LOCUS       40924_47353        5659 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Transient expression vector pXUN-FLAG, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5659)
  AUTHORS   Chen S, Songkumarn P, Liu J, Wang GL.
  TITLE     A versatile zero background T-vector system for gene cloning and 
            functional genomics
  JOURNAL   Plant Physiol. 150 (3), 1111-1121 (2009)
  PUBMED    19403729
REFERENCE   2  (bases 1 to 5659)
  AUTHORS   Chen S, Songkumarn P, Liu J, Wang G-L.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2009) Department of Plant Pathology, The Ohio 
            State University, 201 Kottman Hall, 2021 Coffey Rd, Columbus, OH 
            43210, USA
REFERENCE   3  (bases 1 to 5659)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5659)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2009"; volume: "150"; issue: "3"; pages: 
            "1111-1121"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-APR-2009) Department of Plant Pathology, The Ohio State 
            University, 201 Kottman Hall, 2021 Coffey Rd, Columbus, OH 43210, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5659
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(9..261)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             622..924
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(983..1006)
                     /codon_start=1
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /translation="DYKDDDDK"
     promoter        complement(1022..3012)
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     primer_bind     complement(3043..3059)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3067..3083)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3091..3121)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3136..3157)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3445..4033)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4207..5064)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5065..5169)
                     /label=AmpR promoter
     primer_bind     5643..5659
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattcccga tctagtaaca tagatgacac cgcgcgcgat aatttatcct agtttgcgcg
       61 ctatattttg ttttctatcg cgtattaaat gtataattgc gggactctaa tcataaaaac
      121 ccatctcata aataacgtca tgcattacat gttaattatt acatgcttaa cgtaattcaa
      181 cagaaattat atgataatca tcgcaagacc ggcaacagga ttcaatctta agaaacttta
      241 ttgccaaatg tttgaacgat cggggaaatt cggatcccca atacttgtat ggggcttact
      301 aaaagccaga taacagtatg cgtatttgcg cgctgatttt tgcggtataa gaatatatac
      361 tgatatgtat acccgaagta tgtcaaaaag aggtatgcta tgaagcagcg tattacagtg
      421 acagttgaca gcgacagcta tcagttgctc aaggcatata tgatgtcaat atctccggtc
      481 tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg
      541 aaaatcagga agggatggct gaggtcgccc ggtttattga aatgaacggc tcttttgctg
      601 acgagaacag gggctggtga aatgcagttt aaggtttaca cctataaaag agagagccgt
      661 tatcgtctgt ttgtggatgt acagagtgat attattgaca cgcccgggcg acggatggtg
      721 atccccctgg ccagtgcacg tctgctgtca gataaagtct cccgtgaact ttacccggtg
      781 gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtc
      841 tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc
      901 attaacctga tgttctgggg aatataaatg tcaggctccc ttatacacag ccagtctgca
      961 ggtcgaccca tacaagtatt ggcttatcat catcatcctt gtaatccatg gatcccccgg
     1021 gctgcagaag taacaccaaa caacagggtg agcatcgaca aaagaaacag taccaagcaa
     1081 ataaatagcg tatgaaggca gggctaaaaa aatccacata tagctgctgc atatgccatc
     1141 atccaagtat atcaagatca aaataattat aaaacatact tgtttattat aatagatagg
     1201 tactcaaggt tagagcatat gaatagatgc tgcatatgcc atcatgtata tgcatcagta
     1261 aaacccacat caacatgtat acctatccta gatcgatatt tccatccatc ttaaactcgt
     1321 aactatgaag atgtatgaca cacacataca gttccaaaat taataaatac accaggtagt
     1381 ttgaaacagt attctactcc gatctagaac gaatgaacga ccgcccaacc acaccacatc
     1441 atcacaacca agcgaacaaa aagcatctct gtatatgcat cagtaaaacc cgcatcaaca
     1501 tgtataccta tcctagatcg atatttccat ccatcatctt caattcgtaa ctatgaatat
     1561 gtatggcaca cacatacaga tccaaaatta ataaatccac caggtagttt gaaacagaat
     1621 tctactccga tctagaacga ccgcccaacc agaccacatc atcacaacca agacaaaaaa
     1681 aagcatgaaa agatgacccg acaaacaagt gcacggcata tattgaaata aaggaaaagg
     1741 gcaaaccaaa ccctatgcaa cgaaacaaaa aaaatcatga aatcgatccc gtctgcggaa
     1801 cggctagagc catcccagga ttccccaaag agaaacactg gcaagttagc aatcagaacg
     1861 tgtctgacgt acaggtcgca tccgtgtacg aacgctagca gcacggatct aacacaaaca
     1921 cggatctaac acaaacatga acagaagtag aactaccggg ccctaaccat ggaccggaac
     1981 gccgatctag agaaggtaga gagggggggg gggggaggac gagcggcgta ccttgaagcg
     2041 gaggtgccga cgggtggatt tgggggagat ctggttgtgt gtgtgtgcgc tccgaacaac
     2101 acgaggttgg ggaaagaggg tgtggagggg gtgtctattt attacggcgg gcgaggaagg
     2161 gaaagcgaag gagcggtggg aaaggaatcc cccgtagctg ccgtgccgtg agaggaggag
     2221 gaggccgcct gccgtgccgg ctcacgtctg ccgctccgcc acgcatttct ggatgccgac
     2281 agcggagcaa gtccaacggt ggagcggaac tctcgagagg ggtccagagg cagcgacaga
     2341 gatgccgtgc cgtctgcttc gcttggcccg acgcgacgct gctggttcgc tggttggtgt
     2401 ccgttagact cgtcgacggc gtttaacagg ctggcattat ctactcgaaa caagaaaaat
     2461 gtttccttag tttttttaat ttcttaaagg gtatttgttt aatttttagt cactttattt
     2521 tattctattt tatatctaaa ttattaaata aaaaaactaa aatagagttt tagttttctt
     2581 aatttagagg ctaaaataga ataaaataga tgtactaaaa aaattagtct ataaaaacca
     2641 ttaaccctaa accctaaatg gatgtactaa taaaatggat gaagtattat ataggtgaag
     2701 ctatttgcaa aaaaaaagga gaacacatgc acactaaaaa gataaaactg tagagtcctg
     2761 ttgtcaaaat actcaattgt cctttagacc atgtctaact gttcatttat atgattctct
     2821 aaaacactga tattattgta gtactataga ttatattatt cgtagagtaa agtttaaata
     2881 tatgtataaa gatagataaa ctgcacttca aacaagtgtg acaaaaaaaa tatgtggtaa
     2941 ttttttataa cttagacatg caatgctcat tatctctaga gaggggcacg accgggtcac
     3001 gctgcactgc aggaattcga tatcaagctt ggcgtaatca tggtcatagc tgtttcctgt
     3061 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     3121 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     3181 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     3241 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     3301 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     3361 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     3421 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     3481 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     3541 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     3601 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     3661 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     3721 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     3781 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     3841 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     3901 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     3961 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     4021 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     4081 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     4141 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     4201 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     4261 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     4321 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     4381 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     4441 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     4501 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     4561 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     4621 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     4681 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     4741 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     4801 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     4861 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     4921 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     4981 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     5041 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     5101 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     5161 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     5221 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
     5281 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     5341 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     5401 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
     5461 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct
     5521 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     5581 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     5641 ttgtaaaacg acggccagt
//
