LOCUS       V014540                14416 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014540
VERSION     V014540
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 14416)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..14416
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        238..617
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        620..820
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             835..1015
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1680..1913
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             2098..2142
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             2291..2332
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2440..2557
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2589..2829
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_RNA        2839..2874
                     /label="RfxCas13d DR36"
                     /note="36-base direct repeat for an RfxCas13d pre-gRNA
                     (Konermann et al., 2018)"
     promoter        3024..4202
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     CDS             4221..4247
                     /label="NES"
                     /note="nuclear export signal from the HIV Rev protein
                     (Fischer et al., 1995)"
     CDS             7533..8228
                     /label="BioID2"
                     /note="promiscuous R40G mutant of a biotin protein ligase
                     from Aquifex aeolicus (Kim et al., 2016)"
     CDS             8235..8261
                     /label="NES"
                     /note="nuclear export signal from the HIV Rev protein
                     (Fischer et al., 1995)"
     CDS             8271..8297
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             8367..9080
                     /label="EGFP"
                     /note="enhanced GFP"
     misc_feature    9108..9696
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             10221..10401
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    10433..10657
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10703..11131
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        11145..11474
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        11522..11569
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             11588..11959
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    12092..12225
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(12310..12340)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(12355..12376)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(12664..13252)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(13426..14283)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(14284..14388)
                     /label="AmpR promoter"
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggta atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagga gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg
     2641 ttagagagat aattggaatt aatttgactg taaacacaaa gatattagta caaaatacgt
     2701 gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg
     2761 actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     2821 ggaaaggacg aaacaccgca agtaaacccc taccaactgg tcggggtttg aaacggagac
     2881 ggacgtctct caagtaaacc cctaccaact ggtcggggtt tgaaactttt tttggttaat
     2941 taatgcaaag atggataaag ttttaaacag agaggaatct tttcagctaa tggaccttct
     3001 aggtcttgaa aggagtggga attggctccg gtgcccgtca gtgggcagag cgcacatcgc
     3061 ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt
     3121 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg
     3181 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg
     3241 ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt
     3301 atggcccttg cgtgccttga attacttcca cctggctgca gtacgtgatt cttgatcccg
     3361 agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga gccccttcgc
     3421 ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga atctggtggc
     3481 accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgac
     3541 ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca
     3601 ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat
     3661 gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg tagtctcaag
     3721 ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg
     3781 caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt cccggccctg
     3841 ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt gagtcaccca
     3901 cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc
     3961 gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg tctttaggtt
     4021 ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta
     4081 ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt
     4141 ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt
     4201 gacgtacggc caccatgagc ctgcctccac ttgaaagact gacactggag gccagcatga
     4261 acatccccgc tctggtggaa aaccagaaga agtactttgg cacctacagc gtgatggcca
     4321 tgctgaacgc tcagaccgtg ctggaccaca tccagaaggt ggccgatatt gagggcgagc
     4381 agaacgagaa caacgagaat ctgtggtttc accccgtgat gagccacctg tacaacgcca
     4441 agaacggcta cgacaagcag cccgagaaaa ccatgttcat catcgagcgg ctgcagagct
     4501 acttcccatt cctgaagatc atggccgaga accagagaga gtacagcaac ggcaagtaca
     4561 agcagaaccg cgtggaagtg aacagcaacg acatcttcga ggtgctgaag cgcgccttcg
     4621 gcgtgctgaa gatgtacagg gacctgacca acgcatacaa gacctacgag gaaaagctga
     4681 acgacggctg cgagttcctg accagcacag agcaacctct gagcggcatg atcaacaact
