LOCUS       40924_47308        5268 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Plant binary vector pXS1pat-Strep, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5268)
  AUTHORS   Cao FQ, Werner AK, Dahncke K, Romeis T, Liu LH, Witte CP.
  TITLE     Identification and characterization of proteins involved in rice 
            urea and arginine catabolism
  JOURNAL   Plant Physiol. 154 (1), 98-108 (2010)
  PUBMED    20631318
REFERENCE   2  (bases 1 to 5268)
  AUTHORS   Cao FQ, Dahncke K, Liu LH, Romeis T, Werner AK, Witte C-P.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAY-2010) Dahlem Centre of Plant Science, Department 
            of Plant Biochemistry, Freie Universitaet Berlin, 
            Koenigin-Luise-Str. 12-16, Berlin 14195, Germany
REFERENCE   3  (bases 1 to 5268)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5268)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2010"; volume: "154"; issue: "1"; pages: "98-108"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-MAY-2010) Dahlem Centre of Plant Science, Department of Plant 
            Biochemistry, Freie Universitaet Berlin, Koenigin-Luise-Str. 12-16, 
            Berlin 14195, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5268
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(46..62)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    345..369
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      481..1190
                     /label=oriV
                     /note="incP origin of replication"
     promoter        1264..1355
                     /label=AmpR promoter
     CDS             1356..2213
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2387..2975
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    3287..3311
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(3405..3657)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3680..4228)
                     /codon_start=1
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(4271..4450)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        4594..5267
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
ORIGIN
        1 tcgagaaaag cgatataaaa catatggaat tcctgcagcc cgggggatcc actagttcta
       61 gagtccgcaa aaatcaccag tctctctcta caaatctatc tctctctatt tttctccaga
      121 ataatgtgtg agtagttccc agataaggga attagggttc ttatagggtt tcgctcatgt
      181 gttgagcata taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa
      241 aatttctaat tcctaaaacc aaaatccagt gaccgggcgg ccgccaccgc ggtggagggg
      301 gatcagattg tcgtttcccg ccttcagttt aaactatcag tgtttgacag gatatattgg
      361 cgggtaaacc taagagaaaa gagcgtttat tagaataatc ggatatttaa aagggcgtga
      421 aaaggtttat ccgttcgtcc atttgtatgt gtacatcacc gacgagcaag gcaagaccga
      481 gcgcctttcc gacgctcacc gggctggttg ccctcgccgc tgggctggcg gccgtctatg
      541 gccctgcaaa cgcgccagaa acgccgtcga agccgtgtgc gagacaccgg ccgccggcgt
      601 tgtggatacc tcgcggaaaa cttggccctc actgacagat gaggggcgga cgttgacact
      661 tgaggggccg actcacccgg cgcggcgttg acagatgagg ggcaggctcg atttcggccg
      721 gcgacgtgga gctggccagc ctcgcaaatc ggcgaaaacg cctgatttta cgcgagtttc
      781 ccacagatga tgtggacaag cctggggata agtgccctgc ggtattgaca cttgaggggc
      841 gcgactactg acagatgagg ggcgcgatcc ttgacacttg aggggcagag tgctgacaga
      901 tgaggggcgc acctattgac atttgagggg ctgtccacag gcagaaaatc cagcatttgc
      961 aagggtttcc gcccgttttt cggccaccgc taacctgtct tttaacctgc ttttaaacca
     1021 atatttataa accttgtttt taaccagggc tgcgccctgt gcgcgtgacc gcgcacgccg
     1081 aaggggggtg cccccccttc tcgaaccctc ccggcccgct aacgcgggcc tcccatcccc
     1141 ccaggggctg cgcccctcgg ccgcgaacgg cctcacccca aaaatggcag cgctggcagt
     1201 ccttgccatt gccgggatcg gggcagtaac gggatgggcg atcagcccga caagctaccc
