LOCUS       40924_47363        5665 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Transient expression vector pXUN-Myc, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Chen S, Songkumarn P, Liu J, Wang GL.
  TITLE     A versatile zero background T-vector system for gene cloning and 
            functional genomics
  JOURNAL   Plant Physiol. 150 (3), 1111-1121 (2009)
  PUBMED    19403729
REFERENCE   2  (bases 1 to 5665)
  AUTHORS   Chen S, Songkumarn P, Liu J, Wang G-L.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2009) Department of Plant Pathology, The Ohio 
            State University, 201 Kottman Hall, 2021 Coffey Rd, Columbus, OH 
            43210, USA
REFERENCE   3  (bases 1 to 5665)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5665)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2009"; volume: "150"; issue: "3"; pages: 
            "1111-1121"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-APR-2009) Department of Plant Pathology, The Ohio State 
            University, 201 Kottman Hall, 2021 Coffey Rd, Columbus, OH 43210, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5665
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(9..261)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             622..924
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(983..1012)
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
     promoter        complement(1028..3018)
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     primer_bind     complement(3049..3065)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3073..3089)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3097..3127)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3142..3163)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3451..4039)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4213..5070)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5071..5175)
                     /label=AmpR promoter
     primer_bind     5649..5665
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattcccga tctagtaaca tagatgacac cgcgcgcgat aatttatcct agtttgcgcg
       61 ctatattttg ttttctatcg cgtattaaat gtataattgc gggactctaa tcataaaaac
      121 ccatctcata aataacgtca tgcattacat gttaattatt acatgcttaa cgtaattcaa
      181 cagaaattat atgataatca tcgcaagacc ggcaacagga ttcaatctta agaaacttta
      241 ttgccaaatg tttgaacgat cggggaaatt cggatcccca atacttgtat ggggcttact
      301 aaaagccaga taacagtatg cgtatttgcg cgctgatttt tgcggtataa gaatatatac
      361 tgatatgtat acccgaagta tgtcaaaaag aggtatgcta tgaagcagcg tattacagtg
      421 acagttgaca gcgacagcta tcagttgctc aaggcatata tgatgtcaat atctccggtc
      481 tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg
      541 aaaatcagga agggatggct gaggtcgccc ggtttattga aatgaacggc tcttttgctg
      601 acgagaacag gggctggtga aatgcagttt aaggtttaca cctataaaag agagagccgt
      661 tatcgtctgt ttgtggatgt acagagtgat attattgaca cgcccgggcg acggatggtg
      721 atccccctgg ccagtgcacg tctgctgtca gataaagtct cccgtgaact ttacccggtg
      781 gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtc
      841 tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc
      901 attaacctga tgttctgggg aatataaatg tcaggctccc ttatacacag ccagtctgca
      961 ggtcgaccca tacaagtatt ggaagatctt cttcagaaat caacttttgt tccatggatc
     1021 ccccgggctg cagaagtaac accaaacaac agggtgagca tcgacaaaag aaacagtacc
     1081 aagcaaataa atagcgtatg aaggcagggc taaaaaaatc cacatatagc tgctgcatat
     1141 gccatcatcc aagtatatca agatcaaaat aattataaaa catacttgtt tattataata
     1201 gataggtact caaggttaga gcatatgaat agatgctgca tatgccatca tgtatatgca
     1261 tcagtaaaac ccacatcaac atgtatacct atcctagatc gatatttcca tccatcttaa
     1321 actcgtaact atgaagatgt atgacacaca catacagttc caaaattaat aaatacacca
     1381 ggtagtttga aacagtattc tactccgatc tagaacgaat gaacgaccgc ccaaccacac
     1441 cacatcatca caaccaagcg aacaaaaagc atctctgtat atgcatcagt aaaacccgca
     1501 tcaacatgta tacctatcct agatcgatat ttccatccat catcttcaat tcgtaactat
     1561 gaatatgtat ggcacacaca tacagatcca aaattaataa atccaccagg tagtttgaaa
     1621 cagaattcta ctccgatcta gaacgaccgc ccaaccagac cacatcatca caaccaagac
     1681 aaaaaaaagc atgaaaagat gacccgacaa acaagtgcac ggcatatatt gaaataaagg
     1741 aaaagggcaa accaaaccct atgcaacgaa acaaaaaaaa tcatgaaatc gatcccgtct
     1801 gcggaacggc tagagccatc ccaggattcc ccaaagagaa acactggcaa gttagcaatc
     1861 agaacgtgtc tgacgtacag gtcgcatccg tgtacgaacg ctagcagcac ggatctaaca
     1921 caaacacgga tctaacacaa acatgaacag aagtagaact accgggccct aaccatggac
     1981 cggaacgccg atctagagaa ggtagagagg gggggggggg gaggacgagc ggcgtacctt
     2041 gaagcggagg tgccgacggg tggatttggg ggagatctgg ttgtgtgtgt gtgcgctccg
     2101 aacaacacga ggttggggaa agagggtgtg gagggggtgt ctatttatta cggcgggcga
     2161 ggaagggaaa gcgaaggagc ggtgggaaag gaatcccccg tagctgccgt gccgtgagag
     2221 gaggaggagg ccgcctgccg tgccggctca cgtctgccgc tccgccacgc atttctggat
     2281 gccgacagcg gagcaagtcc aacggtggag cggaactctc gagaggggtc cagaggcagc
     2341 gacagagatg ccgtgccgtc tgcttcgctt ggcccgacgc gacgctgctg gttcgctggt
     2401 tggtgtccgt tagactcgtc gacggcgttt aacaggctgg cattatctac tcgaaacaag
     2461 aaaaatgttt ccttagtttt tttaatttct taaagggtat ttgtttaatt tttagtcact
     2521 ttattttatt ctattttata tctaaattat taaataaaaa aactaaaata gagttttagt
     2581 tttcttaatt tagaggctaa aatagaataa aatagatgta ctaaaaaaat tagtctataa
     2641 aaaccattaa ccctaaaccc taaatggatg tactaataaa atggatgaag tattatatag
     2701 gtgaagctat ttgcaaaaaa aaaggagaac acatgcacac taaaaagata aaactgtaga
     2761 gtcctgttgt caaaatactc aattgtcctt tagaccatgt ctaactgttc atttatatga
     2821 ttctctaaaa cactgatatt attgtagtac tatagattat attattcgta gagtaaagtt
     2881 taaatatatg tataaagata gataaactgc acttcaaaca agtgtgacaa aaaaaatatg
     2941 tggtaatttt ttataactta gacatgcaat gctcattatc tctagagagg ggcacgaccg
     3001 ggtcacgctg cactgcagga attcgatatc aagcttggcg taatcatggt catagctgtt
     3061 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     3121 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     3181 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     3241 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     3301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     3361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     3421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     3481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     3541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     3601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     3661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     3721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     3781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     3841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     3901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     3961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     4021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     4081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     4141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     4201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     4261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     4321 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     4381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     4441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     4501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     4561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     4621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     4681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     4741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     4801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     4861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     4921 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     4981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     5041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     5101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     5161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     5221 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt
     5281 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
     5341 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
     5401 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     5461 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt
     5521 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     5581 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
     5641 acgacgttgt aaaacgacgg ccagt
//
