtgcaaa aaaagatata atgggagata
     7861 agacggttcg tgttcgtgct gacttgcacc atatcataaa aatcgaaaca gcaaagaatg
     7921 gcggaaacgt aaaagaagtt atggaaataa gacttagaag caaacttaag agtgtgttga
     7981 tagtgcagta tcttaaaatt ttgtataata ggaattgaag ttaaattaga tgctaaaaat
     8041 ttgtaattaa gaaggagtga ttacatgaac aaaaatataa aatattctca aaacttttta
     8101 acgagtgaaa aagtactcaa ccaaataata aaacaattga atttaaaaga aaccgatacc
     8161 gtttacgaaa ttggaacagg taaagggcat ttaacgacga aactggctaa aataagtaaa
     8221 caggtaacgt ctattgaatt agacagtcat ctattcaact tatcgtcaga aaaattaaaa
     8281 ctgaatactc gtgtcacttt aattcaccaa gatattctac agtttcaatt ccctaacaaa
     8341 cagaggtata aaattgttgg gagtattcct taccatttaa gcacacaaat tattaaaaaa
     8401 gtggtttttg aaagccatgc gtctgacatc tatctgattg ttgaagaagg attctacaag
     8461 cgtaccttgg atattcaccg aacactaggg ttgctcttgc acactcaagt ctcgattcag
     8521 caattgctta agctgccagc ggaatgcttt catcctaaac caaaagtaaa cagtgtctta
     8581 ataaaactta cccgccatac cacagatgtt ccagataaat attggaagct atatacgtac
     8641 tttgtttcaa aatgggtcaa tcgagaatat cgtcaactgt ttactaaaaa tcagtttcat
     8701 caagcaatga aacacgccaa agtaaacaat ttaagtaccg ttacttatga gcaagtattg
     8761 tctattttta atagttatct attatttaac gggaggaaat aattctatga gtcgcttttg
     8821 taaatttgga aagttacacg ttactaaagg gaatgtagat aaattattag gtatactact
     8881 gacagcttcc aaggagctaa agaggtccct agactctaga cccggggatc tctgcagtga
     8941 gatctggtaa tgactctcta gcttgaggca tcaaataaaa cgaaaggctc agtcgaaaga
     9001 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc
     9061 gccgctctag ctaagcagaa ggccatcctg acggatggcc tttttgcgtt tctacaaact
     9121 cttgttaact ctagagctgc ctgccgcgtt tcggtgatga agatcttccc gatgattaat
     9181 taattcagaa cgctcggttg ccgccgggcg ttttttatgc agcaatggca agaacgttgc
     9241 tctagagcgg ccgcatcgat tcacagtggc aatctccccc gtattcgttt gaaatgtgcc
     9301 acattaacag cgccgggtga tgtccgtatc gttctgctaa taagcggttg atgtgccgtg
     9361 ttttttctcg gtagacttta gatgtgaggc agtggttgtg ccttccgccg tgcagctgtt
     9421 tgacgcggga ggcattgacg cgcaaaactt ccggataggt ttgcgacagc caggccggac
     9481 gggctccgct cggcgttgct aatatgaccc ggccgcctat actgtgaatc cgctcaaaaa
     9541 tatcatccag ccattcaaat tgatatacgc cctcctccgg ctcaagtgcg ctccatgcaa
     9601 aaatgccgac agaaaacgta ttcgtatgag aaagcttcat cagtttgata tcgtcagcta
     9661 aaatatcggg ccgatccagc cactgatcgg ggttgtagtc tcccccatgg agcataaatt
     9721 ttgcttttgt tacgtgcgtt ttttcaagct ttgacatcac ccc
//
