LOCUS       40924_47333        4565 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Transient expression vector pXSN-Myc, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4565)
  AUTHORS   Chen S, Songkumarn P, Liu J, Wang GL.
  TITLE     A versatile zero background T-vector system for gene cloning and 
            functional genomics
  JOURNAL   Plant Physiol. 150 (3), 1111-1121 (2009)
  PUBMED    19403729
REFERENCE   2  (bases 1 to 4565)
  AUTHORS   Chen S, Songkumarn P, Liu J, Wang G-L.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2009) Department of Plant Pathology, The Ohio 
            State University, 201 Kottman Hall, 2021 Coffey Rd, Columbus, OH 
            43210, USA
REFERENCE   3  (bases 1 to 4565)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4565)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2009"; volume: "150"; issue: "3"; pages: 
            "1111-1121"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-APR-2009) Department of Plant Pathology, The Ohio State 
            University, 201 Kottman Hall, 2021 Coffey Rd, Columbus, OH 43210, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4565
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(8..260)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             627..929
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(988..1017)
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
     promoter        complement(1170..1841)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     primer_bind     complement(1940..1956)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2430..2534
                     /label=AmpR promoter
     CDS             2535..3392
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3566..4154
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4442..4463
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4478..4508
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4516..4532
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4540..4556
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattccgat ctagtaacat agatgacacc gcgcgcgata atttatccta gtttgcgcgc
       61 tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc
      121 catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac gtaattcaac
      181 agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa gaaactttat
      241 tgccaaatgt ttgaacgatc ggggaaattc gctagtggat ccccaatact tgtatggggc
      301 ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg tataagaata
      361 tatactgata tgtatacccg aagtatgtca aaaagaggta tgctatgaag cagcgtatta
      421 cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg tcaatatctc
      481 cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa
      541 agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga acggctcttt
      601 tgctgacgag aacaggggct ggtgaaatgc agtttaaggt ttacacctat aaaagagaga
      661 gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc gggcgacgga
      721 tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc
      781 cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc
      841 cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa
      901 acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt
      961 ctgcaggtcg acccatacaa gtattggaag atcttcttca gaaatcaact tttgttccat
     1021 ggatccccct cgagtatcgt tcgtaaatgg tgaaaatttt cagaaaattg cttttgcttt
     1081 aaaagaaatg atttaaattg ctgcaataga agtagaatgc ttgattgctt gagattcgtt
     1141 tgttttgtat atgttgtgtt gaggtcgagg tcctctccaa atgaaatgaa cttccttata
     1201 tagaggaagg gtcttgcgaa ggatagtggg attgtgcgtc atcccttacg tcagtggaga
     1261 tatcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc
     1321 tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct tcaacgatgg
     1381 cctttccttt atcgcaatga tggcatttgt aggagccacc ttccttttcc actatcttca
     1441 caataaagtg acagatagct gggcaatgga atccgaggag gtttccggat attacccttt
     1501 gttgaaaagt ctcaattgcc ctttggtctt ctgagactgt atctttgata tttttggagt
     1561 agacaagtgt gtcgtgctcc accatgttat cacatcaatc cacttgcttt gaagacgtgg
     1621 ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg gggtccatct ttgggaccac
     1681 tgtcggcaga ggcatcttca acgatggcct ttcctttatc gcaatgatgg catttgtagg
     1741 agccaccttc cttttccact atcttcacaa taaagtgaca gatagctggg caatggaatc
     1801 cgaggaggtt tccggatatt accctttgtt gaaaagtctc aattgccctt tggtcttctg
     1861 agactgtatc tttgatattt ttggagtaga caagtgtgtc gtgctccacc atgttgacct
     1921 gcaggcatgc aagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     1981 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     2041 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc
     2101 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
     2161 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     2221 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     2281 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     2341 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     2401 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     2461 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     2521 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     2581 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     2641 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     2701 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     2761 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     2821 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     2881 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     2941 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     3001 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     3061 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     3121 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     3181 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     3241 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     3301 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     3361 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     3421 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     3481 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     3541 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     3601 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     3661 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     3721 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     3781 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     3841 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     3901 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     3961 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     4021 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     4081 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     4141 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     4201 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     4261 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     4321 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     4381 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     4441 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     4501 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     4561 attac
//
