LOCUS       40924_47358        5662 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Transient expression vector pXUN-HA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5662)
  AUTHORS   Chen S, Songkumarn P, Liu J, Wang GL.
  TITLE     A versatile zero background T-vector system for gene cloning and 
            functional genomics
  JOURNAL   Plant Physiol. 150 (3), 1111-1121 (2009)
  PUBMED    19403729
REFERENCE   2  (bases 1 to 5662)
  AUTHORS   Chen S, Songkumarn P, Liu J, Wang G-L.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2009) Department of Plant Pathology, The Ohio 
            State University, 201 Kottman Hall, 2021 Coffey Rd, Columbus, OH 
            43210, USA
REFERENCE   3  (bases 1 to 5662)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5662)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2009"; volume: "150"; issue: "3"; pages: 
            "1111-1121"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-APR-2009) Department of Plant Pathology, The Ohio State 
            University, 201 Kottman Hall, 2021 Coffey Rd, Columbus, OH 43210, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5662
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(9..261)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             622..924
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(983..1009)
                     /codon_start=1
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
     promoter        complement(1025..3015)
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     primer_bind     complement(3046..3062)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3070..3086)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3094..3124)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3139..3160)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3448..4036)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4210..5067)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5068..5172)
                     /label=AmpR promoter
     primer_bind     5646..5662
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattcccga tctagtaaca tagatgacac cgcgcgcgat aatttatcct agtttgcgcg
       61 ctatattttg ttttctatcg cgtattaaat gtataattgc gggactctaa tcataaaaac
      121 ccatctcata aataacgtca tgcattacat gttaattatt acatgcttaa cgtaattcaa
      181 cagaaattat atgataatca tcgcaagacc ggcaacagga ttcaatctta agaaacttta
      241 ttgccaaatg tttgaacgat cggggaaatt cggatcccca atacttgtat ggggcttact
      301 aaaagccaga taacagtatg cgtatttgcg cgctgatttt tgcggtataa gaatatatac
      361 tgatatgtat acccgaagta tgtcaaaaag aggtatgcta tgaagcagcg tattacagtg
      421 acagttgaca gcgacagcta tcagttgctc aaggcatata tgatgtcaat atctccggtc
      481 tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg
      541 aaaatcagga agggatggct gaggtcgccc ggtttattga aatgaacggc tcttttgctg
      601 acgagaacag gggctggtga aatgcagttt aaggtttaca cctataaaag agagagccgt
      661 tatcgtctgt ttgtggatgt acagagtgat attattgaca cgcccgggcg acggatggtg
      721 atccccctgg ccagtgcacg tctgctgtca gataaagtct cccgtgaact ttacccggtg
      781 gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtc
      841 tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc
      901 attaacctga tgttctgggg aatataaatg tcaggctccc ttatacacag ccagtctgca
      961 ggtcgaccca tacaagtatt ggagcgtaat ctggaacatc gtatgggtac atggatcccc
     1021 cgggctgcag aagtaacacc aaacaacagg gtgagcatcg acaaaagaaa cagtaccaag
     1081 caaataaata gcgtatgaag gcagggctaa aaaaatccac atatagctgc tgcatatgcc
     1141 atcatccaag tatatcaaga tcaaaataat tataaaacat acttgtttat tataatagat
     1201 aggtactcaa ggttagagca tatgaataga tgctgcatat gccatcatgt atatgcatca
     1261 gtaaaaccca catcaacatg tatacctatc ctagatcgat atttccatcc atcttaaact
     1321 cgtaactatg aagatgtatg acacacacat acagttccaa aattaataaa tacaccaggt
     1381 agtttgaaac agtattctac tccgatctag aacgaatgaa cgaccgccca accacaccac
     1441 atcatcacaa ccaagcgaac aaaaagcatc tctgtatatg catcagtaaa acccgcatca
     1501 acatgtatac ctatcctaga tcgatatttc catccatcat cttcaattcg taactatgaa
     1561 tatgtatggc acacacatac agatccaaaa ttaataaatc caccaggtag tttgaaacag
     1621 aattctactc cgatctagaa cgaccgccca accagaccac atcatcacaa ccaagacaaa
     1681 aaaaagcatg aaaagatgac ccgacaaaca agtgcacggc atatattgaa ataaaggaaa
     1741 agggcaaacc aaaccctatg caacgaaaca aaaaaaatca tgaaatcgat cccgtctgcg
     1801 gaacggctag agccatccca ggattcccca aagagaaaca ctggcaagtt agcaatcaga
     1861 acgtgtctga cgtacaggtc gcatccgtgt acgaacgcta gcagcacgga tctaacacaa
     1921 acacggatct aacacaaaca tgaacagaag tagaactacc gggccctaac catggaccgg
     1981 aacgccgatc tagagaaggt agagaggggg ggggggggag gacgagcggc gtaccttgaa
     2041 gcggaggtgc cgacgggtgg atttggggga gatctggttg tgtgtgtgtg cgctccgaac
     2101 aacacgaggt tggggaaaga gggtgtggag ggggtgtcta tttattacgg cgggcgagga
     2161 agggaaagcg aaggagcggt gggaaaggaa tcccccgtag ctgccgtgcc gtgagaggag
     2221 gaggaggccg cctgccgtgc cggctcacgt ctgccgctcc gccacgcatt tctggatgcc
     2281 gacagcggag caagtccaac ggtggagcgg aactctcgag aggggtccag aggcagcgac
     2341 agagatgccg tgccgtctgc ttcgcttggc ccgacgcgac gctgctggtt cgctggttgg
     2401 tgtccgttag actcgtcgac ggcgtttaac aggctggcat tatctactcg aaacaagaaa
     2461 aatgtttcct tagttttttt aatttcttaa agggtatttg tttaattttt agtcacttta
     2521 ttttattcta ttttatatct aaattattaa ataaaaaaac taaaatagag ttttagtttt
     2581 cttaatttag aggctaaaat agaataaaat agatgtacta aaaaaattag tctataaaaa
     2641 ccattaaccc taaaccctaa atggatgtac taataaaatg gatgaagtat tatataggtg
     2701 aagctatttg caaaaaaaaa ggagaacaca tgcacactaa aaagataaaa ctgtagagtc
     2761 ctgttgtcaa aatactcaat tgtcctttag accatgtcta actgttcatt tatatgattc
     2821 tctaaaacac tgatattatt gtagtactat agattatatt attcgtagag taaagtttaa
     2881 atatatgtat aaagatagat aaactgcact tcaaacaagt gtgacaaaaa aaatatgtgg
     2941 taatttttta taacttagac atgcaatgct cattatctct agagaggggc acgaccgggt
     3001 cacgctgcac tgcaggaatt cgatatcaag cttggcgtaa tcatggtcat agctgtttcc
     3061 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     3121 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     3181 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     3241 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     3301 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     3361 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     3421 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     3481 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     3541 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     3601 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     3661 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     3721 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     3781 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     3841 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     3901 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     3961 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     4021 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     4081 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     4141 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     4201 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     4261 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     4321 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     4381 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     4441 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     4501 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     4561 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     4621 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     4681 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     4741 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     4801 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     4861 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     4921 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     4981 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     5041 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     5101 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     5161 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     5221 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
     5281 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     5341 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     5401 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
     5461 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag
     5521 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     5581 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     5641 acgttgtaaa acgacggcca gt
//
