LOCUS       40924_47313        5267 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Plant binary vector pXS2pat-Strep, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5267)
  AUTHORS   Cao FQ, Werner AK, Dahncke K, Romeis T, Liu LH, Witte CP.
  TITLE     Identification and characterization of proteins involved in rice 
            urea and arginine catabolism
  JOURNAL   Plant Physiol. 154 (1), 98-108 (2010)
  PUBMED    20631318
REFERENCE   2  (bases 1 to 5267)
  AUTHORS   Cao FQ, Dahncke K, Liu LH, Romeis T, Werner AK, Witte C-P.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAY-2010) Dahlem Centre of Plant Science, Department 
            of Plant Biochemistry, Freie Universitaet Berlin, 
            Koenigin-Luise-Str. 12-16, Berlin 14195, Germany
REFERENCE   3  (bases 1 to 5267)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5267)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2010"; volume: "154"; issue: "1"; pages: "98-108"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-MAY-2010) Dahlem Centre of Plant Science, Department of Plant 
            Biochemistry, Freie Universitaet Berlin, Koenigin-Luise-Str. 12-16, 
            Berlin 14195, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5267
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(45..61)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    344..368
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      480..1189
                     /label=oriV
                     /note="incP origin of replication"
     promoter        1263..1354
                     /label=AmpR promoter
     CDS             1355..2212
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2386..2974
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    3286..3310
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(3404..3656)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3679..4227)
                     /codon_start=1
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(4270..4449)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        4593..5266
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
ORIGIN
        1 tcgagaaaag cgatataaaa ccatggaatt cctgcagccc gggggatcca ctagttctag
       61 agtccgcaaa aatcaccagt ctctctctac aaatctatct ctctctattt ttctccagaa
      121 taatgtgtga gtagttccca gataagggaa ttagggttct tatagggttt cgctcatgtg
      181 ttgagcatat aagaaaccct tagtatgtat ttgtatttgt aaaatacttc tatcaataaa
      241 atttctaatt cctaaaacca aaatccagtg accgggcggc cgccaccgcg gtggaggggg
      301 atcagattgt cgtttcccgc cttcagttta aactatcagt gtttgacagg atatattggc
      361 gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa agggcgtgaa
      421 aaggtttatc cgttcgtcca tttgtatgtg tacatcaccg acgagcaagg caagaccgag
      481 cgcctttccg acgctcaccg ggctggttgc cctcgccgct gggctggcgg ccgtctatgg
      541 ccctgcaaac gcgccagaaa cgccgtcgaa gccgtgtgcg agacaccggc cgccggcgtt
      601 gtggatacct cgcggaaaac ttggccctca ctgacagatg aggggcggac gttgacactt
      661 gaggggccga ctcacccggc gcggcgttga cagatgaggg gcaggctcga tttcggccgg
      721 cgacgtggag ctggccagcc tcgcaaatcg gcgaaaacgc ctgattttac gcgagtttcc
      781 cacagatgat gtggacaagc ctggggataa gtgccctgcg gtattgacac ttgaggggcg
      841 cgactactga cagatgaggg gcgcgatcct tgacacttga ggggcagagt gctgacagat
      901 gaggggcgca cctattgaca tttgaggggc tgtccacagg cagaaaatcc agcatttgca
      961 agggtttccg cccgtttttc ggccaccgct aacctgtctt ttaacctgct tttaaaccaa
     1021 tatttataaa ccttgttttt aaccagggct gcgccctgtg cgcgtgaccg cgcacgccga
     1081 aggggggtgc ccccccttct cgaaccctcc cggcccgcta acgcgggcct cccatccccc
     1141 caggggctgc gcccctcggc cgcgaacggc ctcaccccaa aaatggcagc gctggcagtc
     1201 cttgccattg ccgggatcgg ggcagtaacg ggatgggcga tcagcccgac aagctacccc
