LOCUS       40924_47343        4855 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pXT99A, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Kirchner O, Tauch A.
  TITLE     Tools for genetic engineering in the amino acid-producing bacterium 
            Corynebacterium glutamicum
  JOURNAL   J. Biotechnol. 104 (1-3), 287-299 (2003)
  PUBMED    12948646
REFERENCE   2  (bases 1 to 4855)
  AUTHORS   Kirchner O, Tauch A.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JAN-2003) Department of Genetics, University of 
            Bielefeld, Universitaetsstrasse 25, Bielefeld D-33615, Germany
REFERENCE   3  (bases 1 to 4855)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4855)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Biotechnol. 104 (1-3), 287-299 (2003)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-JAN-2003) Department of Genetics, University of Bielefeld, 
            Universitaetsstrasse 25, Bielefeld D-33615, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4855
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..57
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     terminator      260..346
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      438..465
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     CDS             complement(997..1008)
                     /codon_start=1
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
     rep_origin      2287..2875
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(3061..3201)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     promoter        3387..3464
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             3465..4544
                     /codon_start=1
                     /label=lacI
                     /note="lac repressor"
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        4779..4808
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    4816..4832
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 gaattcgagc tcggtacccg gggatcctct agagtcgacc tgcaggcatg caagcttggc
       61 tgttttggcg gatgagagaa gattttcagc ctgatacaga ttaaatcaga acgcagaagc
      121 ggtctgataa aacagaattt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg
      181 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggtctcc ccatgcgaga
      241 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
      301 ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgggagcgga
      361 tttgaacgtt gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc cataaactgc
      421 caggcatcaa attaagcaga aggccatcct gacggatggc ctttttgcgt ttctacaaac
      481 tctttttgtt tatttttcta aatacattca aatatgtatc cgctcatgct ccttcgtcgg
      541 tgtcgtcgcc ggatggtctg cggtggtgct cagcgtggag acgcgcaccg tcacggaccg
      601 cgtgtagtgc gtggcggaaa cttcttgcgt ttcgcaagag aaatgcgtcc catttctcgt
      661 cggactcggg gaaggaagcg tgatgctctc ggtcaagcac gtcgctcgcc agcgctgcga
      721 ggagttcggc cttcgtgcgg aagtgccagt agaggccggg ctgctgtacc tgtaagtgag
      781 ccgccagcgc gcgagtggtg aagccatcga gcccagtctc gtcgagcacc tgccgggccc
      841 cgagcaacac ggacgtgcgg tcgagacgct tccggtggtg agtcatagtt gcactttatc
      901 atcgataact ttatcttaga taaagtgact gctcgctact ctcatctgac tgctcgctac
      961 tctcatcgtg gaatcctgac agccgtgctc atcacggcga ccctcgatgc tgcagggctg
     1021 ggcctcgtga tgccgatctt gcctaccctt ctcgaccagg tcggtgcccc cgacgacatg
     1081 atcccactgc acgtcggact actgacagcg ctctatgcga tcatgcagtt tctttgcgcc
     1141 ccgatccttg gccgactctc tgaccgtttc ggacgccgcc gcgtgcttgt cgcctccctc
     1201 gcaggcgcga cgatcgacta cctcgtgctc gcactgacgg acacgctgtg ggtcttttac
     1261 ctcgcccgcg cggttgcagg cattaccggc gccacgaacg ccgtcaccgc gacggtgatc
     1321 gccgacatta ctccgccgga tcagcgcgca aaacgctacg ggtggctcgg cgcatgctac
     1381 ggcggtggca tgatcgcggg tcccgccatt ggcggtcttt tcggcggggt ctcaccgcat
     1441 ctgccattcc tcgtcgccgc cgcgctcgcc ggaatcaccc tcgtactcag cgcgagtctt
     1501 ctgcgtgaga cgcggccacc gggcagcaac ggctcgcacg cacagcaacc cggtacggcg
     1561 aagcgaaccg cagtgccggg gatgcttatc cttctcgcag tcttcggcat cgtgcagttc
     1621 atcggccaag caccaggctc cacctgggtg ctcttcacgc agcagcgcct cgactggaac
     1681 cccgtcgaag tcggcgtttc gctatccatc ttcggaatgg tgcaagtatt cgtgcaggcg
     1741 gcactgaccg gacgcatcgt gtcccggatc ggcgagaccc gggcgatcct cgtcggtatc
     1801 gccgcagacg ccattgggct catcggcctt gccctcatcg ccagcacatg ggcgatgcta
     1861 ccgatcctcg cagcgctcgg actcggcagc atcacgttgc ccgcactgca gacgctgctc
     1921 tcgagacgcg cgcccgagca gcagcaggga cgcctgcagg gaacacttgc aagcctgaac
     1981 agcctcacct cgatcatcgg cccggtcacc ttcaccggca ttttcgcact cacccgaacg
     2041 aatgcagacg gcaccctctg gatctgcgcc gcagcgctct acgttctctg cgccctcctg
     2101 atgatccgtg agacatgcgc ctcacggcga tctcgataac cgcgctaagg tgccatcccg
     2161 atgcgacggg atcgctctgc caccagtcaa gtctcccgta gccggtatga gcatgaccaa
     2221 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     2281 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     2341 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     2401 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     2461 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     2521 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     2581 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     2641 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     2701 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     2761 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     2821 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     2881 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     2941 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     3001 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     3061 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac
     3121 tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta
     3181 cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     3241 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     3301 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agcagatcaa ttcgcgcgcg
     3361 aaggcgaagc ggcatgcatt tacgttgaca ccatcgaatg gtgcaaaacc tttcgcggta
     3421 tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt
     3481 tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc
     3541 aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga
     3601 attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg
     3661 ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc
     3721 gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag
     3781 cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact
     3841 atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt
     3901 tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg
     3961 gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag
     4021 cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca
     4081 ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt
     4141 ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca
     4201 acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg
     4261 cggatatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt
     4321 caaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc
     4381 aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa
     4441 gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     4501 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     4561 aatgtgagtt agcgcgaatt gatctggttt gacagcttat catcgactgc acggtgcacc
     4621 aatgcttctg gcgtcaggca gccatcggaa gctgtggtat ggctgtgcag gtcgtaaatc
     4681 actgcataat tcgtgtcgct caaggcgcac tcccgttctg gataatgttt tttgcgccga
     4741 catcataacg gttctggcaa atattctgaa atgagctgtt gacaattaat catccggctc
     4801 gtataatgtg tggaattgtg agcggataac aatttcacac aggaaacaga ccatg
//
