LOCUS       40924_47388        7876 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pXY4, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7876)
  AUTHORS   Liu Y-H., Zhang X-X., Rainey PB.
  TITLE     Construction of mini-Tn7 vectors for studying gene expression in 
            Pseudomonas at post-transcriptional levels
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7876)
  AUTHORS   Liu Y-H., Zhang X-X., Rainey PB.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 7876)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7876)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-JUL-2014) Institute of Natural "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7876
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  382..580
                     /mobile_element_type="transposon:Tn7R"
                     /label=n7R
                     /note="right end of Tn7"
     primer_bind     585..601
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      629..723
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      826..912
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    958..1005
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(1138..1668)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     promoter        complement(1857..1885)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     misc_feature    1926..1973
                     /note="FRT; Flp recombinase target site"
     protein_bind    1926..1973
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     regulatory      2006..2483
                     /label=PdctA
                     /note="PdctA"
                     /regulatory_class="promoter"
     misc_feature    2457..2472
                     /label=sigma-54 binding site
                     /note="sigma-54 binding site"
     CDS             2478..5534
                     /label=lacZ
                     /note="beta-galactosidase"
     mobile_element  complement(5622..5787)
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      complement(6057..6645)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6819..7676)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7677..7781)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct agaggaccag ccgcgtaacc tggcaaaatc ggttacggtt gagtaataaa
      361 tggatgccct gcgtaagcgg gtgtgggcgg acaataaagt cttaaactga acaaaataga
      421 tctaaactat gacaataaag tcttaaacta gacagaatag ttgtaaactg aaatcagtcc
      481 agttatgctg tgaaaaagca tactggactt ttgttatggc taaagcaaac tcttcatttt
      541 ctgaagtgca aattgcccgt cgtattaaag aggggcgtgg ggttcgaggt cgacggtatc
      601 gataagctag cttaattagc tgagcttgga ctcctgttga tagatccagt aatgacctca
      661 gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag
      721 aatccaagct agactgcgat gagtggcagg gcggggcgta atttttttaa ggcagttatt
      781 ggtgccctta aacgcctggg gtaatgactc tctagcttga ggcatcaaat aaaacgaaag
      841 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
      901 agtaggacaa atccgccgct aggagcttgc ggcccggacg atcgaattgg ggatcttgaa
      961 gtacctattc cgaagttcct attctctaga aagtatagga acttcagagc gcttttgaag
     1021 ctgatgtgct taaaaactta ctcaatggaa ttctagatcg aattggccgc ggcgttgtga
     1081 caatttaccg aacaactccg cggccgggaa gccgatctcg gcttgaacga attgttaggt
     1141 ggcggtactt gggtcgatat caaagtgcat cacttcttcc cgtatgccca actttgtata
     1201 gagagccact gcgggatcgt caccgtaatc tgcttgcacg tagatcacat aagcaccaag
     1261 cgcgttggcc tcatgcttga ggagattgat gagcgcggtg gcaatgccct gcctccggtg
     1321 ctcgccggag actgcgagat catagatata gatctcacta cgcggctgct caaacttggg
     1381 cagaacgtaa gccgcgagag cgccaacaac cgcttcttgg tcgaaggcag caagcgcgat
     1441 gaatgtctta ctacggagca agttcccgag gtaatcggag tccggctgat gttgggagta
     1501 ggtggctacg tctccgaact cacgaccgaa aagatcaaga gcagcccgca tggatttgac