     4741 actacacagt ggccctgcgg aacatgaacg agagatacgg ctacaagaca gaggacctgg
     4801 ccttcatcca ggacaagcgg ttcaagttcg tgaaggacgc ctacggcaag aaaaagtccc
     4861 aagtgaatac cggattcttc ctgagcctgc aggactacaa cggcgacaca cagaagaagc
     4921 tgcacctgag cggagtggga atcgccctgc tgatctgcct gttcctggac aagcagtaca
     4981 tcaacatctt tctgagcagg ctgcccatct tctccagcta caatgcccag agcgaggaac
     5041 ggcggatcat catcagatcc ttcggcatca acagcatcaa gctgcccaag gaccggatcc
     5101 acagcgagaa gtccaacaag agcgtggcca tggatatgct caacgaagtg aagcggtgcc
     5161 ccgacgagct gttcacaaca ctgtctgccg agaagcagtc ccggttcaga atcatcagcg
     5221 acgaccacaa tgaagtgctg atgaagcgga gcagcgacag attcgtgcct ctgctgctgc
     5281 agtatatcga ttacggcaag ctgttcgacc acatcaggtt ccacgtgaac atgggcaagc
     5341 tgagatacct gctgaaggcc gacaagacct gcatcgacgg ccagaccaga gtcagagtga
     5401 tcgagcagcc cctgaacggc ttcggcagac tggaagaggc cgagacaatg cggaagcaag
     5461 agaacggcac cttcggcaac agcggcatcc ggatcagaga cttcgagaac atgaagcggg
     5521 acgacgccaa tcctgccaac tatccctaca tcgtggacac ctacacacac tacatcctgg
     5581 aaaacaacaa ggtcgagatg tttatcaacg acaaagagga cagcgcccca ctgctgcccg
     5641 tgatcgagga tgatagatac gtggtcaaga caatccccag ctgccggatg agcaccctgg
     5701 aaattccagc catggccttc cacatgtttc tgttcggcag caagaaaacc gagaagctga
     5761 tcgtggacgt gcacaaccgg tacaagagac tgttccaggc catgcagaaa gaagaagtga
     5821 ccgccgagaa tatcgccagc ttcggaatcg ccgagagcga cctgcctcag aagatcctgg
     5881 atctgatcag cggcaatgcc cacggcaagg atgtggacgc cttcatcaga ctgaccgtgg
     5941 acgacatgct gaccgacacc gagcggagaa tcaagagatt caaggacgac cggaagtcca
     6001 ttcggagcgc cgacaacaag atgggaaaga gaggcttcaa gcagatctcc acaggcaagc
     6061 tggccgactt cctggccaag gacatcgtgc tgtttcagcc cagcgtgaac gatggcgaga
     6121 acaagatcac cggcctgaac taccggatca tgcagagcgc cattgccgtg tacgatagcg
     6181 gcgacgatta cgaggccaag cagcagttca agctgatgtt cgagaaggcc cggctgatcg
     6241 gcaagggcac aacagagcct catccatttc tgtacaaggt gttcgcccgc agcatccccg
     6301 ccaatgccgt cgagttctac gagcgctacc tgatcgagcg gaagttctac ctgaccggcc
     6361 tgtccaacga gatcaagaaa ggcaacagag tggatgtgcc cttcatccgg cgggaccaga
     6421 acaagtggaa aacacccgcc atgaagaccc tgggcagaat ctacagcgag gatctgcccg
     6481 tggaactgcc cagacagatg ttcgacaatg agatcaagtc ccacctgaag tccctgccac
     6541 agatggaagg catcgacttc aacaatgcca acgtgaccta tctgatcgcc gagtacatga
     6601 agagagtgct ggacgacgac ttccagacct tctaccagtg gaaccgcaac taccggtaca
     6661 tggacatgct taagggcgag tacgacagaa agggctccct gcagcactgc ttcaccagcg
     6721 tggaagagag agaaggcctc tggaaagagc gggcctccag aacagagcgg tacagaaagc
     6781 aggccagcaa caagatccgc agcaaccggc agatgagaaa cgccagcagc gaagagatcg
     6841 agacaatcct ggataagcgg ctgagcaaca gccggaacga gtaccagaaa agcgagaaag
     6901 tgatccggcg ctacagagtg caggatgccc tgctgtttct gctggccaaa aagaccctga
     6961 ccgaactggc cgatttcgac ggcgagaggt tcaaactgaa agaaatcatg cccgacgccg
     7021 agaagggaat cctgagcgag atcatgccca tgagcttcac cttcgagaaa ggcggcaaga
     7081 agtacaccat caccagcgag ggcatgaagc tgaagaacta cggcgacttc tttgtgctgg
     7141 ctagcgacaa gaggatcggc aacctgctgg aactcgtggg cagcgacatc gtgtccaaag
     7201 aggatatcat ggaagagttc aacaaatacg accagtgcag gcccgagatc agctccatcg
     7261 tgttcaacct ggaaaagtgg gccttcgaca cataccccga gctgtctgcc agagtggacc
     7321 gggaagagaa ggtggacttc aagagcatcc tgaaaatcct gctgaacaac aagaacatca
     7381 acaaagagca gagcgacatc ctgcggaaga tccggaacgc cttcgatgca aacaattacc
     7441 ccgacaaagg cgtggtggaa atcaaggccc tgcctgagat cgccatgagc atcaagaagg
     7501 cctttgggga gtacgccatc atgaagggat ccttcaagaa cctgatctgg ctgaaggagg
     7561 tggacagcac ccaggagaga ctgaaggagt ggaacgtgag ctacggcacc gccctggtgg
     7621 ccgacagaca gaccaagggc agaggcggcc tgggcagaaa gtggctgagc caggagggcg
     7681 gcctgtactt cagcttcctg ctgaacccca aggagttcga gaacctgctg cagctgcccc
     7741 tggtgctggg cctgagcgtg agcgaggccc tggaggagat caccgagatc cccttcagcc
     7801 tgaagtggcc caacgacgtg tacttccagg agaagaaggt gagcggcgtg ctgtgcgagc
     7861 tgagcaagga caagctgatc gtgggcatcg gcatcaacgt gaaccagaga gagatccccg
     7921 aggagatcaa ggacagagcc accaccctgt acgagatcac cggcaaggac tgggacagaa
     7981 aggaggtgct gctgaaggtg ctgaagagaa tcagcgagaa cctgaagaag ttcaaggaga
     8041 agagcttcaa ggagttcaag ggcaagatcg agagcaagat gctgtacctg ggcgaggagg
     8101 tgaagctgct gggcgagggc aagatcaccg gcaagctggt gggcctgagc gagaagggcg
     8161 gcgccctgat cctgaccgag gagggcatca aggagatcct gagcggcgag ttcagcctga
     8221 gaagaagcct tcaactgcct ccacttgaaa gactgacact gggtagttcc tacccatacg
     8281 atgttccaga ttacgctgct agcggcagtg gagagggcag aggaagtctg ctaacatgcg
     8341 gtgacgtcga ggagaatcct ggcccagtga gcaagggcga ggagctgttc accggggtgg
     8401 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     8461 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     8521 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
     8581 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     8641 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     8701 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     8761 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     8821 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     8881 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     8941 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
     9001 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     9061 gcatggacga gctgtacaag taagaattcg atatcaagct tatcgataat caacctctgg
