LOCUS       40924_47323        4559 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Transient expression vector pXSN-FLAG, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Chen S, Songkumarn P, Liu J, Wang GL.
  TITLE     A versatile zero background T-vector system for gene cloning and 
            functional genomics
  JOURNAL   Plant Physiol. 150 (3), 1111-1121 (2009)
  PUBMED    19403729
REFERENCE   2  (bases 1 to 4559)
  AUTHORS   Chen S, Songkumarn P, Liu J, Wang G-L.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2009) Department of Plant Pathology, The Ohio 
            State University, 201 Kottman Hall, 2021 Coffey Rd, Columbus, OH 
            43210, USA
REFERENCE   3  (bases 1 to 4559)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4559)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2009"; volume: "150"; issue: "3"; pages: 
            "1111-1121"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-APR-2009) Department of Plant Pathology, The Ohio State 
            University, 201 Kottman Hall, 2021 Coffey Rd, Columbus, OH 43210, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4559
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(8..260)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             627..929
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(988..1011)
                     /codon_start=1
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /translation="DYKDDDDK"
     promoter        complement(1164..1835)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     primer_bind     complement(1934..1950)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2424..2528
                     /label=AmpR promoter
     CDS             2529..3386
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3560..4148
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4436..4457
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4472..4502
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4510..4526
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4534..4550
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattccgat ctagtaacat agatgacacc gcgcgcgata atttatccta gtttgcgcgc
       61 tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc
      121 catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac gtaattcaac
      181 agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa gaaactttat
      241 tgccaaatgt ttgaacgatc ggggaaattc gctagtggat ccccaatact tgtatggggc
      301 ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg tataagaata
      361 tatactgata tgtatacccg aagtatgtca aaaagaggta tgctatgaag cagcgtatta
      421 cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg tcaatatctc
      481 cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa
      541 agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga acggctcttt
      601 tgctgacgag aacaggggct ggtgaaatgc agtttaaggt ttacacctat aaaagagaga
      661 gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc gggcgacgga
      721 tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc
      781 cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc
      841 cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa
      901 acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt
      961 ctgcaggtcg acccatacaa gtattggctt atcatcatca tccttgtaat ccatggatcc
     1021 ccctcgagta tcgttcgtaa atggtgaaaa ttttcagaaa attgcttttg ctttaaaaga
     1081 aatgatttaa attgctgcaa tagaagtaga atgcttgatt gcttgagatt cgtttgtttt
     1141 gtatatgttg tgttgaggtc gaggtcctct ccaaatgaaa tgaacttcct tatatagagg
     1201 aagggtcttg cgaaggatag tgggattgtg cgtcatccct tacgtcagtg gagatatcac
     1261 atcaatccac ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg
     1321 tgggtggggg tccatctttg ggaccactgt cggcagaggc atcttcaacg atggcctttc
     1381 ctttatcgca atgatggcat ttgtaggagc caccttcctt ttccactatc ttcacaataa
     1441 agtgacagat agctgggcaa tggaatccga ggaggtttcc ggatattacc ctttgttgaa
     1501 aagtctcaat tgccctttgg tcttctgaga ctgtatcttt gatatttttg gagtagacaa
     1561 gtgtgtcgtg ctccaccatg ttatcacatc aatccacttg ctttgaagac gtggttggaa
     1621 cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg
     1681 cagaggcatc ttcaacgatg gcctttcctt tatcgcaatg atggcatttg taggagccac
     1741 cttccttttc cactatcttc acaataaagt gacagatagc tgggcaatgg aatccgagga
     1801 ggtttccgga tattaccctt tgttgaaaag tctcaattgc cctttggtct tctgagactg
     1861 tatctttgat atttttggag tagacaagtg tgtcgtgctc caccatgttg acctgcaggc
     1921 atgcaagctt ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     1981 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     2041 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc
     2101 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
     2161 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     2221 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     2281 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     2341 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     2401 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     2461 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     2521 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     2581 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     2641 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     2701 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     2761 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     2821 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     2881 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     2941 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     3001 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     3061 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     3121 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     3181 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     3241 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     3301 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     3361 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     3421 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     3481 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     3541 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     3601 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     3661 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     3721 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     3781 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     3841 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     3901 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     3961 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     4021 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     4081 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     4141 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     4201 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     4261 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     4321 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     4381 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     4441 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     4501 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattac
//