     1561 ttggtcaggg ccgagcctac atgtgcgaat gatgcccata cttgagccac ctaactttgt
     1621 tttagggcga ctgccctgct gcgtaacatc gttgctgctg cgtaacatcg ttgctgctcc
     1681 ataacatcaa acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag
     1741 actgtacaaa aaaacagtca taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc
     1801 tgcgttcggt caaggttctg gaccagttgc gtgagcgcat acgctacttg cattacagtt
     1861 tacgaaccga acaggcttat gtcaattcga tccattgctg ttgacaaagg gaatcagggg
     1921 atcttgaagt tcctattccg aagttcctat tctctagaaa gtataggaac ttcagagcgc
     1981 ttttgaagct aattcgatca tgcatggatc agcgcgtggc acatcatggc gaaggtcccg
     2041 cgtttgctcg ggccttcagc gaagcccagg tattcgtcgt ccagcgccag ccagagcatt
     2101 tgcagcagcc gggtaaattg ctccggcgcg atgcgggcgc ggggttcggt caacagttcc
     2161 ggggtgatgc ccacctgttg cagcaacccc gaatagtcgt aaccggcccg ccgcgccccg
     2221 ccgagggctg cccgggcgta atgactggcg atggtacgtt cacgcatgcg caggcctcgt
     2281 ccatggaagg acggatggta gccatcaccg cagagggtca caaggcggat atccgccaaa
     2341 ttgtaggacg ggattggacg ggtcggcgga aatccgccac gtgttgcggc gcttaggaag
     2401 gcgtctgcaa accccaagcc ctgatgtcta aaggccttta aggtttttca gcaaagtggc
     2461 acggcgtttg cgataaggat gacaagcttg tcgttttaca acgtcgtgac tgggaaaacc
     2521 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     2581 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc
     2641 gctttgcctg gtttccggca ccagaagcgg tgccggaaag ctggctggag tgcgatcttc
     2701 ctgaggccga tactgtcgtc gtcccctcaa actggcagat gcacggttac gatgcgccca
     2761 tctacaccaa cgtaacctat cccattacgg tcaatccgcc gtttgttccc acggagaatc
     2821 cgacgggttg ttactcgctc acatttaatg ttgatgaaag ctggctacag gaaggccaga
     2881 cgcgaattat ttttgatggc gttaactcgg cgtttcatct gtggtgcaac gggcgctggg
     2941 tcggttacgg ccaggacagt cgtttgccgt ctgaatttga cctgagcgca tttttacgcg
     3001 ccggagaaaa ccgcctcgcg gtgatggtgc tgcgttggag tgacggcagt tatctggaag
     3061 atcaggatat gtggcggatg agcggcattt tccgtgacgt ctcgttgctg cataaaccga
     3121 ctacacaaat cagcgatttc catgttgcca ctcgctttaa tgatgatttc agccgcgctg
     3181 tactggaggc tgaagttcag atgtgcggcg agttgcgtga ctacctacgg gtaacagttt
     3241 ctttatggca gggtgaaacg caggtcgcca gcggcaccgc gcctttcggc ggtgaaatta
     3301 tcgatgagcg tggtggttat gccgatcgcg tcacactacg tctgaacgtc gaaaacccga
     3361 aactgtggag cgccgaaatc ccgaatctct atcgtgcggt ggttgaactg cacaccgccg
     3421 acggcacgct gattgaagca gaagcctgcg atgtcggttt ccgcgaggtg cggattgaaa
     3481 atggtctgct gctgctgaac ggcaagccgt tgctgattcg aggcgttaac cgtcacgagc
     3541 atcatcctct gcatggtcag gtcatggatg agcagacgat ggtgcaggat atcctgctga
     3601 tgaagcagaa caactttaac gccgtgcgct gttcgcatta tccgaaccat ccgctgtggt
     3661 acacgctgtg cgaccgctac ggcctgtatg tggtggatga agccaatatt gaaacccacg
     3721 gcatggtgcc aatgaatcgt ctgaccgatg atccgcgctg gctaccggcg atgagcgaac
     3781 gcgtaacgcg aatggtgcag cgcgatcgta atcacccgag tgtgatcatc tggtcgctgg
     3841 ggaatgaatc aggccacggc gctaatcacg acgcgctgta tcgctggatc aaatctgtcg
     3901 atccttcccg cccggtgcag tatgaaggcg gcggagccga caccacggcc accgatatta
     3961 tttgcccgat gtacgcgcgc gtggatgaag accagccctt cccggctgtg ccgaaatggt
     4021 ccatcaaaaa atggctttcg ctacctggag agacgcgccc gctgatcctt tgcgaatacg
     4081 cccacgcgat gggtaacagt cttggcggtt tcgctaaata ctggcaggcg tttcgtcagt
     4141 atccccgttt acagggcggc ttcgtctggg actgggtgga tcagtcgctg attaaatatg
     4201 atgaaaacgg caacccgtgg tcggcttacg gcggtgattt tggcgatacg ccgaacgatc
     4261 gccagttctg tatgaacggt ctggtctttg ccgaccgcac gccgcatcca gcgctgacgg
     4321 aagcaaaaca ccagcagcag tttttccagt tccgtttatc cgggcaaacc atcgaagtga
     4381 ccagcgaata cctgttccgt catagcgata acgagctcct gcactggatg gtggcgctgg
     4441 atggtaagcc gctggcaagc ggtgaagtgc ctctggatgt cgctccacaa ggtaaacagt
     4501 tgattgaact gcctgaacta ccgcagccgg agagcgccgg gcaactctgg ctcacagtac
