LOCUS       40924_47328        4562 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Transient expression vector pXSN-HA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4562)
  AUTHORS   Chen S, Songkumarn P, Liu J, Wang GL.
  TITLE     A versatile zero background T-vector system for gene cloning and 
            functional genomics
  JOURNAL   Plant Physiol. 150 (3), 1111-1121 (2009)
  PUBMED    19403729
REFERENCE   2  (bases 1 to 4562)
  AUTHORS   Chen S, Songkumarn P, Liu J, Wang G-L.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2009) Department of Plant Pathology, The Ohio 
            State University, 201 Kottman Hall, 2021 Coffey Rd, Columbus, OH 
            43210, USA
REFERENCE   3  (bases 1 to 4562)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4562)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2009"; volume: "150"; issue: "3"; pages: 
            "1111-1121"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-APR-2009) Department of Plant Pathology, The Ohio State 
            University, 201 Kottman Hall, 2021 Coffey Rd, Columbus, OH 43210, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4562
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(8..260)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             627..929
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(988..1014)
                     /codon_start=1
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
     promoter        complement(1167..1838)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     primer_bind     complement(1937..1953)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2427..2531
                     /label=AmpR promoter
     CDS             2532..3389
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3563..4151
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4439..4460
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4475..4505
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4513..4529
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4537..4553
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattccgat ctagtaacat agatgacacc gcgcgcgata atttatccta gtttgcgcgc
       61 tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc
      121 catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac gtaattcaac
      181 agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa gaaactttat
      241 tgccaaatgt ttgaacgatc ggggaaattc gctagtggat ccccaatact tgtatggggc
      301 ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg tataagaata
      361 tatactgata tgtatacccg aagtatgtca aaaagaggta tgctatgaag cagcgtatta
      421 cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg tcaatatctc
      481 cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa
      541 agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga acggctcttt
      601 tgctgacgag aacaggggct ggtgaaatgc agtttaaggt ttacacctat aaaagagaga
      661 gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc gggcgacgga
      721 tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc
      781 cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc
      841 cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa
      901 acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt
      961 ctgcaggtcg acccatacaa gtattggagc gtaatctgga acatcgtatg ggtacatgga
     1021 tccccctcga gtatcgttcg taaatggtga aaattttcag aaaattgctt ttgctttaaa
     1081 agaaatgatt taaattgctg caatagaagt agaatgcttg attgcttgag attcgtttgt
     1141 tttgtatatg ttgtgttgag gtcgaggtcc tctccaaatg aaatgaactt ccttatatag
     1201 aggaagggtc ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat
     1261 cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc
     1321 tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttca acgatggcct
     1381 ttcctttatc gcaatgatgg catttgtagg agccaccttc cttttccact atcttcacaa
     1441 taaagtgaca gatagctggg caatggaatc cgaggaggtt tccggatatt accctttgtt
     1501 gaaaagtctc aattgccctt tggtcttctg agactgtatc tttgatattt ttggagtaga
     1561 caagtgtgtc gtgctccacc atgttatcac atcaatccac ttgctttgaa gacgtggttg
     1621 gaacgtcttc tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt
     1681 cggcagaggc atcttcaacg atggcctttc ctttatcgca atgatggcat ttgtaggagc
     1741 caccttcctt ttccactatc ttcacaataa agtgacagat agctgggcaa tggaatccga
     1801 ggaggtttcc ggatattacc ctttgttgaa aagtctcaat tgccctttgg tcttctgaga
     1861 ctgtatcttt gatatttttg gagtagacaa gtgtgtcgtg ctccaccatg ttgacctgca
     1921 ggcatgcaag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     1981 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     2041 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga
     2101 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
     2161 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     2221 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     2281 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     2341 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     2401 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     2461 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     2521 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     2581 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     2641 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     2701 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     2761 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     2821 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     2881 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     2941 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     3001 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     3061 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     3121 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     3181 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     3241 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     3301 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     3361 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     3421 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     3481 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     3541 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     3601 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     3661 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     3721 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     3781 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     3841 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     3901 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     3961 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     4021 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     4081 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     4141 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     4201 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     4261 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     4321 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     4381 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     4441 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     4501 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     4561 ac
//
