LOCUS       40924_47368        5632 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Transient expression vector pXUN, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5632)
  AUTHORS   Chen S, Songkumarn P, Liu J, Wang GL.
  TITLE     A versatile zero background T-vector system for gene cloning and 
            functional genomics
  JOURNAL   Plant Physiol. 150 (3), 1111-1121 (2009)
  PUBMED    19403729
REFERENCE   2  (bases 1 to 5632)
  AUTHORS   Chen S, Songkumarn P, Liu J, Wang G-L.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2009) Department of Plant Pathology, The Ohio 
            State University, 201 Kottman Hall, 2021 Coffey Rd, Columbus, OH 
            43210, USA
REFERENCE   3  (bases 1 to 5632)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5632)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2009"; volume: "150"; issue: "3"; pages: 
            "1111-1121"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-APR-2009) Department of Plant Pathology, The Ohio State 
            University, 201 Kottman Hall, 2021 Coffey Rd, Columbus, OH 43210, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5632
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(9..261)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             622..924
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     promoter        complement(995..2985)
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     primer_bind     complement(3016..3032)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3040..3056)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3064..3094)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3109..3130)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3418..4006)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4180..5037)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5038..5142)
                     /label=AmpR promoter
     primer_bind     5616..5632
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattcccga tctagtaaca tagatgacac cgcgcgcgat aatttatcct agtttgcgcg
       61 ctatattttg ttttctatcg cgtattaaat gtataattgc gggactctaa tcataaaaac
      121 ccatctcata aataacgtca tgcattacat gttaattatt acatgcttaa cgtaattcaa
      181 cagaaattat atgataatca tcgcaagacc ggcaacagga ttcaatctta agaaacttta
      241 ttgccaaatg tttgaacgat cggggaaatt cggatcccca atacttgtat ggggcttact
      301 aaaagccaga taacagtatg cgtatttgcg cgctgatttt tgcggtataa gaatatatac
      361 tgatatgtat acccgaagta tgtcaaaaag aggtatgcta tgaagcagcg tattacagtg
      421 acagttgaca gcgacagcta tcagttgctc aaggcatata tgatgtcaat atctccggtc
      481 tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg
      541 aaaatcagga agggatggct gaggtcgccc ggtttattga aatgaacggc tcttttgctg
      601 acgagaacag gggctggtga aatgcagttt aaggtttaca cctataaaag agagagccgt
      661 tatcgtctgt ttgtggatgt acagagtgat attattgaca cgcccgggcg acggatggtg
      721 atccccctgg ccagtgcacg tctgctgtca gataaagtct cccgtgaact ttacccggtg
      781 gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtc
      841 tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc
      901 attaacctga tgttctgggg aatataaatg tcaggctccc ttatacacag ccagtctgca
      961 ggtcgaccca tacaagtatt ggggatcccc cgggctgcag aagtaacacc aaacaacagg
     1021 gtgagcatcg acaaaagaaa cagtaccaag caaataaata gcgtatgaag gcagggctaa
     1081 aaaaatccac atatagctgc tgcatatgcc atcatccaag tatatcaaga tcaaaataat
     1141 tataaaacat acttgtttat tataatagat aggtactcaa ggttagagca tatgaataga
     1201 tgctgcatat gccatcatgt atatgcatca gtaaaaccca catcaacatg tatacctatc
     1261 ctagatcgat atttccatcc atcttaaact cgtaactatg aagatgtatg acacacacat
     1321 acagttccaa aattaataaa tacaccaggt agtttgaaac agtattctac tccgatctag
     1381 aacgaatgaa cgaccgccca accacaccac atcatcacaa ccaagcgaac aaaaagcatc
     1441 tctgtatatg catcagtaaa acccgcatca acatgtatac ctatcctaga tcgatatttc
     1501 catccatcat cttcaattcg taactatgaa tatgtatggc acacacatac agatccaaaa
     1561 ttaataaatc caccaggtag tttgaaacag aattctactc cgatctagaa cgaccgccca
     1621 accagaccac atcatcacaa ccaagacaaa aaaaagcatg aaaagatgac ccgacaaaca
     1681 agtgcacggc atatattgaa ataaaggaaa agggcaaacc aaaccctatg caacgaaaca
     1741 aaaaaaatca tgaaatcgat cccgtctgcg gaacggctag agccatccca ggattcccca
     1801 aagagaaaca ctggcaagtt agcaatcaga acgtgtctga cgtacaggtc gcatccgtgt
     1861 acgaacgcta gcagcacgga tctaacacaa acacggatct aacacaaaca tgaacagaag
     1921 tagaactacc gggccctaac catggaccgg aacgccgatc tagagaaggt agagaggggg
     1981 ggggggggag gacgagcggc gtaccttgaa gcggaggtgc cgacgggtgg atttggggga
     2041 gatctggttg tgtgtgtgtg cgctccgaac aacacgaggt tggggaaaga gggtgtggag
     2101 ggggtgtcta tttattacgg cgggcgagga agggaaagcg aaggagcggt gggaaaggaa
     2161 tcccccgtag ctgccgtgcc gtgagaggag gaggaggccg cctgccgtgc cggctcacgt
     2221 ctgccgctcc gccacgcatt tctggatgcc gacagcggag caagtccaac ggtggagcgg
     2281 aactctcgag aggggtccag aggcagcgac agagatgccg tgccgtctgc ttcgcttggc
     2341 ccgacgcgac gctgctggtt cgctggttgg tgtccgttag actcgtcgac ggcgtttaac
     2401 aggctggcat tatctactcg aaacaagaaa aatgtttcct tagttttttt aatttcttaa
     2461 agggtatttg tttaattttt agtcacttta ttttattcta ttttatatct aaattattaa
     2521 ataaaaaaac taaaatagag ttttagtttt cttaatttag aggctaaaat agaataaaat
     2581 agatgtacta aaaaaattag tctataaaaa ccattaaccc taaaccctaa atggatgtac
     2641 taataaaatg gatgaagtat tatataggtg aagctatttg caaaaaaaaa ggagaacaca
     2701 tgcacactaa aaagataaaa ctgtagagtc ctgttgtcaa aatactcaat tgtcctttag
     2761 accatgtcta actgttcatt tatatgattc tctaaaacac tgatattatt gtagtactat
     2821 agattatatt attcgtagag taaagtttaa atatatgtat aaagatagat aaactgcact
     2881 tcaaacaagt gtgacaaaaa aaatatgtgg taatttttta taacttagac atgcaatgct
     2941 cattatctct agagaggggc acgaccgggt cacgctgcac tgcaggaatt cgatatcaag
     3001 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     3061 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     3121 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     3181 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     3241 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     3301 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     3361 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     3421 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     3481 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     3541 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     3601 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     3661 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     3721 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     3781 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     3841 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     3901 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     3961 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     4021 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     4081 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     4141 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     4201 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     4261 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     4321 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     4381 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     4441 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     4501 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     4561 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     4621 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     4681 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     4741 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     4801 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     4861 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     4921 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     4981 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     5041 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     5101 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     5161 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     5221 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     5281 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     5341 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
     5401 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     5461 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     5521 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
     5581 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gt
//