     4561 gcgtagtgca accgaacgcg accgcatggt cagaagccgg gcacatcagc gcctggcagc
     4621 agtggcgtct ggcggaaaac ctcagtgtga cgctccccgc cgcgtcccac gccatcccgc
     4681 atctgaccac cagcgaaatg gatttttgca tcgagctggg taataagcgt tggcaattta
     4741 accgccagtc aggctttctt tcacagatgt ggattggcga taaaaaacaa ctgctgacgc
     4801 cgctgcgcga tcagttcacc cgtgcaccgc tggataacga cattggcgta agtgaagcga
     4861 cccgcattga ccctaacgcc tgggtcgaac gctggaaggc ggcgggccat taccaggccg
     4921 aagcagcgtt gttgcagtgc acggcagata cacttgctga tgcggtgctg attacgaccg
     4981 ctcacgcgtg gcagcatcag gggaaaacct tatttatcag ccggaaaacc taccggattg
     5041 atggtagtgg tcaaatggcg attaccgttg atgttgaagt ggcgagcgat acaccgcatc
     5101 cggcgcggat tggcctgaac tgccagctgg cgcaggtagc agagcgggta aactggctcg
     5161 gattagggcc gcaagaaaac tatcccgacc gccttactgc cgcctgtttt gaccgctggg
     5221 atctgccatt gtcagacatg tataccccgt acgtcttccc gagcgaaaac ggtctgcgct
     5281 gcgggacgcg cgaattgaat tatggcccac accagtggcg cggcgacttc cagttcaaca
     5341 tcagccgcta cagtcaacag caactgatgg aaaccagcca tcgccatctg ctgcacgcgg
     5401 aagaaggcac atggctgaat atcgacggtt tccatatggg gattggtggc gacgactcct
     5461 ggagcccgtc agtatcggcg gagttccagc tgagcgccgg tcgctaccat taccagttgg
     5521 tctggtgtca aaaataataa taaccgggca ggccatgtct gcccgtattt cgcgtaagga
     5581 aatccattat gtactattta aaatcaagct ccttgcaggc caaccagata agtgaaatct
     5641 agttccaaac tattttgtca tttttaattt tcgtattagc ttacgacgct acacccagtt
     5701 cccatctatt ttgtcactct tccctaaata atccttaaaa actccatttc cacccctccc
     5761 agttcccaac tattttgtcc gcccacagcg gggcattttt cttcctgtta tgtttgggcg
     5821 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     5881 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     5941 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     6001 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     6061 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     6121 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     6181 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     6241 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     6301 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     6361 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     6421 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     6481 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     6541 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     6601 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     6661 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     6721 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     6781 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     6841 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     6901 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     6961 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     7021 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     7081 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     7141 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     7201 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     7261 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     7321 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     7381 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     7441 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     7501 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     7561 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     7621 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     7681 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     7741 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     7801 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     7861 acgaggccct ttcgtc
//
