LOCUS       40924_47373        7391 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pXY1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7391)
  AUTHORS   Liu Y-H., Zhang X-X., Rainey PB.
  TITLE     Construction of mini-Tn7 vectors for studying gene expression in 
            Pseudomonas at post-transcriptional levels
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7391)
  AUTHORS   Liu Y-H., Zhang X-X., Rainey PB.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 7391)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7391)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-JUL-2014) Institute of Natural "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7391
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  382..580
                     /mobile_element_type="transposon:Tn7R"
                     /label=n7R
                     /note="Tn7R; right end of Tn7"
     primer_bind     585..601
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      629..723
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      826..912
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    958..1005
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(1138..1668)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     promoter        complement(1857..1885)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     misc_feature    1926..1973
                     /note="FRT; Flp recombinase target site"
     protein_bind    1926..1973
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             2031..5084
                     /label=lacZ
                     /note="beta-galactosidase"
     mobile_element  complement(5167..5332)
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      5538..5922
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     CDS             complement(6334..7191)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7192..7296)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct agaggaccag ccgcgtaacc tggcaaaatc ggttacggtt gagtaataaa
      361 tggatgccct gcgtaagcgg gtgtgggcgg acaataaagt cttaaactga acaaaataga
      421 tctaaactat gacaataaag tcttaaacta gacagaatag ttgtaaactg aaatcagtcc
      481 agttatgctg tgaaaaagca tactggactt ttgttatggc taaagcaaac tcttcatttt
      541 ctgaagtgca aattgcccgt cgtattaaag aggggcgtgg ggttcgaggt cgacggtatc
      601 gataagctag cttaattagc tgagcttgga ctcctgttga tagatccagt aatgacctca
      661 gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag
      721 aatccaagct agactgcgat gagtggcagg gcggggcgta atttttttaa ggcagttatt
      781 ggtgccctta aacgcctggg gtaatgactc tctagcttga ggcatcaaat aaaacgaaag
      841 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
      901 agtaggacaa atccgccgct aggagcttgc ggcccggacg atcgaattgg ggatcttgaa
      961 gtacctattc cgaagttcct attctctaga aagtatagga acttcagagc gcttttgaag
     1021 ctgatgtgct taaaaactta ctcaatggaa ttctagatcg aattggccgc ggcgttgtga
     1081 caatttaccg aacaactccg cggccgggaa gccgatctcg gcttgaacga attgttaggt
     1141 ggcggtactt gggtcgatat caaagtgcat cacttcttcc cgtatgccca actttgtata
     1201 gagagccact gcgggatcgt caccgtaatc tgcttgcacg tagatcacat aagcaccaag
     1261 cgcgttggcc tcatgcttga ggagattgat gagcgcggtg gcaatgccct gcctccggtg
     1321 ctcgccggag actgcgagat catagatata gatctcacta cgcggctgct caaacctggg
     1381 cagaacgtaa gccgcgagag cgccaacaac cgcttcttgg tcgaaggcag caagcgcgat
     1441 gaatgtctta ctacggagca agttcccgag gtaatcggag tccggctgat gttgggagta
     1501 ggtggctacg tctccgaact cacgaccgaa aagatcaaga gcagcccgca tggatttgac
     1561 ttggtcaggg ccgagcctac atgtgcgaat gatgcccata cttgagccac ctaactttgt
     1621 tttagggcga ctgccctgct gcgtaacatc gttgctgctg cgtaacatcg ttgctgctcc
     1681 ataacatcaa acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag
     1741 actgtacaaa aaaacagtca taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc
     1801 tgcgttcggt caaggttctg gaccagttgc gtgagcgcat acgctacttg cattacagtt
     1861 tacgaaccga acaggcttat gtcaattcga tccattgctg ttgacaaagg gaatcagggg
     1921 atcttgaagt tcctattccg aagttcctat tctctagaaa gtataggaac ttcagagcgc
     1981 ttttgaagct aattcgatca tgcatgagct cactagtccc ggctgcagga tccaagcttg
     2041 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     2101 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     2161 aacagttgcg cagcctgaat ggcgaatggc gctttgcctg gtttccggca ccagaagcgg
     2221 tgccggaaag ctggctggag tgcgatcttc ctgaggccga tactgtcgtc gtcccctcaa
     2281 actggcagat gcacggttac gatgcgccca tctacaccaa cgtgacctat cccattacgg
     2341 tcaatccgcc gtttgttccc acggagaatc cgacgggttg ttactcgctc acatttaatg
     2401 ttgatgaaag ctggctacag gaaggccaga cgcgaattat ttttgatggc gttaactcgg
     2461 cgtttcatct gtggtgcaac gggcgctggg tcggttacgg ccaggacagt cgtttgccgt
     2521 ctgaatttga cctgagcgca tttttacgcg ccggagaaaa ccgcctcgcg gtgatggtgc
     2581 tgcgctggag tgacggcagt tatctggaag atcaggatat gtggcggatg agcggcattt
     2641 tccgtgacgt ctcgttgctg cataaaccga ctacacaaat cagcgatttc catgttgcca
     2701 ctcgctttaa tgatgatttc agccgcgctg tactggaggc tgaagttcag atgtgcggcg
     2761 agttgcgtga ctacctacgg gtaacagttt ctttatggca gggtgaaacg caggtcgcca
     2821 gcggcaccgc gcctttcggc ggtgaaatta tcgatgagcg tggtggttat gccgatcgcg
     2881 tcacactacg tctgaacgtc gaaaacccga aactgtggag cgccgaaatc ccgaatctct
     2941 atcgtgcggt ggttgaactg cacaccgccg acggcacgct gattgaagca gaagcctgcg