     1261 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     1321 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     1381 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     1441 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     1501 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     1561 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     1621 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     1681 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     1741 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     1801 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     1861 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     1921 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     1981 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     2041 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     2101 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     2161 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     2221 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     2281 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     2341 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     2401 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     2461 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     2521 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     2581 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     2641 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     2701 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     2761 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     2821 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     2881 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     2941 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     3001 ggttcctggc cttttgctgg ccttttgctc acatggactc tagctagagg atcacaggca
     3061 gcaacgctct gtcatcgtta caatcaacat gctaccctcc gcgagatcat ccgtgtttca
     3121 aacccggcag cttagttgcc gttcttccga atagcatcgg taacatgagc aaagtctgcc
     3181 gccttacaac ggctctcccg ctgacgccgt cccggactga tgggctgcct gtatcgagtg
     3241 gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt
     3301 gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg tttttaatga
     3361 tcgaatacta acgtctctac cagatatcag cttgcatgcc ggtcgatcta gtaacataga
     3421 tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat attttgtttt ctatcgcgta
     3481 ttaaatgtat aattgcggga ctctaatcat aaaaacccat ctcataaata acgtcatgca
     3541 ttacatgtta attattacat gcttaacgta attcaacaga aattatatga taatcatcgc
     3601 aagaccggca acaggattca atcttaagaa actttattgc caaatgtttg aacgatctgc
     3661 ttgactctag gggtcatcag atttcggtga cgggcaggac cggacggggc ggcaccggca
     3721 ggctgaagtc cagctgccag aaacccacgt catgccagtt cccgtgcttg aagccggccg
     3781 cccgcagcat gccgcggggg gcatatccga gcgcctcgtg catgcgcacg ctcgggtcgt
     3841 tgggcagccc gatgacagcg accacgctct tgaagccctg tgcctccagg gacttcagca
     3901 ggtgggtgta gagcgtggag cccagtcccg tccgctggtg gcggggggag acgtacacgg
     3961 tcgactcggc cgtccagtcg taggcgttgc gtgccttcca ggggcccgcg taggcgatgc
     4021 cggcgacctc gccgtccacc tcggcgacga gccagggata gcgctcccgc agacggacga
     4081 ggtcgtccgt ccactcctgc ggttcctgcg gctcggtacg gaagttgacc gtgcttgtct
     4141 cgatgtagtg gttgacgatg gtgcagaccg ccggcatgtc cgcctcggtg gcacggcgga
     4201 tgtcggccgg gcgtcgttct gggctcatgg tagatccccc tcgatcgagt tgagagtgaa
     4261 tatgagactc taattggata ccgaggggaa tttatggaac gtcagtggag catttttgac
     4321 aagaaatatt tgctagctga tagtgacctt aggcgacttt tgaacgcgca ataatggttt
     4381 ctgacgtatg tgcttagctc attaaactcc agaaacccgc ggctgagtgg ctccttcaac
     4441 gttgcggttc tgtcagttcc aaacgtaaaa cggcttgtcc cgcgtcatcg gcgggggtca
     4501 taacgtgact cccttaattc tccgctcatg atcagattgt cgtttcccgc cttcggtttg
     4561 ggcgcgcccg gtctcagaag accagagggc tattgagact tttcaacaaa gggtaatatc
     4621 gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcgaaa ggacagtaga
     4681 aaaggaagat ggcttctaca aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga
     4741 atgcctctac cgacagtggt cccaaagatg gacccccacc cacgaggaac atcgtggaaa
     4801 aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgataac atggtggagc
     4861 acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac cagagggcta
     4921 ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat tgcccagcta
     4981 tctgtcactt catcgaaagg acagtagaaa aggaagatgg cttctacaaa tgccatcatt
     5041 gcgataaagg aaaggctatc gttcaagaat gcctctaccg acagtggtcc caaagatgga
     5101 cccccaccca cgaggaacat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa
     5161 gtggattgat gtgatatctc cactgacgta agggatgacg cacaatccca ctatccttcg
     5221 caagaccctt cctctatata aggaagttca tttcatttgg agaggacc
//