     1261 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     1321 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     1381 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     1441 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     1501 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     1561 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     1621 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     1681 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     1741 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     1801 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     1861 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     1921 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     1981 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     2041 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     2101 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     2161 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     2221 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     2281 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     2341 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     2401 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     2461 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     2521 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     2581 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     2641 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     2701 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     2761 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     2821 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     2881 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     2941 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     3001 gttcctggcc ttttgctggc cttttgctca catggactct agctagagga tcacaggcag
     3061 caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa
     3121 acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca aagtctgccg
     3181 ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg tatcgagtgg
     3241 tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca ggatatattg
     3301 tggtgtaaac aaattgacgc ttagacaact taataacaca ttgcggacgt ttttaatgat
     3361 cgaatactaa cgtctctacc agatatcagc ttgcatgccg gtcgatctag taacatagat
     3421 gacaccgcgc gcgataattt atcctagttt gcgcgctata ttttgttttc tatcgcgtat
     3481 taaatgtata attgcgggac tctaatcata aaaacccatc tcataaataa cgtcatgcat
     3541 tacatgttaa ttattacatg cttaacgtaa ttcaacagaa attatatgat aatcatcgca
     3601 agaccggcaa caggattcaa tcttaagaaa ctttattgcc aaatgtttga acgatctgct
     3661 tgactctagg ggtcatcaga tttcggtgac gggcaggacc ggacggggcg gcaccggcag
     3721 gctgaagtcc agctgccaga aacccacgtc atgccagttc ccgtgcttga agccggccgc
     3781 ccgcagcatg ccgcgggggg catatccgag cgcctcgtgc atgcgcacgc tcgggtcgtt
     3841 gggcagcccg atgacagcga ccacgctctt gaagccctgt gcctccaggg acttcagcag
     3901 gtgggtgtag agcgtggagc ccagtcccgt ccgctggtgg cggggggaga cgtacacggt
     3961 cgactcggcc gtccagtcgt aggcgttgcg tgccttccag gggcccgcgt aggcgatgcc
     4021 ggcgacctcg ccgtccacct cggcgacgag ccagggatag cgctcccgca gacggacgag
     4081 gtcgtccgtc cactcctgcg gttcctgcgg ctcggtacgg aagttgaccg tgcttgtctc
     4141 gatgtagtgg ttgacgatgg tgcagaccgc cggcatgtcc gcctcggtgg cacggcggat
     4201 gtcggccggg cgtcgttctg ggctcatggt agatccccct cgatcgagtt gagagtgaat
     4261 atgagactct aattggatac cgaggggaat ttatggaacg tcagtggagc atttttgaca
     4321 agaaatattt gctagctgat agtgacctta ggcgactttt gaacgcgcaa taatggtttc
     4381 tgacgtatgt gcttagctca ttaaactcca gaaacccgcg gctgagtggc tccttcaacg
     4441 ttgcggttct gtcagttcca aacgtaaaac ggcttgtccc gcgtcatcgg cgggggtcat
     4501 aacgtgactc ccttaattct ccgctcatga tcagattgtc gtttcccgcc ttcggtttgg
     4561 gcgcgcccgg tctcagaaga ccagagggct attgagactt ttcaacaaag ggtaatatcg
     4621 ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcgaaag gacagtagaa
     4681 aaggaagatg gcttctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagaa
     4741 tgcctctacc gacagtggtc ccaaagatgg acccccaccc acgaggaaca tcgtggaaaa
     4801 agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgataaca tggtggagca
     4861 cgacactctc gtctactcca agaatatcaa agatacagtc tcagaagacc agagggctat
     4921 tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat
     4981 ctgtcacttc atcgaaagga cagtagaaaa ggaagatggc ttctacaaat gccatcattg
     5041 cgataaagga aaggctatcg ttcaagaatg cctctaccga cagtggtccc aaagatggac
     5101 ccccacccac gaggaacatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag
     5161 tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc
     5221 aagacccttc ctctatataa ggaagttcat ttcatttgga gaggacc
//