     3001 atgtcggttt ccgcgaggtg cggattgaaa atggtctgct gctgctgaac ggcaagccgt
     3061 tgctgattcg aggcgttaac cgtcacgagc atcatcctct gcatggtcag gtcatggatg
     3121 agcagacgat ggtgcaggat atcctgctga tgaagcagaa caactttaac gccgtgcgct
     3181 gttcgcatta tccgaaccat ccgctgtggt acacgctgtg cgaccgctac ggcctgtatg
     3241 tggtggatga agccaatatt gaaacccacg gcatggtgcc aatgaatcgt ctgaccgatg
     3301 atccgcgctg gctaccggcg atgagcgaac gcgtaacgcg aatggtgcag cgcgatcgta
     3361 atcacccgag tgtgatcatc tggtcgctgg ggaatgaatc aggccacggc gctaatcacg
     3421 acgcgctgta tcgctggatc aaatctgtcg atccttcccg cccggtgcag tatgaaggcg
     3481 gcggagccga caccacggcc accgatatta tttgcccgat gtacgcgcgc gtggatgaag
     3541 accagccctt cccggctgtg ccgaaatggt ccatcaaaaa atggctttcg ctacctggag
     3601 agacgcgccc gctgatcctt tgcgaatacg cccacgcgat gggtaacagt cttggcggtt
     3661 tcgctaaata ctggcaggcg tttcgtcagt atccccgttt acagggcggc ttcgtctggg
     3721 actgggtgga tcagtcgctg attaaatatg atgaaaacgg caacccgtgg tcggcttacg
     3781 gcggtgattt tggcgatacg ccgaacgatc gccagttctg tatgaacggt ctggtctttg
     3841 ccgaccgcac gccgcatcca gcgctgacgg aagcaaaaca ccagcagcag tttttccagt
     3901 tccgtttatc cgggcaaacc atcgaagtga ccagcgaata cctgttccgt catagcgata
     3961 acgagctcct gcactggatg gtggcgctgg atggtaagcc gctggcaagc ggtgaagtgc
     4021 ctctggatgt cgctccacaa ggtaaacagt tgattgaact gcctgaacta ccgcagccgg
     4081 agagcgccgg gcaactctgg ctcacagtac gcgtagtgca accgaacgcg accgcatggt
     4141 cagaagccgg gcacatcagc gcctggcagc agtggcgtct ggcggaaaac ctcagtgtga
     4201 cgctccccgc cgcgtcccac gccatcccgc atctgaccac cagcgaaatg gatttttgca
     4261 tcgagctggg taataagcgt tggcaattta accgccagtc aggctttctt tcacagatgt
     4321 ggattggcga taaaaaacaa ctgctgacgc cgctgcgcga tcagttcacc cgtgcaccgc
     4381 tggataacga cattggcgta agtgaagcga cccgcattga ccctaacgcc tgggtcgaac
     4441 gctggaaggc ggcgggccat taccaggccg aagcagcgtt gttgcagtgc acggcagata
     4501 cacttgctga tgcggtgctg attacgaccg ctcacgcgtg gcagcatcag gggaaaacct
     4561 tatttatcag ccggaaaacc taccggattg atggtagtgg tcaaatggcg attaccgttg
     4621 atgttgaagt ggcgagcgat acaccgcatc cggcgcggat tggcctgaac tgccagctgg
     4681 cgcaggtagc agagcgggta aactggctcg gattagggcc gcaagaaaac tatcccgacc
     4741 gccttactgc cgcctgtttt gaccgctggg atctgccatt gtcagacatg tataccccgt
     4801 acgtcttccc gagcgaaaac ggtctgcgct gcgggacgcg cgaattgaat tatggcccac
     4861 accagtggcg cggcgacttc cagttcaaca tcagccgcta cagtcaacag caactgatgg
     4921 aaaccagcca tcgccatctg ctgcacgcgg aagaaggcac atggctgaat atcgacggtt
     4981 tccatatggg gattggtggc gacgactcct ggagcccgtc agtatcggcg gaattacagc
     5041 tgagcgccgg tcgctaccat taccagttgg tctggtgtca aaaataataa taaccgggca
     5101 ggccatgtct gcccgtattt cgcgtaagga aatccattat ggtacctcgc gaaggccttg
     5161 caggccaacc agataagtga aatctagttc caaactattt tgtcattttt aattttcgta
     5221 ttagcttacg acgctacacc cagttcccat ctattttgtc actcttccct aaataatcct
     5281 taaaaactcc atttccaccc ctcccagttc ccaactattt tgtccgccca cagcggggca
     5341 tttttcttcc tgttatgttt gggcgctgca ttaatgaatc ggccaacgcg cggggagagg
     5401 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gacccgctgc gctcggtcgt
     5461 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     5521 aggggataac gcaggaaaga acatgggcag ttcaacctgt tgatagtacg tactaagctc
     5581 tcatgtttca cgtactaagc tctcaagttt aacgtactaa gctctcatgt ttaacgaact
     5641 aaaccctcat ggctaacgta ctaagctctc atggctaacg tactaagctc tcatgtttca
     5701 cgtactaagc tctcatgttt gaacaataaa attaatataa atcagcaact taaatagcct
     5761 ctaaggtttt aagttttata agaaaaaaaa gaatatataa ggcttttaaa gcttttaagg
     5821 tttaacggtt gtggacaaca agccagggat gtaacgcact gagaagccct tagagcctct
     5881 caaagcaatt ttgagtgaca caggaacact taacggctga caaccggctg gtaacaggat
     5941 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     6001 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     6061 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     6121 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     6181 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     6241 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     6301 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     6361 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     6421 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     6481 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     6541 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     6601 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     6661 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     6721 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     6781 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     6841 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     6901 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     6961 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     7021 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     7081 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     7141 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     7201 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     7261 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     7321 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     7381 gccctttcgt c
//