     9121 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     9181 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     9241 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     9301 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     9361 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     9421 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     9481 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     9541 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     9601 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     9661 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgagacc
     9721 tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct
     9781 ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa
     9841 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     9901 tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca
     9961 cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac
    10021 tgacctttgg atggtgctac aagctagtac cagttgagca agagaaggta gaagaagcca
    10081 atgaaggaga gaacacccgc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
    10141 agagagaagt attagagtgg aggtttgaca gccgcctagc atttcatcac atggcccgag
    10201 agctgcatcc ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct
    10261 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
    10321 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
    10381 cagtgtggaa aatctctagc agggcccgtt taaacccgct gatcagcctc gactgtgcct
    10441 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
    10501 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
    10561 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
    10621 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
    10681 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
    10741 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
    10801 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
    10861 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
    10921 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
    10981 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
    11041 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
    11101 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
    11161 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
    11221 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
    11281 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
    11341 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
    11401 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
    11461 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg
    11521 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa
    11581 ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg
    11641 agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt
    11701 cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt
    11761 gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg
    11821 gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg
    11881 cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt
    11941 cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta
    12001 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
    12061 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
    12121 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
    12181 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
    12241 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
    12301 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
    12361 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
    12421 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
    12481 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
    12541 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
    12601 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
    12661 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
    12721 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
    12781 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
    12841 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
    12901 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
    12961 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
    13021 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
    13081 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
    13141 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
    13201 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
    13261 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
    13321 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
    13381 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
    13441 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
    13501 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
    13561 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
    13621 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
    13681 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
    13741 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
    13801 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    13861 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    13921 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    13981 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    14041 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    14101 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    14161 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    14221 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    14281 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    14341 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    14401 aaaagtgcca cctgac
//
